MD02G1193400.v1.1

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004662
Physical Location & Seq
Forward (+)
18404735 .. 18405488
754 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1193400.v1.1.491

Sequence Viewer

Length: 387 bp
ATGGGTTGCTTCCACTTTTACTCAATTTCCCTTATGTTTCTCTTGATTCTGTGCCCAACCCAGTTCAGATTTTGTACCAAATTGGCTGAAAGCAGAGCAATTCACAACAAAGTTGTCATTTCCCAGGCAGGGAATGAAGAGAGTAAAGTGAATCTGAGAGGTCAGATAGGTTCGAGGCCTCCAAGGTGTGAGAGAAGGTGCAGCTCCTGTGGGCACTGCGAGGCAATTCAAATGCCTGAAAATCCCCAAGCAAATGCAATTACAGGCAGCAAAAACTCTTCCAAAGTTGTATCCAGCATTGCTTATGCCAAAGGTGATGAGAACTCCAGCAACTACAAGCCCATGAGTTGGAAGTGCAAGTGTGGCAGCTCCATCTTCAATCCCTAA

Protein Analysis

129

Amino Acids

14.14

Weight (kDa)

9.18

Isoelectric Point (pI)

53.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 56 - 128 1.8e-17 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 348
AfaI GTAC 1 cut(s) 76
AfiI CCNNNNNNNGG 2 cut(s) 129, 348
AgsI TTSAA 2 cut(s) 230, 379
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 2 cut(s) 204, 369
AluI AGCT 2 cut(s) 204, 369
AlwNI CAGNNNCTG 1 cut(s) 207
AoxI GGCC 1 cut(s) 176
ApeKI GCWGC 3 cut(s) 201, 267, 366
AsuHPI GGTGA 1 cut(s) 326
BaeGI GKGCMC 2 cut(s) 56, 216
BbvI GCAGC 3 cut(s) 213, 279, 378
BccI CCATC 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 125
BciVI GTATCC 1 cut(s) 301
BfuI GTATCC 1 cut(s) 301
BisI GCNGC 3 cut(s) 202, 268, 367
BlsI GCNGC 3 cut(s) 203, 269, 368
Bme1390I CCNGG 1 cut(s) 125
BmrFI CCNGG 1 cut(s) 125
BmrI ACTGGG 1 cut(s) 55
BmuI ACTGGG 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 310
BsaJI CCNNGG 2 cut(s) 123, 182
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 348
Bse1I ACTGG 1 cut(s) 61
Bse3DI GCAATG 1 cut(s) 297
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 2 cut(s) 123, 182
BseLI CCNNNNNNNGG 2 cut(s) 129, 348
BseMI GCAATG 1 cut(s) 297
BseMII CTCAG 1 cut(s) 146
BseNI ACTGG 1 cut(s) 61
BseSI GKGCMC 2 cut(s) 56, 216
BseXI GCAGC 3 cut(s) 213, 279, 378
BsgI GTGCAG 1 cut(s) 220
BshFI GGCC 1 cut(s) 178
BslI CCNNNNNNNGG 2 cut(s) 129, 348
BsnI GGCC 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 56, 216
BspANI GGCC 1 cut(s) 178
BspCNI CTCAG 1 cut(s) 147
BsrDI GCAATG 1 cut(s) 297
BsrI ACTGG 1 cut(s) 61
BssECI CCNNGG 2 cut(s) 123, 182
BssT1I CCWWGG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 125
Bst6I CTCTTC 2 cut(s) 132, 283
BstDEI CTNAG 1 cut(s) 155
BstMWI GCNNNNNNNGC 1 cut(s) 363
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 123
BstSLI GKGCMC 2 cut(s) 56, 216
BstV1I GCAGC 3 cut(s) 213, 279, 378
BsuI GTATCC 1 cut(s) 301
BsuRI GGCC 1 cut(s) 178
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 1 cut(s) 214
CaiI CAGNNNCTG 1 cut(s) 207
Csp6I GTAC 1 cut(s) 75
CviAII CATG 1 cut(s) 343
CviJI RGCY 5 cut(s) 86, 178, 204, 340, 369
CviKI_1 RGCY 5 cut(s) 86, 178, 204, 340, 369
CviQI GTAC 1 cut(s) 75
DdeI CTNAG 1 cut(s) 155
Eam1104I CTCTTC 2 cut(s) 132, 283
EarI CTCTTC 2 cut(s) 132, 283
Eco130I CCWWGG 1 cut(s) 182
Eco147I AGGCCT 1 cut(s) 178
EcoRII CCWGG 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 182
ErhI CCWWGG 1 cut(s) 182
FaeI CATG 1 cut(s) 346
FaiI YATR 3 cut(s) 35, 306, 344
FatI CATG 1 cut(s) 342
Fnu4HI GCNGC 3 cut(s) 202, 268, 367
Fsp4HI GCNGC 3 cut(s) 202, 268, 367
GluI GCNGC 3 cut(s) 202, 268, 367
GsuI CTGGAG 1 cut(s) 310
HaeIII GGCC 1 cut(s) 178
Hin1II CATG 1 cut(s) 346
HinfI GANTC 2 cut(s) 46, 151
HphI GGTGA 1 cut(s) 326
Hpy188I TCNGA 3 cut(s) 68, 156, 165
Hpy188III TCNNGA 1 cut(s) 43
HpyAV CCTTC 1 cut(s) 189
HpyCH4V TGCA 3 cut(s) 201, 257, 357
HpyF10VI GCNNNNNNNGC 1 cut(s) 363
HpyF3I CTNAG 1 cut(s) 155
Hsp92II CATG 1 cut(s) 346
LmnI GCTCC 2 cut(s) 209, 374
LpnPI CCDG 9 cut(s) 74, 110, 114, 137, 220, 249, 249, 307, 340
Lsp1109I GCAGC 3 cut(s) 213, 279, 378
MboII GAAGA 3 cut(s) 149, 270, 367
MhlI GDGCHC 2 cut(s) 56, 216
MluCI AATT 5 cut(s) 24, 80, 99, 225, 258
MmeI TCCRAC 1 cut(s) 329
MnlI CCTC 4 cut(s) 152, 168, 189, 214
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 1 cut(s) 363
NlaIII CATG 1 cut(s) 346
PceI AGGCCT 1 cut(s) 178
PfeI GAWTC 2 cut(s) 46, 151
PflMI CCANNNNNTGG 1 cut(s) 348
PkrI GCNGC 3 cut(s) 203, 269, 368
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PstNI CAGNNNCTG 1 cut(s) 207
RsaI GTAC 1 cut(s) 76
RsaNI GTAC 1 cut(s) 75
SatI GCNGC 3 cut(s) 202, 268, 367
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 2 cut(s) 56, 216
SetI ASST 7 cut(s) 163, 172, 188, 200, 206, 316, 371
Sse9I AATT 5 cut(s) 24, 80, 99, 225, 258
SseBI AGGCCT 1 cut(s) 178
StuI AGGCCT 1 cut(s) 178
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 1 cut(s) 182
TaqI TCGA 1 cut(s) 173
TasI AATT 5 cut(s) 24, 80, 99, 225, 258
TfiI GAWTC 2 cut(s) 46, 151
TscAI CASTG 1 cut(s) 221
TseI GCWGC 3 cut(s) 201, 267, 366
TspDTI ATGAA 1 cut(s) 150
TspRI CASTG 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.