MD08G1108500.v1.1

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008052
Physical Location & Seq
Reverse (-)
9555985 .. 9559893
3909 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1108500.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGGCGACGATGGATGTGGAGATCGGCGGCGATGGAGTCGTGCGGAAGGAGGAGCCGCCCCCGAACGAGATGAAGCACAGAGGGAGCAAGAAAAACGCGGGGAAAAAGAAAAGGAGCAAAAGCAAGGGGACGATGACGACGAGCGGAAAAAAAGAAACGTTTTCTTACATGCCCAAGTTTCGGGGTTTCAGATCGAAACCCGAAACGGATAAGAATGGGTGTTTCGATATAGAGGTGGAGGATCAAACGGGTGAAAAAATGAGTCCGACCCATTTGATTATTTTGGTGAATGGTCTCATCGGCAGTGCTGAGAATTGGAAATATGCAGCAAAGGAGTTTCTGAAGAGATACCCAGAAGATGTTGTTGCTCACTGCAGTGAATGCAATTCTTCAATGTTAACATTTGATGGTGTTGATGTGATGGGAGAGAGATTAGCAGAGGAGGTTATTTCTGTGATAAAACGTCACCCGAGTGTACAGAAGATCTCGTTTGTTGGTCATTCGTTAGGTGGGCTTATAGCAAGATATGCCATTGGAAGGCTTTATGAACGAAACATCACCGGAGAACTATCTCAAGAAAATGGAGAACATAGGAGAGATGGAGTTGAGGATCCCTTGCTTAAACAAAAAGTCCAACGCAAAATTGCTGGACTGGAGCCCATGAATTTTATAACGTCTGCAACTCCGCACCTCGGTTCCTGGGGGCATAATCAGGTTCCAGTGTTTTGTGGTGTAAAACCCCTTGAAAAAGTAGCGGCTCGTACTTCATGGTGTCTAGGTAGAAGTGGAAGACATCTGTTTTTAACTGATAGGAATGATGGAAAACCTCCCCTTCTTCTTCAGATGGTCCATGACACTGAAGATCTCAAATTCATATCTGCACTGCAGTCCTTCAAGCGCCGTGTTGCCTATGCAAATGTTCGATTTGATCACCTTGTGGGATGGAGTACATCATCTCTACGGCGTAAGAAGGAGCTGCCAAAGCTTAAAAATTTATCCAGAGATGACAAATATCGACATATTGTAAATGTGGAGACAATTAAAAGTCCAAGTCCTCAAGAAGAATTACCGTTGGAAGCCATAGCCAGTGGGCCCGAGAAAATTGACTTCGAAGAGGAAATGATCAGAAACTTGACCAAGATGAGCTGGGAACGGGTGGATGTGAACTTCAGCAGAAGTAAACAAAGATACCTTGCACACAGTACCATTCAGGTGAAAAACACTTGTCTACACTCTGATGGGGCTGATGTGATCCAACACATGGTTGACAATTTTCTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

428

Amino Acids

48.32

Weight (kDa)

9.2

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 88 - 317 4.5e-65 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 671
AccB7I CCANNNNNTGG 1 cut(s) 1261
AccBSI CCGCTC 1 cut(s) 144
AccI GTMKAC 1 cut(s) 1228
AccII CGCG 1 cut(s) 98
AciI CCGC 7 cut(s) 27, 43, 56, 98, 144, 686, 755
AclI AACGTT 1 cut(s) 158
AclWI GGATC 4 cut(s) 249, 605, 618, 1246
AcsI RAATTY 3 cut(s) 664, 869, 991
AcuI CTGAAG 4 cut(s) 362, 824, 879, 1153
AdeI CACNNNGTG 1 cut(s) 937
AfaI GTAC 4 cut(s) 477, 763, 949, 1204
AfiI CCNNNNNNNGG 4 cut(s) 180, 537, 692, 1261
AgsI TTSAA 3 cut(s) 393, 746, 895
AjnI CCWGG 1 cut(s) 698
AleI CACNNNNGTG 2 cut(s) 375, 471
AluBI AGCT 3 cut(s) 976, 985, 1146
AluI AGCT 3 cut(s) 976, 985, 1146
Alw26I GTCTC 2 cut(s) 299, 1028
AlwI GGATC 4 cut(s) 249, 605, 618, 1246
Ama87I CYCGRG 2 cut(s) 469, 1094
AoxI GGCC 1 cut(s) 1091
ApaI GGGCCC 1 cut(s) 1095
ApeKI GCWGC 2 cut(s) 326, 976
ApoI RAATTY 3 cut(s) 664, 869, 991
AspLEI GCGC 1 cut(s) 900
AspS9I GGNCC 3 cut(s) 847, 1091, 1092
AsuHPI GGTGA 6 cut(s) 263, 298, 458, 550, 923, 1225
AsuII TTCGAA 1 cut(s) 1110
AvaI CYCGRG 2 cut(s) 469, 1094
AvaII GGWCC 1 cut(s) 847
BaeGI GKGCMC 1 cut(s) 1095
BamHI GGATCC 1 cut(s) 610
BanII GRGCYC 2 cut(s) 660, 1095
BbsI GAAGAC 1 cut(s) 796
BbvI GCAGC 2 cut(s) 338, 963
BccI CCATC 9 cut(s) 4, 26, 401, 415, 593, 812, 838, 936, 1232
BceAI ACGGC 2 cut(s) 885, 977
BciT130I CCWGG 1 cut(s) 700
BclI TGATCA 2 cut(s) 928, 1122
BcoDI GTCTC 2 cut(s) 299, 1028
BfaI CTAG 1 cut(s) 776
BfmI CTRYAG 2 cut(s) 373, 884
BfoI RGCGCY 1 cut(s) 901
BglII AGATCT 2 cut(s) 483, 862
BisI GCNGC 5 cut(s) 28, 56, 327, 756, 977
BlsI GCNGC 5 cut(s) 29, 57, 328, 757, 978
Bme1390I CCNGG 1 cut(s) 700
Bme18I GGWCC 1 cut(s) 847
BmeT110I CYCGRG 2 cut(s) 469, 1094
BmgT120I GGNCC 3 cut(s) 847, 1091, 1092
BmiI GGNNCC 6 cut(s) 54, 612, 657, 697, 717, 1093
BmrFI CCNGG 1 cut(s) 700
BpiI GAAGAC 1 cut(s) 796
BpmI CTGGAG 1 cut(s) 674
Bpu14I TTCGAA 1 cut(s) 1110
BpuEI CTTGAG 2 cut(s) 558, 1041
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 2 cut(s) 691, 699
BsaWI WCCGGW 1 cut(s) 560
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 537, 692, 1261
Bse1I ACTGG 3 cut(s) 657, 719, 1086
BseBI CCWGG 1 cut(s) 700
BseDI CCNNGG 2 cut(s) 691, 699
BseGI GGATG 3 cut(s) 19, 947, 1165
BseLI CCNNNNNNNGG 4 cut(s) 180, 537, 692, 1261
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 3 cut(s) 657, 719, 1086
BseRI GAGGAG 2 cut(s) 65, 455
BseSI GKGCMC 1 cut(s) 1095
BseXI GCAGC 2 cut(s) 338, 963
BseYI CCCAGC 1 cut(s) 1146
BsgI GTGCAG 1 cut(s) 864
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 1 cut(s) 1093
BsiHKCI CYCGRG 2 cut(s) 469, 1094
BsiSI CCGG 1 cut(s) 561
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 4 cut(s) 180, 537, 692, 1261
BsmAI GTCTC 2 cut(s) 299, 1028
BsmFI GGGAC 1 cut(s) 142
BsmI GAATGC 1 cut(s) 386
BsnI GGCC 1 cut(s) 1093
Bso31I GGTCTC 1 cut(s) 299
BsoBI CYCGRG 2 cut(s) 469, 1094
Bsp119I TTCGAA 1 cut(s) 1110
Bsp120I GGGCCC 1 cut(s) 1091
Bsp1286I GDGCHC 2 cut(s) 660, 1095
Bsp1407I TGTACA 1 cut(s) 475
Bsp143I GATC 9 cut(s) 21, 191, 241, 483, 610, 862, 928, 1122, 1251
BspACI CCGC 7 cut(s) 27, 43, 56, 98, 144, 686, 755
BspANI GGCC 1 cut(s) 1093
BspCNI CTCAG 1 cut(s) 301
BspFNI CGCG 1 cut(s) 98
BspLI GGNNCC 6 cut(s) 54, 612, 657, 697, 717, 1093
BspMAI CTGCAG 2 cut(s) 377, 888
BspPI GGATC 4 cut(s) 249, 605, 618, 1246
BspT104I TTCGAA 1 cut(s) 1110
BspTNI GGTCTC 1 cut(s) 299
BsrBI CCGCTC 1 cut(s) 144
BsrGI TGTACA 1 cut(s) 475
BsrI ACTGG 3 cut(s) 657, 719, 1086
BssECI CCNNGG 2 cut(s) 691, 699
BssMI GATC 9 cut(s) 21, 191, 241, 483, 610, 862, 928, 1122, 1251
Bst2UI CCWGG 1 cut(s) 700
Bst4CI ACNGT 2 cut(s) 1071, 1202
Bst6I CTCTTC 2 cut(s) 338, 1107
BstAPI GCANNNNNTGC 2 cut(s) 381, 527
BstAUI TGTACA 1 cut(s) 475
BstBI TTCGAA 1 cut(s) 1110
BstDEI CTNAG 1 cut(s) 309
BstF5I GGATG 3 cut(s) 19, 947, 1165
BstFNI CGCG 1 cut(s) 98
BstH2I RGCGCY 1 cut(s) 901
BstHHI GCGC 1 cut(s) 900
BstKTI GATC 9 cut(s) 24, 194, 244, 486, 613, 865, 931, 1125, 1254
BstMAI GTCTC 2 cut(s) 299, 1028
BstMBI GATC 9 cut(s) 21, 191, 241, 483, 610, 862, 928, 1122, 1251
BstMWI GCNNNNNNNGC 3 cut(s) 381, 527, 982
BstNI CCWGG 1 cut(s) 700
BstNSI RCATGY 1 cut(s) 172
BstSCI CCNGG 1 cut(s) 698
BstSFI CTRYAG 2 cut(s) 373, 884
BstSLI GKGCMC 1 cut(s) 1095
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 2 cut(s) 338, 963
BstV2I GAAGAC 1 cut(s) 796
BstX2I RGATCY 3 cut(s) 483, 610, 862
BstYI RGATCY 3 cut(s) 483, 610, 862
BsuRI GGCC 1 cut(s) 1093
BtgZI GCGATG 1 cut(s) 45
BtsCI GGATG 3 cut(s) 19, 947, 1165
BtsI GCAGTG 4 cut(s) 310, 370, 382, 881
BtsIMutI CAGTG 7 cut(s) 310, 370, 382, 726, 855, 881, 1093
CfoI GCGC 1 cut(s) 900
Cfr13I GGNCC 3 cut(s) 847, 1091, 1092
Csp6I GTAC 4 cut(s) 476, 762, 948, 1203
CviAII CATG 5 cut(s) 169, 661, 768, 851, 1261
CviQI GTAC 4 cut(s) 476, 762, 948, 1203
DdeI CTNAG 1 cut(s) 309
DpnI GATC 9 cut(s) 23, 193, 243, 485, 612, 864, 930, 1124, 1253
DpnII GATC 9 cut(s) 21, 191, 241, 483, 610, 862, 928, 1122, 1251
DraIII CACNNNGTG 1 cut(s) 937
Eam1104I CTCTTC 2 cut(s) 338, 1107
EarI CTCTTC 2 cut(s) 338, 1107
Eco24I GRGCYC 2 cut(s) 660, 1095
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 847
Eco57I CTGAAG 4 cut(s) 362, 824, 879, 1153
Eco88I CYCGRG 2 cut(s) 469, 1094
EcoRII CCWGG 1 cut(s) 698
EcoT38I GRGCYC 2 cut(s) 660, 1095
FaeI CATG 5 cut(s) 172, 664, 771, 854, 1264
FaqI GGGAC 1 cut(s) 142
FatI CATG 5 cut(s) 168, 660, 767, 850, 1260
FauI CCCGC 1 cut(s) 91
FbaI TGATCA 2 cut(s) 928, 1122
FblI GTMKAC 1 cut(s) 1228
Fnu4HI GCNGC 5 cut(s) 28, 56, 327, 756, 977
FokI GGATG 3 cut(s) 26, 954, 1172
FriOI GRGCYC 2 cut(s) 660, 1095
Fsp4HI GCNGC 5 cut(s) 28, 56, 327, 756, 977
FspBI CTAG 1 cut(s) 776
GlaI GCGC 1 cut(s) 899
GluI GCNGC 5 cut(s) 28, 56, 327, 756, 977
GsaI CCCAGC 1 cut(s) 1150
GsuI CTGGAG 1 cut(s) 674
HaeII RGCGCY 1 cut(s) 901
HaeIII GGCC 1 cut(s) 1093
HapII CCGG 1 cut(s) 561
HhaI GCGC 1 cut(s) 900
Hin1II CATG 5 cut(s) 172, 664, 771, 854, 1264
Hin6I GCGC 1 cut(s) 898
HinP1I GCGC 1 cut(s) 898
HincII GTYRAC 2 cut(s) 399, 1267
HindII GTYRAC 2 cut(s) 399, 1267
HindIII AAGCTT 1 cut(s) 983
HinfI GANTC 2 cut(s) 36, 262
HpaI GTTAAC 1 cut(s) 399
HpaII CCGG 1 cut(s) 561
HphI GGTGA 6 cut(s) 263, 298, 458, 550, 923, 1225
Hpy166II GTNNAC 6 cut(s) 399, 476, 1165, 1181, 1229, 1267
Hpy188I TCNGA 6 cut(s) 191, 267, 342, 843, 1127, 1237
Hpy188III TCNNGA 3 cut(s) 575, 999, 1058
Hpy8I GTNNAC 6 cut(s) 399, 476, 1165, 1181, 1229, 1267
Hpy99I CGWCG 2 cut(s) 10, 142
HpyAV CCTTC 5 cut(s) 40, 531, 842, 901, 964
HpyCH4III ACNGT 2 cut(s) 1071, 1202
HpyCH4IV ACGT 3 cut(s) 158, 463, 674
HpyCH4V TGCA 8 cut(s) 326, 375, 384, 680, 881, 886, 914, 1196
HpyF10VI GCNNNNNNNGC 3 cut(s) 381, 527, 982
HpyF3I CTNAG 1 cut(s) 309
HpySE526I ACGT 3 cut(s) 158, 463, 674
Hsp92II CATG 5 cut(s) 172, 664, 771, 854, 1264
HspAI GCGC 1 cut(s) 898
Ksp22I TGATCA 2 cut(s) 928, 1122
KspAI GTTAAC 1 cut(s) 399
Kzo9I GATC 9 cut(s) 21, 191, 241, 483, 610, 862, 928, 1122, 1251
LmnI GCTCC 5 cut(s) 52, 84, 114, 655, 973
Lsp1109I GCAGC 2 cut(s) 338, 963
MaeI CTAG 1 cut(s) 776
MaeII ACGT 3 cut(s) 158, 463, 674
MaeIII GTNAC 1 cut(s) 464
MalI GATC 9 cut(s) 23, 193, 243, 485, 612, 864, 930, 1124, 1253
MbiI CCGCTC 1 cut(s) 144
MboI GATC 9 cut(s) 21, 191, 241, 483, 610, 862, 928, 1122, 1251
MflI RGATCY 3 cut(s) 483, 610, 862
MhlI GDGCHC 2 cut(s) 660, 1095
MlyI GAGTC 2 cut(s) 45, 271
MmeI TCCRAC 4 cut(s) 290, 658, 1053, 1279
MseI TTAA 5 cut(s) 398, 621, 803, 987, 1041
MslI CAYNNNNRTG 4 cut(s) 375, 471, 1211, 1236
MspI CCGG 1 cut(s) 561
MspR9I CCNGG 1 cut(s) 700
Mva1269I GAATGC 1 cut(s) 386
MvaI CCWGG 1 cut(s) 700
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 3 cut(s) 381, 527, 982
NdeII GATC 9 cut(s) 21, 191, 241, 483, 610, 862, 928, 1122, 1251
NlaIII CATG 5 cut(s) 172, 664, 771, 854, 1264
NlaIV GGNNCC 6 cut(s) 54, 612, 657, 697, 717, 1093
NmuCI GTSAC 1 cut(s) 464
NspI RCATGY 1 cut(s) 172
NspV TTCGAA 1 cut(s) 1110
OliI CACNNNNGTG 2 cut(s) 375, 471
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PctI GAATGC 1 cut(s) 386
PflMI CCANNNNNTGG 1 cut(s) 1261
PkrI GCNGC 5 cut(s) 29, 57, 328, 757, 978
PleI GAGTC 2 cut(s) 44, 270
PpsI GAGTC 2 cut(s) 44, 270
PsiI TTATAA 1 cut(s) 671
Psp1406I AACGTT 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 698
PspFI CCCAGC 1 cut(s) 1146
PspGI CCWGG 1 cut(s) 698
PspN4I GGNNCC 6 cut(s) 54, 612, 657, 697, 717, 1093
PspOMI GGGCCC 1 cut(s) 1091
PspPI GGNCC 3 cut(s) 847, 1091, 1092
PstI CTGCAG 2 cut(s) 377, 888
PsuI RGATCY 3 cut(s) 483, 610, 862
RsaI GTAC 4 cut(s) 477, 763, 949, 1204
RsaNI GTAC 4 cut(s) 476, 762, 948, 1203
RseI CAYNNNNRTG 4 cut(s) 375, 471, 1211, 1236
SaqAI TTAA 5 cut(s) 398, 621, 803, 987, 1041
SatI GCNGC 5 cut(s) 28, 56, 327, 756, 977
Sau3AI GATC 9 cut(s) 21, 191, 241, 483, 610, 862, 928, 1122, 1251
Sau96I GGNCC 3 cut(s) 847, 1091, 1092
SchI GAGTC 2 cut(s) 45, 271
ScrFI CCNGG 1 cut(s) 700
SduI GDGCHC 2 cut(s) 660, 1095
SfcI CTRYAG 2 cut(s) 373, 884
SfuI TTCGAA 1 cut(s) 1110
SinI GGWCC 1 cut(s) 847
SmiMI CAYNNNNRTG 4 cut(s) 375, 471, 1211, 1236
SmlI CTYRAG 2 cut(s) 573, 1056
SmoI CTYRAG 2 cut(s) 573, 1056
SsiI CCGC 7 cut(s) 27, 43, 56, 98, 144, 686, 755
SspMI CTAG 1 cut(s) 776
StyD4I CCNGG 1 cut(s) 698
TaaI ACNGT 2 cut(s) 1071, 1202
TaiI ACGT 3 cut(s) 161, 466, 677
TaqI TCGA 5 cut(s) 194, 225, 922, 1015, 1110
TatI WGTACW 2 cut(s) 475, 947
TauI GCSGC 3 cut(s) 30, 58, 758
Tru1I TTAA 5 cut(s) 398, 621, 803, 987, 1041
Tru9I TTAA 5 cut(s) 398, 621, 803, 987, 1041
TscAI CASTG 7 cut(s) 310, 377, 382, 726, 862, 888, 1093
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 2 cut(s) 326, 976
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 5 cut(s) 86, 561, 677, 756, 862
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 7 cut(s) 310, 377, 382, 726, 862, 888, 1093
Van91I CCANNNNNTGG 1 cut(s) 1261
VpaK11BI GGWCC 1 cut(s) 847
XapI RAATTY 3 cut(s) 664, 869, 991
XceI RCATGY 1 cut(s) 172
XmiI GTMKAC 1 cut(s) 1228
XspI CTAG 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.