Rroxscaffold_7G00180270

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005523
Physical Location & Seq
Forward (+)
19733749 .. 19736174
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00180270.1

Sequence Viewer

Length: 609 bp
ATGTTGCCTACCCTCTCACTGCTGAGATTGTGTGCAGGGGACATTTCACCATTTCGGCAGATGAAGATGCCAGTGCTGCGCAATTGGTACCAGACTAAAGTAGTGAAACGAATATCAAATTTTCACTCAGAAGTACTATTAGGCCACATGGAAGGAGACTCAAGTAGAGGCAGCAAGGCAAAACAAGAGGGACAGAAAGATGTCAAGTACAGAGGAGTGAGGAGAAGGCCATGGGGAAAGTTCGCAGCAGAAATACGCGATTCAACGAGGCATGGAGAAGGAGCTCGGTTGTGGCTCGGGACTTTCAACACTGCAGAAGAGGCTGCAAGGGCTTATGACAGTGCTGCTTTTTCGATGAGGGGTGCTCTTGCAATTCTCAACTTCCCACGTGAGCATGGTTTGCCAGATAGTTCTGCTGTTTCTGCTGTTTCCGCTGTTTCCTCATCATCTTCATCTTCGTCATCCCCAACATCCATTTCAGCATCATCTTCAACATTGCAGAATGTGGAAGGCACAACTAGGACAGAACCCAGAAGAGAAGTCTTCGAAATTGAGTGTTTAGATGACAAGGTTTTAGAGGACCTTCTTGATTTTGACAGGAAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

202

Amino Acids

22.33

Weight (kDa)

9.19

Isoelectric Point (pI)

62.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 69 - 121 1.6e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 80
Acc65I GGTACC 1 cut(s) 87
AccB1I GGYRCC 1 cut(s) 87
AccII CGCG 1 cut(s) 258
AciI CCGC 1 cut(s) 432
AcsI RAATTY 1 cut(s) 118
AcvI CACGTG 1 cut(s) 389
AfaI GTAC 3 cut(s) 89, 135, 209
AgsI TTSAA 3 cut(s) 264, 307, 492
AjuI GAANNNNNNNTTGG 2 cut(s) 460, 492
AluBI AGCT 1 cut(s) 284
AluI AGCT 1 cut(s) 284
Alw21I GWGCWC 2 cut(s) 286, 367
Alw26I GTCTC 1 cut(s) 150
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 2 cut(s) 142, 227
ApeKI GCWGC 5 cut(s) 76, 171, 245, 323, 344
ApoI RAATTY 1 cut(s) 118
Asp718I GGTACC 1 cut(s) 87
AspLEI GCGC 1 cut(s) 81
AspS9I GGNCC 1 cut(s) 580
AsuHPI GGTGA 1 cut(s) 39
AsuII TTCGAA 1 cut(s) 546
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 1 cut(s) 580
BanI GGYRCC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 286
BbrPI CACGTG 1 cut(s) 389
BbsI GAAGAC 1 cut(s) 535
Bbv12I GWGCWC 2 cut(s) 286, 367
BbvI GCAGC 5 cut(s) 63, 183, 257, 310, 331
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 2 cut(s) 519, 607
BfmI CTRYAG 1 cut(s) 312
BisI GCNGC 5 cut(s) 77, 172, 246, 324, 345
BlsI GCNGC 5 cut(s) 78, 173, 247, 325, 346
BmcAI AGTACT 1 cut(s) 135
Bme18I GGWCC 1 cut(s) 580
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 580
BmiI GGNNCC 1 cut(s) 89
BmsI GCATC 2 cut(s) 57, 491
BpiI GAAGAC 1 cut(s) 535
BplI GAGNNNNNCTC 2 cut(s) 349, 381
Bpu14I TTCGAA 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 145
BsaAI YACGTR 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 230
Bse1I ACTGG 1 cut(s) 71
Bse3DI GCAATG 1 cut(s) 494
BseDI CCNNGG 1 cut(s) 230
BseGI GGATG 2 cut(s) 461, 470
BseMI GCAATG 1 cut(s) 494
BseMII CTCAG 2 cut(s) 14, 141
BseNI ACTGG 1 cut(s) 71
BseRI GAGGAG 2 cut(s) 228, 235
BseXI GCAGC 5 cut(s) 63, 183, 257, 310, 331
BsgI GTGCAG 1 cut(s) 54
Bsh1236I CGCG 1 cut(s) 258
BshFI GGCC 2 cut(s) 144, 229
BshNI GGYRCC 1 cut(s) 87
BsiHKAI GWGCWC 2 cut(s) 286, 367
BsiHKCI CYCGRG 1 cut(s) 296
BslFI GGGAC 3 cut(s) 53, 204, 313
BsmAI GTCTC 1 cut(s) 150
BsmFI GGGAC 3 cut(s) 53, 204, 313
BsnI GGCC 2 cut(s) 144, 229
BsoBI CYCGRG 1 cut(s) 296
Bsp119I TTCGAA 1 cut(s) 546
Bsp1286I GDGCHC 2 cut(s) 286, 367
Bsp19I CCATGG 1 cut(s) 230
BspACI CCGC 1 cut(s) 432
BspANI GGCC 2 cut(s) 144, 229
BspCNI CTCAG 2 cut(s) 15, 140
BspFNI CGCG 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 89
BspMAI CTGCAG 1 cut(s) 316
BspT104I TTCGAA 1 cut(s) 546
BspT107I GGYRCC 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 494
BsrI ACTGG 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 230
BssT1I CCWWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 341
Bst6I CTCTTC 2 cut(s) 312, 529
BstAPI GCANNNNNTGC 1 cut(s) 400
BstBAI YACGTR 1 cut(s) 389
BstBI TTCGAA 1 cut(s) 546
BstDEI CTNAG 2 cut(s) 23, 127
BstDSI CCRYGG 1 cut(s) 230
BstF5I GGATG 2 cut(s) 461, 470
BstFNI CGCG 1 cut(s) 258
BstHHI GCGC 1 cut(s) 81
BstMAI GTCTC 1 cut(s) 150
BstMWI GCNNNNNNNGC 6 cut(s) 76, 320, 329, 400, 422, 431
BstSFI CTRYAG 1 cut(s) 312
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 5 cut(s) 63, 183, 257, 310, 331
BstV2I GAAGAC 1 cut(s) 535
BsuRI GGCC 2 cut(s) 144, 229
BtgI CCRYGG 1 cut(s) 230
BtsCI GGATG 2 cut(s) 461, 470
BtsI GCAGTG 2 cut(s) 17, 309
BtsIMutI CAGTG 4 cut(s) 17, 78, 309, 346
CfoI GCGC 1 cut(s) 81
Cfr13I GGNCC 1 cut(s) 580
Csp6I GTAC 3 cut(s) 88, 134, 208
CviAII CATG 4 cut(s) 148, 231, 272, 395
CviJI RGCY 7 cut(s) 144, 229, 284, 295, 323, 332, 606
CviKI_1 RGCY 7 cut(s) 144, 229, 284, 295, 323, 332, 606
CviQI GTAC 3 cut(s) 88, 134, 208
DdeI CTNAG 2 cut(s) 23, 127
Eam1104I CTCTTC 2 cut(s) 312, 529
EarI CTCTTC 2 cut(s) 312, 529
Ecl136II GAGCTC 1 cut(s) 284
Eco130I CCWWGG 1 cut(s) 230
Eco24I GRGCYC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 580
Eco53kI GAGCTC 1 cut(s) 284
Eco72I CACGTG 1 cut(s) 389
Eco88I CYCGRG 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 580
EcoT14I CCWWGG 1 cut(s) 230
EcoT38I GRGCYC 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 230
FaeI CATG 4 cut(s) 151, 234, 275, 398
FaiI YATR 5 cut(s) 149, 232, 273, 336, 396
FaqI GGGAC 3 cut(s) 53, 204, 313
FatI CATG 4 cut(s) 147, 230, 271, 394
Fnu4HI GCNGC 5 cut(s) 77, 172, 246, 324, 345
FokI GGATG 2 cut(s) 448, 457
FriOI GRGCYC 1 cut(s) 286
Fsp4HI GCNGC 5 cut(s) 77, 172, 246, 324, 345
FspBI CTAG 2 cut(s) 519, 607
FspI TGCGCA 1 cut(s) 80
GlaI GCGC 1 cut(s) 80
GluI GCNGC 5 cut(s) 77, 172, 246, 324, 345
HaeIII GGCC 2 cut(s) 144, 229
HhaI GCGC 1 cut(s) 81
Hin1II CATG 4 cut(s) 151, 234, 275, 398
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HinfI GANTC 2 cut(s) 158, 260
HphI GGTGA 1 cut(s) 39
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 2 cut(s) 298, 587
HpyAV CCTTC 5 cut(s) 146, 219, 272, 503, 593
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 5 cut(s) 35, 314, 326, 371, 499
HpyF10VI GCNNNNNNNGC 6 cut(s) 76, 320, 329, 400, 422, 431
HpyF3I CTNAG 2 cut(s) 23, 127
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 4 cut(s) 151, 234, 275, 398
HspAI GCGC 1 cut(s) 79
KpnI GGTACC 1 cut(s) 91
LmnI GCTCC 1 cut(s) 281
LpnPI CCDG 6 cut(s) 21, 84, 104, 417, 544, 583
Lsp1109I GCAGC 5 cut(s) 63, 183, 257, 310, 331
LweI GCATC 2 cut(s) 57, 491
MaeI CTAG 2 cut(s) 519, 607
MaeII ACGT 1 cut(s) 388
MboII GAAGA 7 cut(s) 76, 329, 441, 447, 480, 535, 546
MfeI CAATTG 1 cut(s) 82
MhlI GDGCHC 2 cut(s) 286, 367
MluCI AATT 4 cut(s) 82, 118, 372, 549
MlyI GAGTC 1 cut(s) 152
MspA1I CMGCKG 1 cut(s) 434
MunI CAATTG 1 cut(s) 82
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 6 cut(s) 76, 320, 329, 400, 422, 431
NcoI CCATGG 1 cut(s) 230
NlaIII CATG 4 cut(s) 151, 234, 275, 398
NlaIV GGNNCC 1 cut(s) 89
NsbI TGCGCA 1 cut(s) 80
NspV TTCGAA 1 cut(s) 546
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 5 cut(s) 78, 173, 247, 325, 346
PleI GAGTC 1 cut(s) 152
PmaCI CACGTG 1 cut(s) 389
PmlI CACGTG 1 cut(s) 389
PpsI GAGTC 1 cut(s) 152
Ppu21I YACGTR 1 cut(s) 389
PpuMI RGGWCCY 1 cut(s) 580
Psp124BI GAGCTC 1 cut(s) 286
Psp5II RGGWCCY 1 cut(s) 580
PspCI CACGTG 1 cut(s) 389
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 1 cut(s) 580
PspPPI RGGWCCY 1 cut(s) 580
PstI CTGCAG 1 cut(s) 316
RsaI GTAC 3 cut(s) 89, 135, 209
RsaNI GTAC 3 cut(s) 88, 134, 208
SacI GAGCTC 1 cut(s) 286
SatI GCNGC 5 cut(s) 77, 172, 246, 324, 345
Sau96I GGNCC 1 cut(s) 580
ScaI AGTACT 1 cut(s) 135
SchI GAGTC 1 cut(s) 152
SduI GDGCHC 2 cut(s) 286, 367
SetI ASST 4 cut(s) 286, 391, 573, 585
SfaNI GCATC 2 cut(s) 57, 491
SfcI CTRYAG 1 cut(s) 312
SfuI TTCGAA 1 cut(s) 546
SinI GGWCC 1 cut(s) 580
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
Sse9I AATT 4 cut(s) 82, 118, 372, 549
SsiI CCGC 1 cut(s) 432
SspMI CTAG 2 cut(s) 519, 607
SstI GAGCTC 1 cut(s) 286
StyI CCWWGG 1 cut(s) 230
TaaI ACNGT 1 cut(s) 341
TaiI ACGT 1 cut(s) 391
TaqI TCGA 2 cut(s) 353, 546
TasI AATT 4 cut(s) 82, 118, 372, 549
TatI WGTACW 2 cut(s) 133, 207
TfiI GAWTC 1 cut(s) 260
TscAI CASTG 4 cut(s) 24, 78, 316, 346
TseI GCWGC 5 cut(s) 76, 171, 245, 323, 344
TspDTI ATGAA 2 cut(s) 77, 441
TspRI CASTG 4 cut(s) 24, 78, 316, 346
VpaK11BI GGWCC 1 cut(s) 580
XapI RAATTY 1 cut(s) 118
XspI CTAG 2 cut(s) 519, 607
ZrmI AGTACT 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.