RLG00000010759

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008052
Physical Location & Seq
Reverse (-)
4170431 .. 4173186
2756 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010759

Sequence Viewer

Length: 1239 bp
ATGGCTTCTCTGGAGTCGGAGGCCGGCTCTGTACGGACCGCCGAATCTTCCAATGAAATCAAACAGAGAGCCAGAAAAAATAGGACGAAGAAGAAGAAGAAGAAGACCAAAAACGGTGCGTTTCGTTACCTCCCCAAATTTCGGTGCTTTAGTTTAAAATCGGAAACCACTGACGGGAAGGGGAGTTTCGATATGGAGGTGGAGGATGAAGCGGGTCGGGTTGGAATGAACCCGACCCACCTTGTTATAATGGTTAATGGGCTTATAGGCAGTGCCGAGGATTGGAGGTATGCGGCGACCCAGTTTGTGAAGAGATACCCAGAAGATGTTGTTGTGCATTGCAGTGAATGTAATTGTTCAACGTTGACATTTGATGGTGTGGATGTAATGGGAGAGAGATTAGCAGAAGAGGTTAAATGTGTAATAAAACGCCACCAAAGTGTTCAGAAGATCTCGTTTGTTGGTCACTCATTAGGTGGCCTTATAGCAAGGTATGCCATTGGCAGGCTTTATGAACGCGACACAACAAGGGAACTTTCTCAGGAAAATGGAGAATGTAGGGGCAATGGATTTGAAGATCCATCGCTGGAACAGAAAGCGAAAAGCAAAATTGCTGGGCTGGAGCCGGTGAATTTTATAACATCTGCAACTCCGCACCTCGGTTCATGGGGGCATAAACAGGTTCCAGTGTTTTGTGGCTTTCAAATCCTTGAAAAAGCAGCAGCTAGTACTTCATGGTGTTTAGGTAGAACTGGAAGACATCTGTTTTTAACTGATAGGGATAAAGGAAAACCTCCCCTTTTACTTCAGATGGTTTATGATTCTGAAGATCTTAAATTCTTATCTGCATTGAAGTCCTTCAGGCATCATGTTGCCTATGCAAATGTTCATTATGATCACCTCGTGGGATGGAGTACGTCTTCTATACGTCGTAAGAAGGAGCTGCCAAAGCTTAAACATTTATCTAGAGACAACAAATATCCACATATTGTAAATGAGGACATGATAAACACCGCTTGTACTCAAGAAGAAGTTGCTTTGGACGACAACGTGAATATTGACTTCGAAGAGGAAATGATCAGAGGTTTGACCAAGGTGAGCTGGGAAAGGATTGATGTAAGTTTTAGTGGAAGTAGACAAAAGTACCTTGCACATAATACCATTCAGGTGAAAACTTATTGTATACATTCTGATGGAGCTGATGTGATCCAACACATGGTGGACAATTTTTGTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

413

Amino Acids

46.59

Weight (kDa)

8.51

Isoelectric Point (pI)

37.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 77 - 306 1.5e-64 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 248, 638
AccB7I CCANNNNNTGG 1 cut(s) 1216
AccI GTMKAC 2 cut(s) 1135, 1183
AccII CGCG 1 cut(s) 519
AciI CCGC 5 cut(s) 39, 212, 293, 653, 1014
AclI AACGTT 1 cut(s) 362
AclWI GGATC 2 cut(s) 572, 1201
AcsI RAATTY 3 cut(s) 137, 631, 836
AcuI CTGAAG 3 cut(s) 791, 844, 846
AdeI CACNNNGTG 2 cut(s) 904, 1219
AfaI GTAC 5 cut(s) 33, 730, 916, 1021, 1145
AfiI CCNNNNNNNGG 6 cut(s) 141, 174, 282, 504, 659, 1216
AgsI TTSAA 5 cut(s) 360, 575, 704, 713, 853
AleI CACNNNNGTG 1 cut(s) 438
AluBI AGCT 5 cut(s) 725, 943, 952, 1101, 1199
AluI AGCT 5 cut(s) 725, 943, 952, 1101, 1199
Alw26I GTCTC 1 cut(s) 963
AlwI GGATC 2 cut(s) 572, 1201
AoxI GGCC 2 cut(s) 21, 478
ApeKI GCWGC 3 cut(s) 719, 722, 943
ApoI RAATTY 3 cut(s) 137, 631, 836
Asp700I GAANNNNTTC 1 cut(s) 857
AspS9I GGNCC 1 cut(s) 36
AsuHPI GGTGA 4 cut(s) 640, 890, 1108, 1180
AsuII TTCGAA 1 cut(s) 1065
AvaII GGWCC 1 cut(s) 36
BaeI ACNNNNGTAYC 2 cut(s) 1127, 1160
BauI CACGAG 1 cut(s) 902
BbsI GAAGAC 3 cut(s) 110, 763, 912
BbvI GCAGC 3 cut(s) 731, 734, 930
BccI CCATC 5 cut(s) 368, 589, 805, 903, 1187
BclI TGATCA 2 cut(s) 895, 1077
BcoDI GTCTC 1 cut(s) 963
BfaI CTAG 2 cut(s) 726, 966
BglII AGATCT 2 cut(s) 450, 829
BisI GCNGC 4 cut(s) 294, 720, 723, 944
BlsI GCNGC 4 cut(s) 295, 721, 724, 945
BmcAI AGTACT 1 cut(s) 730
Bme18I GGWCC 1 cut(s) 36
BmgT120I GGNCC 1 cut(s) 36
BmiI GGNNCC 2 cut(s) 624, 684
BmrI ACTGGG 1 cut(s) 295
BmsI GCATC 1 cut(s) 874
BmuI ACTGGG 1 cut(s) 295
BpiI GAAGAC 3 cut(s) 110, 763, 912
BplI GAGNNNNNCTC 2 cut(s) 11, 43
BpmI CTGGAG 2 cut(s) 32, 641
Bpu14I TTCGAA 1 cut(s) 1065
BpuEI CTTGAG 1 cut(s) 1008
BsaJI CCNNGG 3 cut(s) 276, 658, 1092
BsaXI ACNNNNNCTCC 2 cut(s) 1188, 1218
Bsc4I CCNNNNNNNGG 6 cut(s) 141, 174, 282, 504, 659, 1216
Bse118I RCCGGY 2 cut(s) 23, 625
Bse1I ACTGG 3 cut(s) 301, 686, 757
Bse3DI GCAATG 2 cut(s) 337, 571
BseDI CCNNGG 3 cut(s) 276, 658, 1092
BseGI GGATG 3 cut(s) 211, 388, 914
BseLI CCNNNNNNNGG 6 cut(s) 141, 174, 282, 504, 659, 1216
BseMI GCAATG 2 cut(s) 337, 571
BseMII CTCAG 1 cut(s) 554
BseNI ACTGG 3 cut(s) 301, 686, 757
BseXI GCAGC 3 cut(s) 731, 734, 930
BseYI CCCAGC 2 cut(s) 614, 1101
Bsh1236I CGCG 1 cut(s) 519
BshFI GGCC 2 cut(s) 23, 480
BsiSI CCGG 2 cut(s) 24, 626
BslI CCNNNNNNNGG 6 cut(s) 141, 174, 282, 504, 659, 1216
BsmAI GTCTC 1 cut(s) 963
BsnI GGCC 2 cut(s) 23, 480
Bsp119I TTCGAA 1 cut(s) 1065
Bsp143I GATC 6 cut(s) 450, 577, 829, 895, 1077, 1206
BspACI CCGC 5 cut(s) 39, 212, 293, 653, 1014
BspANI GGCC 2 cut(s) 23, 480
BspCNI CTCAG 1 cut(s) 553
BspFNI CGCG 1 cut(s) 519
BspLI GGNNCC 2 cut(s) 624, 684
BspPI GGATC 2 cut(s) 572, 1201
BspT104I TTCGAA 1 cut(s) 1065
BsrDI GCAATG 2 cut(s) 337, 571
BsrFI RCCGGY 2 cut(s) 23, 625
BsrI ACTGG 3 cut(s) 301, 686, 757
BssAI RCCGGY 2 cut(s) 23, 625
BssECI CCNNGG 3 cut(s) 276, 658, 1092
BssMI GATC 6 cut(s) 450, 577, 829, 895, 1077, 1206
BssNAI GTATAC 1 cut(s) 1184
BssSI CACGAG 1 cut(s) 902
BssT1I CCWWGG 1 cut(s) 1092
Bst1107I GTATAC 1 cut(s) 1184
Bst2BI CACGAG 1 cut(s) 902
Bst4CI ACNGT 1 cut(s) 116
Bst6I CTCTTC 3 cut(s) 305, 402, 1062
BstAPI GCANNNNNTGC 1 cut(s) 494
BstBI TTCGAA 1 cut(s) 1065
BstC8I GCNNGC 2 cut(s) 25, 506
BstDEI CTNAG 1 cut(s) 540
BstF5I GGATG 3 cut(s) 211, 388, 914
BstFNI CGCG 1 cut(s) 519
BstKTI GATC 6 cut(s) 453, 580, 832, 898, 1080, 1209
BstMAI GTCTC 1 cut(s) 963
BstMBI GATC 6 cut(s) 450, 577, 829, 895, 1077, 1206
BstMWI GCNNNNNNNGC 2 cut(s) 494, 949
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 3 cut(s) 731, 734, 930
BstV2I GAAGAC 3 cut(s) 110, 763, 912
BstX2I RGATCY 3 cut(s) 450, 577, 829
BstYI RGATCY 3 cut(s) 450, 577, 829
BstZ17I GTATAC 1 cut(s) 1184
BsuRI GGCC 2 cut(s) 23, 480
BtgZI GCGATG 1 cut(s) 567
BtsCI GGATG 3 cut(s) 211, 388, 914
BtsI GCAGTG 2 cut(s) 277, 349
BtsIMutI CAGTG 4 cut(s) 168, 277, 349, 693
Cac8I GCNNGC 2 cut(s) 25, 506
Cfr10I RCCGGY 2 cut(s) 23, 625
Cfr13I GGNCC 1 cut(s) 36
CpoI CGGWCCG 1 cut(s) 36
Csp6I GTAC 5 cut(s) 32, 729, 915, 1020, 1144
CspI CGGWCCG 1 cut(s) 36
CviAII CATG 5 cut(s) 666, 735, 869, 1003, 1216
CviQI GTAC 5 cut(s) 32, 729, 915, 1020, 1144
DdeI CTNAG 1 cut(s) 540
DpnI GATC 6 cut(s) 452, 579, 831, 897, 1079, 1208
DpnII GATC 6 cut(s) 450, 577, 829, 895, 1077, 1206
DraI TTTAAA 1 cut(s) 156
DraIII CACNNNGTG 2 cut(s) 904, 1219
Eam1104I CTCTTC 3 cut(s) 305, 402, 1062
EarI CTCTTC 3 cut(s) 305, 402, 1062
Eco130I CCWWGG 1 cut(s) 1092
Eco47I GGWCC 1 cut(s) 36
Eco57I CTGAAG 3 cut(s) 791, 844, 846
EcoT14I CCWWGG 1 cut(s) 1092
ErhI CCWWGG 1 cut(s) 1092
FaeI CATG 5 cut(s) 669, 738, 872, 1006, 1219
FatI CATG 5 cut(s) 665, 734, 868, 1002, 1215
FauI CCCGC 1 cut(s) 205
FbaI TGATCA 2 cut(s) 895, 1077
FblI GTMKAC 2 cut(s) 1135, 1183
Fnu4HI GCNGC 4 cut(s) 294, 720, 723, 944
FokI GGATG 3 cut(s) 218, 395, 921
Fsp4HI GCNGC 4 cut(s) 294, 720, 723, 944
FspBI CTAG 2 cut(s) 726, 966
GluI GCNGC 4 cut(s) 294, 720, 723, 944
GsaI CCCAGC 2 cut(s) 618, 1105
GsuI CTGGAG 2 cut(s) 32, 641
HaeIII GGCC 2 cut(s) 23, 480
HapII CCGG 2 cut(s) 24, 626
Hin1II CATG 5 cut(s) 669, 738, 872, 1006, 1219
HincII GTYRAC 1 cut(s) 366
HindII GTYRAC 1 cut(s) 366
HindIII AAGCTT 1 cut(s) 950
HinfI GANTC 3 cut(s) 14, 44, 821
HpaII CCGG 2 cut(s) 24, 626
HphI GGTGA 4 cut(s) 640, 890, 1108, 1180
Hpy166II GTNNAC 4 cut(s) 366, 1136, 1184, 1222
Hpy188I TCNGA 7 cut(s) 19, 163, 447, 810, 826, 1082, 1192
Hpy188III TCNNGA 4 cut(s) 11, 542, 966, 1025
Hpy8I GTNNAC 4 cut(s) 366, 1136, 1184, 1222
Hpy99I CGWCG 1 cut(s) 933
HpyAV CCTTC 3 cut(s) 172, 868, 931
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 4 cut(s) 362, 917, 928, 1050
HpyCH4V TGCA 6 cut(s) 337, 342, 647, 848, 881, 1151
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 949
HpyF3I CTNAG 1 cut(s) 540
HpySE526I ACGT 4 cut(s) 362, 917, 928, 1050
Hsp92II CATG 5 cut(s) 669, 738, 872, 1006, 1219
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Ksp22I TGATCA 2 cut(s) 895, 1077
Kzo9I GATC 6 cut(s) 450, 577, 829, 895, 1077, 1206
LmnI GCTCC 3 cut(s) 622, 940, 1196
Lsp1109I GCAGC 3 cut(s) 731, 734, 930
LweI GCATC 1 cut(s) 874
MaeI CTAG 2 cut(s) 726, 966
MaeII ACGT 4 cut(s) 362, 917, 928, 1050
MaeIII GTNAC 2 cut(s) 125, 464
MalI GATC 6 cut(s) 452, 579, 831, 897, 1079, 1208
MboI GATC 6 cut(s) 450, 577, 829, 895, 1077, 1206
MflI RGATCY 3 cut(s) 450, 577, 829
MluCI AATT 6 cut(s) 137, 352, 609, 631, 836, 1225
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 2 cut(s) 202, 1234
MroNI GCCGGC 1 cut(s) 23
MroXI GAANNNNTTC 1 cut(s) 857
MseI TTAA 6 cut(s) 155, 255, 414, 770, 834, 954
MslI CAYNNNNRTG 4 cut(s) 342, 438, 1166, 1191
MspI CCGG 2 cut(s) 24, 626
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 2 cut(s) 494, 949
NaeI GCCGGC 1 cut(s) 25
NdeII GATC 6 cut(s) 450, 577, 829, 895, 1077, 1206
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 5 cut(s) 669, 738, 872, 1006, 1219
NlaIV GGNNCC 2 cut(s) 624, 684
NmeAIII GCCGAG 1 cut(s) 301
NmuCI GTSAC 1 cut(s) 464
NspV TTCGAA 1 cut(s) 1065
OliI CACNNNNGTG 1 cut(s) 438
PdiI GCCGGC 1 cut(s) 25
PdmI GAANNNNTTC 1 cut(s) 857
PfeI GAWTC 2 cut(s) 44, 821
PflMI CCANNNNNTGG 1 cut(s) 1216
PkrI GCNGC 4 cut(s) 295, 721, 724, 945
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
PsiI TTATAA 2 cut(s) 248, 638
Psp1406I AACGTT 1 cut(s) 362
PspFI CCCAGC 2 cut(s) 614, 1101
PspN4I GGNNCC 2 cut(s) 624, 684
PspPI GGNCC 1 cut(s) 36
PsuI RGATCY 3 cut(s) 450, 577, 829
RsaI GTAC 5 cut(s) 33, 730, 916, 1021, 1145
RsaNI GTAC 5 cut(s) 32, 729, 915, 1020, 1144
RseI CAYNNNNRTG 4 cut(s) 342, 438, 1166, 1191
Rsr2I CGGWCCG 1 cut(s) 36
RsrII CGGWCCG 1 cut(s) 36
SaqAI TTAA 6 cut(s) 155, 255, 414, 770, 834, 954
SatI GCNGC 4 cut(s) 294, 720, 723, 944
Sau3AI GATC 6 cut(s) 450, 577, 829, 895, 1077, 1206
Sau96I GGNCC 1 cut(s) 36
ScaI AGTACT 1 cut(s) 730
SchI GAGTC 1 cut(s) 23
SfaNI GCATC 1 cut(s) 874
SfuI TTCGAA 1 cut(s) 1065
SinI GGWCC 1 cut(s) 36
SmiMI CAYNNNNRTG 4 cut(s) 342, 438, 1166, 1191
SmlI CTYRAG 1 cut(s) 1023
SmoI CTYRAG 1 cut(s) 1023
Sse9I AATT 6 cut(s) 137, 352, 609, 631, 836, 1225
SsiI CCGC 5 cut(s) 39, 212, 293, 653, 1014
SspI AATATT 1 cut(s) 1057
SspMI CTAG 2 cut(s) 726, 966
StyI CCWWGG 1 cut(s) 1092
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 4 cut(s) 365, 920, 931, 1053
TaqI TCGA 2 cut(s) 189, 1065
TasI AATT 6 cut(s) 137, 352, 609, 631, 836, 1225
TatI WGTACW 2 cut(s) 728, 1019
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 2 cut(s) 44, 821
Tru1I TTAA 6 cut(s) 155, 255, 414, 770, 834, 954
Tru9I TTAA 6 cut(s) 155, 255, 414, 770, 834, 954
TscAI CASTG 4 cut(s) 175, 277, 349, 693
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 3 cut(s) 719, 722, 943
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 7 cut(s) 69, 222, 242, 528, 654, 723, 878
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 4 cut(s) 175, 277, 349, 693
Van91I CCANNNNNTGG 1 cut(s) 1216
VpaK11BI GGWCC 1 cut(s) 36
XapI RAATTY 3 cut(s) 137, 631, 836
XbaI TCTAGA 1 cut(s) 965
XmiI GTMKAC 2 cut(s) 1135, 1183
XmnI GAANNNNTTC 1 cut(s) 857
XspI CTAG 2 cut(s) 726, 966
ZrmI AGTACT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.