MD08G1091500.v1.1

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005176
Physical Location & Seq
Reverse (-)
7558871 .. 7561941
3071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1091500.v1.1.491

Sequence Viewer

Length: 1524 bp
ATGGAAGAACAATCACCCTTCTTCTGGTACACTACTACAGGTATCATCATCATCTTCCTCACCTTCCTCACCAAATTTAAACCAAAACACAAATTTACGAGTCTGCCCCCATCCCCGCCGTCTCTTCCAATCATCGGCCACCTTCACCTTCTAAAACATCCAGTCCACCGAACCCTCCACTCCCTTTCTTCAAAGTTGGGTAAAATCCTCCTCCTCAAATGGGGTTCGCGCGCAGTCCTCCTAGTCTCCTCCCCTTCCGCCGCTGAAGAATGCTACACCAAGCACGACGTTGCCTTCGCCAACCGTCCAAGCCTGCTCGCCGGCAAACACTTCCACTACAACTTCACAACCGTCGCGGCGGCATCCTACGGCGACCATTGGCGTTACCTCCGCCGCGTCATGACTCTGGAGTTCTTCTCCTCCTCCCGCCTTGCGGCGTTTGCAGCCGTCCGGCAAGGAGAAGTCCGTCTCTTACTGAACCAGATCATGAAGAAGTCAAAGGTCGAACTCAAGTCCAAATTTACGGAGCTTGCATTCAACGTGATGACGATGACGGTGGTGGGGAAGCGGTACTTCGGCGAAGACGTGGCAGACGATGAGGAGGCAAAGAATTTCCGGGAGGTTATGAGGGAGGCCGTGCACCTATCGGCGGCGACGAATTTGGGAGATTTTTTGCCGGTTTTGCAGTGGATGGATGCGACGGGTTTGGAGAAGAAGATGGTGAGGCTGATGAAAAAGATGGACGGCTTCTTGCAGAGTTTGATTGAGGAGCGCCGTGGGATTTTGGCGGCGGGTTTTGAAACGAACGGCGCAGAATTGAAGAAGTTGATGATCGACAACTTTTTGGCGTTGCAACAAACAGATCCCCAATTATATACCGATGAAATCGTCAAGGGAATCATTTTGGTGTTGCTGGTAGCTGGGACAGACACAACATCAACAACCCTAGAATGGGCAATGGCACTATTGCTAAACCATCCAGGTGCAATGGCGAAATTAAGAGCCGAGATAGATACCAAGACTGGATCTGATCATCGTCTGTTGGAAGAGCAAGACTTGCCAAACCTGAACTACTTGCAAAATGTGATCACCGAAACGTATCGTTTGTACCCTTCATTTCCAATTCTAGTGCCTCATGAAAACTCAGAGGACTGTGTGGTAGGGGGGTTCGACGTGCCGCATCACACAATGCTCGTGATCAATGCATGGGCTATCCACAGGAACCCTGAGATATGGGAGGACCCCGAAAAGTTTAAGCCGGAGAGGTTTGAAGGGTGGAGTGGTGAGGGGTCCGAAGGGTATAAGCTGATCCCATTTGGGGCTGGAAGGCGACGGTGTCCTGGGGCCAGCCTCGCGAACAAGTTGGTTGGGTTGGCGCTGGGAAGCTTGGTTCAGTCGTTTGAGTGGGAGAGGATTGGTGAAGAGGAGGTGGACATGAGTGAGGGTTTGGGGCTTACCATGCCAAGGGTCAAGCCCTTGGAGGCTATTTGCAAGCCACGCCCAATCATGCTATCTTCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

508

Amino Acids

57.17

Weight (kDa)

7.01

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 36 - 489 6.2e-99 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 229, 231, 356, 396, 1355
AclWI GGATC 3 cut(s) 857, 1033, 1303
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 5 cut(s) 74, 92, 518, 610, 658
AcuI CTGAAG 1 cut(s) 285
AfaI GTAC 3 cut(s) 29, 572, 1109
AfiI CCNNNNNNNGG 9 cut(s) 24, 134, 220, 433, 649, 951, 952, 1318, 1464
AgsI TTSAA 5 cut(s) 192, 538, 800, 820, 1271
AjiI CACGTC 2 cut(s) 586, 1174
AjnI CCWGG 2 cut(s) 979, 1339
AloI GAACNNNNNNTCC 2 cut(s) 1374, 1406
AluBI AGCT 4 cut(s) 529, 920, 1306, 1386
AluI AGCT 4 cut(s) 529, 920, 1306, 1386
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 3 cut(s) 126, 250, 473
Alw44I GTGCAC 1 cut(s) 638
AlwI GGATC 3 cut(s) 857, 1033, 1303
AoxI GGCC 3 cut(s) 136, 633, 1344
ApaLI GTGCAC 1 cut(s) 638
ApeKI GCWGC 1 cut(s) 443
ApoI RAATTY 5 cut(s) 74, 92, 518, 610, 658
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AspLEI GCGC 5 cut(s) 231, 233, 774, 812, 1378
AspS9I GGNCC 3 cut(s) 1240, 1290, 1344
AsuC2I CCSGG 1 cut(s) 617
AsuHPI GGTGA 8 cut(s) 6, 52, 61, 137, 733, 1081, 1295, 1430
AvaII GGWCC 2 cut(s) 1240, 1290
BaeGI GKGCMC 1 cut(s) 642
BauI CACGAG 1 cut(s) 1193
BbsI GAAGAC 1 cut(s) 588
Bbv12I GWGCWC 1 cut(s) 642
BbvI GCAGC 1 cut(s) 455
BccI CCATC 5 cut(s) 118, 685, 712, 733, 984
BceAI ACGGC 7 cut(s) 103, 385, 431, 620, 759, 760, 823
BciT130I CCWGG 2 cut(s) 981, 1341
BclI TGATCA 3 cut(s) 1030, 1086, 1197
BcnI CCSGG 1 cut(s) 617
BcoDI GTCTC 3 cut(s) 126, 250, 473
BfaI CTAG 3 cut(s) 242, 947, 1127
BfmI CTRYAG 1 cut(s) 36
BfoI RGCGCY 2 cut(s) 775, 1379
BisI GCNGC 9 cut(s) 261, 357, 360, 394, 435, 444, 651, 789, 1178
BlsI GCNGC 9 cut(s) 262, 358, 361, 395, 436, 445, 652, 790, 1179
Bme1390I CCNGG 3 cut(s) 617, 981, 1341
Bme18I GGWCC 2 cut(s) 1240, 1290
BmgBI CACGTC 2 cut(s) 586, 1174
BmgT120I GGNCC 3 cut(s) 1240, 1290, 1344
BmiI GGNNCC 4 cut(s) 1223, 1242, 1291, 1345
BmrFI CCNGG 3 cut(s) 617, 981, 1341
BmsI GCATC 3 cut(s) 371, 685, 1189
BpiI GAAGAC 1 cut(s) 588
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpmI CTGGAG 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 494
BpuMI CCSGG 1 cut(s) 617
BsaJI CCNNGG 4 cut(s) 775, 1340, 1463, 1476
Bsc4I CCNNNNNNNGG 9 cut(s) 24, 134, 220, 433, 649, 951, 952, 1318, 1464
Bse118I RCCGGY 2 cut(s) 320, 676
Bse1I ACTGG 2 cut(s) 161, 1027
Bse3DI GCAATG 2 cut(s) 963, 993
BseBI CCWGG 2 cut(s) 981, 1341
BseDI CCNNGG 4 cut(s) 775, 1340, 1463, 1476
BseGI GGATG 6 cut(s) 110, 157, 362, 696, 700, 976
BseLI CCNNNNNNNGG 9 cut(s) 24, 134, 220, 433, 649, 951, 952, 1318, 1464
BseMI GCAATG 2 cut(s) 963, 993
BseMII CTCAG 2 cut(s) 1158, 1218
BseNI ACTGG 2 cut(s) 161, 1027
BsePI GCGCGC 1 cut(s) 229
BseRI GAGGAG 8 cut(s) 200, 203, 238, 409, 412, 614, 782, 1439
BseSI GKGCMC 1 cut(s) 642
BseXI GCAGC 1 cut(s) 455
BseYI CCCAGC 2 cut(s) 920, 1378
Bsh1236I CGCG 5 cut(s) 229, 231, 356, 396, 1355
BshFI GGCC 3 cut(s) 138, 635, 1346
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 5 cut(s) 321, 451, 616, 677, 1259
BslFI GGGAC 1 cut(s) 937
BslI CCNNNNNNNGG 9 cut(s) 24, 134, 220, 433, 649, 951, 952, 1318, 1464
BsmAI GTCTC 3 cut(s) 126, 250, 473
BsmBI CGTCTC 2 cut(s) 126, 473
BsmFI GGGAC 1 cut(s) 937
BsmI GAATGC 2 cut(s) 275, 533
BsnI GGCC 3 cut(s) 138, 635, 1346
Bsp1286I GDGCHC 1 cut(s) 642
Bsp143I GATC 8 cut(s) 483, 831, 862, 1025, 1030, 1086, 1197, 1308
Bsp68I TCGCGA 1 cut(s) 1355
BspANI GGCC 3 cut(s) 138, 635, 1346
BspCNI CTCAG 2 cut(s) 1157, 1219
BspFNI CGCG 5 cut(s) 229, 231, 356, 396, 1355
BspHI TCATGA 3 cut(s) 399, 486, 1135
BspLI GGNNCC 4 cut(s) 1223, 1242, 1291, 1345
BspPI GGATC 3 cut(s) 857, 1033, 1303
BspQI GCTCTTC 1 cut(s) 1041
BsrDI GCAATG 2 cut(s) 963, 993
BsrFI RCCGGY 2 cut(s) 320, 676
BsrI ACTGG 2 cut(s) 161, 1027
BssAI RCCGGY 2 cut(s) 320, 676
BssECI CCNNGG 4 cut(s) 775, 1340, 1463, 1476
BssHII GCGCGC 1 cut(s) 229
BssMI GATC 8 cut(s) 483, 831, 862, 1025, 1030, 1086, 1197, 1308
BssSI CACGAG 1 cut(s) 1193
BssT1I CCWWGG 2 cut(s) 1463, 1476
Bst2BI CACGAG 1 cut(s) 1193
Bst2UI CCWGG 2 cut(s) 981, 1341
Bst4CI ACNGT 5 cut(s) 305, 352, 556, 1154, 1335
Bst6I CTCTTC 3 cut(s) 129, 1041, 1416
BstAPI GCANNNNNTGC 1 cut(s) 1057
BstC8I GCNNGC 7 cut(s) 231, 314, 318, 322, 531, 1348, 1493
BstDEI CTNAG 2 cut(s) 1144, 1227
BstDSI CCRYGG 1 cut(s) 775
BstF5I GGATG 6 cut(s) 110, 157, 362, 696, 700, 976
BstFNI CGCG 5 cut(s) 229, 231, 356, 396, 1355
BstH2I RGCGCY 2 cut(s) 775, 1379
BstHHI GCGC 5 cut(s) 231, 233, 774, 812, 1378
BstKTI GATC 8 cut(s) 486, 834, 865, 1028, 1033, 1089, 1200, 1311
BstMAI GTCTC 3 cut(s) 126, 250, 473
BstMBI GATC 8 cut(s) 483, 831, 862, 1025, 1030, 1086, 1197, 1308
BstMWI GCNNNNNNNGC 7 cut(s) 440, 443, 682, 1057, 1352, 1459, 1497
BstNI CCWGG 2 cut(s) 981, 1341
BstSCI CCNGG 3 cut(s) 615, 979, 1339
BstSFI CTRYAG 1 cut(s) 36
BstSLI GKGCMC 1 cut(s) 642
BstUI CGCG 5 cut(s) 229, 231, 356, 396, 1355
BstV1I GCAGC 1 cut(s) 455
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 2 cut(s) 862, 1025
BstYI RGATCY 2 cut(s) 862, 1025
BsuRI GGCC 3 cut(s) 138, 635, 1346
BtgI CCRYGG 1 cut(s) 775
BtrI CACGTC 2 cut(s) 586, 1174
BtsCI GGATG 6 cut(s) 110, 157, 362, 696, 700, 976
BtsI GCAGTG 1 cut(s) 692
BtsIMutI CAGTG 1 cut(s) 692
BtuMI TCGCGA 1 cut(s) 1355
Cac8I GCNNGC 7 cut(s) 231, 314, 318, 322, 531, 1348, 1493
CciI TCATGA 3 cut(s) 399, 486, 1135
CfoI GCGC 5 cut(s) 231, 233, 774, 812, 1378
Cfr10I RCCGGY 2 cut(s) 320, 676
Cfr13I GGNCC 3 cut(s) 1240, 1290, 1344
CseI GACGC 1 cut(s) 385
Csp6I GTAC 3 cut(s) 28, 571, 1108
CviAII CATG 8 cut(s) 400, 487, 1136, 1206, 1435, 1459, 1507, 1519
CviQI GTAC 3 cut(s) 28, 571, 1108
DdeI CTNAG 2 cut(s) 1144, 1227
DpnI GATC 8 cut(s) 485, 833, 864, 1027, 1032, 1088, 1199, 1310
DpnII GATC 8 cut(s) 483, 831, 862, 1025, 1030, 1086, 1197, 1308
DraI TTTAAA 1 cut(s) 79
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 3 cut(s) 129, 1041, 1416
EarI CTCTTC 3 cut(s) 129, 1041, 1416
EciI GGCGGA 2 cut(s) 247, 380
Eco130I CCWWGG 2 cut(s) 1463, 1476
Eco47I GGWCC 2 cut(s) 1240, 1290
Eco57I CTGAAG 1 cut(s) 285
EcoO109I RGGNCCY 1 cut(s) 1240
EcoRII CCWGG 2 cut(s) 979, 1339
EcoT14I CCWWGG 2 cut(s) 1463, 1476
EcoT22I ATGCAT 1 cut(s) 1207
ErhI CCWWGG 2 cut(s) 1463, 1476
Esp3I CGTCTC 2 cut(s) 126, 473
FaeI CATG 8 cut(s) 403, 490, 1139, 1209, 1438, 1462, 1510, 1522
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FaqI GGGAC 1 cut(s) 937
FatI CATG 8 cut(s) 399, 486, 1135, 1205, 1434, 1458, 1506, 1518
FauI CCCGC 3 cut(s) 123, 434, 784
FbaI TGATCA 3 cut(s) 1030, 1086, 1197
Fnu4HI GCNGC 9 cut(s) 261, 357, 360, 394, 435, 444, 651, 789, 1178
FokI GGATG 6 cut(s) 97, 144, 349, 703, 707, 963
Fsp4HI GCNGC 9 cut(s) 261, 357, 360, 394, 435, 444, 651, 789, 1178
FspBI CTAG 3 cut(s) 242, 947, 1127
GlaI GCGC 5 cut(s) 230, 232, 773, 811, 1377
GluI GCNGC 9 cut(s) 261, 357, 360, 394, 435, 444, 651, 789, 1178
GsaI CCCAGC 2 cut(s) 924, 1382
GsuI CTGGAG 1 cut(s) 428
HaeII RGCGCY 2 cut(s) 775, 1379
HaeIII GGCC 3 cut(s) 138, 635, 1346
HapII CCGG 5 cut(s) 321, 451, 616, 677, 1259
HgaI GACGC 1 cut(s) 385
HhaI GCGC 5 cut(s) 231, 233, 774, 812, 1378
Hin1II CATG 8 cut(s) 403, 490, 1139, 1209, 1438, 1462, 1510, 1522
Hin6I GCGC 5 cut(s) 229, 231, 772, 810, 1376
HinP1I GCGC 5 cut(s) 229, 231, 772, 810, 1376
HindIII AAGCTT 1 cut(s) 1384
HinfI GANTC 3 cut(s) 100, 403, 897
HpaII CCGG 5 cut(s) 321, 451, 616, 677, 1259
HphI GGTGA 8 cut(s) 6, 52, 61, 137, 733, 1081, 1295, 1430
Hpy166II GTNNAC 4 cut(s) 30, 166, 640, 1432
Hpy188I TCNGA 3 cut(s) 1030, 1147, 1294
Hpy188III TCNNGA 6 cut(s) 400, 407, 487, 1136, 1195, 1354
Hpy8I GTNNAC 4 cut(s) 30, 166, 640, 1432
Hpy99I CGWCG 6 cut(s) 290, 356, 658, 703, 1175, 1335
HpyCH4III ACNGT 5 cut(s) 305, 352, 556, 1154, 1335
HpyCH4IV ACGT 5 cut(s) 288, 540, 585, 1097, 1173
HpyF10VI GCNNNNNNNGC 7 cut(s) 440, 443, 682, 1057, 1352, 1459, 1497
HpyF3I CTNAG 2 cut(s) 1144, 1227
HpySE526I ACGT 5 cut(s) 288, 540, 585, 1097, 1173
Hsp92II CATG 8 cut(s) 403, 490, 1139, 1209, 1438, 1462, 1510, 1522
HspAI GCGC 5 cut(s) 229, 231, 772, 810, 1376
KroI GCCGGC 1 cut(s) 320
KroNI GCCGGC 1 cut(s) 322
Ksp22I TGATCA 3 cut(s) 1030, 1086, 1197
Kzo9I GATC 8 cut(s) 483, 831, 862, 1025, 1030, 1086, 1197, 1308
LguI GCTCTTC 1 cut(s) 1041
LmnI GCTCC 2 cut(s) 526, 769
Lsp1109I GCAGC 1 cut(s) 455
LweI GCATC 3 cut(s) 371, 685, 1189
MaeI CTAG 3 cut(s) 242, 947, 1127
MaeII ACGT 5 cut(s) 288, 540, 585, 1097, 1173
MaeIII GTNAC 1 cut(s) 383
MalI GATC 8 cut(s) 485, 833, 864, 1027, 1032, 1088, 1199, 1310
MboI GATC 8 cut(s) 483, 831, 862, 1025, 1030, 1086, 1197, 1308
MflI RGATCY 2 cut(s) 862, 1025
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 9 cut(s) 74, 92, 518, 610, 658, 815, 869, 995, 1122
MlyI GAGTC 2 cut(s) 109, 397
MmeI TCCRAC 1 cut(s) 1023
Mph1103I ATGCAT 1 cut(s) 1207
MroNI GCCGGC 1 cut(s) 320
MseI TTAA 3 cut(s) 78, 998, 1254
MslI CAYNNNNRTG 2 cut(s) 905, 981
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 5 cut(s) 321, 451, 616, 677, 1259
MspR9I CCNGG 3 cut(s) 617, 981, 1341
Mva1269I GAATGC 2 cut(s) 275, 533
MvaI CCWGG 2 cut(s) 981, 1341
MvnI CGCG 5 cut(s) 229, 231, 356, 396, 1355
MwoI GCNNNNNNNGC 7 cut(s) 440, 443, 682, 1057, 1352, 1459, 1497
NaeI GCCGGC 1 cut(s) 322
NciI CCSGG 1 cut(s) 617
NdeII GATC 8 cut(s) 483, 831, 862, 1025, 1030, 1086, 1197, 1308
NgoMIV GCCGGC 1 cut(s) 320
NlaIII CATG 8 cut(s) 403, 490, 1139, 1209, 1438, 1462, 1510, 1522
NlaIV GGNNCC 4 cut(s) 1223, 1242, 1291, 1345
NmeAIII GCCGAG 1 cut(s) 1030
NruI TCGCGA 1 cut(s) 1355
NsiI ATGCAT 1 cut(s) 1207
PagI TCATGA 3 cut(s) 399, 486, 1135
PauI GCGCGC 1 cut(s) 229
PciSI GCTCTTC 1 cut(s) 1041
PcsI WCGNNNNNNNCGW 3 cut(s) 582, 591, 653
PctI GAATGC 2 cut(s) 275, 533
PdiI GCCGGC 1 cut(s) 322
PfeI GAWTC 1 cut(s) 897
PflFI GACNNNGTC 1 cut(s) 1335
PfoI TCCNGGA 1 cut(s) 615
PkrI GCNGC 9 cut(s) 262, 358, 361, 395, 436, 445, 652, 790, 1179
PleI GAGTC 2 cut(s) 108, 397
PpsI GAGTC 2 cut(s) 108, 397
PpuMI RGGWCCY 1 cut(s) 1240
Psp5II RGGWCCY 1 cut(s) 1240
Psp6I CCWGG 2 cut(s) 979, 1339
PspFI CCCAGC 2 cut(s) 920, 1378
PspGI CCWGG 2 cut(s) 979, 1339
PspN4I GGNNCC 4 cut(s) 1223, 1242, 1291, 1345
PspPI GGNCC 3 cut(s) 1240, 1290, 1344
PspPPI RGGWCCY 1 cut(s) 1240
PsuI RGATCY 2 cut(s) 862, 1025
PsyI GACNNNGTC 1 cut(s) 1335
PteI GCGCGC 1 cut(s) 229
RruI TCGCGA 1 cut(s) 1355
RsaI GTAC 3 cut(s) 29, 572, 1109
RsaNI GTAC 3 cut(s) 28, 571, 1108
RseI CAYNNNNRTG 2 cut(s) 905, 981
SapI GCTCTTC 1 cut(s) 1041
SaqAI TTAA 3 cut(s) 78, 998, 1254
SatI GCNGC 9 cut(s) 261, 357, 360, 394, 435, 444, 651, 789, 1178
Sau3AI GATC 8 cut(s) 483, 831, 862, 1025, 1030, 1086, 1197, 1308
Sau96I GGNCC 3 cut(s) 1240, 1290, 1344
SchI GAGTC 2 cut(s) 109, 397
ScrFI CCNGG 3 cut(s) 617, 981, 1341
SduI GDGCHC 1 cut(s) 642
SfaNI GCATC 3 cut(s) 371, 685, 1189
SfcI CTRYAG 1 cut(s) 36
SinI GGWCC 2 cut(s) 1240, 1290
SmiMI CAYNNNNRTG 2 cut(s) 905, 981
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 9 cut(s) 74, 92, 518, 610, 658, 815, 869, 995, 1122
SspMI CTAG 3 cut(s) 242, 947, 1127
StyD4I CCNGG 3 cut(s) 615, 979, 1339
StyI CCWWGG 2 cut(s) 1463, 1476
TaaI ACNGT 5 cut(s) 305, 352, 556, 1154, 1335
TaiI ACGT 5 cut(s) 291, 543, 588, 1100, 1176
TaqI TCGA 3 cut(s) 504, 834, 1170
TasI AATT 9 cut(s) 74, 92, 518, 610, 658, 815, 869, 995, 1122
TauI GCSGC 8 cut(s) 263, 359, 362, 396, 437, 653, 791, 1180
TfiI GAWTC 1 cut(s) 897
Tru1I TTAA 3 cut(s) 78, 998, 1254
Tru9I TTAA 3 cut(s) 78, 998, 1254
TscAI CASTG 1 cut(s) 692
TseI GCWGC 1 cut(s) 443
TspDTI ATGAA 5 cut(s) 503, 746, 897, 1104, 1152
TspGWI ACGGA 2 cut(s) 455, 539
TspRI CASTG 1 cut(s) 692
Tth111I GACNNNGTC 1 cut(s) 1335
VneI GTGCAC 1 cut(s) 638
VpaK11BI GGWCC 2 cut(s) 1240, 1290
XapI RAATTY 5 cut(s) 74, 92, 518, 610, 658
XspI CTAG 3 cut(s) 242, 947, 1127
Zsp2I ATGCAT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.