FvH4_2g33580

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005176
Physical Location & Seq
Forward (+)
25140205 .. 25142579
2375 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33580.t1

Sequence Viewer

Length: 1551 bp
ATGGGAGATCCCTTATTCTACAGTTTAGCTCTCATCGTCTTTTCTCTGCTTCTCATCAACCTTTTCATAACCAATAGATCACGTAAGTATAAGAATCTACCCCCAAGCCCACCTTGCCTTCCTGTCATCGGTCACCTTCATCTTCTCAGACAACCGATCCACCGAACCCTCCAATCTCTCTCCGCTAAATACGGCAAAATCCAACTACTCCGATGGGGTTCTCGGCGAGTTGTCTTGATCTCCACGCCGTTGATCGTTGAAGAGTGTTTCACTAAGCACGATATCGCCTTCTCCAACCGCCCGTTCTTGCTCGCCGGAAAACACTTCCACTACAACTACACAACCGTTGCCGTAGCTCCCTACGGCGACTTATGGCGCAACCTCCGCCGCATTATGTCCCTCGAGATCTTCTCCTCGTCACGCATGGCTCTGTTTTCAAGTATCACACGACAAGAAGTCCGGTTGTTACGCGATCAAATCTCCAGGAGTTGCAGTTCGGGTATGACAAAGGTTGAGCTTAAGTCCAAGTGTATCAATCTTCCGTTTAATGTGATGACGATGATGATCGTGGGGAAGCGGTATTACGGAGAAGATGTAGGAGAAGCTGAGGAAGCAAAGAATTTTCGAGAAGCTATTAGGGATGCAATGGAGTTAAACTCATCAGCAAATTTGGGGGACTTTTTGCCATTTTTACAGTGGGGTGACGTATCAGGGTTGGAGAAGAAGATGGTGAGGGTGATGGCTAAGATGGACAGTTTCTTGCAGGCTATGGTTTCGGATCGTCGTGAAACTATGTCATCGAATTGTGATCAAAACAAGGGTGGAGGAGAGGTCAGGAAGTTGCTGATTGATAACTTATTGTTGCTTCAGCTAACAGAACCCGAGCTCTACACTGATGAGATAATCAAAGGCATCATTCTGGCGTTGTTGGCGGCCGGCACTGAGACTGATTCAACGACATTAGAATGGGCCATGTCACTATTGCTGAACCATCCAGATCAGTTGGATCAAGTCAGAGCTGAGATTGACATCAAGATCGGACAAGGGCGTCTGTTGGATGAGCAAGACTTGCCAAATCTGAATCACTTACAAAATGTGATCAATGAGACACTGCGTTTGTACCCTCCTGTTCCACTGTTAGGGATTCGTGAAGCCTCGGCGGATTGTGTAGTAGGCGGTTTTGATGTGCCCTGTGGTACAATGATGGTGATCAACGCATGGGCTATTCATAGGGACCCTGAATTGTGGCATGAGCCAACACAATTTAAGCCAGAGAGATTTGAAGGATGGAGTGGTGAAGGTCCTCAAGGATATAGGCTAATTCCATTTGGTGGCGGAAGACGAGGTTGTCCCGGGGCTGGCCTAGCCAACCGGTTAATTGCACTGGTGTTAGGAAGCTTGATTCAGGCATTTGAGTGGGAGAGGATTAGTGAAGAGAAGGTGGACATGAGTGAAGGTTTAGGACTTACCATGCCCAAGATCAAGCCATTGGAAGCCTTGTGCAAACCACGCCCTTTGATGCAGAAGCTTCGAATTCAATTCGAAAATTAG

Protein Analysis

517

Amino Acids

58.32

Weight (kDa)

8.01

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 34 - 493 6.4e-93 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1047, 1347
AccB7I CCANNNNNTGG 1 cut(s) 1331
AccII CGCG 1 cut(s) 471
AciI CCGC 9 cut(s) 183, 298, 385, 388, 577, 932, 1160, 1176, 1335
AclWI GGATC 4 cut(s) 2, 151, 786, 1014
AcoI YGGCCR 1 cut(s) 933
AcsI RAATTY 3 cut(s) 619, 667, 1533
AcuI CTGAAG 1 cut(s) 851
AcyI GRCGYC 1 cut(s) 1048
AfaI GTAC 2 cut(s) 1121, 1198
AfiI CCNNNNNNNGG 4 cut(s) 128, 1139, 1331, 1358
AflII CTTAAG 1 cut(s) 518
AgeI ACCGGT 1 cut(s) 1371
AgsI TTSAA 5 cut(s) 260, 438, 954, 1283, 1538
AhdI GACNNNNNGTC 1 cut(s) 455
AjnI CCWGG 1 cut(s) 482
AjuI GAANNNNNNNTTGG 2 cut(s) 287, 319
AloI GAACNNNNNNTCC 2 cut(s) 478, 510
Alw21I GWGCWC 1 cut(s) 888
Alw26I GTCTC 2 cut(s) 938, 1100
AlwI GGATC 4 cut(s) 2, 151, 786, 1014
Ama87I CYCGRG 3 cut(s) 401, 881, 1352
AoxI GGCC 3 cut(s) 933, 969, 1360
ApoI RAATTY 3 cut(s) 619, 667, 1533
ArsI GACNNNNNNTTYG 2 cut(s) 1099, 1131
AsiGI ACCGGT 1 cut(s) 1371
AspLEI GCGC 1 cut(s) 378
AspS9I GGNCC 3 cut(s) 969, 1234, 1301
AsuC2I CCSGG 2 cut(s) 1353, 1354
AsuHPI GGTGA 6 cut(s) 125, 713, 742, 748, 1219, 1307
AsuII TTCGAA 2 cut(s) 1531, 1542
AvaI CYCGRG 3 cut(s) 401, 881, 1352
AvaII GGWCC 2 cut(s) 1234, 1301
BaeGI GKGCMC 1 cut(s) 1191
BanII GRGCYC 1 cut(s) 888
BarI GAAGNNNNNNTAC 2 cut(s) 566, 598
BbsI GAAGAC 1 cut(s) 1345
Bbv12I GWGCWC 1 cut(s) 888
BbvCI CCTCAGC 1 cut(s) 606
BccI CCATC 7 cut(s) 207, 721, 733, 742, 999, 1198, 1281
BceAI ACGGC 4 cut(s) 208, 232, 335, 379
BcgI CGANNNNNNTGC 2 cut(s) 1511, 1545
BciT130I CCWGG 1 cut(s) 484
BclI TGATCA 3 cut(s) 808, 1098, 1209
BcnI CCSGG 2 cut(s) 1353, 1354
BcoDI GTCTC 2 cut(s) 938, 1100
BfaI CTAG 1 cut(s) 1364
BfmI CTRYAG 1 cut(s) 19
BfrI CTTAAG 1 cut(s) 518
BglII AGATCT 1 cut(s) 405
BisI GCNGC 2 cut(s) 388, 933
BlsI GCNGC 2 cut(s) 389, 934
Bme1390I CCNGG 3 cut(s) 484, 1353, 1354
Bme18I GGWCC 2 cut(s) 1234, 1301
BmeRI GACNNNNNGTC 1 cut(s) 455
BmeT110I CYCGRG 3 cut(s) 401, 881, 1352
BmgT120I GGNCC 3 cut(s) 969, 1234, 1301
BmiI GGNNCC 2 cut(s) 1235, 1236
BmrFI CCNGG 3 cut(s) 484, 1353, 1354
BmsI GCATC 3 cut(s) 631, 921, 1509
BpiI GAAGAC 1 cut(s) 1345
BplI GAGNNNNNCTC 4 cut(s) 395, 427, 641, 673
BpmI CTGGAG 1 cut(s) 466
Bpu10I CCTNAGC 1 cut(s) 606
Bpu14I TTCGAA 2 cut(s) 1531, 1542
BpuEI CTTGAG 1 cut(s) 1290
BpuMI CCSGG 2 cut(s) 1353, 1354
BsaAI YACGTR 1 cut(s) 83
BsaBI GATNNNNATC 3 cut(s) 563, 1028, 1208
BsaHI GRCGYC 1 cut(s) 1048
BsaJI CCNNGG 3 cut(s) 1155, 1352, 1353
BsaWI WCCGGW 2 cut(s) 459, 1371
BsaXI ACNNNNNCTCC 6 cut(s) 478, 508, 579, 609, 816, 846
Bsc4I CCNNNNNNNGG 4 cut(s) 128, 1139, 1331, 1358
Bse118I RCCGGY 2 cut(s) 935, 1371
Bse1I ACTGG 1 cut(s) 1389
Bse3DI GCAATG 1 cut(s) 651
Bse8I GATNNNNATC 3 cut(s) 563, 1028, 1208
BseBI CCWGG 1 cut(s) 484
BseDI CCNNGG 3 cut(s) 1155, 1352, 1353
BseGI GGATG 4 cut(s) 646, 991, 1063, 1292
BseJI GATNNNNATC 3 cut(s) 563, 1028, 1208
BseLI CCNNNNNNNGG 4 cut(s) 128, 1139, 1331, 1358
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 4 cut(s) 160, 597, 933, 1011
BseNI ACTGG 1 cut(s) 1389
BseRI GAGGAG 2 cut(s) 403, 840
BseSI GKGCMC 1 cut(s) 1191
BseX3I CGGCCG 1 cut(s) 933
Bsh1236I CGCG 1 cut(s) 471
Bsh1285I CGRYCG 1 cut(s) 936
BshFI GGCC 3 cut(s) 935, 971, 1362
BshTI ACCGGT 1 cut(s) 1371
BsiEI CGRYCG 1 cut(s) 936
BsiHKAI GWGCWC 1 cut(s) 888
BsiHKCI CYCGRG 3 cut(s) 401, 881, 1352
BsiSI CCGG 5 cut(s) 315, 460, 936, 1353, 1372
BslFI GGGAC 4 cut(s) 382, 689, 1247, 1335
BslI CCNNNNNNNGG 4 cut(s) 128, 1139, 1331, 1358
BsmAI GTCTC 2 cut(s) 938, 1100
BsmFI GGGAC 4 cut(s) 382, 689, 1247, 1335
BsnI GGCC 3 cut(s) 935, 971, 1362
BsoBI CYCGRG 3 cut(s) 401, 881, 1352
Bsp119I TTCGAA 2 cut(s) 1531, 1542
Bsp1286I GDGCHC 2 cut(s) 888, 1191
BspACI CCGC 9 cut(s) 183, 298, 385, 388, 577, 932, 1160, 1176, 1335
BspANI GGCC 3 cut(s) 935, 971, 1362
BspCNI CTCAG 4 cut(s) 159, 598, 934, 1012
BspFNI CGCG 1 cut(s) 471
BspLI GGNNCC 2 cut(s) 1235, 1236
BspPI GGATC 4 cut(s) 2, 151, 786, 1014
BspT104I TTCGAA 2 cut(s) 1531, 1542
BspTI CTTAAG 1 cut(s) 518
BsrDI GCAATG 1 cut(s) 651
BsrFI RCCGGY 2 cut(s) 935, 1371
BsrI ACTGG 1 cut(s) 1389
BssAI RCCGGY 2 cut(s) 935, 1371
BssECI CCNNGG 3 cut(s) 1155, 1352, 1353
BssNI GRCGYC 1 cut(s) 1048
Bst2UI CCWGG 1 cut(s) 484
Bst4CI ACNGT 5 cut(s) 23, 346, 696, 755, 1137
Bst6I CTCTTC 2 cut(s) 255, 1428
BstACI GRCGYC 1 cut(s) 1048
BstAFI CTTAAG 1 cut(s) 518
BstAPI GCANNNNNTGC 1 cut(s) 1069
BstBAI YACGTR 1 cut(s) 83
BstBI TTCGAA 2 cut(s) 1531, 1542
BstC8I GCNNGC 4 cut(s) 312, 765, 937, 1360
BstDEI CTNAG 6 cut(s) 146, 273, 606, 744, 942, 1020
BstEII GGTNACC 1 cut(s) 131
BstF5I GGATG 4 cut(s) 646, 991, 1063, 1292
BstFNI CGCG 1 cut(s) 471
BstHHI GCGC 1 cut(s) 378
BstMAI GTCTC 2 cut(s) 938, 1100
BstMCI CGRYCG 1 cut(s) 936
BstMWI GCNNNNNNNGC 7 cut(s) 114, 384, 611, 929, 1069, 1364, 1509
BstNI CCWGG 1 cut(s) 484
BstPI GGTNACC 1 cut(s) 131
BstSCI CCNGG 3 cut(s) 482, 1351, 1352
BstSFI CTRYAG 1 cut(s) 19
BstSLI GKGCMC 1 cut(s) 1191
BstUI CGCG 1 cut(s) 471
BstV2I GAAGAC 1 cut(s) 1345
BstX2I RGATCY 2 cut(s) 7, 405
BstYI RGATCY 2 cut(s) 7, 405
BstZI CGGCCG 1 cut(s) 933
BsuRI GGCC 3 cut(s) 935, 971, 1362
BtsCI GGATG 4 cut(s) 646, 991, 1063, 1292
BtsI GCAGTG 1 cut(s) 1109
BtsIMutI CAGTG 6 cut(s) 701, 891, 939, 1109, 1133, 1382
Cac8I GCNNGC 4 cut(s) 312, 765, 937, 1360
CfoI GCGC 1 cut(s) 378
Cfr10I RCCGGY 2 cut(s) 935, 1371
Cfr13I GGNCC 3 cut(s) 969, 1234, 1301
Cfr9I CCCGGG 1 cut(s) 1352
CseI GACGC 1 cut(s) 1037
Csp6I GTAC 2 cut(s) 1120, 1197
CspAI ACCGGT 1 cut(s) 1371
CspCI CAANNNNNGTGG 2 cut(s) 1497, 1532
CviAII CATG 6 cut(s) 424, 973, 1218, 1250, 1447, 1471
CviQI GTAC 2 cut(s) 1120, 1197
DdeI CTNAG 6 cut(s) 146, 273, 606, 744, 942, 1020
DrdI GACNNNNNNGTC 2 cut(s) 1047, 1347
DriI GACNNNNNGTC 1 cut(s) 455
DseDI GACNNNNNNGTC 2 cut(s) 1047, 1347
EaeI YGGCCR 1 cut(s) 933
EagI CGGCCG 1 cut(s) 933
Eam1104I CTCTTC 2 cut(s) 255, 1428
Eam1105I GACNNNNNGTC 1 cut(s) 455
EarI CTCTTC 2 cut(s) 255, 1428
EciI GGCGGA 3 cut(s) 374, 1175, 1350
Ecl136II GAGCTC 1 cut(s) 886
EclXI CGGCCG 1 cut(s) 933
Eco24I GRGCYC 1 cut(s) 888
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 2 cut(s) 1234, 1301
Eco52I CGGCCG 1 cut(s) 933
Eco53kI GAGCTC 1 cut(s) 886
Eco57I CTGAAG 1 cut(s) 851
Eco88I CYCGRG 3 cut(s) 401, 881, 1352
Eco91I GGTNACC 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 886
EcoO109I RGGNCCY 2 cut(s) 1234, 1301
EcoO65I GGTNACC 1 cut(s) 131
EcoRI GAATTC 1 cut(s) 1533
EcoRII CCWGG 1 cut(s) 482
EcoRV GATATC 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 888
FaeI CATG 6 cut(s) 427, 976, 1221, 1253, 1450, 1474
FalI AAGNNNNNCTT 2 cut(s) 97, 129
FaqI GGGAC 4 cut(s) 382, 689, 1247, 1335
FatI CATG 6 cut(s) 423, 972, 1217, 1249, 1446, 1470
FbaI TGATCA 3 cut(s) 808, 1098, 1209
Fnu4HI GCNGC 2 cut(s) 388, 933
FokI GGATG 4 cut(s) 653, 978, 1070, 1299
FriOI GRGCYC 1 cut(s) 888
Fsp4HI GCNGC 2 cut(s) 388, 933
FspBI CTAG 1 cut(s) 1364
GlaI GCGC 1 cut(s) 377
GluI GCNGC 2 cut(s) 388, 933
GsuI CTGGAG 1 cut(s) 466
HaeIII GGCC 3 cut(s) 935, 971, 1362
HapII CCGG 5 cut(s) 315, 460, 936, 1353, 1372
HgaI GACGC 1 cut(s) 1037
HhaI GCGC 1 cut(s) 378
Hin1I GRCGYC 1 cut(s) 1048
Hin1II CATG 6 cut(s) 427, 976, 1221, 1253, 1450, 1474
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HindIII AAGCTT 2 cut(s) 1396, 1526
HinfI GANTC 5 cut(s) 94, 950, 1081, 1144, 1402
HpaII CCGG 5 cut(s) 315, 460, 936, 1353, 1372
HphI GGTGA 6 cut(s) 125, 713, 742, 748, 1219, 1307
Hpy166II GTNNAC 1 cut(s) 1444
Hpy188I TCNGA 6 cut(s) 149, 212, 778, 1016, 1040, 1080
Hpy188III TCNNGA 8 cut(s) 235, 403, 626, 785, 835, 995, 1033, 1148
Hpy8I GTNNAC 1 cut(s) 1444
Hpy99I CGWCG 1 cut(s) 786
HpyAV CCTTC 7 cut(s) 128, 146, 298, 1277, 1292, 1432, 1448
HpyCH4III ACNGT 5 cut(s) 23, 346, 696, 755, 1137
HpyCH4IV ACGT 2 cut(s) 82, 705
HpyCH4V TGCA 6 cut(s) 492, 644, 763, 1382, 1503, 1522
HpyF10VI GCNNNNNNNGC 7 cut(s) 114, 384, 611, 929, 1069, 1364, 1509
HpyF3I CTNAG 6 cut(s) 146, 273, 606, 744, 942, 1020
HpySE526I ACGT 2 cut(s) 82, 705
Hsp92I GRCGYC 1 cut(s) 1048
Hsp92II CATG 6 cut(s) 427, 976, 1221, 1253, 1450, 1474
HspAI GCGC 1 cut(s) 376
KflI GGGWCCC 1 cut(s) 1234
KroI GCCGGC 1 cut(s) 935
KroNI GCCGGC 1 cut(s) 937
Ksp22I TGATCA 3 cut(s) 808, 1098, 1209
LmnI GCTCC 1 cut(s) 361
LweI GCATC 3 cut(s) 631, 921, 1509
MaeI CTAG 1 cut(s) 1364
MaeII ACGT 2 cut(s) 82, 705
MaeIII GTNAC 5 cut(s) 131, 417, 465, 701, 975
MboII GAAGA 9 cut(s) 134, 272, 400, 530, 602, 733, 736, 1350, 1445
MflI RGATCY 2 cut(s) 7, 405
MhlI GDGCHC 2 cut(s) 888, 1191
MmeI TCCRAC 5 cut(s) 226, 318, 696, 984, 1035
MroNI GCCGGC 1 cut(s) 935
MseI TTAA 5 cut(s) 519, 546, 653, 1266, 1376
MslI CAYNNNNRTG 2 cut(s) 964, 1414
MspCI CTTAAG 1 cut(s) 518
MspI CCGG 5 cut(s) 315, 460, 936, 1353, 1372
MspR9I CCNGG 3 cut(s) 484, 1353, 1354
MvaI CCWGG 1 cut(s) 484
MvnI CGCG 1 cut(s) 471
MwoI GCNNNNNNNGC 7 cut(s) 114, 384, 611, 929, 1069, 1364, 1509
NaeI GCCGGC 1 cut(s) 937
NciI CCSGG 2 cut(s) 1353, 1354
NgoMIV GCCGGC 1 cut(s) 935
NlaIII CATG 6 cut(s) 427, 976, 1221, 1253, 1450, 1474
NlaIV GGNNCC 2 cut(s) 1235, 1236
NmeAIII GCCGAG 2 cut(s) 202, 1136
NmuCI GTSAC 4 cut(s) 131, 417, 701, 975
NspV TTCGAA 2 cut(s) 1531, 1542
PaeR7I CTCGAG 1 cut(s) 401
PdiI GCCGGC 1 cut(s) 937
PfeI GAWTC 5 cut(s) 94, 950, 1081, 1144, 1402
PflMI CCANNNNNTGG 1 cut(s) 1331
PfoI TCCNGGA 1 cut(s) 482
PinAI ACCGGT 1 cut(s) 1371
PkrI GCNGC 2 cut(s) 389, 934
Ppu21I YACGTR 1 cut(s) 83
PpuMI RGGWCCY 2 cut(s) 1234, 1301
Psp124BI GAGCTC 1 cut(s) 888
Psp5II RGGWCCY 2 cut(s) 1234, 1301
Psp6I CCWGG 1 cut(s) 482
PspEI GGTNACC 1 cut(s) 131
PspGI CCWGG 1 cut(s) 482
PspN4I GGNNCC 2 cut(s) 1235, 1236
PspPI GGNCC 3 cut(s) 969, 1234, 1301
PspPPI RGGWCCY 2 cut(s) 1234, 1301
PsuI RGATCY 2 cut(s) 7, 405
RsaI GTAC 2 cut(s) 1121, 1198
RsaNI GTAC 2 cut(s) 1120, 1197
RseI CAYNNNNRTG 2 cut(s) 964, 1414
SacI GAGCTC 1 cut(s) 888
SaqAI TTAA 5 cut(s) 519, 546, 653, 1266, 1376
SatI GCNGC 2 cut(s) 388, 933
Sau96I GGNCC 3 cut(s) 969, 1234, 1301
ScrFI CCNGG 3 cut(s) 484, 1353, 1354
SduI GDGCHC 2 cut(s) 888, 1191
SfaNI GCATC 3 cut(s) 631, 921, 1509
SfcI CTRYAG 1 cut(s) 19
Sfr274I CTCGAG 1 cut(s) 401
SfuI TTCGAA 2 cut(s) 1531, 1542
SinI GGWCC 2 cut(s) 1234, 1301
SlaI CTCGAG 1 cut(s) 401
SmaI CCCGGG 1 cut(s) 1354
SmiMI CAYNNNNRTG 2 cut(s) 964, 1414
SmlI CTYRAG 3 cut(s) 401, 518, 1305
SmoI CTYRAG 3 cut(s) 401, 518, 1305
SsiI CCGC 9 cut(s) 183, 298, 385, 388, 577, 932, 1160, 1176, 1335
SspMI CTAG 1 cut(s) 1364
SstI GAGCTC 1 cut(s) 888
StyD4I CCNGG 3 cut(s) 482, 1351, 1352
TaaI ACNGT 5 cut(s) 23, 346, 696, 755, 1137
TaiI ACGT 2 cut(s) 85, 708
TaqI TCGA 5 cut(s) 402, 625, 800, 1531, 1542
TaqII GACCGA 1 cut(s) 119
TauI GCSGC 2 cut(s) 390, 935
TfiI GAWTC 5 cut(s) 94, 950, 1081, 1144, 1402
Tru1I TTAA 5 cut(s) 519, 546, 653, 1266, 1376
Tru9I TTAA 5 cut(s) 519, 546, 653, 1266, 1376
TscAI CASTG 6 cut(s) 701, 898, 946, 1116, 1140, 1389
TseFI GTSAC 4 cut(s) 131, 417, 701, 975
Tsp45I GTSAC 4 cut(s) 131, 417, 701, 975
TspDTI ATGAA 3 cut(s) 55, 128, 1217
TspGWI ACGGA 2 cut(s) 531, 600
TspMI CCCGGG 1 cut(s) 1352
TspRI CASTG 6 cut(s) 701, 898, 946, 1116, 1140, 1389
Van91I CCANNNNNTGG 1 cut(s) 1331
Vha464I CTTAAG 1 cut(s) 518
VpaK11BI GGWCC 2 cut(s) 1234, 1301
XapI RAATTY 3 cut(s) 619, 667, 1533
XcmI CCANNNNNNNNNTGG 1 cut(s) 693
XhoI CTCGAG 1 cut(s) 401
XmaI CCCGGG 1 cut(s) 1352
XspI CTAG 1 cut(s) 1364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.