RLG00000010758

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004662
Physical Location & Seq
Reverse (-)
4167286 .. 4168429
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010758

Sequence Viewer

Length: 468 bp
ATGGGCAGCTCTCAAAACTGGGTTTGTTGGCACAGAAATAGACACGTAATCATTCCCATCCTACTAGTTTTGGTTTCAAGCTGCTTCAGTACCCACTTCAGTTTCATAGCACAAGCTACTAGACCAATTCCAACCAAGCTACCTGAAGCAACTACAGGAGGCAATGTTTTGAACGAGGAAAATGCTGGTGGAGGGGAAAGGGTCAACGCTCGTCAAATTGGGTCAAGGCCGCCGCAATGCCAGAGGCGGTGCGGTGGTTGTGGGCATTGTGTGGCGGTGCAGGTCCCGGTGGCTCCCCAAGTTCAACAAAGCCACAACAATGGAGCAAAACGCAGCAGCAGCTCTGCTGCTTCTACTATAAAAACAAGCCCCAAGAGCACTGCTTATTCCAGAGGAGGCGACGAGTTGTCGAATTACAAGCCCATAAGCTGGAGATGCAAGTGTGGAGATTTGTTCTTCAATCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

156

Amino Acids

16.75

Weight (kDa)

9.64

Isoelectric Point (pI)

54.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 73 - 155 2.2e-15 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 271
AccB7I CCANNNNNTGG 1 cut(s) 429
AciI CCGC 5 cut(s) 230, 233, 247, 252, 275
AcuI CTGAAG 3 cut(s) 70, 82, 165
AfaI GTAC 1 cut(s) 91
AfiI CCNNNNNNNGG 1 cut(s) 429
AflIII ACRYGT 1 cut(s) 43
AgsI TTSAA 4 cut(s) 78, 172, 305, 460
AhdI GACNNNNNGTC 1 cut(s) 406
AhlI ACTAGT 1 cut(s) 64
AluBI AGCT 6 cut(s) 9, 81, 116, 139, 342, 429
AluI AGCT 6 cut(s) 9, 81, 116, 139, 342, 429
Alw21I GWGCWC 1 cut(s) 380
AoxI GGCC 1 cut(s) 227
ApeKI GCWGC 6 cut(s) 6, 81, 333, 336, 339, 347
AspS9I GGNCC 1 cut(s) 283
AsuC2I CCSGG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 283
Bbv12I GWGCWC 1 cut(s) 380
BbvI GCAGC 6 cut(s) 18, 68, 334, 345, 348, 351
BccI CCATC 1 cut(s) 65
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BcnI CCSGG 1 cut(s) 287
BcuI ACTAGT 1 cut(s) 64
BfaI CTAG 2 cut(s) 65, 120
BfmI CTRYAG 1 cut(s) 153
BfuAI ACCTGC 1 cut(s) 271
BisI GCNGC 8 cut(s) 7, 82, 230, 233, 334, 337, 340, 348
BlsI GCNGC 8 cut(s) 8, 83, 231, 234, 335, 338, 341, 349
Bme1390I CCNGG 1 cut(s) 287
Bme18I GGWCC 1 cut(s) 283
BmeRI GACNNNNNGTC 1 cut(s) 406
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 2 cut(s) 285, 294
BmrFI CCNGG 1 cut(s) 287
BmrI ACTGGG 1 cut(s) 28
BmsI GCATC 1 cut(s) 425
BmuI ACTGGG 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 451
BpuMI CCSGG 1 cut(s) 287
BsaAI YACGTR 1 cut(s) 46
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 1 cut(s) 429
Bse1I ACTGG 1 cut(s) 23
Bse3DI GCAATG 2 cut(s) 169, 242
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 429
BseMI GCAATG 2 cut(s) 169, 242
BseNI ACTGG 1 cut(s) 23
BseRI GAGGAG 1 cut(s) 408
BseXI GCAGC 6 cut(s) 18, 68, 334, 345, 348, 351
BsgI GTGCAG 1 cut(s) 299
BshFI GGCC 1 cut(s) 229
BsiHKAI GWGCWC 1 cut(s) 380
BsiSI CCGG 1 cut(s) 287
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 269
BsnI GGCC 1 cut(s) 229
Bsp1286I GDGCHC 1 cut(s) 380
BspACI CCGC 5 cut(s) 230, 233, 247, 252, 275
BspANI GGCC 1 cut(s) 229
BspLI GGNNCC 2 cut(s) 285, 294
BspMI ACCTGC 1 cut(s) 271
BsrDI GCAATG 2 cut(s) 169, 242
BsrI ACTGG 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 46
BstF5I GGATG 1 cut(s) 57
BstMWI GCNNNNNNNGC 3 cut(s) 339, 375, 435
BstSCI CCNGG 1 cut(s) 285
BstSFI CTRYAG 1 cut(s) 153
BstV1I GCAGC 6 cut(s) 18, 68, 334, 345, 348, 351
BstXI CCANNNNNNTGG 1 cut(s) 320
BsuRI GGCC 1 cut(s) 229
BtsCI GGATG 1 cut(s) 57
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 1 cut(s) 378
BveI ACCTGC 1 cut(s) 271
Cfr13I GGNCC 1 cut(s) 283
Csp6I GTAC 1 cut(s) 90
CviQI GTAC 1 cut(s) 90
DriI GACNNNNNGTC 1 cut(s) 406
Eam1105I GACNNNNNGTC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 283
Eco57I CTGAAG 3 cut(s) 70, 82, 165
EcoO109I RGGNCCY 1 cut(s) 283
FaiI YATR 3 cut(s) 107, 359, 425
FaqI GGGAC 1 cut(s) 269
Fnu4HI GCNGC 8 cut(s) 7, 82, 230, 233, 334, 337, 340, 348
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 8 cut(s) 7, 82, 230, 233, 334, 337, 340, 348
FspBI CTAG 2 cut(s) 65, 120
GluI GCNGC 8 cut(s) 7, 82, 230, 233, 334, 337, 340, 348
GsuI CTGGAG 1 cut(s) 451
HaeIII GGCC 1 cut(s) 229
HapII CCGG 1 cut(s) 287
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HpaII CCGG 1 cut(s) 287
Hpy166II GTNNAC 1 cut(s) 205
Hpy188III TCNNGA 1 cut(s) 390
Hpy8I GTNNAC 1 cut(s) 205
Hpy99I CGWCG 1 cut(s) 404
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 2 cut(s) 280, 438
HpyF10VI GCNNNNNNNGC 3 cut(s) 339, 375, 435
HpySE526I ACGT 1 cut(s) 45
LmnI GCTCC 2 cut(s) 298, 323
LpnPI CCDG 9 cut(s) 4, 141, 156, 171, 254, 266, 300, 403, 415
Lsp1109I GCAGC 6 cut(s) 18, 68, 334, 345, 348, 351
LweI GCATC 1 cut(s) 425
MaeI CTAG 2 cut(s) 65, 120
MaeII ACGT 1 cut(s) 45
MboII GAAGA 1 cut(s) 448
MhlI GDGCHC 1 cut(s) 380
MluCI AATT 3 cut(s) 126, 216, 412
MmeI TCCRAC 1 cut(s) 155
MnlI CCTC 6 cut(s) 152, 169, 185, 237, 386, 389
MslI CAYNNNNRTG 1 cut(s) 318
MspI CCGG 1 cut(s) 287
MspR9I CCNGG 1 cut(s) 287
MwoI GCNNNNNNNGC 3 cut(s) 339, 375, 435
NciI CCSGG 1 cut(s) 287
NlaIV GGNNCC 2 cut(s) 285, 294
PflMI CCANNNNNTGG 1 cut(s) 429
PkrI GCNGC 8 cut(s) 8, 83, 231, 234, 335, 338, 341, 349
Ppu21I YACGTR 1 cut(s) 46
PpuMI RGGWCCY 1 cut(s) 283
Psp5II RGGWCCY 1 cut(s) 283
PspN4I GGNNCC 2 cut(s) 285, 294
PspPI GGNCC 1 cut(s) 283
PspPPI RGGWCCY 1 cut(s) 283
RsaI GTAC 1 cut(s) 91
RsaNI GTAC 1 cut(s) 90
RseI CAYNNNNRTG 1 cut(s) 318
SatI GCNGC 8 cut(s) 7, 82, 230, 233, 334, 337, 340, 348
Sau96I GGNCC 1 cut(s) 283
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 1 cut(s) 380
SetI ASST 9 cut(s) 11, 48, 83, 118, 141, 145, 285, 344, 431
SfaNI GCATC 1 cut(s) 425
SfcI CTRYAG 1 cut(s) 153
SinI GGWCC 1 cut(s) 283
SmiMI CAYNNNNRTG 1 cut(s) 318
SpeI ACTAGT 1 cut(s) 64
Sse9I AATT 3 cut(s) 126, 216, 412
SsiI CCGC 5 cut(s) 230, 233, 247, 252, 275
SspMI CTAG 2 cut(s) 65, 120
StyD4I CCNGG 1 cut(s) 285
TaiI ACGT 1 cut(s) 48
TaqI TCGA 1 cut(s) 410
TasI AATT 3 cut(s) 126, 216, 412
TauI GCSGC 2 cut(s) 232, 235
TscAI CASTG 1 cut(s) 385
TseI GCWGC 6 cut(s) 6, 81, 333, 336, 339, 347
TspDTI ATGAA 1 cut(s) 94
TspRI CASTG 1 cut(s) 385
Van91I CCANNNNNTGG 1 cut(s) 429
VpaK11BI GGWCC 1 cut(s) 283
XspI CTAG 2 cut(s) 65, 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.