MD08G1108300.v1.1

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004662
Physical Location & Seq
Reverse (-)
9543448 .. 9544106
659 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1108300.v1.1.491

Sequence Viewer

Length: 423 bp
ATGGGCAGCTCTCAAGATTTCATCTTTTGCCACAGAAATAGAATTCTTTGCGTTTCCATCCTCCTGTTCTTGGTTTCGAGCTCGGCCCATCTGATATTCCTGGCTGAAGGTGGTAGAACAATTTCAGGCAAGCAAACTGAGGCTGCTCCGGCGAGAGTTCTAGAGGAAAATAGAGCAGGAGTGGCCGTCAACGCACGTCAAATAGGATCAGTGCCGCCGCTGTGCGAGCGGATTTGCAGCGCTTGCGGGCGTTGCGTGGCGGTTCAGGTTCCAGTTGCTCCTCAAGGCAATAAAATGAGAAGCCATGGCGGCAGCTCCTCTACTTCTCCCATGAATGTAGCTTACTCCAGAGGAGATGACATTACAAATTATAAGCCAATGAGCTGGAAATGCAAGTGTGGGAATTTGCTCTTCAATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

141

Amino Acids

15.01

Weight (kDa)

9.22

Isoelectric Point (pI)

53.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 68 - 140 3e-14 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AccBSI CCGCTC 1 cut(s) 229
AciI CCGC 6 cut(s) 215, 218, 229, 246, 260, 309
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 2 cut(s) 42, 403
AcuI CTGAAG 1 cut(s) 126
AfeI AGCGCT 1 cut(s) 241
AfiI CCNNNNNNNGG 1 cut(s) 70
AgsI TTSAA 1 cut(s) 415
AjiI CACGTC 1 cut(s) 197
AjnI CCWGG 1 cut(s) 99
AluBI AGCT 5 cut(s) 9, 81, 315, 341, 384
AluI AGCT 5 cut(s) 9, 81, 315, 341, 384
Alw21I GWGCWC 1 cut(s) 83
AlwI GGATC 1 cut(s) 214
Aor51HI AGCGCT 1 cut(s) 241
AoxI GGCC 2 cut(s) 84, 183
ApeKI GCWGC 4 cut(s) 6, 143, 237, 312
ApoI RAATTY 2 cut(s) 42, 403
Asp700I GAANNNNTTC 1 cut(s) 121
AspLEI GCGC 1 cut(s) 242
AspS9I GGNCC 1 cut(s) 85
BanII GRGCYC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 4 cut(s) 18, 130, 249, 324
BccI CCATC 2 cut(s) 65, 96
BceAI ACGGC 1 cut(s) 170
BciT130I CCWGG 1 cut(s) 101
BfaI CTAG 1 cut(s) 161
BfoI RGCGCY 1 cut(s) 243
BglI GCCNNNNNGGC 1 cut(s) 309
BisI GCNGC 7 cut(s) 7, 144, 215, 218, 238, 310, 313
BlsI GCNGC 7 cut(s) 8, 145, 216, 219, 239, 311, 314
Bme1390I CCNGG 1 cut(s) 101
BmgBI CACGTC 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 85
BmiI GGNNCC 1 cut(s) 270
BmrFI CCNGG 1 cut(s) 101
BpmI CTGGAG 1 cut(s) 331
BpuEI CTTGAG 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 304
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 1 cut(s) 70
Bse1I ACTGG 1 cut(s) 272
BseBI CCWGG 1 cut(s) 101
BseDI CCNNGG 1 cut(s) 304
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 70
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 1 cut(s) 272
BseRI GAGGAG 3 cut(s) 270, 307, 366
BseXI GCAGC 4 cut(s) 18, 130, 249, 324
BshFI GGCC 2 cut(s) 86, 185
BsiHKAI GWGCWC 1 cut(s) 83
BsiSI CCGG 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 70
BsnI GGCC 2 cut(s) 86, 185
Bsp1286I GDGCHC 1 cut(s) 83
Bsp143I GATC 1 cut(s) 206
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 6 cut(s) 215, 218, 229, 246, 260, 309
BspANI GGCC 2 cut(s) 86, 185
BspCNI CTCAG 1 cut(s) 130
BspLI GGNNCC 1 cut(s) 270
BspPI GGATC 1 cut(s) 214
BspQI GCTCTTC 1 cut(s) 416
BsrBI CCGCTC 1 cut(s) 229
BsrI ACTGG 1 cut(s) 272
BssECI CCNNGG 1 cut(s) 304
BssMI GATC 1 cut(s) 206
BssT1I CCWWGG 1 cut(s) 304
Bst2UI CCWGG 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 243
BstC8I GCNNGC 4 cut(s) 131, 227, 244, 248
BstDEI CTNAG 1 cut(s) 138
BstDSI CCRYGG 1 cut(s) 304
BstF5I GGATG 1 cut(s) 57
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 1 cut(s) 209
BstMBI GATC 1 cut(s) 206
BstMWI GCNNNNNNNGC 8 cut(s) 149, 182, 191, 226, 243, 252, 309, 390
BstNI CCWGG 1 cut(s) 101
BstSCI CCNGG 1 cut(s) 99
BstV1I GCAGC 4 cut(s) 18, 130, 249, 324
BstXI CCANNNNNNTGG 1 cut(s) 384
BsuRI GGCC 2 cut(s) 86, 185
BtgI CCRYGG 1 cut(s) 304
BtrI CACGTC 1 cut(s) 197
BtsCI GGATG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 4 cut(s) 131, 227, 244, 248
CfoI GCGC 1 cut(s) 242
Cfr13I GGNCC 1 cut(s) 85
CviAII CATG 2 cut(s) 305, 331
DdeI CTNAG 1 cut(s) 138
DpnI GATC 1 cut(s) 208
DpnII GATC 1 cut(s) 206
EaeI YGGCCR 1 cut(s) 183
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
Ecl136II GAGCTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 83
Eco47III AGCGCT 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 81
Eco57I CTGAAG 1 cut(s) 126
EcoICRI GAGCTC 1 cut(s) 81
EcoRI GAATTC 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 99
EcoT14I CCWWGG 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 2 cut(s) 308, 334
FaiI YATR 3 cut(s) 306, 332, 372
FatI CATG 2 cut(s) 304, 330
FauI CCCGC 1 cut(s) 239
Fnu4HI GCNGC 7 cut(s) 7, 144, 215, 218, 238, 310, 313
FokI GGATG 1 cut(s) 44
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 7 cut(s) 7, 144, 215, 218, 238, 310, 313
FspBI CTAG 1 cut(s) 161
GlaI GCGC 1 cut(s) 241
GluI GCNGC 7 cut(s) 7, 144, 215, 218, 238, 310, 313
GsuI CTGGAG 1 cut(s) 331
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 2 cut(s) 86, 185
HapII CCGG 1 cut(s) 149
HhaI GCGC 1 cut(s) 242
Hin1II CATG 2 cut(s) 308, 334
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HpaII CCGG 1 cut(s) 149
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 1 cut(s) 93
Hpy188III TCNNGA 3 cut(s) 14, 161, 348
Hpy8I GTNNAC 1 cut(s) 190
HpyAV CCTTC 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 196
HpyCH4V TGCA 2 cut(s) 237, 393
HpyF10VI GCNNNNNNNGC 8 cut(s) 149, 182, 191, 226, 243, 252, 309, 390
HpyF3I CTNAG 1 cut(s) 138
HpySE526I ACGT 1 cut(s) 196
Hsp92II CATG 2 cut(s) 308, 334
HspAI GCGC 1 cut(s) 240
Kzo9I GATC 1 cut(s) 206
LguI GCTCTTC 1 cut(s) 416
LmnI GCTCC 3 cut(s) 151, 283, 320
Lsp1109I GCAGC 4 cut(s) 18, 130, 249, 324
MaeI CTAG 1 cut(s) 161
MaeII ACGT 1 cut(s) 196
MalI GATC 1 cut(s) 208
MbiI CCGCTC 1 cut(s) 229
MboI GATC 1 cut(s) 206
MboII GAAGA 1 cut(s) 403
MhlI GDGCHC 1 cut(s) 83
MluCI AATT 4 cut(s) 42, 120, 367, 403
MnlI CCTC 6 cut(s) 71, 133, 157, 291, 328, 344
MroXI GAANNNNTTC 1 cut(s) 121
MspA1I CMGCKG 1 cut(s) 220
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 1 cut(s) 101
MvaI CCWGG 1 cut(s) 101
MwoI GCNNNNNNNGC 8 cut(s) 149, 182, 191, 226, 243, 252, 309, 390
NcoI CCATGG 1 cut(s) 304
NdeII GATC 1 cut(s) 206
NlaIII CATG 2 cut(s) 308, 334
NlaIV GGNNCC 1 cut(s) 270
NmeAIII GCCGAG 1 cut(s) 62
PciSI GCTCTTC 1 cut(s) 416
PdmI GAANNNNTTC 1 cut(s) 121
PkrI GCNGC 7 cut(s) 8, 145, 216, 219, 239, 311, 314
PsiI TTATAA 1 cut(s) 372
Psp124BI GAGCTC 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 1 cut(s) 270
PspPI GGNCC 1 cut(s) 85
SacI GAGCTC 1 cut(s) 83
SapI GCTCTTC 1 cut(s) 416
SatI GCNGC 7 cut(s) 7, 144, 215, 218, 238, 310, 313
Sau3AI GATC 1 cut(s) 206
Sau96I GGNCC 1 cut(s) 85
ScrFI CCNGG 1 cut(s) 101
SduI GDGCHC 1 cut(s) 83
SetI ASST 8 cut(s) 11, 83, 112, 199, 270, 317, 343, 386
SmlI CTYRAG 2 cut(s) 12, 282
SmoI CTYRAG 2 cut(s) 12, 282
Sse9I AATT 4 cut(s) 42, 120, 367, 403
SsiI CCGC 6 cut(s) 215, 218, 229, 246, 260, 309
SspMI CTAG 1 cut(s) 161
SstI GAGCTC 1 cut(s) 83
StyD4I CCNGG 1 cut(s) 99
StyI CCWWGG 1 cut(s) 304
TaiI ACGT 1 cut(s) 199
TaqI TCGA 1 cut(s) 77
TasI AATT 4 cut(s) 42, 120, 367, 403
TauI GCSGC 3 cut(s) 217, 220, 312
TscAI CASTG 1 cut(s) 216
TseI GCWGC 4 cut(s) 6, 143, 237, 312
TspDTI ATGAA 2 cut(s) 10, 347
TspRI CASTG 1 cut(s) 216
XapI RAATTY 2 cut(s) 42, 403
XbaI TCTAGA 1 cut(s) 160
XmnI GAANNNNTTC 1 cut(s) 121
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.