Rh6BG435200

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004662
Physical Location & Seq
Reverse (-)
65535738 .. 65537205
1468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG435200.1

Sequence Viewer

Length: 462 bp
ATGGCCAGCTCTCAAAACTGGGTTTGTTGGCACAGAAATAGACACATAATCATTCCCATCCTACTAGTTTTGGTTTCAAGCTTCAGTACCCACTTCAGTTTCATAGCACAAGCTACTAGACCAATTCCAACCAAGCTACCTGAAGCAACTACAGGAGGCAATGTTTTGAACGAGGAAAATGCTGGTGGAAAGGAAAGGGTCAACGCTCGTCAAATTGGGTCAAGGCCGCCGCAATGCCAGAGGCGGTGCGGTGGTTGCGGGCATTGTGTGGCGGTGCAGGTCCCGGTGGCTCCCCAAGTTCAACAAAGCCACAACAATGGAGCAAAACGCAGCAGCTCTGCTGCTTCTACTAGAAAAACAAGCCCCAAGAGCACTGCTTATTCCAGAGGAGGCGACGAGTTGTCGAATTACAAGCCCATAAGCTGGAGATGCAAGTGTGGAGATTTGTTCTTCAATCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

153

Amino Acids

16.7

Weight (kDa)

10.0

Isoelectric Point (pI)

55.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 72 - 153 1.1e-15 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 268
AccB7I CCANNNNNTGG 1 cut(s) 423
AciI CCGC 6 cut(s) 227, 230, 244, 249, 258, 272
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 3 cut(s) 67, 79, 162
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 1 cut(s) 423
AgsI TTSAA 4 cut(s) 78, 169, 302, 454
AhdI GACNNNNNGTC 1 cut(s) 400
AhlI ACTAGT 1 cut(s) 64
AluBI AGCT 6 cut(s) 9, 81, 113, 136, 336, 423
AluI AGCT 6 cut(s) 9, 81, 113, 136, 336, 423
Alw21I GWGCWC 1 cut(s) 374
AoxI GGCC 2 cut(s) 3, 224
ApeKI GCWGC 3 cut(s) 330, 333, 341
AspS9I GGNCC 1 cut(s) 280
AsuC2I CCSGG 1 cut(s) 284
AvaII GGWCC 1 cut(s) 280
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 374
BbvI GCAGC 3 cut(s) 328, 342, 345
BccI CCATC 1 cut(s) 65
BcgI CGANNNNNNTGC 2 cut(s) 161, 195
BcnI CCSGG 1 cut(s) 284
BcuI ACTAGT 1 cut(s) 64
BfaI CTAG 3 cut(s) 65, 117, 351
BfmI CTRYAG 1 cut(s) 150
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 5 cut(s) 227, 230, 331, 334, 342
BlsI GCNGC 5 cut(s) 228, 231, 332, 335, 343
Bme1390I CCNGG 1 cut(s) 284
Bme18I GGWCC 1 cut(s) 280
BmeRI GACNNNNNGTC 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 2 cut(s) 282, 291
BmrFI CCNGG 1 cut(s) 284
BmrI ACTGGG 1 cut(s) 28
BmsI GCATC 1 cut(s) 419
BmuI ACTGGG 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 445
BpuMI CCSGG 1 cut(s) 284
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bsc4I CCNNNNNNNGG 1 cut(s) 423
Bse1I ACTGG 1 cut(s) 23
Bse3DI GCAATG 2 cut(s) 166, 239
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 423
BseMI GCAATG 2 cut(s) 166, 239
BseNI ACTGG 1 cut(s) 23
BseRI GAGGAG 1 cut(s) 402
BseXI GCAGC 3 cut(s) 328, 342, 345
BsgI GTGCAG 1 cut(s) 296
BshFI GGCC 2 cut(s) 5, 226
BsiHKAI GWGCWC 1 cut(s) 374
BsiSI CCGG 1 cut(s) 284
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 1 cut(s) 423
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 2 cut(s) 5, 226
Bsp1286I GDGCHC 1 cut(s) 374
BspACI CCGC 6 cut(s) 227, 230, 244, 249, 258, 272
BspANI GGCC 2 cut(s) 5, 226
BspLI GGNNCC 2 cut(s) 282, 291
BspMI ACCTGC 1 cut(s) 268
BsrDI GCAATG 2 cut(s) 166, 239
BsrI ACTGG 1 cut(s) 23
BstC8I GCNNGC 2 cut(s) 7, 260
BstF5I GGATG 1 cut(s) 57
BstMWI GCNNNNNNNGC 3 cut(s) 255, 369, 429
BstSCI CCNGG 1 cut(s) 282
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 3 cut(s) 328, 342, 345
BstXI CCANNNNNNTGG 1 cut(s) 317
BsuRI GGCC 2 cut(s) 5, 226
BtsCI GGATG 1 cut(s) 57
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 372
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 2 cut(s) 7, 260
Cfr13I GGNCC 1 cut(s) 280
Csp6I GTAC 1 cut(s) 87
CviQI GTAC 1 cut(s) 87
DriI GACNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 1 cut(s) 3
Eam1105I GACNNNNNGTC 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 280
Eco57I CTGAAG 3 cut(s) 67, 79, 162
EcoO109I RGGNCCY 1 cut(s) 280
FaiI YATR 3 cut(s) 47, 104, 419
FaqI GGGAC 1 cut(s) 266
FauI CCCGC 1 cut(s) 251
Fnu4HI GCNGC 5 cut(s) 227, 230, 331, 334, 342
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 5 cut(s) 227, 230, 331, 334, 342
FspBI CTAG 3 cut(s) 65, 117, 351
GluI GCNGC 5 cut(s) 227, 230, 331, 334, 342
GsuI CTGGAG 1 cut(s) 445
HaeIII GGCC 2 cut(s) 5, 226
HapII CCGG 1 cut(s) 284
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 79
HpaII CCGG 1 cut(s) 284
Hpy166II GTNNAC 1 cut(s) 202
Hpy188III TCNNGA 1 cut(s) 384
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 1 cut(s) 398
HpyCH4V TGCA 2 cut(s) 277, 432
HpyF10VI GCNNNNNNNGC 3 cut(s) 255, 369, 429
LmnI GCTCC 2 cut(s) 295, 320
Lsp1109I GCAGC 3 cut(s) 328, 342, 345
LweI GCATC 1 cut(s) 419
MaeI CTAG 3 cut(s) 65, 117, 351
MboII GAAGA 1 cut(s) 442
MhlI GDGCHC 1 cut(s) 374
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 123, 213, 406
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 152
MnlI CCTC 5 cut(s) 149, 166, 234, 380, 383
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 315
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 284
MspR9I CCNGG 1 cut(s) 284
MwoI GCNNNNNNNGC 3 cut(s) 255, 369, 429
NciI CCSGG 1 cut(s) 284
NlaIV GGNNCC 2 cut(s) 282, 291
PflMI CCANNNNNTGG 1 cut(s) 423
PkrI GCNGC 5 cut(s) 228, 231, 332, 335, 343
PpuMI RGGWCCY 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 280
PspN4I GGNNCC 2 cut(s) 282, 291
PspPI GGNCC 1 cut(s) 280
PspPPI RGGWCCY 1 cut(s) 280
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
RseI CAYNNNNRTG 1 cut(s) 315
SatI GCNGC 5 cut(s) 227, 230, 331, 334, 342
Sau96I GGNCC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 284
SduI GDGCHC 1 cut(s) 374
SetI ASST 8 cut(s) 11, 83, 115, 138, 142, 282, 338, 425
SfaNI GCATC 1 cut(s) 419
SfcI CTRYAG 1 cut(s) 150
SinI GGWCC 1 cut(s) 280
SmiMI CAYNNNNRTG 1 cut(s) 315
SpeI ACTAGT 1 cut(s) 64
Sse9I AATT 3 cut(s) 123, 213, 406
SsiI CCGC 6 cut(s) 227, 230, 244, 249, 258, 272
SspMI CTAG 3 cut(s) 65, 117, 351
StyD4I CCNGG 1 cut(s) 282
TaqI TCGA 1 cut(s) 404
TasI AATT 3 cut(s) 123, 213, 406
TauI GCSGC 2 cut(s) 229, 232
TscAI CASTG 1 cut(s) 379
TseI GCWGC 3 cut(s) 330, 333, 341
TspDTI ATGAA 1 cut(s) 91
TspRI CASTG 1 cut(s) 379
Van91I CCANNNNNTGG 1 cut(s) 423
VpaK11BI GGWCC 1 cut(s) 280
XspI CTAG 3 cut(s) 65, 117, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.