MD08G1089900.v1.1

Domain of unknown function (DUF4793)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002655
Physical Location & Seq
Reverse (-)
7432992 .. 7436771
3780 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1089900.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGTACCGTCCGGTGTGGGCCCACCCTACGCCTCCGGCAGGCTCTCAGAGGTGGCAGGAGACTTGGGCTCGCCTCCTCACTCCTCTGACCCTCTGGATATGTGTTTCGGTGAGTCTCCGATACGGGTACTATGGCGACCGCCGGATGGAGCTCGGGTCGAGCTCTTCGCGATTGATGAAGGCTAGTGCTGTGTTTGTCAAACATGTTGAAGTGAGAGATGTTGACAGGAAAGGGGTTTCTCTCTATGCTTTTTCTGAAGAGCCTAAATTGAACTACCAAACAAATTGGAGTGTGCACAATTACTTGATCATTCGATCGTATAATGGCAAGGGATTTTCCTTCTGGTTAAACAAGGGTTCAAGTTTGTCCATGAGATTGGAAGCTCATGCTACTACCTTAAATCAACTACAAGTTGCTATGCTTAAAGGGGAACAAAATATCGAAATATTGCAGCCGGAACAAACAAGTTCTCAGGACAGCCTCGCCTTCAATGAACCCAAAATCGGTAAAGAAGCCGAGTACAATATTGAGGAAGATGACAAGTATTACCTTGAATTCATAAGCACAAATCCTAATAGCGTAATTGTATCAGTGAATGTAAGCGTTTCATCAACGATGTATGATGTTACGAAAGCTAAGGACGTCTGTTCAACAACGAAGGGGTCCTGCCGGCTCAGCCTTGTATTCCCCAATACTCACTATGTCATACTAACCACACCTAACAATGGAGATCTTGGTGACTGGTACATCGAGCTTTCGTTCATGGCTCGTGTGGTAACCTACATTGCGATTTTAGGATTTATTGTGGGGGTTGTTTTTCTGATATTGAAATACCTTGGAGCCTGTGACGGTGATGATAGAACTTTAGTTGAAATAGCAGTGGAAGAAGTTAATGAGACTTACCCTATAGTGCCACAGAAGACGCTTCTTCGACAGACATACGGGACAAATGAAGAAGATGAAGATTCAGGAGCTTCTAGTAGCTCCTCGGAGGAATTATATGATGAGAAATTATGCGTAATTTGTTACGATGAGCAGCGCAATTCCTTCTTCGTTCCTTGTGGTCACTGTGCCACCTGCTATGACTGTGCTCAAAGGATCATGGATGGGGAGAATAAGGTGTGTCCAATATGTAGAAGGCTCATCCACAAAGTGAGAAAATTGTTCAATCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4792 DUF4793 zf-C3HC4_3

Protein Analysis

392

Amino Acids

44.39

Weight (kDa)

5.52

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APD1-4_N PF16040 79 - 146 2.4e-14 E3 ubiquitin-protein ligase APD1-4, N-terminal
APD1-4_M PF16041 172 - 274 3.3e-27 E3 ubiquitin-protein ligase APD1-4, middle domain
zf-C3HC4_3 PF13920 336 - 385 6.2e-16 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 338 - 379 6.4e-07 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1085
AatII GACGTC 1 cut(s) 645
Acc36I ACCTGC 1 cut(s) 1085
AccII CGCG 1 cut(s) 169
AciI CCGC 1 cut(s) 139
AclWI GGATC 1 cut(s) 1106
AcsI RAATTY 1 cut(s) 554
AcuI CTGAAG 1 cut(s) 276
AcyI GRCGYC 1 cut(s) 642
AdeI CACNNNGTG 1 cut(s) 1153
AfaI GTAC 4 cut(s) 5, 128, 521, 746
AfiI CCNNNNNNNGG 4 cut(s) 38, 145, 503, 725
AflIII ACRYGT 1 cut(s) 202
AgsI TTSAA 9 cut(s) 209, 271, 360, 490, 554, 651, 829, 872, 1168
AluBI AGCT 7 cut(s) 151, 162, 383, 635, 754, 974, 984
AluI AGCT 7 cut(s) 151, 162, 383, 635, 754, 974, 984
Alw21I GWGCWC 4 cut(s) 153, 164, 297, 1093
Alw26I GTCTC 3 cut(s) 53, 119, 890
Alw44I GTGCAC 1 cut(s) 293
AlwI GGATC 1 cut(s) 1106
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 1 cut(s) 18
ApaI GGGCCC 1 cut(s) 22
ApaLI GTGCAC 1 cut(s) 293
ApeKI GCWGC 2 cut(s) 451, 1036
ApoI RAATTY 1 cut(s) 554
AspLEI GCGC 1 cut(s) 1041
AspS9I GGNCC 3 cut(s) 18, 19, 663
AsuHPI GGTGA 3 cut(s) 121, 749, 863
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 1 cut(s) 663
BaeGI GKGCMC 2 cut(s) 22, 297
BanII GRGCYC 4 cut(s) 22, 70, 153, 164
BauI CACGAG 1 cut(s) 768
BbsI GAAGAC 1 cut(s) 926
Bbv12I GWGCWC 4 cut(s) 153, 164, 297, 1093
BbvI GCAGC 2 cut(s) 463, 1048
BccI CCATC 2 cut(s) 139, 1100
BclI TGATCA 1 cut(s) 306
BcoDI GTCTC 3 cut(s) 53, 119, 890
BfaI CTAG 2 cut(s) 183, 978
BfmI CTRYAG 1 cut(s) 906
BfuAI ACCTGC 1 cut(s) 1085
BglII AGATCT 1 cut(s) 730
BisI GCNGC 2 cut(s) 452, 1037
BlpI GCTNAGC 1 cut(s) 674
BlsI GCNGC 2 cut(s) 453, 1038
Bme18I GGWCC 1 cut(s) 663
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 3 cut(s) 18, 19, 663
BmiI GGNNCC 3 cut(s) 20, 664, 841
BpiI GAAGAC 1 cut(s) 926
Bpu10I CCTNAGC 1 cut(s) 636
Bpu1102I GCTNAGC 1 cut(s) 674
BsaHI GRCGYC 1 cut(s) 642
BsaJI CCNNGG 2 cut(s) 835, 987
BsaWI WCCGGW 1 cut(s) 10
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 4 cut(s) 38, 145, 503, 725
Bse118I RCCGGY 1 cut(s) 669
Bse1I ACTGG 1 cut(s) 746
Bse3DI GCAATG 1 cut(s) 783
BseDI CCNNGG 2 cut(s) 835, 987
BseGI GGATG 3 cut(s) 150, 1111, 1143
BseLI CCNNNNNNNGG 4 cut(s) 38, 145, 503, 725
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 3 cut(s) 59, 485, 688
BseNI ACTGG 1 cut(s) 746
BseRI GAGGAG 3 cut(s) 65, 72, 976
BseSI GKGCMC 2 cut(s) 22, 297
BseXI GCAGC 2 cut(s) 463, 1048
Bsh1236I CGCG 1 cut(s) 169
Bsh1285I CGRYCG 2 cut(s) 139, 317
BshFI GGCC 1 cut(s) 20
BsiEI CGRYCG 2 cut(s) 139, 317
BsiHKAI GWGCWC 4 cut(s) 153, 164, 297, 1093
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 5 cut(s) 11, 35, 142, 455, 670
BslFI GGGAC 1 cut(s) 958
BslI CCNNNNNNNGG 4 cut(s) 38, 145, 503, 725
BsmAI GTCTC 3 cut(s) 53, 119, 890
BsmFI GGGAC 1 cut(s) 958
BsnI GGCC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 152
Bsp120I GGGCCC 1 cut(s) 18
Bsp1286I GDGCHC 6 cut(s) 22, 70, 153, 164, 297, 1093
Bsp143I GATC 4 cut(s) 306, 314, 730, 1098
Bsp1720I GCTNAGC 1 cut(s) 674
Bsp68I TCGCGA 1 cut(s) 169
BspACI CCGC 1 cut(s) 139
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 3 cut(s) 58, 484, 687
BspFNI CGCG 1 cut(s) 169
BspLI GGNNCC 3 cut(s) 20, 664, 841
BspMI ACCTGC 1 cut(s) 1085
BspPI GGATC 1 cut(s) 1106
BspQI GCTCTTC 2 cut(s) 169, 252
BsrDI GCAATG 1 cut(s) 783
BsrFI RCCGGY 1 cut(s) 669
BsrI ACTGG 1 cut(s) 746
BssAI RCCGGY 1 cut(s) 669
BssECI CCNNGG 2 cut(s) 835, 987
BssMI GATC 4 cut(s) 306, 314, 730, 1098
BssNI GRCGYC 1 cut(s) 642
BssSI CACGAG 1 cut(s) 768
BssT1I CCWWGG 1 cut(s) 835
Bst2BI CACGAG 1 cut(s) 768
Bst4CI ACNGT 4 cut(s) 8, 851, 1070, 1088
Bst6I CTCTTC 2 cut(s) 169, 252
BstACI GRCGYC 1 cut(s) 642
BstC8I GCNNGC 3 cut(s) 40, 70, 671
BstDEI CTNAG 4 cut(s) 45, 471, 636, 674
BstEII GGTNACC 1 cut(s) 775
BstENI CCTNNNNNAGG 1 cut(s) 36
BstF5I GGATG 3 cut(s) 150, 1111, 1143
BstFNI CGCG 1 cut(s) 169
BstHHI GCGC 1 cut(s) 1041
BstKTI GATC 4 cut(s) 309, 317, 733, 1101
BstMAI GTCTC 3 cut(s) 53, 119, 890
BstMBI GATC 4 cut(s) 306, 314, 730, 1098
BstMCI CGRYCG 2 cut(s) 139, 317
BstMWI GCNNNNNNNGC 1 cut(s) 675
BstNSI RCATGY 1 cut(s) 206
BstPI GGTNACC 1 cut(s) 775
BstSFI CTRYAG 1 cut(s) 906
BstSLI GKGCMC 2 cut(s) 22, 297
BstUI CGCG 1 cut(s) 169
BstV1I GCAGC 2 cut(s) 463, 1048
BstV2I GAAGAC 1 cut(s) 926
BstX2I RGATCY 1 cut(s) 730
BstXI CCANNNNNNTGG 1 cut(s) 376
BstYI RGATCY 1 cut(s) 730
BsuRI GGCC 1 cut(s) 20
BtsCI GGATG 3 cut(s) 150, 1111, 1143
BtsI GCAGTG 1 cut(s) 885
BtsIMutI CAGTG 3 cut(s) 597, 885, 1066
BtuMI TCGCGA 1 cut(s) 169
BveI ACCTGC 1 cut(s) 1085
Cac8I GCNNGC 3 cut(s) 40, 70, 671
CfoI GCGC 1 cut(s) 1041
Cfr10I RCCGGY 1 cut(s) 669
Cfr13I GGNCC 3 cut(s) 18, 19, 663
CseI GACGC 1 cut(s) 931
Csp6I GTAC 4 cut(s) 4, 127, 520, 745
CviAII CATG 5 cut(s) 203, 370, 386, 763, 1102
CviQI GTAC 4 cut(s) 4, 127, 520, 745
DdeI CTNAG 4 cut(s) 45, 471, 636, 674
DpnI GATC 4 cut(s) 308, 316, 732, 1100
DpnII GATC 4 cut(s) 306, 314, 730, 1098
DraIII CACNNNGTG 1 cut(s) 1153
Eam1104I CTCTTC 2 cut(s) 169, 252
EarI CTCTTC 2 cut(s) 169, 252
Ecl136II GAGCTC 2 cut(s) 151, 162
Eco130I CCWWGG 1 cut(s) 835
Eco24I GRGCYC 4 cut(s) 22, 70, 153, 164
Eco47I GGWCC 1 cut(s) 663
Eco53kI GAGCTC 2 cut(s) 151, 162
Eco57I CTGAAG 1 cut(s) 276
Eco88I CYCGRG 1 cut(s) 152
Eco91I GGTNACC 1 cut(s) 775
EcoICRI GAGCTC 2 cut(s) 151, 162
EcoNI CCTNNNNNAGG 1 cut(s) 36
EcoO109I RGGNCCY 1 cut(s) 663
EcoO65I GGTNACC 1 cut(s) 775
EcoRI GAATTC 1 cut(s) 554
EcoT14I CCWWGG 1 cut(s) 835
EcoT38I GRGCYC 4 cut(s) 22, 70, 153, 164
ErhI CCWWGG 1 cut(s) 835
FaeI CATG 5 cut(s) 206, 373, 389, 766, 1105
FaqI GGGAC 1 cut(s) 958
FatI CATG 5 cut(s) 202, 369, 385, 762, 1101
FbaI TGATCA 1 cut(s) 306
Fnu4HI GCNGC 2 cut(s) 452, 1037
FokI GGATG 3 cut(s) 157, 1118, 1130
FriOI GRGCYC 4 cut(s) 22, 70, 153, 164
Fsp4HI GCNGC 2 cut(s) 452, 1037
FspBI CTAG 2 cut(s) 183, 978
GlaI GCGC 1 cut(s) 1040
GluI GCNGC 2 cut(s) 452, 1037
HaeIII GGCC 1 cut(s) 20
HapII CCGG 5 cut(s) 11, 35, 142, 455, 670
HgaI GACGC 1 cut(s) 931
HhaI GCGC 1 cut(s) 1041
Hin1I GRCGYC 1 cut(s) 642
Hin1II CATG 5 cut(s) 206, 373, 389, 766, 1105
Hin6I GCGC 1 cut(s) 1039
HinP1I GCGC 1 cut(s) 1039
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HinfI GANTC 2 cut(s) 112, 965
HpaII CCGG 5 cut(s) 11, 35, 142, 455, 670
HphI GGTGA 3 cut(s) 121, 749, 863
Hpy166II GTNNAC 2 cut(s) 223, 295
Hpy188I TCNGA 6 cut(s) 48, 87, 119, 256, 822, 991
Hpy188III TCNNGA 4 cut(s) 94, 168, 473, 969
Hpy8I GTNNAC 2 cut(s) 223, 295
HpyAV CCTTC 6 cut(s) 172, 349, 496, 652, 1057, 1131
HpyCH4III ACNGT 4 cut(s) 8, 851, 1070, 1088
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 2 cut(s) 295, 451
HpyF10VI GCNNNNNNNGC 1 cut(s) 675
HpyF3I CTNAG 4 cut(s) 45, 471, 636, 674
HpySE526I ACGT 1 cut(s) 642
Hsp92I GRCGYC 1 cut(s) 642
Hsp92II CATG 5 cut(s) 206, 373, 389, 766, 1105
HspAI GCGC 1 cut(s) 1039
KroI GCCGGC 1 cut(s) 669
KroNI GCCGGC 1 cut(s) 671
Ksp22I TGATCA 1 cut(s) 306
Kzo9I GATC 4 cut(s) 306, 314, 730, 1098
LguI GCTCTTC 2 cut(s) 169, 252
LmnI GCTCC 4 cut(s) 148, 839, 971, 989
Lsp1109I GCAGC 2 cut(s) 463, 1048
MaeI CTAG 2 cut(s) 183, 978
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 6 cut(s) 625, 737, 775, 845, 1025, 1064
MalI GATC 4 cut(s) 308, 316, 732, 1100
MboI GATC 4 cut(s) 306, 314, 730, 1098
MflI RGATCY 1 cut(s) 730
MhlI GDGCHC 6 cut(s) 22, 70, 153, 164, 297, 1093
MlyI GAGTC 1 cut(s) 121
MroNI GCCGGC 1 cut(s) 669
MseI TTAA 4 cut(s) 347, 398, 423, 891
MspI CCGG 5 cut(s) 11, 35, 142, 455, 670
MvnI CGCG 1 cut(s) 169
MwoI GCNNNNNNNGC 1 cut(s) 675
NaeI GCCGGC 1 cut(s) 671
NdeII GATC 4 cut(s) 306, 314, 730, 1098
NgoMIV GCCGGC 1 cut(s) 669
NlaIII CATG 5 cut(s) 206, 373, 389, 766, 1105
NlaIV GGNNCC 3 cut(s) 20, 664, 841
NmeAIII GCCGAG 1 cut(s) 541
NmuCI GTSAC 3 cut(s) 737, 845, 1064
NruI TCGCGA 1 cut(s) 169
NspI RCATGY 1 cut(s) 206
PaqCI CACCTGC 1 cut(s) 1085
PciI ACATGT 1 cut(s) 202
PciSI GCTCTTC 2 cut(s) 169, 252
PdiI GCCGGC 1 cut(s) 671
PfeI GAWTC 1 cut(s) 965
PkrI GCNGC 2 cut(s) 453, 1038
Ple19I CGATCG 1 cut(s) 317
PleI GAGTC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 120
PpuMI RGGWCCY 1 cut(s) 663
PscI ACATGT 1 cut(s) 202
Psp124BI GAGCTC 2 cut(s) 153, 164
Psp5II RGGWCCY 1 cut(s) 663
PspEI GGTNACC 1 cut(s) 775
PspN4I GGNNCC 3 cut(s) 20, 664, 841
PspOMI GGGCCC 1 cut(s) 18
PspPI GGNCC 3 cut(s) 18, 19, 663
PspPPI RGGWCCY 1 cut(s) 663
PsuI RGATCY 1 cut(s) 730
PvuI CGATCG 1 cut(s) 317
RruI TCGCGA 1 cut(s) 169
RsaI GTAC 4 cut(s) 5, 128, 521, 746
RsaNI GTAC 4 cut(s) 4, 127, 520, 745
SacI GAGCTC 2 cut(s) 153, 164
SapI GCTCTTC 2 cut(s) 169, 252
SaqAI TTAA 4 cut(s) 347, 398, 423, 891
SatI GCNGC 2 cut(s) 452, 1037
Sau3AI GATC 4 cut(s) 306, 314, 730, 1098
Sau96I GGNCC 3 cut(s) 18, 19, 663
SchI GAGTC 1 cut(s) 121
SduI GDGCHC 6 cut(s) 22, 70, 153, 164, 297, 1093
SfcI CTRYAG 1 cut(s) 906
SinI GGWCC 1 cut(s) 663
SsiI CCGC 1 cut(s) 139
SspI AATATT 2 cut(s) 447, 526
SspMI CTAG 2 cut(s) 183, 978
SstI GAGCTC 2 cut(s) 153, 164
StyI CCWWGG 1 cut(s) 835
TaaI ACNGT 4 cut(s) 8, 851, 1070, 1088
TaiI ACGT 1 cut(s) 645
TaqI TCGA 5 cut(s) 158, 313, 441, 750, 931
TatI WGTACW 1 cut(s) 519
TfiI GAWTC 1 cut(s) 965
Tru1I TTAA 4 cut(s) 347, 398, 423, 891
Tru9I TTAA 4 cut(s) 347, 398, 423, 891
TscAI CASTG 3 cut(s) 597, 885, 1073
TseFI GTSAC 3 cut(s) 737, 845, 1064
TseI GCWGC 2 cut(s) 451, 1036
Tsp45I GTSAC 3 cut(s) 737, 845, 1064
TspDTI ATGAA 7 cut(s) 191, 507, 547, 597, 751, 966, 975
TspRI CASTG 3 cut(s) 597, 885, 1073
VneI GTGCAC 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 663
XagI CCTNNNNNAGG 1 cut(s) 36
XapI RAATTY 1 cut(s) 554
XceI RCATGY 1 cut(s) 206
XspI CTAG 2 cut(s) 183, 978
ZraI GACGTC 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.