FvH4_2g35100

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008052
Physical Location & Seq
Reverse (-)
25944685 .. 25948112
3428 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35100.t3

Sequence Viewer

Length: 1224 bp
ATGGCTTCGCTGGAATCGGAGGCCGGCTCCGTAGCTGTATCCTCCAATGGAATCAAAAACAGAGCGACAAAAAATAGGAAGAACAAGACCAAAAACGCTGCGTTTCGTTACCTCCCCAAATTTCGGTGCTTTAGTCTAAAATCCGAAACCGCTACTACTGATGCGAAGGGGAGTTTCGATATGGAGGCCGAGGATGACGAAACGGGTCGGAAAATGAACCCGACCCATCTTGTTATTATGGTGAACGGGCTTGTGGGCAGTGCCGAGGATTGGAGGTTTGCAGCGACCCAGTTTCTGAAGAGGTACCCGGAAGATGTTGTTGTGCATTGCAGTGAATGTAACTGTTCAATGTTGACGTTTGATGGTGTTGATGTGATGGGAGAGAGATTGGCAGAAGAGGTTAGATCTGTGATAAAACGGCATCGGAGTGTTGAGAAGATCTCGTTTGTTGGTCACTCGTTAGGGGGCCTTATAGCAAGGTATGCCATCGGCAGGCTATATGAACGTGGCACCACGAGGGAAGTTTCGCAGGAAAATGGTGAATGTAGGGGCGATGGAGTTGAGGATCCACAGTTGGAACAGAAAGCGAAAAGTAGAATTGCTGGACTGGAGCCGGTGAATTTTATAACATCTGCAACGCCGCACCTAGGTACATGGGGGCATAAACAGGTTCCAGTGTTTTGTGGCTTTCAAATCCTTGAAAAAACAGCAGCTCGGATTTCATGGTGTCTAGGTAGAACTGGTAGACATCTTTTTTTAACTGATAGGGATAAAGGAAAACCTCCCCTTTTACTTCAGATGGTTTATGATACTGAAGATATTAAATTCTTATCTGCATTGAAGTCCTTCAGGCGTCATGTTGCCTATGCAAATGTCCATTATGACCACCTCGTGGGATGGAGTACATCTTCTATCCGTCGTAAGAAGGAGCTGCCGAAGTCTAAGCATTTATCCAGAGACGACAAATATCCACATATTGTACATGAAATGATAGACACTGCTGGTACTCAAGAAGAAGTTTCTGCAGAGGATGATGTGAATATTGACTTTGAAGAGGAAATGATCAGAGGTTTGACCAAAGTGAGCTGGGAAAGGATTGATGTGAGTTTTAGTGGGAGTAGACAAAAATACCTTGCGCACAGTACCATTCAGGTGAAAACTTATTGTATACATTCTGATGGAGCTGATGTGATTCAACACATGGTGGACAATTTTCATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

408

Amino Acids

45.93

Weight (kDa)

7.67

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 73 - 302 1.6e-65 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 626
Acc16I TGCGCA 1 cut(s) 1137
Acc65I GGTACC 1 cut(s) 303
AccB1I GGYRCC 2 cut(s) 303, 509
AccB7I CCANNNNNTGG 1 cut(s) 892
AccI GTMKAC 3 cut(s) 745, 1120, 1168
AciI CCGC 2 cut(s) 150, 641
AclWI GGATC 2 cut(s) 560, 573
AcsI RAATTY 3 cut(s) 119, 619, 824
AcuI CTGAAG 4 cut(s) 317, 779, 832, 834
AcyI GRCGYC 1 cut(s) 853
AdeI CACNNNGTG 2 cut(s) 892, 1204
AfaI GTAC 6 cut(s) 305, 652, 904, 981, 1006, 1144
AfiI CCNNNNNNNGG 5 cut(s) 123, 270, 492, 647, 892
AgsI TTSAA 6 cut(s) 348, 692, 701, 841, 1052, 1196
AluBI AGCT 5 cut(s) 35, 713, 931, 1086, 1184
AluI AGCT 5 cut(s) 35, 713, 931, 1086, 1184
Alw26I GTCTC 1 cut(s) 951
AlwI GGATC 2 cut(s) 560, 573
AlwNI CAGNNNCTG 1 cut(s) 295
AoxI GGCC 3 cut(s) 21, 186, 466
ApeKI GCWGC 4 cut(s) 98, 281, 710, 931
ApoI RAATTY 3 cut(s) 119, 619, 824
Asp700I GAANNNNTTC 1 cut(s) 845
Asp718I GGTACC 1 cut(s) 303
AspA2I CCTAGG 1 cut(s) 646
AspLEI GCGC 1 cut(s) 1138
AspS9I GGNCC 1 cut(s) 466
AsuC2I CCSGG 1 cut(s) 308
AsuHPI GGTGA 4 cut(s) 253, 551, 628, 1165
AvrII CCTAGG 1 cut(s) 646
BamHI GGATCC 1 cut(s) 565
BanI GGYRCC 2 cut(s) 303, 509
BauI CACGAG 2 cut(s) 514, 890
BbvI GCAGC 4 cut(s) 85, 293, 722, 918
BccI CCATC 8 cut(s) 234, 356, 370, 494, 548, 793, 891, 1172
BceAI ACGGC 1 cut(s) 434
BciVI GTATCC 1 cut(s) 49
BclI TGATCA 1 cut(s) 1062
BcnI CCSGG 1 cut(s) 308
BcoDI GTCTC 1 cut(s) 951
BfaI CTAG 2 cut(s) 647, 731
BfmI CTRYAG 1 cut(s) 1023
BfuI GTATCC 1 cut(s) 49
BglII AGATCT 2 cut(s) 404, 438
BisI GCNGC 5 cut(s) 99, 282, 641, 711, 932
BlnI CCTAGG 1 cut(s) 646
BlsI GCNGC 5 cut(s) 100, 283, 642, 712, 933
Bme1390I CCNGG 1 cut(s) 308
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 7 cut(s) 28, 305, 467, 511, 567, 612, 672
BmrFI CCNGG 1 cut(s) 308
BmrI ACTGGG 1 cut(s) 283
BmsI GCATC 2 cut(s) 151, 430
BmuI ACTGGG 1 cut(s) 283
BplI GAGNNNNNCTC 4 cut(s) 11, 43, 425, 457
BpmI CTGGAG 1 cut(s) 629
BpuEI CTTGAG 1 cut(s) 993
BpuMI CCSGG 1 cut(s) 308
BsaHI GRCGYC 1 cut(s) 853
BsaJI CCNNGG 3 cut(s) 189, 264, 646
BsaXI ACNNNNNCTCC 2 cut(s) 1173, 1203
Bsc4I CCNNNNNNNGG 5 cut(s) 123, 270, 492, 647, 892
Bse118I RCCGGY 2 cut(s) 23, 613
Bse1I ACTGG 4 cut(s) 289, 612, 674, 745
Bse3DI GCAATG 1 cut(s) 325
BseDI CCNNGG 3 cut(s) 189, 264, 646
BseGI GGATG 3 cut(s) 199, 902, 1036
BseLI CCNNNNNNNGG 5 cut(s) 123, 270, 492, 647, 892
BseMI GCAATG 1 cut(s) 325
BseNI ACTGG 4 cut(s) 289, 612, 674, 745
BseXI GCAGC 4 cut(s) 85, 293, 722, 918
BseYI CCCAGC 1 cut(s) 1086
BshFI GGCC 3 cut(s) 23, 188, 468
BshNI GGYRCC 2 cut(s) 303, 509
BsiSI CCGG 3 cut(s) 24, 308, 614
BslI CCNNNNNNNGG 5 cut(s) 123, 270, 492, 647, 892
BsmAI GTCTC 1 cut(s) 951
BsmBI CGTCTC 1 cut(s) 951
BsnI GGCC 3 cut(s) 23, 188, 468
Bsp1407I TGTACA 1 cut(s) 979
Bsp143I GATC 4 cut(s) 404, 438, 565, 1062
BspACI CCGC 2 cut(s) 150, 641
BspANI GGCC 3 cut(s) 23, 188, 468
BspLI GGNNCC 7 cut(s) 28, 305, 467, 511, 567, 612, 672
BspMAI CTGCAG 1 cut(s) 1027
BspPI GGATC 2 cut(s) 560, 573
BspT107I GGYRCC 2 cut(s) 303, 509
BsrDI GCAATG 1 cut(s) 325
BsrFI RCCGGY 2 cut(s) 23, 613
BsrGI TGTACA 1 cut(s) 979
BsrI ACTGG 4 cut(s) 289, 612, 674, 745
BssAI RCCGGY 2 cut(s) 23, 613
BssECI CCNNGG 3 cut(s) 189, 264, 646
BssMI GATC 4 cut(s) 404, 438, 565, 1062
BssNAI GTATAC 1 cut(s) 1169
BssNI GRCGYC 1 cut(s) 853
BssSI CACGAG 2 cut(s) 514, 890
BssT1I CCWWGG 1 cut(s) 646
Bst1107I GTATAC 1 cut(s) 1169
Bst2BI CACGAG 2 cut(s) 514, 890
Bst4CI ACNGT 3 cut(s) 344, 573, 1142
Bst6I CTCTTC 3 cut(s) 293, 390, 1047
BstACI GRCGYC 1 cut(s) 853
BstAPI GCANNNNNTGC 1 cut(s) 482
BstAUI TGTACA 1 cut(s) 979
BstC8I GCNNGC 2 cut(s) 25, 494
BstDEI CTNAG 1 cut(s) 942
BstF5I GGATG 3 cut(s) 199, 902, 1036
BstHHI GCGC 1 cut(s) 1138
BstKTI GATC 4 cut(s) 407, 441, 568, 1065
BstMAI GTCTC 1 cut(s) 951
BstMBI GATC 4 cut(s) 404, 438, 565, 1062
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 306
BstSFI CTRYAG 1 cut(s) 1023
BstV1I GCAGC 4 cut(s) 85, 293, 722, 918
BstX2I RGATCY 3 cut(s) 404, 438, 565
BstYI RGATCY 3 cut(s) 404, 438, 565
BstZ17I GTATAC 1 cut(s) 1169
BsuI GTATCC 1 cut(s) 49
BsuRI GGCC 3 cut(s) 23, 188, 468
BtgZI GCGATG 1 cut(s) 567
BtsCI GGATG 3 cut(s) 199, 902, 1036
BtsI GCAGTG 3 cut(s) 265, 337, 996
BtsIMutI CAGTG 4 cut(s) 265, 337, 681, 996
Cac8I GCNNGC 2 cut(s) 25, 494
CaiI CAGNNNCTG 1 cut(s) 295
CfoI GCGC 1 cut(s) 1138
Cfr10I RCCGGY 2 cut(s) 23, 613
Cfr13I GGNCC 1 cut(s) 466
CseI GACGC 1 cut(s) 842
Csp6I GTAC 6 cut(s) 304, 651, 903, 980, 1005, 1143
CviAII CATG 6 cut(s) 654, 723, 857, 983, 1201, 1217
CviQI GTAC 6 cut(s) 304, 651, 903, 980, 1005, 1143
DdeI CTNAG 1 cut(s) 942
DpnI GATC 4 cut(s) 406, 440, 567, 1064
DpnII GATC 4 cut(s) 404, 438, 565, 1062
DraIII CACNNNGTG 2 cut(s) 892, 1204
Eam1104I CTCTTC 3 cut(s) 293, 390, 1047
EarI CTCTTC 3 cut(s) 293, 390, 1047
Eco130I CCWWGG 1 cut(s) 646
Eco57I CTGAAG 4 cut(s) 317, 779, 832, 834
EcoO109I RGGNCCY 1 cut(s) 466
EcoT14I CCWWGG 1 cut(s) 646
ErhI CCWWGG 1 cut(s) 646
Esp3I CGTCTC 1 cut(s) 951
FaeI CATG 6 cut(s) 657, 726, 860, 986, 1204, 1220
FatI CATG 6 cut(s) 653, 722, 856, 982, 1200, 1216
FbaI TGATCA 1 cut(s) 1062
FblI GTMKAC 3 cut(s) 745, 1120, 1168
Fnu4HI GCNGC 5 cut(s) 99, 282, 641, 711, 932
FokI GGATG 3 cut(s) 206, 909, 1043
Fsp4HI GCNGC 5 cut(s) 99, 282, 641, 711, 932
FspBI CTAG 2 cut(s) 647, 731
FspI TGCGCA 1 cut(s) 1137
GlaI GCGC 1 cut(s) 1137
GluI GCNGC 5 cut(s) 99, 282, 641, 711, 932
GsaI CCCAGC 1 cut(s) 1090
GsuI CTGGAG 1 cut(s) 629
HaeIII GGCC 3 cut(s) 23, 188, 468
HapII CCGG 3 cut(s) 24, 308, 614
HgaI GACGC 1 cut(s) 842
HhaI GCGC 1 cut(s) 1138
Hin1I GRCGYC 1 cut(s) 853
Hin1II CATG 6 cut(s) 657, 726, 860, 986, 1204, 1220
Hin6I GCGC 1 cut(s) 1136
HinP1I GCGC 1 cut(s) 1136
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HinfI GANTC 3 cut(s) 14, 51, 1192
HpaII CCGG 3 cut(s) 24, 308, 614
HphI GGTGA 4 cut(s) 253, 551, 628, 1165
Hpy166II GTNNAC 6 cut(s) 244, 354, 746, 1121, 1169, 1207
Hpy188I TCNGA 9 cut(s) 19, 145, 210, 297, 426, 717, 798, 1067, 1177
Hpy188III TCNNGA 2 cut(s) 954, 1010
Hpy8I GTNNAC 6 cut(s) 244, 354, 746, 1121, 1169, 1207
Hpy99I CGWCG 1 cut(s) 921
HpyAV CCTTC 3 cut(s) 160, 856, 919
HpyCH4III ACNGT 3 cut(s) 344, 573, 1142
HpyCH4IV ACGT 2 cut(s) 356, 505
HpyCH4V TGCA 7 cut(s) 281, 325, 330, 635, 836, 869, 1025
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 1 cut(s) 942
HpySE526I ACGT 2 cut(s) 356, 505
Hsp92I GRCGYC 1 cut(s) 853
Hsp92II CATG 6 cut(s) 657, 726, 860, 986, 1204, 1220
HspAI GCGC 1 cut(s) 1136
KpnI GGTACC 1 cut(s) 307
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 1062
Kzo9I GATC 4 cut(s) 404, 438, 565, 1062
LmnI GCTCC 4 cut(s) 32, 610, 928, 1181
Lsp1109I GCAGC 4 cut(s) 85, 293, 722, 918
LweI GCATC 2 cut(s) 151, 430
MaeI CTAG 2 cut(s) 647, 731
MaeII ACGT 2 cut(s) 356, 505
MaeIII GTNAC 3 cut(s) 107, 338, 452
MalI GATC 4 cut(s) 406, 440, 567, 1064
MboI GATC 4 cut(s) 404, 438, 565, 1062
MboII GAAGA 9 cut(s) 91, 310, 323, 407, 448, 827, 900, 1025, 1064
MflI RGATCY 3 cut(s) 404, 438, 565
MluCI AATT 5 cut(s) 119, 597, 619, 824, 1210
MmeI TCCRAC 2 cut(s) 188, 555
MroNI GCCGGC 1 cut(s) 23
MroXI GAANNNNTTC 1 cut(s) 845
MseI TTAA 2 cut(s) 758, 822
MslI CAYNNNNRTG 4 cut(s) 330, 426, 1151, 1176
MspI CCGG 3 cut(s) 24, 308, 614
MspR9I CCNGG 1 cut(s) 308
MwoI GCNNNNNNNGC 1 cut(s) 482
NaeI GCCGGC 1 cut(s) 25
NciI CCSGG 1 cut(s) 308
NdeII GATC 4 cut(s) 404, 438, 565, 1062
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 6 cut(s) 657, 726, 860, 986, 1204, 1220
NlaIV GGNNCC 7 cut(s) 28, 305, 467, 511, 567, 612, 672
NmeAIII GCCGAG 2 cut(s) 214, 289
NmuCI GTSAC 1 cut(s) 452
NsbI TGCGCA 1 cut(s) 1137
PdiI GCCGGC 1 cut(s) 25
PdmI GAANNNNTTC 1 cut(s) 845
PfeI GAWTC 3 cut(s) 14, 51, 1192
PflMI CCANNNNNTGG 1 cut(s) 892
PkrI GCNGC 5 cut(s) 100, 283, 642, 712, 933
PsiI TTATAA 1 cut(s) 626
PspFI CCCAGC 1 cut(s) 1086
PspN4I GGNNCC 7 cut(s) 28, 305, 467, 511, 567, 612, 672
PspPI GGNCC 1 cut(s) 466
PstI CTGCAG 1 cut(s) 1027
PstNI CAGNNNCTG 1 cut(s) 295
PsuI RGATCY 3 cut(s) 404, 438, 565
RsaI GTAC 6 cut(s) 305, 652, 904, 981, 1006, 1144
RsaNI GTAC 6 cut(s) 304, 651, 903, 980, 1005, 1143
RseI CAYNNNNRTG 4 cut(s) 330, 426, 1151, 1176
SaqAI TTAA 2 cut(s) 758, 822
SatI GCNGC 5 cut(s) 99, 282, 641, 711, 932
Sau3AI GATC 4 cut(s) 404, 438, 565, 1062
Sau96I GGNCC 1 cut(s) 466
ScrFI CCNGG 1 cut(s) 308
SfaNI GCATC 2 cut(s) 151, 430
SfcI CTRYAG 1 cut(s) 1023
SmiMI CAYNNNNRTG 4 cut(s) 330, 426, 1151, 1176
SmlI CTYRAG 1 cut(s) 1008
SmoI CTYRAG 1 cut(s) 1008
Sse9I AATT 5 cut(s) 119, 597, 619, 824, 1210
SsiI CCGC 2 cut(s) 150, 641
SspI AATATT 1 cut(s) 1042
SspMI CTAG 2 cut(s) 647, 731
StyD4I CCNGG 1 cut(s) 306
StyI CCWWGG 1 cut(s) 646
TaaI ACNGT 3 cut(s) 344, 573, 1142
TaiI ACGT 2 cut(s) 359, 508
TaqI TCGA 1 cut(s) 177
TasI AATT 5 cut(s) 119, 597, 619, 824, 1210
TatI WGTACW 2 cut(s) 902, 979
TauI GCSGC 1 cut(s) 643
TfiI GAWTC 3 cut(s) 14, 51, 1192
Tru1I TTAA 2 cut(s) 758, 822
Tru9I TTAA 2 cut(s) 758, 822
TscAI CASTG 4 cut(s) 265, 337, 681, 1003
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 4 cut(s) 98, 281, 710, 931
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 5 cut(s) 230, 516, 711, 999, 1205
TspGWI ACGGA 2 cut(s) 19, 905
TspRI CASTG 4 cut(s) 265, 337, 681, 1003
Van91I CCANNNNNTGG 1 cut(s) 892
XapI RAATTY 3 cut(s) 119, 619, 824
XmaJI CCTAGG 1 cut(s) 646
XmiI GTMKAC 3 cut(s) 745, 1120, 1168
XmnI GAANNNNTTC 1 cut(s) 845
XspI CTAG 2 cut(s) 647, 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.