Rh6BG435300

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0008052
Physical Location & Seq
Reverse (-)
65538433 .. 65541521
3089 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG435300.1

Sequence Viewer

Length: 1242 bp
ATGGCTTCTCTGGAGTCGGAGGCCGGCTCTGTACGGACCGCCGAATCTTCCAATGAAATCAAACAGAGAGCCAGAAAAAATAGGACCAACAAGAAGAAGACCAAAAACGGTGCGTTTCGTTACCTCCCCAAATTTCGGTGCTTTAGTTTAAAATCGGAAACTACTGACGGTCACGGGAAGGGGAAGGGGAGTTTCGATATGGAGGTGGAGGATGAAGCGGGTCGGGGAATCAACCCGACCCACCTTGTCATAATGGTTAATGGGCTTATAGGCAGTGCCGAGGATTGGAGGTATGCAGCGACCCAGTTTGTGAAGAGATACCCAGAAGATGTTGTTGTGCATTGCAGTGAATGTAATTGTTCAACGTTGACATTTGATGGTGTGGATGTAATGGGGGAGAGATTAGCAGAAGAGGTTAAATCTGTAATAAAACGCCACCAAAGTGTTCAGAAGATCTCGTTTGTTGGTCACTCATTAGGTGGCCTTATAGCAAGGTATGCCATTGGCAGGCTTTATGAACGCGACACAACAAGGGAACTTTCTCAGGAAAATGGAGAATGTAGGGGCAATGGATTTGAGGATCCATCGCTGGAACAGAAAGCGAAAAGCAAAATTGCTGGGCTGGAGCCGGTGAATTTTATAACGTCTGCAACTCCGCACCTCGGTTCATGGGGGCATAAACAGGTTCCAGTGTTTTGTGGCTTTCAAATCCTTGAAAAAGCAGCAGCTCGTACTTCATGGTGTTTAGGTAGAACTGGAAGACATCTGTTTTTAACTGATAGGGATAAAGGAAAACCTCCCCTTTTACTTCAGATGGTTTATGATTCTGAAGATCTTAAATTCTTATCTGCATTGAAGTCCTTCAGGCGTCATGTTGCCTATGCAAATGCTCATTATGATCACCTCGTGGGATGGAGTACGTCTTCTATACGTCCTAAGAAGGAGCTGCCAAAGCTTAAACATTTATCCAGAGACAACAAATATCCACATATTGTAAATGAGGACATGATAAACACCGCTAGTACTCAAGAAGAAGTTGCTTTGGAGGACGACGTGAATATTGACTTCGAAGAGGAAATGATCAGAGGTTTGACCAAGGTGAGCTGGGAAAGGATTGATGTAAGTTTTAGTGGAAGTAGACAAAAGTACCTTGCACATAATACCATTCAGGTGAAAACTTATTGTATACATTCTGATGGAGCTGATGTGATCCAACACATGGTGGACAATTTTCGTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

413

Amino Acids

46.62

Weight (kDa)

8.48

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 78 - 307 3.4e-65 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 641
AccB7I CCANNNNNTGG 1 cut(s) 1219
AccI GTMKAC 2 cut(s) 1138, 1186
AccII CGCG 1 cut(s) 522
AciI CCGC 4 cut(s) 39, 218, 656, 1017
AclI AACGTT 1 cut(s) 365
AclWI GGATC 3 cut(s) 575, 588, 1204
AcsI RAATTY 3 cut(s) 131, 634, 839
AcuI CTGAAG 3 cut(s) 794, 847, 849
AcyI GRCGYC 1 cut(s) 868
AdeI CACNNNGTG 2 cut(s) 907, 1222
AfaI GTAC 5 cut(s) 33, 733, 919, 1024, 1148
AfiI CCNNNNNNNGG 5 cut(s) 135, 285, 507, 662, 1219
AgsI TTSAA 4 cut(s) 363, 707, 716, 856
AjiI CACGTC 1 cut(s) 1054
AleI CACNNNNGTG 1 cut(s) 441
AluBI AGCT 5 cut(s) 728, 946, 955, 1104, 1202
AluI AGCT 5 cut(s) 728, 946, 955, 1104, 1202
Alw26I GTCTC 1 cut(s) 966
AlwI GGATC 3 cut(s) 575, 588, 1204
AoxI GGCC 2 cut(s) 21, 481
ApeKI GCWGC 4 cut(s) 296, 722, 725, 946
ApoI RAATTY 3 cut(s) 131, 634, 839
Asp700I GAANNNNTTC 1 cut(s) 860
AspS9I GGNCC 2 cut(s) 36, 84
AsuHPI GGTGA 4 cut(s) 643, 893, 1111, 1183
AsuII TTCGAA 1 cut(s) 1068
AvaII GGWCC 2 cut(s) 36, 84
BaeI ACNNNNGTAYC 2 cut(s) 1130, 1163
BamHI GGATCC 1 cut(s) 580
BauI CACGAG 1 cut(s) 905
BbsI GAAGAC 3 cut(s) 104, 766, 915
BbvI GCAGC 4 cut(s) 308, 734, 737, 933
BccI CCATC 5 cut(s) 371, 592, 808, 906, 1190
BclI TGATCA 2 cut(s) 898, 1080
BcoDI GTCTC 1 cut(s) 966
BfaI CTAG 1 cut(s) 1020
BglII AGATCT 2 cut(s) 453, 832
BisI GCNGC 4 cut(s) 297, 723, 726, 947
BlsI GCNGC 4 cut(s) 298, 724, 727, 948
BmcAI AGTACT 1 cut(s) 1024
Bme18I GGWCC 2 cut(s) 36, 84
BmgBI CACGTC 1 cut(s) 1054
BmgT120I GGNCC 2 cut(s) 36, 84
BmiI GGNNCC 3 cut(s) 582, 627, 687
BmrI ACTGGG 1 cut(s) 298
BmuI ACTGGG 1 cut(s) 298
BpiI GAAGAC 3 cut(s) 104, 766, 915
BplI GAGNNNNNCTC 2 cut(s) 11, 43
BpmI CTGGAG 2 cut(s) 32, 644
Bpu14I TTCGAA 1 cut(s) 1068
BpuEI CTTGAG 1 cut(s) 1011
BsaHI GRCGYC 1 cut(s) 868
BsaJI CCNNGG 3 cut(s) 279, 661, 1095
BsaXI ACNNNNNCTCC 2 cut(s) 1191, 1221
Bsc4I CCNNNNNNNGG 5 cut(s) 135, 285, 507, 662, 1219
Bse118I RCCGGY 2 cut(s) 23, 628
Bse1I ACTGG 3 cut(s) 304, 689, 760
Bse3DI GCAATG 2 cut(s) 340, 574
BseDI CCNNGG 3 cut(s) 279, 661, 1095
BseGI GGATG 3 cut(s) 217, 391, 917
BseLI CCNNNNNNNGG 5 cut(s) 135, 285, 507, 662, 1219
BseMI GCAATG 2 cut(s) 340, 574
BseMII CTCAG 1 cut(s) 557
BseNI ACTGG 3 cut(s) 304, 689, 760
BseXI GCAGC 4 cut(s) 308, 734, 737, 933
BseYI CCCAGC 2 cut(s) 617, 1104
Bsh1236I CGCG 1 cut(s) 522
BshFI GGCC 2 cut(s) 23, 483
BsiSI CCGG 2 cut(s) 24, 629
BslI CCNNNNNNNGG 5 cut(s) 135, 285, 507, 662, 1219
BsmAI GTCTC 1 cut(s) 966
BsnI GGCC 2 cut(s) 23, 483
Bsp119I TTCGAA 1 cut(s) 1068
Bsp143I GATC 6 cut(s) 453, 580, 832, 898, 1080, 1209
BspACI CCGC 4 cut(s) 39, 218, 656, 1017
BspANI GGCC 2 cut(s) 23, 483
BspCNI CTCAG 1 cut(s) 556
BspFNI CGCG 1 cut(s) 522
BspLI GGNNCC 3 cut(s) 582, 627, 687
BspPI GGATC 3 cut(s) 575, 588, 1204
BspT104I TTCGAA 1 cut(s) 1068
BsrDI GCAATG 2 cut(s) 340, 574
BsrFI RCCGGY 2 cut(s) 23, 628
BsrI ACTGG 3 cut(s) 304, 689, 760
BssAI RCCGGY 2 cut(s) 23, 628
BssECI CCNNGG 3 cut(s) 279, 661, 1095
BssMI GATC 6 cut(s) 453, 580, 832, 898, 1080, 1209
BssNAI GTATAC 1 cut(s) 1187
BssNI GRCGYC 1 cut(s) 868
BssSI CACGAG 1 cut(s) 905
BssT1I CCWWGG 1 cut(s) 1095
Bst1107I GTATAC 1 cut(s) 1187
Bst2BI CACGAG 1 cut(s) 905
Bst4CI ACNGT 2 cut(s) 110, 170
Bst6I CTCTTC 3 cut(s) 308, 405, 1065
BstACI GRCGYC 1 cut(s) 868
BstAPI GCANNNNNTGC 1 cut(s) 497
BstBI TTCGAA 1 cut(s) 1068
BstC8I GCNNGC 2 cut(s) 25, 509
BstDEI CTNAG 2 cut(s) 543, 936
BstF5I GGATG 3 cut(s) 217, 391, 917
BstFNI CGCG 1 cut(s) 522
BstKTI GATC 6 cut(s) 456, 583, 835, 901, 1083, 1212
BstMAI GTCTC 1 cut(s) 966
BstMBI GATC 6 cut(s) 453, 580, 832, 898, 1080, 1209
BstMWI GCNNNNNNNGC 2 cut(s) 497, 952
BstUI CGCG 1 cut(s) 522
BstV1I GCAGC 4 cut(s) 308, 734, 737, 933
BstV2I GAAGAC 3 cut(s) 104, 766, 915
BstX2I RGATCY 3 cut(s) 453, 580, 832
BstYI RGATCY 3 cut(s) 453, 580, 832
BstZ17I GTATAC 1 cut(s) 1187
BsuRI GGCC 2 cut(s) 23, 483
BtgZI GCGATG 1 cut(s) 570
BtrI CACGTC 1 cut(s) 1054
BtsCI GGATG 3 cut(s) 217, 391, 917
BtsI GCAGTG 2 cut(s) 280, 352
BtsIMutI CAGTG 3 cut(s) 280, 352, 696
Cac8I GCNNGC 2 cut(s) 25, 509
Cfr10I RCCGGY 2 cut(s) 23, 628
Cfr13I GGNCC 2 cut(s) 36, 84
CpoI CGGWCCG 1 cut(s) 36
CseI GACGC 1 cut(s) 857
Csp6I GTAC 5 cut(s) 32, 732, 918, 1023, 1147
CspI CGGWCCG 1 cut(s) 36
CviAII CATG 5 cut(s) 669, 738, 872, 1006, 1219
CviQI GTAC 5 cut(s) 32, 732, 918, 1023, 1147
DdeI CTNAG 2 cut(s) 543, 936
DpnI GATC 6 cut(s) 455, 582, 834, 900, 1082, 1211
DpnII GATC 6 cut(s) 453, 580, 832, 898, 1080, 1209
DraI TTTAAA 1 cut(s) 150
DraIII CACNNNGTG 2 cut(s) 907, 1222
Eam1104I CTCTTC 3 cut(s) 308, 405, 1065
EarI CTCTTC 3 cut(s) 308, 405, 1065
Eco130I CCWWGG 1 cut(s) 1095
Eco47I GGWCC 2 cut(s) 36, 84
Eco57I CTGAAG 3 cut(s) 794, 847, 849
EcoT14I CCWWGG 1 cut(s) 1095
ErhI CCWWGG 1 cut(s) 1095
FaeI CATG 5 cut(s) 672, 741, 875, 1009, 1222
FatI CATG 5 cut(s) 668, 737, 871, 1005, 1218
FauI CCCGC 1 cut(s) 211
FbaI TGATCA 2 cut(s) 898, 1080
FblI GTMKAC 2 cut(s) 1138, 1186
Fnu4HI GCNGC 4 cut(s) 297, 723, 726, 947
FokI GGATG 3 cut(s) 224, 398, 924
Fsp4HI GCNGC 4 cut(s) 297, 723, 726, 947
FspBI CTAG 1 cut(s) 1020
GluI GCNGC 4 cut(s) 297, 723, 726, 947
GsaI CCCAGC 2 cut(s) 621, 1108
GsuI CTGGAG 2 cut(s) 32, 644
HaeIII GGCC 2 cut(s) 23, 483
HapII CCGG 2 cut(s) 24, 629
HgaI GACGC 1 cut(s) 857
Hin1I GRCGYC 1 cut(s) 868
Hin1II CATG 5 cut(s) 672, 741, 875, 1009, 1222
HincII GTYRAC 1 cut(s) 369
HindII GTYRAC 1 cut(s) 369
HindIII AAGCTT 1 cut(s) 953
HinfI GANTC 4 cut(s) 14, 44, 228, 824
HpaII CCGG 2 cut(s) 24, 629
HphI GGTGA 4 cut(s) 643, 893, 1111, 1183
Hpy166II GTNNAC 4 cut(s) 369, 1139, 1187, 1225
Hpy188I TCNGA 7 cut(s) 19, 157, 450, 813, 829, 1085, 1195
Hpy188III TCNNGA 4 cut(s) 11, 545, 969, 1028
Hpy8I GTNNAC 4 cut(s) 369, 1139, 1187, 1225
Hpy99I CGWCG 1 cut(s) 1055
HpyAV CCTTC 4 cut(s) 172, 178, 871, 934
HpyCH4III ACNGT 2 cut(s) 110, 170
HpyCH4IV ACGT 5 cut(s) 365, 644, 920, 931, 1053
HpyCH4V TGCA 7 cut(s) 296, 340, 345, 650, 851, 884, 1154
HpyF10VI GCNNNNNNNGC 2 cut(s) 497, 952
HpyF3I CTNAG 2 cut(s) 543, 936
HpySE526I ACGT 5 cut(s) 365, 644, 920, 931, 1053
Hsp92I GRCGYC 1 cut(s) 868
Hsp92II CATG 5 cut(s) 672, 741, 875, 1009, 1222
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Ksp22I TGATCA 2 cut(s) 898, 1080
Kzo9I GATC 6 cut(s) 453, 580, 832, 898, 1080, 1209
LmnI GCTCC 3 cut(s) 625, 943, 1199
Lsp1109I GCAGC 4 cut(s) 308, 734, 737, 933
MaeI CTAG 1 cut(s) 1020
MaeII ACGT 5 cut(s) 365, 644, 920, 931, 1053
MaeIII GTNAC 3 cut(s) 119, 170, 467
MalI GATC 6 cut(s) 455, 582, 834, 900, 1082, 1211
MboI GATC 6 cut(s) 453, 580, 832, 898, 1080, 1209
MflI RGATCY 3 cut(s) 453, 580, 832
MluCI AATT 6 cut(s) 131, 355, 612, 634, 839, 1228
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 1237
MroNI GCCGGC 1 cut(s) 23
MroXI GAANNNNTTC 1 cut(s) 860
MseI TTAA 6 cut(s) 149, 258, 417, 773, 837, 957
MslI CAYNNNNRTG 4 cut(s) 345, 441, 1169, 1194
MspI CCGG 2 cut(s) 24, 629
MvnI CGCG 1 cut(s) 522
MwoI GCNNNNNNNGC 2 cut(s) 497, 952
NaeI GCCGGC 1 cut(s) 25
NdeII GATC 6 cut(s) 453, 580, 832, 898, 1080, 1209
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 5 cut(s) 672, 741, 875, 1009, 1222
NlaIV GGNNCC 3 cut(s) 582, 627, 687
NmeAIII GCCGAG 1 cut(s) 304
NmuCI GTSAC 2 cut(s) 170, 467
NspV TTCGAA 1 cut(s) 1068
OliI CACNNNNGTG 1 cut(s) 441
PdiI GCCGGC 1 cut(s) 25
PdmI GAANNNNTTC 1 cut(s) 860
PfeI GAWTC 3 cut(s) 44, 228, 824
PflMI CCANNNNNTGG 1 cut(s) 1219
PkrI GCNGC 4 cut(s) 298, 724, 727, 948
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
PsiI TTATAA 1 cut(s) 641
Psp1406I AACGTT 1 cut(s) 365
PspFI CCCAGC 2 cut(s) 617, 1104
PspN4I GGNNCC 3 cut(s) 582, 627, 687
PspPI GGNCC 2 cut(s) 36, 84
PsuI RGATCY 3 cut(s) 453, 580, 832
RsaI GTAC 5 cut(s) 33, 733, 919, 1024, 1148
RsaNI GTAC 5 cut(s) 32, 732, 918, 1023, 1147
RseI CAYNNNNRTG 4 cut(s) 345, 441, 1169, 1194
Rsr2I CGGWCCG 1 cut(s) 36
RsrII CGGWCCG 1 cut(s) 36
SaqAI TTAA 6 cut(s) 149, 258, 417, 773, 837, 957
SatI GCNGC 4 cut(s) 297, 723, 726, 947
Sau3AI GATC 6 cut(s) 453, 580, 832, 898, 1080, 1209
Sau96I GGNCC 2 cut(s) 36, 84
ScaI AGTACT 1 cut(s) 1024
SchI GAGTC 1 cut(s) 23
SfuI TTCGAA 1 cut(s) 1068
SinI GGWCC 2 cut(s) 36, 84
SmiMI CAYNNNNRTG 4 cut(s) 345, 441, 1169, 1194
SmlI CTYRAG 1 cut(s) 1026
SmoI CTYRAG 1 cut(s) 1026
Sse9I AATT 6 cut(s) 131, 355, 612, 634, 839, 1228
SsiI CCGC 4 cut(s) 39, 218, 656, 1017
SspI AATATT 1 cut(s) 1060
SspMI CTAG 1 cut(s) 1020
StyI CCWWGG 1 cut(s) 1095
TaaI ACNGT 2 cut(s) 110, 170
TaiI ACGT 5 cut(s) 368, 647, 923, 934, 1056
TaqI TCGA 2 cut(s) 195, 1068
TasI AATT 6 cut(s) 131, 355, 612, 634, 839, 1228
TatI WGTACW 1 cut(s) 1022
TfiI GAWTC 3 cut(s) 44, 228, 824
Tru1I TTAA 6 cut(s) 149, 258, 417, 773, 837, 957
Tru9I TTAA 6 cut(s) 149, 258, 417, 773, 837, 957
TscAI CASTG 3 cut(s) 280, 352, 696
TseFI GTSAC 2 cut(s) 170, 467
TseI GCWGC 4 cut(s) 296, 722, 725, 946
Tsp45I GTSAC 2 cut(s) 170, 467
TspDTI ATGAA 5 cut(s) 69, 228, 531, 657, 726
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 3 cut(s) 280, 352, 696
Van91I CCANNNNNTGG 1 cut(s) 1219
VpaK11BI GGWCC 2 cut(s) 36, 84
XapI RAATTY 3 cut(s) 131, 634, 839
XmiI GTMKAC 2 cut(s) 1138, 1186
XmnI GAANNNNTTC 1 cut(s) 860
XspI CTAG 1 cut(s) 1020
ZrmI AGTACT 1 cut(s) 1024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.