pycom05g18520

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005523
Physical Location & Seq
Reverse (-)
21459594 .. 21460046
453 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18520.1

Sequence Viewer

Length: 453 bp
ATGGAAAGAGGCGGCAAAGATAAAGAAGAGAGACAGAAAGATATTAAGTATAGAGGTGTGAGAACAAGGCCGTGGGGGAAATTCGCAGCCGAAATACGCGATTCGACGAGGCAGGGAGCTCGGTTGTGGCTTGGCACCTTCAACACTGCTGAAGAAGCTGCAAGGGCTTATGATAGAGCTGCTTTTTCCATGAGGGGTCCTCTTGCAATCCTCAATTTTCCAAGTGAATATGATTTGAAAGATGCTGGTCAGTCTGCTGCTGCTGCTACTACTTCCTCGTCAACTTCTTCGTCTTCATCTTCTTTGTCGTCGTCTTCAAGACCCCATAATGTGACCAGGACGGAATCCGGAAGAGAAATCTTCGAGTTTGAGTGTTTAGATGATAGCGTTTTAGAGGACCTTCTTGATTTTGACGATCGTACCAAAGCTAATGAAGAAAAACAAATTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

151

Amino Acids

16.77

Weight (kDa)

5.58

Isoelectric Point (pI)

55.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 16 - 66 7e-16 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 134
AccII CGCG 1 cut(s) 99
AccIII TCCGGA 1 cut(s) 347
AciI CCGC 1 cut(s) 12
AcsI RAATTY 1 cut(s) 80
AcuI CTGAAG 1 cut(s) 171
AfaI GTAC 1 cut(s) 421
AgsI TTSAA 3 cut(s) 142, 238, 318
AjnI CCWGG 1 cut(s) 335
AluBI AGCT 4 cut(s) 119, 158, 179, 428
AluI AGCT 4 cut(s) 119, 158, 179, 428
Alw21I GWGCWC 1 cut(s) 121
Alw26I GTCTC 1 cut(s) 25
Aor13HI TCCGGA 1 cut(s) 347
AoxI GGCC 1 cut(s) 68
ApeKI GCWGC 6 cut(s) 86, 158, 179, 257, 260, 263
ApoI RAATTY 1 cut(s) 80
AspS9I GGNCC 2 cut(s) 197, 397
AvaII GGWCC 2 cut(s) 197, 397
BaeI ACNNNNGTAYC 2 cut(s) 403, 436
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 121
BbsI GAAGAC 2 cut(s) 285, 306
Bbv12I GWGCWC 1 cut(s) 121
BbvI GCAGC 6 cut(s) 98, 145, 166, 244, 247, 250
BceAI ACGGC 1 cut(s) 55
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 1 cut(s) 337
BcoDI GTCTC 1 cut(s) 25
BisI GCNGC 7 cut(s) 13, 87, 159, 180, 258, 261, 264
BlsI GCNGC 7 cut(s) 14, 88, 160, 181, 259, 262, 265
Bme1390I CCNGG 1 cut(s) 337
Bme18I GGWCC 2 cut(s) 197, 397
BmgT120I GGNCC 2 cut(s) 197, 397
BmiI GGNNCC 2 cut(s) 136, 198
BmrFI CCNGG 1 cut(s) 337
BmsI GCATC 1 cut(s) 232
BpiI GAAGAC 2 cut(s) 285, 306
BplI GAGNNNNNCTC 2 cut(s) 184, 216
BsaJI CCNNGG 1 cut(s) 71
BsaWI WCCGGW 1 cut(s) 347
BseAI TCCGGA 1 cut(s) 347
BseBI CCWGG 1 cut(s) 337
BseDI CCNNGG 1 cut(s) 71
BseXI GCAGC 6 cut(s) 98, 145, 166, 244, 247, 250
Bsh1236I CGCG 1 cut(s) 99
Bsh1285I CGRYCG 1 cut(s) 418
BshFI GGCC 1 cut(s) 70
BshNI GGYRCC 1 cut(s) 134
BsiEI CGRYCG 1 cut(s) 418
BsiHKAI GWGCWC 1 cut(s) 121
BsiSI CCGG 1 cut(s) 348
BsmAI GTCTC 1 cut(s) 25
BsnI GGCC 1 cut(s) 70
Bsp1286I GDGCHC 1 cut(s) 121
Bsp13I TCCGGA 1 cut(s) 347
Bsp143I GATC 1 cut(s) 415
BspACI CCGC 1 cut(s) 12
BspANI GGCC 1 cut(s) 70
BspEI TCCGGA 1 cut(s) 347
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 2 cut(s) 136, 198
BspT107I GGYRCC 1 cut(s) 134
BssECI CCNNGG 1 cut(s) 71
BssMI GATC 1 cut(s) 415
Bst2UI CCWGG 1 cut(s) 337
Bst6I CTCTTC 2 cut(s) 21, 346
BstDSI CCRYGG 1 cut(s) 71
BstFNI CGCG 1 cut(s) 99
BstKTI GATC 1 cut(s) 418
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 1 cut(s) 415
BstMCI CGRYCG 1 cut(s) 418
BstMWI GCNNNNNNNGC 3 cut(s) 155, 164, 263
BstNI CCWGG 1 cut(s) 337
BstSCI CCNGG 1 cut(s) 335
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 6 cut(s) 98, 145, 166, 244, 247, 250
BstV2I GAAGAC 2 cut(s) 285, 306
BsuRI GGCC 1 cut(s) 70
BtgI CCRYGG 1 cut(s) 71
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 197, 397
Csp6I GTAC 1 cut(s) 420
CviAII CATG 1 cut(s) 190
CviJI RGCY 8 cut(s) 70, 89, 119, 130, 158, 167, 179, 428
CviKI_1 RGCY 8 cut(s) 70, 89, 119, 130, 158, 167, 179, 428
CviQI GTAC 1 cut(s) 420
DpnI GATC 1 cut(s) 417
DpnII GATC 1 cut(s) 415
Eam1104I CTCTTC 2 cut(s) 21, 346
EarI CTCTTC 2 cut(s) 21, 346
Ecl136II GAGCTC 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 121
Eco47I GGWCC 2 cut(s) 197, 397
Eco53kI GAGCTC 1 cut(s) 119
Eco57I CTGAAG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 119
EcoO109I RGGNCCY 2 cut(s) 197, 397
EcoRII CCWGG 1 cut(s) 335
EcoT38I GRGCYC 1 cut(s) 121
FaeI CATG 1 cut(s) 193
FaiI YATR 5 cut(s) 51, 171, 191, 231, 327
FatI CATG 1 cut(s) 189
Fnu4HI GCNGC 7 cut(s) 13, 87, 159, 180, 258, 261, 264
FriOI GRGCYC 1 cut(s) 121
Fsp4HI GCNGC 7 cut(s) 13, 87, 159, 180, 258, 261, 264
GluI GCNGC 7 cut(s) 13, 87, 159, 180, 258, 261, 264
HaeIII GGCC 1 cut(s) 70
HapII CCGG 1 cut(s) 348
Hin1II CATG 1 cut(s) 193
HincII GTYRAC 1 cut(s) 282
HindII GTYRAC 1 cut(s) 282
HinfI GANTC 2 cut(s) 101, 344
HpaII CCGG 1 cut(s) 348
Hpy166II GTNNAC 1 cut(s) 282
Hpy188III TCNNGA 3 cut(s) 318, 348, 404
Hpy8I GTNNAC 1 cut(s) 282
Hpy99I CGWCG 2 cut(s) 109, 313
HpyAV CCTTC 2 cut(s) 148, 410
HpyCH4V TGCA 2 cut(s) 161, 206
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 164, 263
Hsp92II CATG 1 cut(s) 193
Kpn2I TCCGGA 1 cut(s) 347
Kzo9I GATC 1 cut(s) 415
LmnI GCTCC 1 cut(s) 116
LpnPI CCDG 5 cut(s) 98, 231, 322, 349, 361
Lsp1109I GCAGC 6 cut(s) 98, 145, 166, 244, 247, 250
LweI GCATC 1 cut(s) 232
MaeIII GTNAC 1 cut(s) 331
MalI GATC 1 cut(s) 417
MboI GATC 1 cut(s) 415
MboII GAAGA 9 cut(s) 38, 164, 279, 285, 291, 306, 352, 363, 446
MhlI GDGCHC 1 cut(s) 121
MluCI AATT 3 cut(s) 80, 214, 444
MnlI CCTC 7 cut(s) 47, 102, 186, 210, 221, 286, 388
MroI TCCGGA 1 cut(s) 347
MseI TTAA 2 cut(s) 45, 447
MspI CCGG 1 cut(s) 348
MspR9I CCNGG 1 cut(s) 337
MvaI CCWGG 1 cut(s) 337
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 3 cut(s) 155, 164, 263
NdeII GATC 1 cut(s) 415
NlaIII CATG 1 cut(s) 193
NlaIV GGNNCC 2 cut(s) 136, 198
NmuCI GTSAC 1 cut(s) 331
PfeI GAWTC 2 cut(s) 101, 344
PkrI GCNGC 7 cut(s) 14, 88, 160, 181, 259, 262, 265
Ple19I CGATCG 1 cut(s) 418
PpuMI RGGWCCY 2 cut(s) 197, 397
Psp124BI GAGCTC 1 cut(s) 121
Psp5II RGGWCCY 2 cut(s) 197, 397
Psp6I CCWGG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 335
PspN4I GGNNCC 2 cut(s) 136, 198
PspPI GGNCC 2 cut(s) 197, 397
PspPPI RGGWCCY 2 cut(s) 197, 397
PvuI CGATCG 1 cut(s) 418
RsaI GTAC 1 cut(s) 421
RsaNI GTAC 1 cut(s) 420
SacI GAGCTC 1 cut(s) 121
SaqAI TTAA 2 cut(s) 45, 447
SatI GCNGC 7 cut(s) 13, 87, 159, 180, 258, 261, 264
Sau3AI GATC 1 cut(s) 415
Sau96I GGNCC 2 cut(s) 197, 397
ScrFI CCNGG 1 cut(s) 337
SduI GDGCHC 1 cut(s) 121
SetI ASST 7 cut(s) 58, 121, 140, 160, 181, 402, 430
SfaNI GCATC 1 cut(s) 232
SinI GGWCC 2 cut(s) 197, 397
Sse9I AATT 3 cut(s) 80, 214, 444
SsiI CCGC 1 cut(s) 12
SstI GAGCTC 1 cut(s) 121
StyD4I CCNGG 1 cut(s) 335
TaqI TCGA 2 cut(s) 104, 363
TasI AATT 3 cut(s) 80, 214, 444
TauI GCSGC 1 cut(s) 15
TfiI GAWTC 2 cut(s) 101, 344
Tru1I TTAA 2 cut(s) 45, 447
Tru9I TTAA 2 cut(s) 45, 447
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 6 cut(s) 86, 158, 179, 257, 260, 263
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 2 cut(s) 285, 447
TspGWI ACGGA 1 cut(s) 356
TspRI CASTG 1 cut(s) 151
VpaK11BI GGWCC 2 cut(s) 197, 397
XapI RAATTY 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.