FvH4_2g33452

Domain of unknown function (DUF4793)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002655
Physical Location & Seq
Reverse (-)
25095463 .. 25098081
2619 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33452.t1

Sequence Viewer

Length: 1170 bp
ATGTACAGACCTACGTGGGCGCATTCGACTCCGGCTGGCCCTCAGCGGTGGCAGGAGAGTTGGGCTCGCCTGCTTACTCCTTTGACCCTTTGGATATGTGTATCAGTGAGCATTCGATATGGTTACTATGGAGATAACCGCATCACGCTTGGCCCGAGCTCGTCTCGCATGATCAAGGCTAGTTCTGTTTTTGTACAACAAGTTGAAGTGAGAGATGTTGACAAGAAAGGGGTTTCTCTCTATGCTCTTTCTGAAGAGCCTAAGTTGAGCCAACAGGTGAATTGGAATGTGTCTAACTACTTGATCATTGGGGCCTATAATCGCAAGGGATATTCTTTCTGGTTAAACAAGGGTTCAAGTATATCCATGAGATTGGAAGCTCACCCTACTCATGTAAATCAACTCCAAGTTGTCATGCTTAAAGGGGAAAGGAAACTTGAGATACTGCAGCCGAAACAAACAAGTTCTGTGAATTCCTATAATGAATCCGAAATTGGTAAAGAAGCTGAATTTAGCATTGAGGAAGATGACAGGTACTACCTTGACATCACAAACACAAATCCTAAAAGCATAATCATAACAGTGAATGTAAATGTCTCCTCAAAGATGTATGATACTACGAAAGCTAAGGCCATCTGCTCGACAGCAACTAAAGGTTCTTCGTGCCAGCTCAGGCTTACATTCCCCAATACTCACTATGTCATACTGACGACACCTAACAATGGGGATCTTCTTGGATGGTACATCGAGCTTTCTTTTGTGGCTCGTGTGATAACATACATTGGAATTTTAGGGTTTATAGTGGTCATGATCTTTCTAATATTGAAGTACCTCGGCGCCTGTGATGTTGAGAGTAGTACTGTAGTAGAACACTCAAGACAAGAAATTAGTGAGACTTACCCTATAGTGCCGCCCAAGACAGTTCCACAAACATATGGTACAAATGAAGAAGATGATGATTCGGGAGCTTCGAGTAGCTCCTCAGAGGAATTATATGATGAGAAGCTATGCGTGATATGTTATGATGAGCAGCGTAACTGCTTCTTTGTTCCTTGTGGTCACTGTGCAACCTGCTACGACTGTGCTCAGAGGATTATGGATGGGGAAAGTAAGGTGTGTCCCATATGTAGAAGGCTCATTCACAAAGTGAGAAGATTGTTTCACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4792 DUF4793 zf-C3HC4_3

Protein Analysis

390

Amino Acids

44.08

Weight (kDa)

6.71

Isoelectric Point (pI)

47.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APD1-4_N PF16040 79 - 146 8.3e-14 E3 ubiquitin-protein ligase APD1-4, N-terminal
APD1-4_M PF16041 169 - 273 1.9e-24 E3 ubiquitin-protein ligase APD1-4, middle domain
zf-C3HC4_3 PF13920 334 - 383 3.4e-15 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 336 - 377 1.6e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1079
AccB1I GGYRCC 1 cut(s) 836
AciI CCGC 3 cut(s) 46, 139, 911
AclWI GGATC 1 cut(s) 735
AcsI RAATTY 3 cut(s) 472, 509, 786
AcuI CTGAAG 1 cut(s) 273
AcyI GRCGYC 1 cut(s) 837
AdeI CACNNNGTG 1 cut(s) 1147
AfaI GTAC 7 cut(s) 5, 195, 536, 743, 830, 859, 940
AfiI CCNNNNNNNGG 1 cut(s) 722
AgsI TTSAA 3 cut(s) 206, 357, 826
AjuI GAANNNNNNNTTGG 2 cut(s) 477, 509
AluBI AGCT 9 cut(s) 159, 380, 506, 626, 670, 751, 968, 978, 1006
AluI AGCT 9 cut(s) 159, 380, 506, 626, 670, 751, 968, 978, 1006
Alw21I GWGCWC 2 cut(s) 161, 1087
Alw26I GTCTC 3 cut(s) 168, 601, 887
AlwI GGATC 1 cut(s) 735
Ama87I CYCGRG 1 cut(s) 154
AoxI GGCC 4 cut(s) 37, 151, 312, 630
ApeKI GCWGC 2 cut(s) 448, 1030
ApoI RAATTY 3 cut(s) 472, 509, 786
AspLEI GCGC 2 cut(s) 22, 839
AspS9I GGNCC 3 cut(s) 38, 152, 312
AsuHPI GGTGA 2 cut(s) 289, 374
AvaI CYCGRG 1 cut(s) 154
BanI GGYRCC 1 cut(s) 836
BanII GRGCYC 2 cut(s) 67, 161
BauI CACGAG 1 cut(s) 765
Bbv12I GWGCWC 2 cut(s) 161, 1087
BbvCI CCTCAGC 1 cut(s) 42
BbvI GCAGC 2 cut(s) 460, 1042
BccI CCATC 3 cut(s) 641, 732, 1094
BclI TGATCA 2 cut(s) 171, 303
BcoDI GTCTC 3 cut(s) 168, 601, 887
BfaI CTAG 1 cut(s) 180
BfmI CTRYAG 3 cut(s) 446, 861, 903
BfoI RGCGCY 1 cut(s) 840
BfuAI ACCTGC 1 cut(s) 1079
BisI GCNGC 3 cut(s) 449, 911, 1031
BlsI GCNGC 3 cut(s) 450, 912, 1032
BmcAI AGTACT 1 cut(s) 859
BmeT110I CYCGRG 1 cut(s) 154
BmgT120I GGNCC 3 cut(s) 38, 152, 312
BmiI GGNNCC 2 cut(s) 313, 838
BmsI GCATC 1 cut(s) 150
BplI GAGNNNNNCTC 4 cut(s) 49, 81, 148, 180
Bpu10I CCTNAGC 3 cut(s) 42, 627, 671
BpuEI CTTGAG 2 cut(s) 458, 859
BsaAI YACGTR 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 837
BsaJI CCNNGG 1 cut(s) 832
Bsc4I CCNNNNNNNGG 1 cut(s) 722
BseDI CCNNGG 1 cut(s) 832
BseGI GGATG 2 cut(s) 743, 1105
BseLI CCNNNNNNNGG 1 cut(s) 722
BseMII CTCAG 4 cut(s) 56, 685, 996, 1100
BseRI GAGGAG 2 cut(s) 589, 970
BseXI GCAGC 2 cut(s) 460, 1042
BshFI GGCC 4 cut(s) 39, 153, 314, 632
BshNI GGYRCC 1 cut(s) 836
BsiHKAI GWGCWC 2 cut(s) 161, 1087
BsiHKCI CYCGRG 1 cut(s) 154
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 1 cut(s) 1104
BslI CCNNNNNNNGG 1 cut(s) 722
BsmAI GTCTC 3 cut(s) 168, 601, 887
BsmBI CGTCTC 1 cut(s) 168
BsmFI GGGAC 1 cut(s) 1104
BsmI GAATGC 2 cut(s) 22, 111
BsnI GGCC 4 cut(s) 39, 153, 314, 632
BsoBI CYCGRG 1 cut(s) 154
Bsp1286I GDGCHC 3 cut(s) 67, 161, 1087
Bsp1407I TGTACA 2 cut(s) 3, 193
Bsp143I GATC 4 cut(s) 171, 303, 727, 810
BspACI CCGC 3 cut(s) 46, 139, 911
BspANI GGCC 4 cut(s) 39, 153, 314, 632
BspCNI CTCAG 4 cut(s) 55, 684, 995, 1099
BspHI TCATGA 1 cut(s) 807
BspLI GGNNCC 2 cut(s) 313, 838
BspMAI CTGCAG 1 cut(s) 450
BspMI ACCTGC 1 cut(s) 1079
BspPI GGATC 1 cut(s) 735
BspQI GCTCTTC 1 cut(s) 249
BspT107I GGYRCC 1 cut(s) 836
BsrGI TGTACA 2 cut(s) 3, 193
BssECI CCNNGG 1 cut(s) 832
BssMI GATC 4 cut(s) 171, 303, 727, 810
BssNI GRCGYC 1 cut(s) 837
BssSI CACGAG 1 cut(s) 765
Bst2BI CACGAG 1 cut(s) 765
Bst4CI ACNGT 5 cut(s) 583, 862, 922, 1064, 1082
Bst6I CTCTTC 1 cut(s) 249
BstACI GRCGYC 1 cut(s) 837
BstAUI TGTACA 2 cut(s) 3, 193
BstBAI YACGTR 1 cut(s) 15
BstC8I GCNNGC 4 cut(s) 37, 67, 71, 668
BstDEI CTNAG 6 cut(s) 42, 261, 627, 671, 982, 1086
BstF5I GGATG 2 cut(s) 743, 1105
BstH2I RGCGCY 1 cut(s) 840
BstHHI GCGC 2 cut(s) 22, 839
BstKTI GATC 4 cut(s) 174, 306, 730, 813
BstMAI GTCTC 3 cut(s) 168, 601, 887
BstMBI GATC 4 cut(s) 171, 303, 727, 810
BstMWI GCNNNNNNNGC 1 cut(s) 165
BstSFI CTRYAG 3 cut(s) 446, 861, 903
BstV1I GCAGC 2 cut(s) 460, 1042
BstX2I RGATCY 1 cut(s) 727
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 1 cut(s) 727
BsuRI GGCC 4 cut(s) 39, 153, 314, 632
BtsCI GGATG 2 cut(s) 743, 1105
BtsIMutI CAGTG 3 cut(s) 111, 588, 1060
BveI ACCTGC 1 cut(s) 1079
Cac8I GCNNGC 4 cut(s) 37, 67, 71, 668
CciI TCATGA 1 cut(s) 807
CfoI GCGC 2 cut(s) 22, 839
Cfr13I GGNCC 3 cut(s) 38, 152, 312
Csp6I GTAC 7 cut(s) 4, 194, 535, 742, 829, 858, 939
CviAII CATG 5 cut(s) 169, 367, 392, 415, 808
CviQI GTAC 7 cut(s) 4, 194, 535, 742, 829, 858, 939
DdeI CTNAG 6 cut(s) 42, 261, 627, 671, 982, 1086
DinI GGCGCC 1 cut(s) 838
DpnI GATC 4 cut(s) 173, 305, 729, 812
DpnII GATC 4 cut(s) 171, 303, 727, 810
DraIII CACNNNGTG 1 cut(s) 1147
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Ecl136II GAGCTC 1 cut(s) 159
Eco24I GRGCYC 2 cut(s) 67, 161
Eco53kI GAGCTC 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 154
EcoICRI GAGCTC 1 cut(s) 159
EcoO109I RGGNCCY 1 cut(s) 312
EcoRI GAATTC 1 cut(s) 472
EcoT38I GRGCYC 2 cut(s) 67, 161
EgeI GGCGCC 1 cut(s) 838
EheI GGCGCC 1 cut(s) 838
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 5 cut(s) 172, 370, 395, 418, 811
FaqI GGGAC 1 cut(s) 1104
FatI CATG 5 cut(s) 168, 366, 391, 414, 807
FauNDI CATATG 2 cut(s) 934, 1124
FbaI TGATCA 2 cut(s) 171, 303
Fnu4HI GCNGC 3 cut(s) 449, 911, 1031
FokI GGATG 2 cut(s) 750, 1112
FriOI GRGCYC 2 cut(s) 67, 161
Fsp4HI GCNGC 3 cut(s) 449, 911, 1031
FspBI CTAG 1 cut(s) 180
GlaI GCGC 2 cut(s) 21, 838
GluI GCNGC 3 cut(s) 449, 911, 1031
HaeII RGCGCY 1 cut(s) 840
HaeIII GGCC 4 cut(s) 39, 153, 314, 632
HapII CCGG 1 cut(s) 32
HhaI GCGC 2 cut(s) 22, 839
Hin1I GRCGYC 1 cut(s) 837
Hin1II CATG 5 cut(s) 172, 370, 395, 418, 811
Hin6I GCGC 2 cut(s) 20, 837
HinP1I GCGC 2 cut(s) 20, 837
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 3 cut(s) 28, 485, 959
HpaII CCGG 1 cut(s) 32
HphI GGTGA 2 cut(s) 289, 374
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 4 cut(s) 253, 490, 985, 1089
Hpy188III TCNNGA 3 cut(s) 808, 876, 963
Hpy8I GTNNAC 1 cut(s) 220
HpyAV CCTTC 1 cut(s) 1125
HpyCH4III ACNGT 5 cut(s) 583, 862, 922, 1064, 1082
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 2 cut(s) 448, 1067
HpyF10VI GCNNNNNNNGC 1 cut(s) 165
HpyF3I CTNAG 6 cut(s) 42, 261, 627, 671, 982, 1086
HpySE526I ACGT 1 cut(s) 14
Hsp92I GRCGYC 1 cut(s) 837
Hsp92II CATG 5 cut(s) 172, 370, 395, 418, 811
HspAI GCGC 2 cut(s) 20, 837
KasI GGCGCC 1 cut(s) 836
Ksp22I TGATCA 2 cut(s) 171, 303
Kzo9I GATC 4 cut(s) 171, 303, 727, 810
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 2 cut(s) 965, 983
Lsp1109I GCAGC 2 cut(s) 460, 1042
LweI GCATC 1 cut(s) 150
MaeI CTAG 1 cut(s) 180
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 3 cut(s) 122, 1034, 1058
MalI GATC 4 cut(s) 173, 305, 729, 812
MboI GATC 4 cut(s) 171, 303, 727, 810
MboII GAAGA 7 cut(s) 266, 536, 651, 722, 959, 962, 1164
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 3 cut(s) 67, 161, 1087
MluCI AATT 7 cut(s) 280, 472, 492, 509, 786, 885, 989
Mly113I GGCGCC 1 cut(s) 837
MlyI GAGTC 1 cut(s) 22
MnlI CCTC 7 cut(s) 51, 514, 610, 842, 979, 991, 1083
MseI TTAA 3 cut(s) 344, 420, 1168
MslI CAYNNNNRTG 1 cut(s) 581
MspA1I CMGCKG 1 cut(s) 46
MspI CCGG 1 cut(s) 32
Mva1269I GAATGC 2 cut(s) 22, 111
MwoI GCNNNNNNNGC 1 cut(s) 165
NarI GGCGCC 1 cut(s) 837
NdeI CATATG 2 cut(s) 934, 1124
NdeII GATC 4 cut(s) 171, 303, 727, 810
NlaIII CATG 5 cut(s) 172, 370, 395, 418, 811
NlaIV GGNNCC 2 cut(s) 313, 838
NmeAIII GCCGAG 1 cut(s) 813
NmuCI GTSAC 1 cut(s) 1058
PagI TCATGA 1 cut(s) 807
PciSI GCTCTTC 1 cut(s) 249
PcsI WCGNNNNNNNCGW 2 cut(s) 152, 968
PctI GAATGC 2 cut(s) 22, 111
PfeI GAWTC 2 cut(s) 485, 959
PkrI GCNGC 3 cut(s) 450, 912, 1032
PleI GAGTC 1 cut(s) 22
PluTI GGCGCC 1 cut(s) 840
PpsI GAGTC 1 cut(s) 22
Ppu21I YACGTR 1 cut(s) 15
Psp124BI GAGCTC 1 cut(s) 161
PspN4I GGNNCC 2 cut(s) 313, 838
PspPI GGNCC 3 cut(s) 38, 152, 312
PstI CTGCAG 1 cut(s) 450
PsuI RGATCY 1 cut(s) 727
RsaI GTAC 7 cut(s) 5, 195, 536, 743, 830, 859, 940
RsaNI GTAC 7 cut(s) 4, 194, 535, 742, 829, 858, 939
RseI CAYNNNNRTG 1 cut(s) 581
SacI GAGCTC 1 cut(s) 161
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 3 cut(s) 344, 420, 1168
SatI GCNGC 3 cut(s) 449, 911, 1031
Sau3AI GATC 4 cut(s) 171, 303, 727, 810
Sau96I GGNCC 3 cut(s) 38, 152, 312
ScaI AGTACT 1 cut(s) 859
SchI GAGTC 1 cut(s) 22
SduI GDGCHC 3 cut(s) 67, 161, 1087
SfaNI GCATC 1 cut(s) 150
SfcI CTRYAG 3 cut(s) 446, 861, 903
SfoI GGCGCC 1 cut(s) 838
SmiMI CAYNNNNRTG 1 cut(s) 581
SmlI CTYRAG 2 cut(s) 437, 874
SmoI CTYRAG 2 cut(s) 437, 874
Sse9I AATT 7 cut(s) 280, 472, 492, 509, 786, 885, 989
SsiI CCGC 3 cut(s) 46, 139, 911
SspDI GGCGCC 1 cut(s) 836
SspI AATATT 1 cut(s) 822
SspMI CTAG 1 cut(s) 180
SstI GAGCTC 1 cut(s) 161
TaaI ACNGT 5 cut(s) 583, 862, 922, 1064, 1082
TaiI ACGT 1 cut(s) 17
TaqI TCGA 5 cut(s) 26, 115, 641, 747, 971
TasI AATT 7 cut(s) 280, 472, 492, 509, 786, 885, 989
TatI WGTACW 3 cut(s) 3, 193, 857
TauI GCSGC 1 cut(s) 913
TfiI GAWTC 2 cut(s) 485, 959
Tru1I TTAA 3 cut(s) 344, 420, 1168
Tru9I TTAA 3 cut(s) 344, 420, 1168
TscAI CASTG 3 cut(s) 111, 588, 1067
TseFI GTSAC 1 cut(s) 1058
TseI GCWGC 2 cut(s) 448, 1030
Tsp45I GTSAC 1 cut(s) 1058
TspDTI ATGAA 2 cut(s) 498, 960
TspRI CASTG 3 cut(s) 111, 588, 1067
XapI RAATTY 3 cut(s) 472, 509, 786
XspI CTAG 1 cut(s) 180
ZrmI AGTACT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.