FvH4_2g33630

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005176
Physical Location & Seq
Forward (+)
25167965 .. 25169976
2012 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33630.t1

Sequence Viewer

Length: 813 bp
ATGGCTAAGATGGACAGTTTCTTGCAGACTATGGTTTCGGATCGTCGTGAAACTTTGTCATCGAATTGTGATCAAAACAAGGGTGGAGGAGAGGTCAGGAAGTTGCTGATTGATAACTTATTGTTGCTTCAGCTAACAGAACCCGAGCTCTACACTGATGCGATCATCAAAGGCATCATTCTGGCGTTGTTGGCGGCCGGCACTGAGACTGATTCAACGACATTAGAATGGGCCATGTCACTATTGCTGAACCATCCAGATCAGTTGGATCAAGTCAGAGCTGAGATTGACATCAAGATCGGACAAGGGCGTCGGTTGGATGAGCAAGACTTGCCAAATCTGAATCACTTACAAAATGTGATCAATGAGACACTGCGTTTGTACCCTCCTGTTCCACTGTTAGGGGTTCGTGAAGCCTCGGCGGATTGTGTAGTAGGCGGTTTTGATGTGCCCTGTGGTACAATGATGGTGATCAACGCATGGGCTATTCATAGGGACCCTGAATTGTGGCATGAGCCAACACAATTTAAGCCAGAGAGATTTGAAGGATGGAGTGGTGAAGGTCCTCAAGGATATAGGCTAATTCCATTTGGTGGCGGAAGACGAGGTTGTCCCGGGGCTGGCCTAGCCAACCGGTTAATTGCACTGGTGTTAGGAAGCTTGATTCAGGCATTTGAGTGGGAGAGGATTAGTGAAGAGAAGGTGGACATGAGTGAAGGTTTAGGACTTACCATGCCCAAGATCAAGCCATTGGAAGCCTTGTGCAAACCACGCCCTTTGATGCAGAAGCTTCGAATTCAATTCGAAAATTAG

Protein Analysis

271

Amino Acids

30.12

Weight (kDa)

5.08

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 3 - 247 5.1e-65 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 309, 609
AccB7I CCANNNNNTGG 1 cut(s) 593
AciI CCGC 4 cut(s) 194, 422, 438, 597
AclWI GGATC 2 cut(s) 48, 276
AcoI YGGCCR 1 cut(s) 195
AcsI RAATTY 1 cut(s) 795
AcuI CTGAAG 1 cut(s) 113
AcyI GRCGYC 1 cut(s) 310
AfaI GTAC 2 cut(s) 383, 460
AfiI CCNNNNNNNGG 3 cut(s) 401, 593, 620
AgeI ACCGGT 1 cut(s) 633
AgsI TTSAA 3 cut(s) 216, 545, 800
AluBI AGCT 5 cut(s) 133, 148, 281, 660, 790
AluI AGCT 5 cut(s) 133, 148, 281, 660, 790
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 2 cut(s) 200, 362
AlwI GGATC 2 cut(s) 48, 276
Ama87I CYCGRG 2 cut(s) 143, 614
AoxI GGCC 3 cut(s) 195, 231, 622
ApoI RAATTY 1 cut(s) 795
ArsI GACNNNNNNTTYG 4 cut(s) 19, 51, 361, 393
AsiGI ACCGGT 1 cut(s) 633
AspS9I GGNCC 3 cut(s) 231, 496, 563
AsuC2I CCSGG 2 cut(s) 615, 616
AsuHPI GGTGA 2 cut(s) 481, 569
AsuII TTCGAA 2 cut(s) 793, 804
AvaI CYCGRG 2 cut(s) 143, 614
AvaII GGWCC 2 cut(s) 496, 563
BaeGI GKGCMC 1 cut(s) 453
BanII GRGCYC 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 607
Bbv12I GWGCWC 1 cut(s) 150
BccI CCATC 4 cut(s) 4, 261, 460, 543
BcgI CGANNNNNNTGC 2 cut(s) 773, 807
BclI TGATCA 3 cut(s) 70, 360, 471
BcnI CCSGG 2 cut(s) 615, 616
BcoDI GTCTC 2 cut(s) 200, 362
BfaI CTAG 1 cut(s) 626
BisI GCNGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 196
Bme1390I CCNGG 2 cut(s) 615, 616
Bme18I GGWCC 2 cut(s) 496, 563
BmeT110I CYCGRG 2 cut(s) 143, 614
BmgT120I GGNCC 3 cut(s) 231, 496, 563
BmiI GGNNCC 2 cut(s) 497, 498
BmrFI CCNGG 2 cut(s) 615, 616
BmsI GCATC 3 cut(s) 148, 183, 771
BpiI GAAGAC 1 cut(s) 607
Bpu14I TTCGAA 2 cut(s) 793, 804
BpuEI CTTGAG 1 cut(s) 552
BpuMI CCSGG 2 cut(s) 615, 616
BsaBI GATNNNNATC 2 cut(s) 290, 470
BsaHI GRCGYC 1 cut(s) 310
BsaJI CCNNGG 3 cut(s) 417, 614, 615
BsaWI WCCGGW 1 cut(s) 633
BsaXI ACNNNNNCTCC 2 cut(s) 78, 108
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 593, 620
Bse118I RCCGGY 2 cut(s) 197, 633
Bse1I ACTGG 1 cut(s) 651
Bse8I GATNNNNATC 2 cut(s) 290, 470
BseDI CCNNGG 3 cut(s) 417, 614, 615
BseGI GGATG 3 cut(s) 253, 325, 554
BseJI GATNNNNATC 2 cut(s) 290, 470
BseLI CCNNNNNNNGG 3 cut(s) 401, 593, 620
BseMII CTCAG 2 cut(s) 195, 273
BseNI ACTGG 1 cut(s) 651
BseRI GAGGAG 1 cut(s) 102
BseSI GKGCMC 1 cut(s) 453
BseX3I CGGCCG 1 cut(s) 195
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 3 cut(s) 197, 233, 624
BshTI ACCGGT 1 cut(s) 633
BsiEI CGRYCG 1 cut(s) 198
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 2 cut(s) 143, 614
BsiSI CCGG 3 cut(s) 198, 615, 634
BslFI GGGAC 2 cut(s) 509, 597
BslI CCNNNNNNNGG 3 cut(s) 401, 593, 620
BsmAI GTCTC 2 cut(s) 200, 362
BsmFI GGGAC 2 cut(s) 509, 597
BsnI GGCC 3 cut(s) 197, 233, 624
BsoBI CYCGRG 2 cut(s) 143, 614
Bsp119I TTCGAA 2 cut(s) 793, 804
Bsp1286I GDGCHC 2 cut(s) 150, 453
Bsp143I GATC 9 cut(s) 40, 70, 162, 259, 268, 297, 360, 471, 741
BspACI CCGC 4 cut(s) 194, 422, 438, 597
BspANI GGCC 3 cut(s) 197, 233, 624
BspCNI CTCAG 2 cut(s) 196, 274
BspLI GGNNCC 2 cut(s) 497, 498
BspPI GGATC 2 cut(s) 48, 276
BspT104I TTCGAA 2 cut(s) 793, 804
BsrFI RCCGGY 2 cut(s) 197, 633
BsrI ACTGG 1 cut(s) 651
BssAI RCCGGY 2 cut(s) 197, 633
BssECI CCNNGG 3 cut(s) 417, 614, 615
BssMI GATC 9 cut(s) 40, 70, 162, 259, 268, 297, 360, 471, 741
BssNI GRCGYC 1 cut(s) 310
Bst4CI ACNGT 2 cut(s) 17, 399
Bst6I CTCTTC 1 cut(s) 690
BstACI GRCGYC 1 cut(s) 310
BstAPI GCANNNNNTGC 1 cut(s) 331
BstBI TTCGAA 2 cut(s) 793, 804
BstC8I GCNNGC 2 cut(s) 199, 622
BstDEI CTNAG 3 cut(s) 6, 204, 282
BstF5I GGATG 3 cut(s) 253, 325, 554
BstKTI GATC 9 cut(s) 43, 73, 165, 262, 271, 300, 363, 474, 744
BstMAI GTCTC 2 cut(s) 200, 362
BstMBI GATC 9 cut(s) 40, 70, 162, 259, 268, 297, 360, 471, 741
BstMCI CGRYCG 1 cut(s) 198
BstMWI GCNNNNNNNGC 4 cut(s) 191, 331, 626, 771
BstSCI CCNGG 2 cut(s) 613, 614
BstSLI GKGCMC 1 cut(s) 453
BstV2I GAAGAC 1 cut(s) 607
BstZI CGGCCG 1 cut(s) 195
BsuRI GGCC 3 cut(s) 197, 233, 624
BtsCI GGATG 3 cut(s) 253, 325, 554
BtsI GCAGTG 1 cut(s) 371
BtsIMutI CAGTG 5 cut(s) 153, 201, 371, 395, 644
Cac8I GCNNGC 2 cut(s) 199, 622
Cfr10I RCCGGY 2 cut(s) 197, 633
Cfr13I GGNCC 3 cut(s) 231, 496, 563
Cfr9I CCCGGG 1 cut(s) 614
CseI GACGC 1 cut(s) 299
Csp6I GTAC 2 cut(s) 382, 459
CspAI ACCGGT 1 cut(s) 633
CspCI CAANNNNNGTGG 2 cut(s) 759, 794
CviAII CATG 5 cut(s) 235, 480, 512, 709, 733
CviQI GTAC 2 cut(s) 382, 459
DdeI CTNAG 3 cut(s) 6, 204, 282
DpnI GATC 9 cut(s) 42, 72, 164, 261, 270, 299, 362, 473, 743
DpnII GATC 9 cut(s) 40, 70, 162, 259, 268, 297, 360, 471, 741
DrdI GACNNNNNNGTC 2 cut(s) 309, 609
DseDI GACNNNNNNGTC 2 cut(s) 309, 609
EaeI YGGCCR 1 cut(s) 195
EagI CGGCCG 1 cut(s) 195
Eam1104I CTCTTC 1 cut(s) 690
EarI CTCTTC 1 cut(s) 690
EciI GGCGGA 2 cut(s) 437, 612
Ecl136II GAGCTC 1 cut(s) 148
EclXI CGGCCG 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 150
Eco47I GGWCC 2 cut(s) 496, 563
Eco52I CGGCCG 1 cut(s) 195
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 113
Eco88I CYCGRG 2 cut(s) 143, 614
EcoICRI GAGCTC 1 cut(s) 148
EcoO109I RGGNCCY 2 cut(s) 496, 563
EcoRI GAATTC 1 cut(s) 795
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 5 cut(s) 238, 483, 515, 712, 736
FaiI YATR 8 cut(s) 32, 236, 481, 492, 513, 576, 710, 734
FaqI GGGAC 2 cut(s) 509, 597
FatI CATG 5 cut(s) 234, 479, 511, 708, 732
FbaI TGATCA 3 cut(s) 70, 360, 471
Fnu4HI GCNGC 1 cut(s) 195
FokI GGATG 3 cut(s) 240, 332, 561
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 1 cut(s) 195
FspBI CTAG 1 cut(s) 626
GluI GCNGC 1 cut(s) 195
HaeIII GGCC 3 cut(s) 197, 233, 624
HapII CCGG 3 cut(s) 198, 615, 634
HgaI GACGC 1 cut(s) 299
Hin1I GRCGYC 1 cut(s) 310
Hin1II CATG 5 cut(s) 238, 483, 515, 712, 736
HindIII AAGCTT 2 cut(s) 658, 788
HinfI GANTC 3 cut(s) 212, 343, 664
HpaII CCGG 3 cut(s) 198, 615, 634
HphI GGTGA 2 cut(s) 481, 569
Hpy166II GTNNAC 1 cut(s) 706
Hpy188I TCNGA 4 cut(s) 40, 278, 302, 342
Hpy188III TCNNGA 5 cut(s) 47, 97, 257, 295, 410
Hpy8I GTNNAC 1 cut(s) 706
Hpy99I CGWCG 2 cut(s) 48, 315
HpyAV CCTTC 4 cut(s) 539, 554, 694, 710
HpyCH4III ACNGT 2 cut(s) 17, 399
HpyCH4V TGCA 4 cut(s) 25, 644, 765, 784
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 331, 626, 771
HpyF3I CTNAG 3 cut(s) 6, 204, 282
Hsp92I GRCGYC 1 cut(s) 310
Hsp92II CATG 5 cut(s) 238, 483, 515, 712, 736
KflI GGGWCCC 1 cut(s) 496
KroI GCCGGC 1 cut(s) 197
KroNI GCCGGC 1 cut(s) 199
Ksp22I TGATCA 3 cut(s) 70, 360, 471
Kzo9I GATC 9 cut(s) 40, 70, 162, 259, 268, 297, 360, 471, 741
LweI GCATC 3 cut(s) 148, 183, 771
MaeI CTAG 1 cut(s) 626
MaeIII GTNAC 1 cut(s) 237
MalI GATC 9 cut(s) 42, 72, 164, 261, 270, 299, 362, 473, 743
MboI GATC 9 cut(s) 40, 70, 162, 259, 268, 297, 360, 471, 741
MboII GAAGA 2 cut(s) 612, 707
MhlI GDGCHC 2 cut(s) 150, 453
MluCI AATT 8 cut(s) 64, 503, 524, 582, 639, 795, 800, 808
MmeI TCCRAC 2 cut(s) 246, 297
MnlI CCTC 7 cut(s) 80, 85, 396, 427, 576, 599, 678
MroNI GCCGGC 1 cut(s) 197
MseI TTAA 2 cut(s) 528, 638
MslI CAYNNNNRTG 2 cut(s) 226, 676
MspI CCGG 3 cut(s) 198, 615, 634
MspR9I CCNGG 2 cut(s) 615, 616
MwoI GCNNNNNNNGC 4 cut(s) 191, 331, 626, 771
NaeI GCCGGC 1 cut(s) 199
NciI CCSGG 2 cut(s) 615, 616
NdeII GATC 9 cut(s) 40, 70, 162, 259, 268, 297, 360, 471, 741
NgoMIV GCCGGC 1 cut(s) 197
NlaIII CATG 5 cut(s) 238, 483, 515, 712, 736
NlaIV GGNNCC 2 cut(s) 497, 498
NmeAIII GCCGAG 1 cut(s) 398
NmuCI GTSAC 1 cut(s) 237
NspV TTCGAA 2 cut(s) 793, 804
PdiI GCCGGC 1 cut(s) 199
PfeI GAWTC 3 cut(s) 212, 343, 664
PflMI CCANNNNNTGG 1 cut(s) 593
PinAI ACCGGT 1 cut(s) 633
PkrI GCNGC 1 cut(s) 196
PpuMI RGGWCCY 2 cut(s) 496, 563
Psp124BI GAGCTC 1 cut(s) 150
Psp5II RGGWCCY 2 cut(s) 496, 563
PspN4I GGNNCC 2 cut(s) 497, 498
PspPI GGNCC 3 cut(s) 231, 496, 563
PspPPI RGGWCCY 2 cut(s) 496, 563
RsaI GTAC 2 cut(s) 383, 460
RsaNI GTAC 2 cut(s) 382, 459
RseI CAYNNNNRTG 2 cut(s) 226, 676
SacI GAGCTC 1 cut(s) 150
SaqAI TTAA 2 cut(s) 528, 638
SatI GCNGC 1 cut(s) 195
Sau3AI GATC 9 cut(s) 40, 70, 162, 259, 268, 297, 360, 471, 741
Sau96I GGNCC 3 cut(s) 231, 496, 563
ScrFI CCNGG 2 cut(s) 615, 616
SduI GDGCHC 2 cut(s) 150, 453
SfaNI GCATC 3 cut(s) 148, 183, 771
SfuI TTCGAA 2 cut(s) 793, 804
SinI GGWCC 2 cut(s) 496, 563
SmaI CCCGGG 1 cut(s) 616
SmiMI CAYNNNNRTG 2 cut(s) 226, 676
SmlI CTYRAG 1 cut(s) 567
SmoI CTYRAG 1 cut(s) 567
Sse9I AATT 8 cut(s) 64, 503, 524, 582, 639, 795, 800, 808
SsiI CCGC 4 cut(s) 194, 422, 438, 597
SspMI CTAG 1 cut(s) 626
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 2 cut(s) 613, 614
TaaI ACNGT 2 cut(s) 17, 399
TaqI TCGA 3 cut(s) 62, 793, 804
TasI AATT 8 cut(s) 64, 503, 524, 582, 639, 795, 800, 808
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 3 cut(s) 212, 343, 664
Tru1I TTAA 2 cut(s) 528, 638
Tru9I TTAA 2 cut(s) 528, 638
TscAI CASTG 5 cut(s) 160, 208, 378, 402, 651
TseFI GTSAC 1 cut(s) 237
Tsp45I GTSAC 1 cut(s) 237
TspDTI ATGAA 1 cut(s) 479
TspMI CCCGGG 1 cut(s) 614
TspRI CASTG 5 cut(s) 160, 208, 378, 402, 651
Van91I CCANNNNNTGG 1 cut(s) 593
VpaK11BI GGWCC 2 cut(s) 496, 563
XapI RAATTY 1 cut(s) 795
XmaI CCCGGG 1 cut(s) 614
XspI CTAG 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.