Rorug06G0354000

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004662
Physical Location & Seq
Forward (+)
50221963 .. 50224714
2752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0354000.1

Sequence Viewer

Length: 711 bp
ATGGACATGAAGCAATGCTCACACCATTATTGCGTTGCAACTAAGTACATTGATGATAAGTTGCTTCGTACAGTGAACCATATTGAGGGACTTAAGCAGGTTGTTTTGCTAACAGATGGCATGGACACTCGGCCGTATAGGCTTAGTTGGCCAACTTCAACCATTATTTTTGATATTTCGCCGGACCGGGTATTCAGTGGAGCAGCTGAAAAGCTTAAAGGTGTTGGGGCTAAAATCCCAAGAAGCTGCTTATTCCTTCATGTTCCATTGGAGTCCTCCAATTTGCAGCAAACTCTACGTGCTAGAGGCTTCAATGGTAACCGGCCTAGTATATGGGCCATGCAGGGACTGCCCGTAATGACATTGGCAAATTTTGAGGACATTTTGCTCACTGTAAGTGGTTTGTCCATGAATGGATGTGGCTTTTTGGGAGAATTGCCTGCTTGGTTGGCAGAGACTGAAATAGTGACCAAGACTAGTACAAGGGAATGGATGGAGAAATTATTCATGAACAATGGCTTTCGGGTGGACATGATTCACTATGAAGAAGTTGCAAAGAGCTTAGGCAAGAAATTAGCAGCAGGACCTTATAAGAATATACTATTTTCCTCAGAACAACTGCGGTTTTCTGATGATCAGATGGAAACTTGGAGAAGAGAATTTCAAAGAGTTGAGGAACAAGGGGATGAAGAAGGTTTTGAAGAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

236

Amino Acids

26.92

Weight (kDa)

5.62

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 3 - 117 1e-20 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 591
Acc36I ACCTGC 1 cut(s) 88
AciI CCGC 1 cut(s) 622
AcoI YGGCCR 2 cut(s) 131, 149
AcsI RAATTY 2 cut(s) 370, 659
AfaI GTAC 3 cut(s) 47, 70, 481
AfiI CCNNNNNNNGG 1 cut(s) 85
AflII CTTAAG 1 cut(s) 92
AgsI TTSAA 4 cut(s) 159, 313, 665, 701
AhlI ACTAGT 1 cut(s) 476
AluBI AGCT 5 cut(s) 206, 214, 246, 561, 706
AluI AGCT 5 cut(s) 206, 214, 246, 561, 706
Alw26I GTCTC 1 cut(s) 449
AlwNI CAGNNNCTG 2 cut(s) 349, 458
AoxI GGCC 4 cut(s) 131, 149, 323, 336
ApeKI GCWGC 4 cut(s) 203, 246, 286, 578
ApoI RAATTY 2 cut(s) 370, 659
Asp700I GAANNNNTTC 1 cut(s) 503
AspS9I GGNCC 3 cut(s) 184, 336, 584
AsuC2I CCSGG 1 cut(s) 188
AvaII GGWCC 2 cut(s) 184, 584
BalI TGGCCA 1 cut(s) 151
BbvI GCAGC 4 cut(s) 215, 233, 298, 590
BccI CCATC 3 cut(s) 110, 487, 634
BceAI ACGGC 1 cut(s) 118
BclI TGATCA 1 cut(s) 634
BcnI CCSGG 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 449
BcuI ACTAGT 1 cut(s) 476
BfaI CTAG 3 cut(s) 303, 327, 477
BfrI CTTAAG 1 cut(s) 92
BfuAI ACCTGC 1 cut(s) 88
BglI GCCNNNNNGGC 1 cut(s) 139
BisI GCNGC 4 cut(s) 204, 247, 287, 579
BlsI GCNGC 4 cut(s) 205, 248, 288, 580
Bme1390I CCNGG 1 cut(s) 188
Bme18I GGWCC 2 cut(s) 184, 584
BmgT120I GGNCC 3 cut(s) 184, 336, 584
BmrFI CCNGG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 562
BpuMI CCSGG 1 cut(s) 188
BsaAI YACGTR 1 cut(s) 299
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse118I RCCGGY 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 20
BseGI GGATG 3 cut(s) 422, 498, 691
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMI GCAATG 1 cut(s) 20
BseMII CTCAG 1 cut(s) 624
BseX3I CGGCCG 1 cut(s) 131
BseXI GCAGC 4 cut(s) 215, 233, 298, 590
Bsh1285I CGRYCG 1 cut(s) 134
BshFI GGCC 4 cut(s) 133, 151, 325, 338
BsiEI CGRYCG 1 cut(s) 134
BsiSI CCGG 3 cut(s) 182, 187, 322
BslFI GGGAC 2 cut(s) 102, 360
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 1 cut(s) 449
BsmFI GGGAC 2 cut(s) 102, 360
BsnI GGCC 4 cut(s) 133, 151, 325, 338
Bsp143I GATC 1 cut(s) 634
BspACI CCGC 1 cut(s) 622
BspANI GGCC 4 cut(s) 133, 151, 325, 338
BspCNI CTCAG 1 cut(s) 623
BspHI TCATGA 1 cut(s) 507
BspMI ACCTGC 1 cut(s) 88
BspQI GCTCTTC 1 cut(s) 696
BspTI CTTAAG 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 20
BsrFI RCCGGY 1 cut(s) 321
BssAI RCCGGY 1 cut(s) 321
BssMI GATC 1 cut(s) 634
Bst4CI ACNGT 2 cut(s) 73, 394
Bst6I CTCTTC 2 cut(s) 649, 696
BstAFI CTTAAG 1 cut(s) 92
BstAPI GCANNNNNTGC 1 cut(s) 349
BstBAI YACGTR 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 4 cut(s) 42, 143, 562, 610
BstEII GGTNACC 1 cut(s) 317
BstF5I GGATG 3 cut(s) 422, 498, 691
BstKTI GATC 1 cut(s) 637
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 1 cut(s) 634
BstMCI CGRYCG 1 cut(s) 134
BstMWI GCNNNNNNNGC 4 cut(s) 139, 148, 349, 449
BstPI GGTNACC 1 cut(s) 317
BstSCI CCNGG 1 cut(s) 186
BstV1I GCAGC 4 cut(s) 215, 233, 298, 590
BstZI CGGCCG 1 cut(s) 131
BsuRI GGCC 4 cut(s) 133, 151, 325, 338
BtsCI GGATG 3 cut(s) 422, 498, 691
BtsIMutI CAGTG 3 cut(s) 78, 202, 390
BveI ACCTGC 1 cut(s) 88
Cac8I GCNNGC 1 cut(s) 441
CaiI CAGNNNCTG 2 cut(s) 349, 458
CciI TCATGA 1 cut(s) 507
Cfr10I RCCGGY 1 cut(s) 321
Cfr13I GGNCC 3 cut(s) 184, 336, 584
CpoI CGGWCCG 1 cut(s) 184
Csp6I GTAC 3 cut(s) 46, 69, 480
CspI CGGWCCG 1 cut(s) 184
CviAII CATG 7 cut(s) 7, 121, 260, 340, 409, 508, 532
CviQI GTAC 3 cut(s) 46, 69, 480
DdeI CTNAG 4 cut(s) 42, 143, 562, 610
DpnI GATC 1 cut(s) 636
DpnII GATC 1 cut(s) 634
EaeI YGGCCR 2 cut(s) 131, 149
EagI CGGCCG 1 cut(s) 131
Eam1104I CTCTTC 2 cut(s) 649, 696
EarI CTCTTC 2 cut(s) 649, 696
EclXI CGGCCG 1 cut(s) 131
Eco47I GGWCC 2 cut(s) 184, 584
Eco52I CGGCCG 1 cut(s) 131
Eco91I GGTNACC 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 584
EcoO65I GGTNACC 1 cut(s) 317
FaeI CATG 7 cut(s) 10, 124, 263, 343, 412, 511, 535
FaqI GGGAC 2 cut(s) 102, 360
FatI CATG 7 cut(s) 6, 120, 259, 339, 408, 507, 531
FbaI TGATCA 1 cut(s) 634
Fnu4HI GCNGC 4 cut(s) 204, 247, 287, 579
FokI GGATG 3 cut(s) 429, 505, 698
Fsp4HI GCNGC 4 cut(s) 204, 247, 287, 579
FspBI CTAG 3 cut(s) 303, 327, 477
GluI GCNGC 4 cut(s) 204, 247, 287, 579
HaeIII GGCC 4 cut(s) 133, 151, 325, 338
HapII CCGG 3 cut(s) 182, 187, 322
Hin1II CATG 7 cut(s) 10, 124, 263, 343, 412, 511, 535
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 2 cut(s) 272, 535
HpaII CCGG 3 cut(s) 182, 187, 322
Hpy166II GTNNAC 2 cut(s) 76, 529
Hpy188I TCNGA 3 cut(s) 613, 631, 639
Hpy188III TCNNGA 1 cut(s) 508
Hpy8I GTNNAC 2 cut(s) 76, 529
HpyAV CCTTC 2 cut(s) 266, 686
HpyCH4III ACNGT 2 cut(s) 73, 394
HpyCH4IV ACGT 1 cut(s) 298
HpyCH4V TGCA 4 cut(s) 38, 286, 343, 554
HpyF10VI GCNNNNNNNGC 4 cut(s) 139, 148, 349, 449
HpyF3I CTNAG 4 cut(s) 42, 143, 562, 610
HpySE526I ACGT 1 cut(s) 298
Hsp92II CATG 7 cut(s) 10, 124, 263, 343, 412, 511, 535
Ksp22I TGATCA 1 cut(s) 634
Kzo9I GATC 1 cut(s) 634
LguI GCTCTTC 1 cut(s) 696
LmnI GCTCC 1 cut(s) 200
LpnPI CCDG 7 cut(s) 83, 195, 200, 329, 335, 453, 567
Lsp1109I GCAGC 4 cut(s) 215, 233, 298, 590
MaeI CTAG 3 cut(s) 303, 327, 477
MaeII ACGT 1 cut(s) 298
MaeIII GTNAC 2 cut(s) 317, 466
MalI GATC 1 cut(s) 636
MboI GATC 1 cut(s) 634
MboII GAAGA 3 cut(s) 557, 666, 701
MlsI TGGCCA 1 cut(s) 151
MluCI AATT 6 cut(s) 280, 370, 434, 500, 572, 659
MluNI TGGCCA 1 cut(s) 151
MlyI GAGTC 1 cut(s) 281
MnlI CCTC 6 cut(s) 79, 286, 299, 370, 619, 667
Mox20I TGGCCA 1 cut(s) 151
MroXI GAANNNNTTC 1 cut(s) 503
MscI TGGCCA 1 cut(s) 151
MseI TTAA 2 cut(s) 93, 216
Msp20I TGGCCA 1 cut(s) 151
MspA1I CMGCKG 1 cut(s) 206
MspCI CTTAAG 1 cut(s) 92
MspI CCGG 3 cut(s) 182, 187, 322
MspR9I CCNGG 1 cut(s) 188
MwoI GCNNNNNNNGC 4 cut(s) 139, 148, 349, 449
NciI CCSGG 1 cut(s) 188
NdeII GATC 1 cut(s) 634
NlaIII CATG 7 cut(s) 10, 124, 263, 343, 412, 511, 535
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 1 cut(s) 466
PagI TCATGA 1 cut(s) 507
PciSI GCTCTTC 1 cut(s) 696
PdmI GAANNNNTTC 1 cut(s) 503
PfeI GAWTC 1 cut(s) 535
PkrI GCNGC 4 cut(s) 205, 248, 288, 580
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
Ppu21I YACGTR 1 cut(s) 299
PpuMI RGGWCCY 1 cut(s) 584
PsiI TTATAA 1 cut(s) 591
Psp5II RGGWCCY 1 cut(s) 584
PspEI GGTNACC 1 cut(s) 317
PspPI GGNCC 3 cut(s) 184, 336, 584
PspPPI RGGWCCY 1 cut(s) 584
PstNI CAGNNNCTG 2 cut(s) 349, 458
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 3 cut(s) 47, 70, 481
RsaNI GTAC 3 cut(s) 46, 69, 480
Rsr2I CGGWCCG 1 cut(s) 184
RsrII CGGWCCG 1 cut(s) 184
SapI GCTCTTC 1 cut(s) 696
SaqAI TTAA 2 cut(s) 93, 216
SatI GCNGC 4 cut(s) 204, 247, 287, 579
Sau3AI GATC 1 cut(s) 634
Sau96I GGNCC 3 cut(s) 184, 336, 584
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 1 cut(s) 188
SinI GGWCC 2 cut(s) 184, 584
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
SpeI ACTAGT 1 cut(s) 476
Sse9I AATT 6 cut(s) 280, 370, 434, 500, 572, 659
SsiI CCGC 1 cut(s) 622
SspMI CTAG 3 cut(s) 303, 327, 477
StyD4I CCNGG 1 cut(s) 186
TaaI ACNGT 2 cut(s) 73, 394
TaiI ACGT 1 cut(s) 301
TasI AATT 6 cut(s) 280, 370, 434, 500, 572, 659
TatI WGTACW 2 cut(s) 45, 479
TfiI GAWTC 1 cut(s) 535
Tru1I TTAA 2 cut(s) 93, 216
Tru9I TTAA 2 cut(s) 93, 216
TscAI CASTG 3 cut(s) 78, 202, 397
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 4 cut(s) 203, 246, 286, 578
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 7 cut(s) 23, 248, 425, 496, 524, 558, 702
TspRI CASTG 3 cut(s) 78, 202, 397
Vha464I CTTAAG 1 cut(s) 92
VpaK11BI GGWCC 2 cut(s) 184, 584
XapI RAATTY 2 cut(s) 370, 659
XmnI GAANNNNTTC 1 cut(s) 503
XspI CTAG 3 cut(s) 303, 327, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.