FvH4_2g35090

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004662
Physical Location & Seq
Reverse (-)
25942190 .. 25943616
1427 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35090.t1

Sequence Viewer

Length: 447 bp
ATGGGTAGCTCTCAAAGTTGGGTTTGTTGGCACAGAAATAGACACTTAATCATTCCCATCCTACTAGTTTTGGTTTCAAGCTTCAGTACCCACCTCAGTTTCATAGCAGAAGCTACAAGACCAATTCCAACCAAGCTACCTGAAGCTGCCACAAGTGAAAATGCAGGTGGAGGGGAAAGGATTAACGCTCGTCAGATTGGGTCAAGGCCGCCGCAATGCATGAGGGCGTGCACTGGTTGTGGGCATTGTGTGGCGGTGCAGGTCCCGGTGGCTCCCCAAAGACACAACAATGGAGAAAAACGCAGTAGCAGCAGAGGTGCGGCTACTACTAGTACTAGGAAAACAAGCCCCAAGAGGCCTGCTTATTCTAGAGGAGGTGACGAGTTGTCGAATTACAAACCCATAAGCTGGAAATGCAAGTGTGGAGATTTGTTCTTCAATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

149

Amino Acids

16.13

Weight (kDa)

10.0

Isoelectric Point (pI)

62.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 66 - 148 1.7e-15 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 155
Acc36I ACCTGC 2 cut(s) 155, 250
AccB7I CCANNNNNTGG 1 cut(s) 408
AciI CCGC 4 cut(s) 209, 212, 254, 320
AcuI CTGAAG 2 cut(s) 67, 162
AfaI GTAC 2 cut(s) 88, 334
AfiI CCNNNNNNNGG 1 cut(s) 408
AgsI TTSAA 2 cut(s) 78, 439
AhdI GACNNNNNGTC 1 cut(s) 385
AhlI ACTAGT 2 cut(s) 64, 329
AluBI AGCT 6 cut(s) 9, 81, 113, 136, 146, 408
AluI AGCT 6 cut(s) 9, 81, 113, 136, 146, 408
Alw21I GWGCWC 1 cut(s) 233
Alw44I GTGCAC 1 cut(s) 229
AoxI GGCC 2 cut(s) 206, 356
ApaLI GTGCAC 1 cut(s) 229
ApeKI GCWGC 2 cut(s) 146, 309
AspS9I GGNCC 1 cut(s) 262
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 389
AvaII GGWCC 1 cut(s) 262
BaeGI GKGCMC 1 cut(s) 233
Bbv12I GWGCWC 1 cut(s) 233
BbvI GCAGC 2 cut(s) 133, 321
BccI CCATC 1 cut(s) 65
BcnI CCSGG 1 cut(s) 266
BcuI ACTAGT 2 cut(s) 64, 329
BfaI CTAG 4 cut(s) 65, 330, 336, 369
BfuAI ACCTGC 2 cut(s) 155, 250
BisI GCNGC 5 cut(s) 147, 209, 212, 310, 321
BlsI GCNGC 5 cut(s) 148, 210, 213, 311, 322
BmcAI AGTACT 1 cut(s) 334
Bme1390I CCNGG 1 cut(s) 266
Bme18I GGWCC 1 cut(s) 262
BmeRI GACNNNNNGTC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 2 cut(s) 264, 273
BmrFI CCNGG 1 cut(s) 266
BpuMI CCSGG 1 cut(s) 266
Bsc4I CCNNNNNNNGG 1 cut(s) 408
Bse1I ACTGG 1 cut(s) 238
Bse3DI GCAATG 1 cut(s) 221
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 408
BseMI GCAATG 1 cut(s) 221
BseMII CTCAG 1 cut(s) 109
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 1 cut(s) 387
BseSI GKGCMC 1 cut(s) 233
BseXI GCAGC 2 cut(s) 133, 321
BsgI GTGCAG 1 cut(s) 278
BshFI GGCC 2 cut(s) 208, 358
BsiHKAI GWGCWC 1 cut(s) 233
BsiSI CCGG 1 cut(s) 266
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 1 cut(s) 408
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 2 cut(s) 208, 358
Bsp1286I GDGCHC 1 cut(s) 233
BspACI CCGC 4 cut(s) 209, 212, 254, 320
BspANI GGCC 2 cut(s) 208, 358
BspCNI CTCAG 1 cut(s) 108
BspLI GGNNCC 2 cut(s) 264, 273
BspMI ACCTGC 2 cut(s) 155, 250
BsrDI GCAATG 1 cut(s) 221
BsrI ACTGG 1 cut(s) 238
BstC8I GCNNGC 2 cut(s) 229, 360
BstDEI CTNAG 1 cut(s) 95
BstF5I GGATG 1 cut(s) 57
BstMWI GCNNNNNNNGC 2 cut(s) 309, 414
BstSCI CCNGG 1 cut(s) 264
BstSLI GKGCMC 1 cut(s) 233
BstV1I GCAGC 2 cut(s) 133, 321
BsuRI GGCC 2 cut(s) 208, 358
BtsCI GGATG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 231
BveI ACCTGC 2 cut(s) 155, 250
Cac8I GCNNGC 2 cut(s) 229, 360
Cfr13I GGNCC 1 cut(s) 262
Csp6I GTAC 2 cut(s) 87, 333
CviAII CATG 1 cut(s) 220
CviQI GTAC 2 cut(s) 87, 333
DdeI CTNAG 1 cut(s) 95
DriI GACNNNNNGTC 1 cut(s) 385
Eam1105I GACNNNNNGTC 1 cut(s) 385
Eco147I AGGCCT 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 262
Eco57I CTGAAG 2 cut(s) 67, 162
EcoO109I RGGNCCY 1 cut(s) 262
EcoT22I ATGCAT 1 cut(s) 221
FaeI CATG 1 cut(s) 223
FaiI YATR 3 cut(s) 104, 221, 404
FaqI GGGAC 1 cut(s) 248
FatI CATG 1 cut(s) 219
Fnu4HI GCNGC 5 cut(s) 147, 209, 212, 310, 321
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 5 cut(s) 147, 209, 212, 310, 321
FspBI CTAG 4 cut(s) 65, 330, 336, 369
GluI GCNGC 5 cut(s) 147, 209, 212, 310, 321
HaeIII GGCC 2 cut(s) 208, 358
HapII CCGG 1 cut(s) 266
Hin1II CATG 1 cut(s) 223
HindIII AAGCTT 1 cut(s) 79
HpaII CCGG 1 cut(s) 266
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 1 cut(s) 195
Hpy188III TCNNGA 1 cut(s) 369
Hpy8I GTNNAC 1 cut(s) 231
HpyCH4V TGCA 5 cut(s) 164, 219, 231, 259, 417
HpyF10VI GCNNNNNNNGC 2 cut(s) 309, 414
HpyF3I CTNAG 1 cut(s) 95
Hsp92II CATG 1 cut(s) 223
LmnI GCTCC 1 cut(s) 277
LpnPI CCDG 7 cut(s) 150, 153, 219, 245, 279, 372, 394
Lsp1109I GCAGC 2 cut(s) 133, 321
MaeI CTAG 4 cut(s) 65, 330, 336, 369
MaeIII GTNAC 1 cut(s) 377
MboII GAAGA 1 cut(s) 427
MhlI GDGCHC 1 cut(s) 233
MluCI AATT 2 cut(s) 123, 391
MmeI TCCRAC 1 cut(s) 152
MnlI CCTC 7 cut(s) 104, 164, 216, 308, 348, 365, 368
Mph1103I ATGCAT 1 cut(s) 221
MseI TTAA 2 cut(s) 47, 183
MslI CAYNNNNRTG 1 cut(s) 288
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 1 cut(s) 266
MwoI GCNNNNNNNGC 2 cut(s) 309, 414
NciI CCSGG 1 cut(s) 266
NlaIII CATG 1 cut(s) 223
NlaIV GGNNCC 2 cut(s) 264, 273
NmuCI GTSAC 1 cut(s) 377
NsiI ATGCAT 1 cut(s) 221
PaqCI CACCTGC 1 cut(s) 155
PceI AGGCCT 1 cut(s) 358
PflMI CCANNNNNTGG 1 cut(s) 408
PkrI GCNGC 5 cut(s) 148, 210, 213, 311, 322
PpuMI RGGWCCY 1 cut(s) 262
Psp5II RGGWCCY 1 cut(s) 262
PspN4I GGNNCC 2 cut(s) 264, 273
PspPI GGNCC 1 cut(s) 262
PspPPI RGGWCCY 1 cut(s) 262
RsaI GTAC 2 cut(s) 88, 334
RsaNI GTAC 2 cut(s) 87, 333
RseI CAYNNNNRTG 1 cut(s) 288
SaqAI TTAA 2 cut(s) 47, 183
SatI GCNGC 5 cut(s) 147, 209, 212, 310, 321
Sau96I GGNCC 1 cut(s) 262
ScaI AGTACT 1 cut(s) 334
ScrFI CCNGG 1 cut(s) 266
SduI GDGCHC 1 cut(s) 233
SinI GGWCC 1 cut(s) 262
SmiMI CAYNNNNRTG 1 cut(s) 288
SpeI ACTAGT 2 cut(s) 64, 329
Sse9I AATT 2 cut(s) 123, 391
SseBI AGGCCT 1 cut(s) 358
SsiI CCGC 4 cut(s) 209, 212, 254, 320
SspMI CTAG 4 cut(s) 65, 330, 336, 369
StuI AGGCCT 1 cut(s) 358
StyD4I CCNGG 1 cut(s) 264
TaqI TCGA 1 cut(s) 389
TasI AATT 2 cut(s) 123, 391
TatI WGTACW 1 cut(s) 332
TauI GCSGC 3 cut(s) 211, 214, 323
Tru1I TTAA 2 cut(s) 47, 183
Tru9I TTAA 2 cut(s) 47, 183
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 1 cut(s) 377
TseI GCWGC 2 cut(s) 146, 309
Tsp45I GTSAC 1 cut(s) 377
TspDTI ATGAA 1 cut(s) 91
TspRI CASTG 1 cut(s) 238
Van91I CCANNNNNTGG 1 cut(s) 408
VneI GTGCAC 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 262
XbaI TCTAGA 1 cut(s) 368
XspI CTAG 4 cut(s) 65, 330, 336, 369
ZrmI AGTACT 1 cut(s) 334
Zsp2I ATGCAT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.