Rw6G040580

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004662
Physical Location & Seq
Forward (+)
64322883 .. 64323832
950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040580.1

Sequence Viewer

Length: 456 bp
ATGGCCAGCTCTCAAAACTGGGTTTGTTGGCACAGAAATAGACACATAATCATTCCTATCCTACTACTTTTGGTTTCAAGCTTCAGTACCCACTTCAGTTTCATAGCACAAGCTACTAGACCAATTCCAACCAAGCTACCTGAAGCAACTACAGGAGGCAATGTTTTAAACGAGGAAAATGCTGGTGGAGGGGAAAGGGTCAACGCTCGTCAAATTGGGTCAAGGCCGCCGCAATGCCAGAGGCGGTGCGGTGGTTGTGGGCATTGCGTGGCGGTGCAGGTCCCGGTGGCTCCCCAAGTTCAACAAAGCCACAACAATGGAGCAAAACGCTCTGCTGCTTCTACTAGAAAAACAAGCCCCAAGAGCACTGCTTATTCCAGAGGAGGCGACGAGTTGTCGAATTACAAGCCCATAAGCTGGAGATGCAAGTGTGGAGATTTGTTCTTCAATCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

151

Amino Acids

16.47

Weight (kDa)

9.92

Isoelectric Point (pI)

54.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 72 - 151 7.8e-16 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 268
AccB7I CCANNNNNTGG 1 cut(s) 417
AciI CCGC 5 cut(s) 227, 230, 244, 249, 272
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 3 cut(s) 67, 79, 162
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 1 cut(s) 417
AgsI TTSAA 3 cut(s) 78, 302, 448
AhdI GACNNNNNGTC 1 cut(s) 394
AluBI AGCT 5 cut(s) 9, 81, 113, 136, 417
AluI AGCT 5 cut(s) 9, 81, 113, 136, 417
Alw21I GWGCWC 1 cut(s) 368
AoxI GGCC 2 cut(s) 3, 224
ApeKI GCWGC 1 cut(s) 335
AspS9I GGNCC 1 cut(s) 280
AsuC2I CCSGG 1 cut(s) 284
AvaII GGWCC 1 cut(s) 280
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 368
BbvI GCAGC 1 cut(s) 322
BcgI CGANNNNNNTGC 2 cut(s) 161, 195
BcnI CCSGG 1 cut(s) 284
BfaI CTAG 2 cut(s) 117, 345
BfmI CTRYAG 1 cut(s) 150
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 3 cut(s) 227, 230, 336
BlsI GCNGC 3 cut(s) 228, 231, 337
Bme1390I CCNGG 1 cut(s) 284
Bme18I GGWCC 1 cut(s) 280
BmeRI GACNNNNNGTC 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 2 cut(s) 282, 291
BmrFI CCNGG 1 cut(s) 284
BmrI ACTGGG 1 cut(s) 28
BmsI GCATC 1 cut(s) 413
BmuI ACTGGG 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 439
BpuMI CCSGG 1 cut(s) 284
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bsc4I CCNNNNNNNGG 1 cut(s) 417
Bse1I ACTGG 1 cut(s) 23
Bse3DI GCAATG 3 cut(s) 166, 239, 262
BseLI CCNNNNNNNGG 1 cut(s) 417
BseMI GCAATG 3 cut(s) 166, 239, 262
BseNI ACTGG 1 cut(s) 23
BseRI GAGGAG 1 cut(s) 396
BseXI GCAGC 1 cut(s) 322
BsgI GTGCAG 1 cut(s) 296
BshFI GGCC 2 cut(s) 5, 226
BsiHKAI GWGCWC 1 cut(s) 368
BsiSI CCGG 1 cut(s) 284
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 2 cut(s) 5, 226
Bsp1286I GDGCHC 1 cut(s) 368
BspACI CCGC 5 cut(s) 227, 230, 244, 249, 272
BspANI GGCC 2 cut(s) 5, 226
BspLI GGNNCC 2 cut(s) 282, 291
BspMI ACCTGC 1 cut(s) 268
BsrDI GCAATG 3 cut(s) 166, 239, 262
BsrI ACTGG 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 7
BstMWI GCNNNNNNNGC 2 cut(s) 363, 423
BstSCI CCNGG 1 cut(s) 282
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 1 cut(s) 322
BstXI CCANNNNNNTGG 1 cut(s) 317
BsuRI GGCC 2 cut(s) 5, 226
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 1 cut(s) 366
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 280
Csp6I GTAC 1 cut(s) 87
CviQI GTAC 1 cut(s) 87
DraI TTTAAA 1 cut(s) 168
DriI GACNNNNNGTC 1 cut(s) 394
EaeI YGGCCR 1 cut(s) 3
Eam1105I GACNNNNNGTC 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 280
Eco57I CTGAAG 3 cut(s) 67, 79, 162
EcoO109I RGGNCCY 1 cut(s) 280
FaiI YATR 3 cut(s) 47, 104, 413
FaqI GGGAC 1 cut(s) 266
Fnu4HI GCNGC 3 cut(s) 227, 230, 336
Fsp4HI GCNGC 3 cut(s) 227, 230, 336
FspBI CTAG 2 cut(s) 117, 345
GluI GCNGC 3 cut(s) 227, 230, 336
GsuI CTGGAG 1 cut(s) 439
HaeIII GGCC 2 cut(s) 5, 226
HapII CCGG 1 cut(s) 284
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 79
HpaII CCGG 1 cut(s) 284
Hpy166II GTNNAC 1 cut(s) 202
Hpy188III TCNNGA 1 cut(s) 378
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 1 cut(s) 392
HpyCH4V TGCA 2 cut(s) 277, 426
HpyF10VI GCNNNNNNNGC 2 cut(s) 363, 423
LmnI GCTCC 2 cut(s) 295, 320
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 1 cut(s) 413
MaeI CTAG 2 cut(s) 117, 345
MboII GAAGA 1 cut(s) 436
MhlI GDGCHC 1 cut(s) 368
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 123, 213, 400
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 152
MnlI CCTC 6 cut(s) 149, 166, 182, 234, 374, 377
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 167
MslI CAYNNNNRTG 1 cut(s) 315
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 284
MspR9I CCNGG 1 cut(s) 284
MwoI GCNNNNNNNGC 2 cut(s) 363, 423
NciI CCSGG 1 cut(s) 284
NlaIV GGNNCC 2 cut(s) 282, 291
PflMI CCANNNNNTGG 1 cut(s) 417
PkrI GCNGC 3 cut(s) 228, 231, 337
PpuMI RGGWCCY 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 280
PspN4I GGNNCC 2 cut(s) 282, 291
PspPI GGNCC 1 cut(s) 280
PspPPI RGGWCCY 1 cut(s) 280
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
RseI CAYNNNNRTG 1 cut(s) 315
SaqAI TTAA 1 cut(s) 167
SatI GCNGC 3 cut(s) 227, 230, 336
Sau96I GGNCC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 284
SduI GDGCHC 1 cut(s) 368
SetI ASST 7 cut(s) 11, 83, 115, 138, 142, 282, 419
SfaNI GCATC 1 cut(s) 413
SfcI CTRYAG 1 cut(s) 150
SinI GGWCC 1 cut(s) 280
SmiMI CAYNNNNRTG 1 cut(s) 315
Sse9I AATT 3 cut(s) 123, 213, 400
SsiI CCGC 5 cut(s) 227, 230, 244, 249, 272
SspMI CTAG 2 cut(s) 117, 345
StyD4I CCNGG 1 cut(s) 282
TaqI TCGA 1 cut(s) 398
TasI AATT 3 cut(s) 123, 213, 400
TauI GCSGC 2 cut(s) 229, 232
Tru1I TTAA 1 cut(s) 167
Tru9I TTAA 1 cut(s) 167
TscAI CASTG 1 cut(s) 373
TseI GCWGC 1 cut(s) 335
TspDTI ATGAA 1 cut(s) 91
TspRI CASTG 1 cut(s) 373
Van91I CCANNNNNTGG 1 cut(s) 417
VpaK11BI GGWCC 1 cut(s) 280
XspI CTAG 2 cut(s) 117, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.