Rroxscaffold_2G00134340

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004662
Physical Location & Seq
Reverse (-)
71831981 .. 71832919
939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00134340.1

Sequence Viewer

Length: 372 bp
ATGGGTTTTCGCTACACCACCAGTTCTATCATCTTTCTGATGATTTTGAGCTCTACCCAGATAAGATTCATGGCTGAAAGCAGAGTACTTCTCAAGAAAGATGACACTTCCCAGCAGACAGTGAATGAAGACAATAAGGCAAGACTGAGAGCACAAATTGGTTCAAGGCCTCCAAGGTGTGAAGGAAGGTGCAGCTCATGTGGCCCTTGTGAGGCAATTCAAGTTCCTAACAATTTTCAAGCCAAACCAGGTAAAAGGAACTCTTCATCAGTTGTATCCAACATTGCATATGCCAGAGGAGGCGAGTATTCAAGCAACTACAAGCCCATGAGTTGGAAATGCAAATGTGGCACCATCATCTTCAACCCCTGA

Protein Analysis

123

Amino Acids

13.71

Weight (kDa)

9.69

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 53 - 123 8.1e-18 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 350
AccB7I CCANNNNNTGG 1 cut(s) 333
AfaI GTAC 1 cut(s) 87
AfiI CCNNNNNNNGG 2 cut(s) 211, 333
AgsI TTSAA 5 cut(s) 165, 221, 239, 312, 364
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 2 cut(s) 51, 195
AluI AGCT 2 cut(s) 51, 195
Alw21I GWGCWC 2 cut(s) 53, 154
AoxI GGCC 2 cut(s) 167, 202
ApeKI GCWGC 1 cut(s) 192
AspS9I GGNCC 1 cut(s) 203
BanI GGYRCC 1 cut(s) 350
BanII GRGCYC 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 2 cut(s) 53, 154
BbvI GCAGC 1 cut(s) 204
BccI CCATC 1 cut(s) 362
BciT130I CCWGG 1 cut(s) 249
BciVI GTATCC 1 cut(s) 286
BfuI GTATCC 1 cut(s) 286
BisI GCNGC 1 cut(s) 193
BlsI GCNGC 1 cut(s) 194
BmcAI AGTACT 1 cut(s) 87
Bme1390I CCNGG 1 cut(s) 249
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 1 cut(s) 249
BpiI GAAGAC 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 75, 107
BpuEI CTTGAG 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 173
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 333
Bse1I ACTGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 282
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 173
BseLI CCNNNNNNNGG 2 cut(s) 211, 333
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 1 cut(s) 137
BseNI ACTGG 1 cut(s) 21
BseRI GAGGAG 1 cut(s) 312
BseXI GCAGC 1 cut(s) 204
BseYI CCCAGC 1 cut(s) 111
BsgI GTGCAG 1 cut(s) 211
BshFI GGCC 2 cut(s) 169, 204
BshNI GGYRCC 1 cut(s) 350
BsiHKAI GWGCWC 2 cut(s) 53, 154
BslI CCNNNNNNNGG 2 cut(s) 211, 333
BsnI GGCC 2 cut(s) 169, 204
Bsp1286I GDGCHC 2 cut(s) 53, 154
BspANI GGCC 2 cut(s) 169, 204
BspCNI CTCAG 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 352
BspT107I GGYRCC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 282
BsrI ACTGG 1 cut(s) 21
BssECI CCNNGG 1 cut(s) 173
BssT1I CCWWGG 1 cut(s) 173
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 1 cut(s) 121
Bst6I CTCTTC 1 cut(s) 268
BstDEI CTNAG 1 cut(s) 146
BstMWI GCNNNNNNNGC 2 cut(s) 201, 348
BstNI CCWGG 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 247
BstV1I GCAGC 1 cut(s) 204
BstV2I GAAGAC 1 cut(s) 135
BsuI GTATCC 1 cut(s) 286
BsuRI GGCC 2 cut(s) 169, 204
BtsIMutI CAGTG 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 203
CsiI ACCWGGT 1 cut(s) 247
Csp6I GTAC 1 cut(s) 86
CviAII CATG 3 cut(s) 70, 198, 328
CviJI RGCY 7 cut(s) 51, 74, 169, 195, 204, 242, 325
CviKI_1 RGCY 7 cut(s) 51, 74, 169, 195, 204, 242, 325
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 1 cut(s) 146
Eam1104I CTCTTC 1 cut(s) 268
EarI CTCTTC 1 cut(s) 268
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 173
Eco147I AGGCCT 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 53
Eco53kI GAGCTC 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 51
EcoRII CCWGG 1 cut(s) 247
EcoT14I CCWWGG 1 cut(s) 173
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 173
FaeI CATG 3 cut(s) 73, 201, 331
FaiI YATR 5 cut(s) 71, 199, 289, 291, 329
FalI AAGNNNNNCTT 2 cut(s) 247, 279
FatI CATG 3 cut(s) 69, 197, 327
FauNDI CATATG 1 cut(s) 289
Fnu4HI GCNGC 1 cut(s) 193
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 1 cut(s) 193
GluI GCNGC 1 cut(s) 193
GsaI CCCAGC 1 cut(s) 115
HaeIII GGCC 2 cut(s) 169, 204
Hin1II CATG 3 cut(s) 73, 201, 331
HinfI GANTC 1 cut(s) 66
Hpy188I TCNGA 1 cut(s) 39
Hpy188III TCNNGA 1 cut(s) 94
HpyAV CCTTC 2 cut(s) 176, 180
HpyCH4III ACNGT 1 cut(s) 121
HpyCH4V TGCA 3 cut(s) 192, 287, 342
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 348
HpyF3I CTNAG 1 cut(s) 146
Hsp92II CATG 3 cut(s) 73, 201, 331
LpnPI CCDG 6 cut(s) 34, 71, 125, 234, 261, 307
Lsp1109I GCAGC 1 cut(s) 204
MabI ACCWGGT 1 cut(s) 247
MboII GAAGA 3 cut(s) 140, 255, 352
MhlI GDGCHC 2 cut(s) 53, 154
MluCI AATT 3 cut(s) 156, 216, 232
MmeI TCCRAC 2 cut(s) 303, 314
MnlI CCTC 4 cut(s) 180, 205, 290, 293
MspR9I CCNGG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 2 cut(s) 201, 348
NdeI CATATG 1 cut(s) 289
NlaIII CATG 3 cut(s) 73, 201, 331
NlaIV GGNNCC 1 cut(s) 352
PceI AGGCCT 1 cut(s) 169
PfeI GAWTC 1 cut(s) 66
PflMI CCANNNNNTGG 1 cut(s) 333
PkrI GCNGC 1 cut(s) 194
Psp124BI GAGCTC 1 cut(s) 53
Psp6I CCWGG 1 cut(s) 247
PspFI CCCAGC 1 cut(s) 111
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 1 cut(s) 352
PspPI GGNCC 1 cut(s) 203
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
SacI GAGCTC 1 cut(s) 53
SatI GCNGC 1 cut(s) 193
Sau96I GGNCC 1 cut(s) 203
ScaI AGTACT 1 cut(s) 87
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 2 cut(s) 53, 154
SetI ASST 5 cut(s) 53, 179, 191, 197, 253
SexAI ACCWGGT 1 cut(s) 247
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 3 cut(s) 156, 216, 232
SseBI AGGCCT 1 cut(s) 169
SstI GAGCTC 1 cut(s) 53
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 1 cut(s) 247
StyI CCWWGG 1 cut(s) 173
TaaI ACNGT 1 cut(s) 121
TasI AATT 3 cut(s) 156, 216, 232
TatI WGTACW 1 cut(s) 85
TfiI GAWTC 1 cut(s) 66
TscAI CASTG 1 cut(s) 126
TseI GCWGC 1 cut(s) 192
TspDTI ATGAA 3 cut(s) 58, 141, 255
TspRI CASTG 1 cut(s) 126
Van91I CCANNNNNTGG 1 cut(s) 333
ZrmI AGTACT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.