RLG00000017730

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004662
Physical Location & Seq
Forward (+)
20546488 .. 20547109
622 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017730

Sequence Viewer

Length: 363 bp
ATGGGTTTTCGCTACACTTCTATCATCTTTCTGATGATTTTGAGCTCTACCCAGATAAGATTCATGGCTGAAAGCAGAGTACTTCTCAAGAAGGATGACACTTCCCAGACAGTGAATGAAGATAGTAAGGCAAGACTGAAAGCACAAATTGGTTCAAGGCCTCCAAGGTGTGAAGGAAGGTGCAGCTCATGTGGCCCTTGTGAGGCAATTCAAGTTCCTAACAATTTTCAAGCCAAACCCGGTAAAAGGAACTCTTCATCAGTTGTATCCAACATTGCATATGCCAGAGGAGGCGAGTATTCAAGCAACTACAAGCCCATGAGTTGGAAATGCAAATGTGGCACCATCATCTTCAACCCCTGA

Protein Analysis

121

Amino Acids

13.34

Weight (kDa)

9.66

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 50 - 120 7.5e-18 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 341
AccB7I CCANNNNNTGG 1 cut(s) 324
AfaI GTAC 1 cut(s) 81
AfiI CCNNNNNNNGG 3 cut(s) 202, 246, 324
AgsI TTSAA 5 cut(s) 156, 212, 230, 303, 355
AluBI AGCT 2 cut(s) 45, 186
AluI AGCT 2 cut(s) 45, 186
Alw21I GWGCWC 1 cut(s) 47
AoxI GGCC 2 cut(s) 158, 193
ApeKI GCWGC 1 cut(s) 183
AspS9I GGNCC 1 cut(s) 194
AsuC2I CCSGG 1 cut(s) 240
BanI GGYRCC 1 cut(s) 341
BanII GRGCYC 1 cut(s) 47
Bbv12I GWGCWC 1 cut(s) 47
BbvI GCAGC 1 cut(s) 195
BccI CCATC 1 cut(s) 353
BciVI GTATCC 1 cut(s) 277
BcnI CCSGG 1 cut(s) 240
BfuI GTATCC 1 cut(s) 277
BisI GCNGC 1 cut(s) 184
BlsI GCNGC 1 cut(s) 185
BmcAI AGTACT 1 cut(s) 81
Bme1390I CCNGG 1 cut(s) 240
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 1 cut(s) 343
BmrFI CCNGG 1 cut(s) 240
BplI GAGNNNNNCTC 2 cut(s) 69, 101
BpuEI CTTGAG 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 240
BsaJI CCNNGG 1 cut(s) 164
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 246, 324
Bse3DI GCAATG 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 164
BseGI GGATG 1 cut(s) 100
BseLI CCNNNNNNNGG 3 cut(s) 202, 246, 324
BseMI GCAATG 1 cut(s) 273
BseRI GAGGAG 1 cut(s) 303
BseXI GCAGC 1 cut(s) 195
BsgI GTGCAG 1 cut(s) 202
BshFI GGCC 2 cut(s) 160, 195
BshNI GGYRCC 1 cut(s) 341
BsiHKAI GWGCWC 1 cut(s) 47
BsiSI CCGG 1 cut(s) 240
BslI CCNNNNNNNGG 3 cut(s) 202, 246, 324
BsnI GGCC 2 cut(s) 160, 195
Bsp1286I GDGCHC 1 cut(s) 47
BspANI GGCC 2 cut(s) 160, 195
BspLI GGNNCC 1 cut(s) 343
BspT107I GGYRCC 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 273
BssECI CCNNGG 1 cut(s) 164
BssT1I CCWWGG 1 cut(s) 164
Bst4CI ACNGT 1 cut(s) 112
Bst6I CTCTTC 1 cut(s) 259
BstF5I GGATG 1 cut(s) 100
BstMWI GCNNNNNNNGC 2 cut(s) 192, 339
BstSCI CCNGG 1 cut(s) 238
BstV1I GCAGC 1 cut(s) 195
BsuI GTATCC 1 cut(s) 277
BsuRI GGCC 2 cut(s) 160, 195
BtsCI GGATG 1 cut(s) 100
BtsIMutI CAGTG 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 194
Csp6I GTAC 1 cut(s) 80
CviAII CATG 3 cut(s) 64, 189, 319
CviJI RGCY 7 cut(s) 45, 68, 160, 186, 195, 233, 316
CviKI_1 RGCY 7 cut(s) 45, 68, 160, 186, 195, 233, 316
CviQI GTAC 1 cut(s) 80
Eam1104I CTCTTC 1 cut(s) 259
EarI CTCTTC 1 cut(s) 259
Ecl136II GAGCTC 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 164
Eco147I AGGCCT 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 47
Eco53kI GAGCTC 1 cut(s) 45
EcoICRI GAGCTC 1 cut(s) 45
EcoT14I CCWWGG 1 cut(s) 164
EcoT38I GRGCYC 1 cut(s) 47
ErhI CCWWGG 1 cut(s) 164
FaeI CATG 3 cut(s) 67, 192, 322
FaiI YATR 5 cut(s) 65, 190, 280, 282, 320
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FatI CATG 3 cut(s) 63, 188, 318
FauNDI CATATG 1 cut(s) 280
Fnu4HI GCNGC 1 cut(s) 184
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 1 cut(s) 184
GluI GCNGC 1 cut(s) 184
HaeIII GGCC 2 cut(s) 160, 195
HapII CCGG 1 cut(s) 240
Hin1II CATG 3 cut(s) 67, 192, 322
HinfI GANTC 1 cut(s) 60
HpaII CCGG 1 cut(s) 240
Hpy188I TCNGA 1 cut(s) 33
Hpy188III TCNNGA 1 cut(s) 88
HpyAV CCTTC 3 cut(s) 85, 167, 171
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4V TGCA 3 cut(s) 183, 278, 333
HpyF10VI GCNNNNNNNGC 2 cut(s) 192, 339
Hsp92II CATG 3 cut(s) 67, 192, 322
LpnPI CCDG 4 cut(s) 65, 119, 253, 298
Lsp1109I GCAGC 1 cut(s) 195
MboII GAAGA 3 cut(s) 131, 246, 343
MhlI GDGCHC 1 cut(s) 47
MluCI AATT 3 cut(s) 147, 207, 223
MmeI TCCRAC 2 cut(s) 294, 305
MnlI CCTC 4 cut(s) 171, 196, 281, 284
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 1 cut(s) 240
MwoI GCNNNNNNNGC 2 cut(s) 192, 339
NciI CCSGG 1 cut(s) 240
NdeI CATATG 1 cut(s) 280
NlaIII CATG 3 cut(s) 67, 192, 322
NlaIV GGNNCC 1 cut(s) 343
PceI AGGCCT 1 cut(s) 160
PfeI GAWTC 1 cut(s) 60
PflMI CCANNNNNTGG 1 cut(s) 324
PkrI GCNGC 1 cut(s) 185
Psp124BI GAGCTC 1 cut(s) 47
PspN4I GGNNCC 1 cut(s) 343
PspPI GGNCC 1 cut(s) 194
RsaI GTAC 1 cut(s) 81
RsaNI GTAC 1 cut(s) 80
SacI GAGCTC 1 cut(s) 47
SatI GCNGC 1 cut(s) 184
Sau96I GGNCC 1 cut(s) 194
ScaI AGTACT 1 cut(s) 81
ScrFI CCNGG 1 cut(s) 240
SduI GDGCHC 1 cut(s) 47
SetI ASST 4 cut(s) 47, 170, 182, 188
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 3 cut(s) 147, 207, 223
SseBI AGGCCT 1 cut(s) 160
SstI GAGCTC 1 cut(s) 47
StuI AGGCCT 1 cut(s) 160
StyD4I CCNGG 1 cut(s) 238
StyI CCWWGG 1 cut(s) 164
TaaI ACNGT 1 cut(s) 112
TasI AATT 3 cut(s) 147, 207, 223
TatI WGTACW 1 cut(s) 79
TfiI GAWTC 1 cut(s) 60
TscAI CASTG 1 cut(s) 117
TseI GCWGC 1 cut(s) 183
TspDTI ATGAA 3 cut(s) 52, 132, 246
TspRI CASTG 1 cut(s) 117
Van91I CCANNNNNTGG 1 cut(s) 324
ZrmI AGTACT 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.