MD15G1088900.v1.1

Epidermal patterning factor proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004662
Physical Location & Seq
Reverse (-)
6191900 .. 6192676
777 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1088900.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGGCAGCTCTCAAAATTGCATCTTTTGCCAGAGAAATAGAAATATTTGCATTTCCATCCTCCTGTTCCTGGTTTCGAGCTCGACCCATCTGATTTTCATGGCTGAAGGTGGTAGACCAATTTCCAGCAAGCTAAGTGAGTTGGCTCGGGCAAGAGTTCTTCAGGAAAATAAAGAAGGGGTGGTGGTCAACGCTCGTCAAATAGGGTCAGTGCGGCCGAGCTGCGAGCGGAGGTGCAGCGTTTGCGGGCGTTGCGTGGCGGTTCAGGTTCCAGTTACTCCTCAAGTTGATAAAATCAGAAGCCATGGAAGCAGCTCCTCTGTTTCTTTTTCCAGAACTGCCCCAAAGAACGTAGCCTACTCCAGAGGAGATGACATTACAAATTATAAGCCAATGAGCTGGAAATGCAAGTGTGGGAATTTGCTCTTCAATCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

146

Amino Acids

15.93

Weight (kDa)

9.76

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 68 - 145 1.3e-12 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 387
AccBSI CCGCTC 1 cut(s) 229
AccI GTMKAC 1 cut(s) 115
AciI CCGC 4 cut(s) 214, 229, 246, 260
AcoI YGGCCR 1 cut(s) 215
AcsI RAATTY 1 cut(s) 418
AcuI CTGAAG 2 cut(s) 126, 146
AfiI CCNNNNNNNGG 1 cut(s) 70
AgsI TTSAA 1 cut(s) 430
AjnI CCWGG 1 cut(s) 69
AluBI AGCT 6 cut(s) 9, 81, 133, 222, 315, 399
AluI AGCT 6 cut(s) 9, 81, 133, 222, 315, 399
Alw21I GWGCWC 1 cut(s) 83
Ama87I CYCGRG 1 cut(s) 147
AoxI GGCC 1 cut(s) 215
ApeKI GCWGC 4 cut(s) 6, 222, 237, 312
ApoI RAATTY 1 cut(s) 418
AvaI CYCGRG 1 cut(s) 147
BanII GRGCYC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 4 cut(s) 18, 209, 249, 324
BccI CCATC 2 cut(s) 65, 96
BciT130I CCWGG 1 cut(s) 71
BisI GCNGC 5 cut(s) 7, 215, 223, 238, 313
BlsI GCNGC 5 cut(s) 8, 216, 224, 239, 314
Bme1390I CCNGG 1 cut(s) 71
BmeT110I CYCGRG 1 cut(s) 147
BmiI GGNNCC 1 cut(s) 270
BmrFI CCNGG 1 cut(s) 71
BmsI GCATC 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 346
BpuEI CTTGAG 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 304
Bsc4I CCNNNNNNNGG 1 cut(s) 70
Bse1I ACTGG 1 cut(s) 272
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 1 cut(s) 304
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 70
BseNI ACTGG 1 cut(s) 272
BseRI GAGGAG 3 cut(s) 270, 307, 381
BseX3I CGGCCG 1 cut(s) 215
BseXI GCAGC 4 cut(s) 18, 209, 249, 324
BsgI GTGCAG 1 cut(s) 256
Bsh1285I CGRYCG 1 cut(s) 218
BshFI GGCC 1 cut(s) 217
BsiEI CGRYCG 1 cut(s) 218
BsiHKAI GWGCWC 1 cut(s) 83
BsiHKCI CYCGRG 1 cut(s) 147
BslI CCNNNNNNNGG 1 cut(s) 70
BsnI GGCC 1 cut(s) 217
BsoBI CYCGRG 1 cut(s) 147
Bsp1286I GDGCHC 1 cut(s) 83
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 4 cut(s) 214, 229, 246, 260
BspANI GGCC 1 cut(s) 217
BspLI GGNNCC 1 cut(s) 270
BspQI GCTCTTC 1 cut(s) 431
BsrBI CCGCTC 1 cut(s) 229
BsrI ACTGG 1 cut(s) 272
BssECI CCNNGG 1 cut(s) 304
BssT1I CCWWGG 1 cut(s) 304
Bst2UI CCWGG 1 cut(s) 71
Bst6I CTCTTC 1 cut(s) 431
BstAPI GCANNNNNTGC 2 cut(s) 27, 243
BstC8I GCNNGC 3 cut(s) 131, 227, 248
BstDEI CTNAG 1 cut(s) 134
BstDSI CCRYGG 1 cut(s) 304
BstF5I GGATG 1 cut(s) 57
BstMCI CGRYCG 1 cut(s) 218
BstMWI GCNNNNNNNGC 5 cut(s) 27, 243, 252, 309, 405
BstNI CCWGG 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 69
BstV1I GCAGC 4 cut(s) 18, 209, 249, 324
BstXI CCANNNNNNTGG 1 cut(s) 399
BstZI CGGCCG 1 cut(s) 215
BsuRI GGCC 1 cut(s) 217
BtgI CCRYGG 1 cut(s) 304
BtsCI GGATG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 3 cut(s) 131, 227, 248
CviAII CATG 2 cut(s) 100, 305
DdeI CTNAG 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 215
EagI CGGCCG 1 cut(s) 215
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
Ecl136II GAGCTC 1 cut(s) 81
EclXI CGGCCG 1 cut(s) 215
Eco130I CCWWGG 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 83
Eco52I CGGCCG 1 cut(s) 215
Eco53kI GAGCTC 1 cut(s) 81
Eco57I CTGAAG 2 cut(s) 126, 146
Eco88I CYCGRG 1 cut(s) 147
EcoICRI GAGCTC 1 cut(s) 81
EcoRII CCWGG 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 2 cut(s) 103, 308
FaiI YATR 3 cut(s) 101, 306, 387
FatI CATG 2 cut(s) 99, 304
FauI CCCGC 1 cut(s) 239
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 5 cut(s) 7, 215, 223, 238, 313
FokI GGATG 1 cut(s) 44
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 5 cut(s) 7, 215, 223, 238, 313
GluI GCNGC 5 cut(s) 7, 215, 223, 238, 313
GsuI CTGGAG 1 cut(s) 346
HaeIII GGCC 1 cut(s) 217
Hin1II CATG 2 cut(s) 103, 308
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
Hpy166II GTNNAC 2 cut(s) 116, 190
Hpy188I TCNGA 2 cut(s) 93, 299
Hpy188III TCNNGA 3 cut(s) 164, 333, 363
Hpy8I GTNNAC 2 cut(s) 116, 190
HpyAV CCTTC 2 cut(s) 101, 170
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 4 cut(s) 21, 51, 237, 408
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 243, 252, 309, 405
HpyF3I CTNAG 1 cut(s) 134
HpySE526I ACGT 1 cut(s) 351
Hsp92II CATG 2 cut(s) 103, 308
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 4 cut(s) 18, 209, 249, 324
LweI GCATC 1 cut(s) 30
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 1 cut(s) 274
MbiI CCGCTC 1 cut(s) 229
MboII GAAGA 2 cut(s) 152, 418
MhlI GDGCHC 1 cut(s) 83
MluCI AATT 4 cut(s) 16, 120, 382, 418
MnlI CCTC 5 cut(s) 71, 225, 291, 328, 359
MseI TTAA 1 cut(s) 436
MspR9I CCNGG 1 cut(s) 71
MvaI CCWGG 1 cut(s) 71
MwoI GCNNNNNNNGC 5 cut(s) 27, 243, 252, 309, 405
NcoI CCATGG 1 cut(s) 304
NlaIII CATG 2 cut(s) 103, 308
NlaIV GGNNCC 1 cut(s) 270
NmeAIII GCCGAG 1 cut(s) 243
PciSI GCTCTTC 1 cut(s) 431
PkrI GCNGC 5 cut(s) 8, 216, 224, 239, 314
PsiI TTATAA 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 69
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 1 cut(s) 270
PsrI GAACNNNNNNTAC 2 cut(s) 341, 373
SacI GAGCTC 1 cut(s) 83
SapI GCTCTTC 1 cut(s) 431
SaqAI TTAA 1 cut(s) 436
SatI GCNGC 5 cut(s) 7, 215, 223, 238, 313
ScrFI CCNGG 1 cut(s) 71
SduI GDGCHC 1 cut(s) 83
SfaNI GCATC 1 cut(s) 30
SmlI CTYRAG 1 cut(s) 282
SmoI CTYRAG 1 cut(s) 282
Sse9I AATT 4 cut(s) 16, 120, 382, 418
SsiI CCGC 4 cut(s) 214, 229, 246, 260
SspI AATATT 1 cut(s) 46
SstI GAGCTC 1 cut(s) 83
StyD4I CCNGG 1 cut(s) 69
StyI CCWWGG 1 cut(s) 304
TaiI ACGT 1 cut(s) 354
TaqI TCGA 2 cut(s) 77, 83
TasI AATT 4 cut(s) 16, 120, 382, 418
TauI GCSGC 1 cut(s) 217
Tru1I TTAA 1 cut(s) 436
Tru9I TTAA 1 cut(s) 436
TscAI CASTG 1 cut(s) 216
TseI GCWGC 4 cut(s) 6, 222, 237, 312
TspDTI ATGAA 1 cut(s) 88
TspRI CASTG 1 cut(s) 216
XapI RAATTY 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.