Rh6AG465600

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0004662
Physical Location & Seq
Forward (+)
64743784 .. 64746303
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG465600.1

Sequence Viewer

Length: 465 bp
ATGGCCAGCTCTCAAAACTGGGTTTGTTGGCACAGAAATAGACACATAATCATTCCTATCCTACTACTTTTGGTTTCAAGCTTCAGCACCCACTTCAGTTTCATAGCACAAGCTACTAGACCAATTCCAACCAAGCTACCTGAAGCAACTACAGGAGGCAATGTTTTGAACGAGGAAAATGCTGGTGGAGGGGAAAGGGTCAACGCTCGTCAAATTGGGTCAAGGCCGCCGCAATGCCAGAGGCGGTGCGGTGGTTGTGGGCATTGTGTGGCGGTGCAGGTCCCGGTGGTTCCCCAAGTTCAACAAAGCCACAACAATGGAGCAAAACGCAGCAGCAGCTCTGCTGCTTCTACTAGAAAAACAAGCCCCAAGAGCACTGCTTATTCCAGAGGAGGCGACGAGTTGTCGAATTACAAGCCCATAAGCTGGAGATGCAAGTGTGGAGATTTGTTCTTCAATCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

154

Amino Acids

16.76

Weight (kDa)

9.92

Isoelectric Point (pI)

57.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 72 - 154 4.3e-15 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 268
AccB7I CCANNNNNTGG 1 cut(s) 426
AciI CCGC 5 cut(s) 227, 230, 244, 249, 272
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 3 cut(s) 67, 79, 162
AfiI CCNNNNNNNGG 1 cut(s) 426
AgsI TTSAA 4 cut(s) 78, 169, 302, 457
AhdI GACNNNNNGTC 1 cut(s) 403
AluBI AGCT 6 cut(s) 9, 81, 113, 136, 339, 426
AluI AGCT 6 cut(s) 9, 81, 113, 136, 339, 426
Alw21I GWGCWC 1 cut(s) 377
AoxI GGCC 2 cut(s) 3, 224
ApeKI GCWGC 4 cut(s) 330, 333, 336, 344
AspS9I GGNCC 1 cut(s) 280
AsuC2I CCSGG 1 cut(s) 284
AvaII GGWCC 1 cut(s) 280
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 377
BbvI GCAGC 4 cut(s) 331, 342, 345, 348
BcgI CGANNNNNNTGC 2 cut(s) 161, 195
BcnI CCSGG 1 cut(s) 284
BfaI CTAG 2 cut(s) 117, 354
BfmI CTRYAG 1 cut(s) 150
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 6 cut(s) 227, 230, 331, 334, 337, 345
BlsI GCNGC 6 cut(s) 228, 231, 332, 335, 338, 346
Bme1390I CCNGG 1 cut(s) 284
Bme18I GGWCC 1 cut(s) 280
BmeRI GACNNNNNGTC 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 2 cut(s) 282, 291
BmrFI CCNGG 1 cut(s) 284
BmrI ACTGGG 1 cut(s) 28
BmsI GCATC 1 cut(s) 422
BmuI ACTGGG 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 448
BpuMI CCSGG 1 cut(s) 284
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bsc4I CCNNNNNNNGG 1 cut(s) 426
Bse1I ACTGG 1 cut(s) 23
Bse3DI GCAATG 2 cut(s) 166, 239
BseLI CCNNNNNNNGG 1 cut(s) 426
BseMI GCAATG 2 cut(s) 166, 239
BseNI ACTGG 1 cut(s) 23
BseRI GAGGAG 1 cut(s) 405
BseXI GCAGC 4 cut(s) 331, 342, 345, 348
BsgI GTGCAG 1 cut(s) 296
BshFI GGCC 2 cut(s) 5, 226
BsiHKAI GWGCWC 1 cut(s) 377
BsiSI CCGG 1 cut(s) 284
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 1 cut(s) 426
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 2 cut(s) 5, 226
Bsp1286I GDGCHC 1 cut(s) 377
BspACI CCGC 5 cut(s) 227, 230, 244, 249, 272
BspANI GGCC 2 cut(s) 5, 226
BspLI GGNNCC 2 cut(s) 282, 291
BspMI ACCTGC 1 cut(s) 268
BsrDI GCAATG 2 cut(s) 166, 239
BsrI ACTGG 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 7
BstMWI GCNNNNNNNGC 3 cut(s) 336, 372, 432
BstSCI CCNGG 1 cut(s) 282
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 4 cut(s) 331, 342, 345, 348
BstXI CCANNNNNNTGG 1 cut(s) 317
BsuRI GGCC 2 cut(s) 5, 226
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 1 cut(s) 375
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 280
DriI GACNNNNNGTC 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 3
Eam1105I GACNNNNNGTC 1 cut(s) 403
Eco47I GGWCC 1 cut(s) 280
Eco57I CTGAAG 3 cut(s) 67, 79, 162
EcoO109I RGGNCCY 1 cut(s) 280
FaiI YATR 3 cut(s) 47, 104, 422
FaqI GGGAC 1 cut(s) 266
Fnu4HI GCNGC 6 cut(s) 227, 230, 331, 334, 337, 345
Fsp4HI GCNGC 6 cut(s) 227, 230, 331, 334, 337, 345
FspBI CTAG 2 cut(s) 117, 354
GluI GCNGC 6 cut(s) 227, 230, 331, 334, 337, 345
GsuI CTGGAG 1 cut(s) 448
HaeIII GGCC 2 cut(s) 5, 226
HapII CCGG 1 cut(s) 284
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 79
HpaII CCGG 1 cut(s) 284
Hpy166II GTNNAC 1 cut(s) 202
Hpy188III TCNNGA 1 cut(s) 387
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 1 cut(s) 401
HpyCH4V TGCA 2 cut(s) 277, 435
HpyF10VI GCNNNNNNNGC 3 cut(s) 336, 372, 432
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 4 cut(s) 331, 342, 345, 348
LweI GCATC 1 cut(s) 422
MaeI CTAG 2 cut(s) 117, 354
MboII GAAGA 1 cut(s) 445
MhlI GDGCHC 1 cut(s) 377
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 123, 213, 409
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 152
MnlI CCTC 6 cut(s) 149, 166, 182, 234, 383, 386
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 315
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 284
MspR9I CCNGG 1 cut(s) 284
MwoI GCNNNNNNNGC 3 cut(s) 336, 372, 432
NciI CCSGG 1 cut(s) 284
NlaIV GGNNCC 2 cut(s) 282, 291
PflMI CCANNNNNTGG 1 cut(s) 426
PkrI GCNGC 6 cut(s) 228, 231, 332, 335, 338, 346
PpuMI RGGWCCY 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 280
PspN4I GGNNCC 2 cut(s) 282, 291
PspPI GGNCC 1 cut(s) 280
PspPPI RGGWCCY 1 cut(s) 280
RseI CAYNNNNRTG 1 cut(s) 315
SatI GCNGC 6 cut(s) 227, 230, 331, 334, 337, 345
Sau96I GGNCC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 284
SduI GDGCHC 1 cut(s) 377
SetI ASST 8 cut(s) 11, 83, 115, 138, 142, 282, 341, 428
SfaNI GCATC 1 cut(s) 422
SfcI CTRYAG 1 cut(s) 150
SinI GGWCC 1 cut(s) 280
SmiMI CAYNNNNRTG 1 cut(s) 315
Sse9I AATT 3 cut(s) 123, 213, 409
SsiI CCGC 5 cut(s) 227, 230, 244, 249, 272
SspMI CTAG 2 cut(s) 117, 354
StyD4I CCNGG 1 cut(s) 282
TaqI TCGA 1 cut(s) 407
TasI AATT 3 cut(s) 123, 213, 409
TauI GCSGC 2 cut(s) 229, 232
TscAI CASTG 1 cut(s) 382
TseI GCWGC 4 cut(s) 330, 333, 336, 344
TspDTI ATGAA 1 cut(s) 91
TspRI CASTG 1 cut(s) 382
Van91I CCANNNNNTGG 1 cut(s) 426
VpaK11BI GGWCC 1 cut(s) 280
XspI CTAG 2 cut(s) 117, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.