RLG00000017371

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005523
Physical Location & Seq
Forward (+)
15831792 .. 15832486
695 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017371

Sequence Viewer

Length: 408 bp
ATGAAAGAAGGTCCCAGCAAAAGCAACAAGATAGAAGAGGAGGGACAGAAATATGTCAAGTACAGAGGAGTAAGAAGAAGGCCATGGGGAAAGTTTGCAGCAGAAATACGCGATTCGACAAGGCAAGGAGTTCGGTTGTGGCTTGGGACTTTTAATACTGCAAAAGAGGCTGCGAGGGCATATGACAAAGCCGCTTTCTCTATGAGGGGTCCTCTTGCAATTCTCAATTTCCCTAGTGAGCATGGTTTTGTAGATGCTGATTCTGCTGCTACTTCCTCATCTTTCACAGCATCATCTTCAACACTGCATAACATGGAAGTGACAATGGGAACAGAACCCAGAAAAGAAGTTTTTGTGTTCGAGTATCTAGATGACAAGGAGCAACTGTCAAAGAAACTACGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

136

Amino Acids

15.22

Weight (kDa)

9.52

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 20 - 70 1.6e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 111
AciI CCGC 1 cut(s) 192
AfaI GTAC 1 cut(s) 62
AgsI TTSAA 1 cut(s) 300
AoxI GGCC 1 cut(s) 80
ApeKI GCWGC 3 cut(s) 98, 170, 266
AspS9I GGNCC 2 cut(s) 11, 209
AvaII GGWCC 2 cut(s) 11, 209
BbvI GCAGC 3 cut(s) 110, 157, 253
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BfaI CTAG 2 cut(s) 234, 368
BisI GCNGC 4 cut(s) 99, 171, 192, 267
BlsI GCNGC 4 cut(s) 100, 172, 193, 268
Bme18I GGWCC 2 cut(s) 11, 209
BmgT120I GGNCC 2 cut(s) 11, 209
BmiI GGNNCC 2 cut(s) 13, 210
BmsI GCATC 2 cut(s) 244, 299
BplI GAGNNNNNCTC 2 cut(s) 196, 228
BsaAI YACGTR 1 cut(s) 401
BsaJI CCNNGG 1 cut(s) 83
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
BseDI CCNNGG 1 cut(s) 83
BseRI GAGGAG 2 cut(s) 53, 81
BseXI GCAGC 3 cut(s) 110, 157, 253
BseYI CCCAGC 1 cut(s) 14
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 1 cut(s) 82
BslFI GGGAC 2 cut(s) 57, 160
BsmFI GGGAC 2 cut(s) 57, 160
BsnI GGCC 1 cut(s) 82
Bsp19I CCATGG 1 cut(s) 83
BspACI CCGC 1 cut(s) 192
BspANI GGCC 1 cut(s) 82
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 2 cut(s) 13, 210
BssECI CCNNGG 1 cut(s) 83
BssT1I CCWWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 387
Bst6I CTCTTC 1 cut(s) 30
BstBAI YACGTR 1 cut(s) 401
BstDSI CCRYGG 1 cut(s) 83
BstFNI CGCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 3 cut(s) 167, 176, 263
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 3 cut(s) 110, 157, 253
BsuRI GGCC 1 cut(s) 82
BtgI CCRYGG 1 cut(s) 83
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 1 cut(s) 302
Cfr13I GGNCC 2 cut(s) 11, 209
Csp6I GTAC 1 cut(s) 61
CviAII CATG 3 cut(s) 84, 242, 313
CviJI RGCY 4 cut(s) 82, 142, 170, 191
CviKI_1 RGCY 4 cut(s) 82, 142, 170, 191
CviQI GTAC 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 83
Eco47I GGWCC 2 cut(s) 11, 209
EcoO109I RGGNCCY 2 cut(s) 11, 209
EcoT14I CCWWGG 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 83
FaeI CATG 3 cut(s) 87, 245, 316
FaiI YATR 8 cut(s) 54, 85, 181, 183, 203, 243, 309, 314
FaqI GGGAC 2 cut(s) 57, 160
FatI CATG 3 cut(s) 83, 241, 312
FauNDI CATATG 1 cut(s) 181
Fnu4HI GCNGC 4 cut(s) 99, 171, 192, 267
Fsp4HI GCNGC 4 cut(s) 99, 171, 192, 267
FspBI CTAG 2 cut(s) 234, 368
GluI GCNGC 4 cut(s) 99, 171, 192, 267
GsaI CCCAGC 1 cut(s) 18
HaeIII GGCC 1 cut(s) 82
Hin1II CATG 3 cut(s) 87, 245, 316
HinfI GANTC 2 cut(s) 113, 260
Hpy188III TCNNGA 1 cut(s) 368
HpyAV CCTTC 1 cut(s) 72
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4IV ACGT 1 cut(s) 400
HpyCH4V TGCA 4 cut(s) 98, 161, 218, 307
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 176, 263
HpySE526I ACGT 1 cut(s) 400
Hsp92II CATG 3 cut(s) 87, 245, 316
LmnI GCTCC 1 cut(s) 379
LpnPI CCDG 2 cut(s) 28, 352
Lsp1109I GCAGC 3 cut(s) 110, 157, 253
LweI GCATC 2 cut(s) 244, 299
MaeI CTAG 2 cut(s) 234, 368
MaeII ACGT 1 cut(s) 400
MaeIII GTNAC 1 cut(s) 319
MboII GAAGA 3 cut(s) 47, 87, 288
MluCI AATT 2 cut(s) 219, 226
MnlI CCTC 8 cut(s) 31, 34, 59, 160, 168, 198, 222, 286
MseI TTAA 1 cut(s) 153
MslI CAYNNNNRTG 1 cut(s) 317
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 3 cut(s) 167, 176, 263
NcoI CCATGG 1 cut(s) 83
NdeI CATATG 1 cut(s) 181
NlaIII CATG 3 cut(s) 87, 245, 316
NlaIV GGNNCC 2 cut(s) 13, 210
NmuCI GTSAC 1 cut(s) 319
PfeI GAWTC 2 cut(s) 113, 260
PkrI GCNGC 4 cut(s) 100, 172, 193, 268
Ppu21I YACGTR 1 cut(s) 401
PpuMI RGGWCCY 2 cut(s) 11, 209
Psp5II RGGWCCY 2 cut(s) 11, 209
PspFI CCCAGC 1 cut(s) 14
PspN4I GGNNCC 2 cut(s) 13, 210
PspPI GGNCC 2 cut(s) 11, 209
PspPPI RGGWCCY 2 cut(s) 11, 209
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
RseI CAYNNNNRTG 1 cut(s) 317
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 4 cut(s) 99, 171, 192, 267
Sau96I GGNCC 2 cut(s) 11, 209
SetI ASST 2 cut(s) 13, 403
SfaNI GCATC 2 cut(s) 244, 299
SinI GGWCC 2 cut(s) 11, 209
SmiMI CAYNNNNRTG 1 cut(s) 317
Sse9I AATT 2 cut(s) 219, 226
SsiI CCGC 1 cut(s) 192
SspMI CTAG 2 cut(s) 234, 368
StyI CCWWGG 1 cut(s) 83
TaaI ACNGT 1 cut(s) 387
TaiI ACGT 1 cut(s) 403
TaqI TCGA 2 cut(s) 116, 360
TasI AATT 2 cut(s) 219, 226
TatI WGTACW 1 cut(s) 60
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 2 cut(s) 113, 260
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 1 cut(s) 309
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 3 cut(s) 98, 170, 266
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 309
VpaK11BI GGWCC 2 cut(s) 11, 209
XbaI TCTAGA 1 cut(s) 367
XspI CTAG 2 cut(s) 234, 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.