MD15G1089000.v1.1

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008052
Physical Location & Seq
Reverse (-)
6196524 .. 6200574
4051 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1089000.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGGCGGCAATGGAGGTGGAGATCGGGGGCGATGGGGTCGTACTGAAAGAGGAGCCGTCAAGCGTGATCAAACACAGAGGCGGCAAGAAAAACGCGAGGAAAAAGAAAAGGAGCAAAAGCAAGGGGACGATGACGACAAGCGGGAAAAAAGAAACGTTTTTTCACATGCCGAAGCTTCGGTGCTTCAGCTCGAAACCCGAAACGGATACGAAGGGGGGTTTCAAGGGGGGTTTCGATATGGAGGTGGAGGATGAAACGGGTGGAAAAAGGAGCCCGACCCATTTGATTATTTTGGTGAATGGTCTCATAGGCAGTGCTGAGAATTGGAAATATGCAGCAAAGCAGTTTCTGAAGAGATACCCAGAAGATGTTATTGCTCACTGTAGTGAATGCAATCACTCAATGTTGACCTTTGATGGTGTTGATGTAATGGGAGAGAGATTAGCAGAAGAGGTTATTTCTGTGATAAAACGTCACCCTAGTGTTCAGAAGATCTCGTTTGTTGGTCATTCGTTAGGCGGGCTTATTGCAAGGTATGCCATTGGAAGGCTTTATGAACGAGACATCACTGAAGAACTGTCTCAAGAAAATGGAGAATATAGGAGAAATGGAGTTGAGAATCCGTTGCTGGAACACAAAGTCAAAGGCAAAATTGCTGGACTGGAGCCTGTGAATTTTATAACGTCTGCCACTCCGCACCTTGGTTCCAGAGGGCATAATCAGGTTCCACTGTTTTGTGGCGTAAAACCCCTTGAAAAAGTAGCAGCTCGTGCTTCATGGTGTCTAGGTAGAAGTGGAAAACATCTGTTTTTAACTGATAAGAAGGATGGAAAACCTCCCCTTCTTCTTCAGATGGTCAATGATTCTGAAGATCTTAAATTCATATCTGCACTGCAGTCTTTCAAGCGCTGTGTTACCTATGCAAATGTTCGTTTTGACTCCCTTGTCGGATGGAGTACATCATCTCTACGGCGTAAGAACGAGCTGCCAAAGCTTAAACATTTATCTAGAGAAGAAAACTATCCACATATTATAAATGTGGAGACAATTCAAAGTTCAAGTTCCCGAGAAGAATTACCGTTGGAAGCCATACCCAGTGGGCGCAAGAAAATTGACTTCGAAGAGGAAATGATCAGAAACTTGACCAAAATAAGCTGGGAACGGGTGGATGTGAACTTCAGAGGAAGTAAACAAAGATACCTTGCGCATGGTACCATTCAGGTGCAAAACGATTGTCTATACTCTGATGGGGCTGATGTGATCCAACACATGGTCGACAATTTTCTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

431

Amino Acids

48.3

Weight (kDa)

9.15

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 91 - 320 9.4e-65 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 680, 1034
AasI GACNNNNNNGTC 1 cut(s) 944
Acc16I TGCGCA 1 cut(s) 1206
Acc65I GGTACC 1 cut(s) 1211
AccB1I GGYRCC 1 cut(s) 1211
AccB7I CCANNNNNTGG 1 cut(s) 1270
AccI GTMKAC 1 cut(s) 1275
AccII CGCG 1 cut(s) 95
AciI CCGC 5 cut(s) 5, 81, 141, 519, 695
AclI AACGTT 1 cut(s) 155
AclWI GGATC 1 cut(s) 1255
AcsI RAATTY 2 cut(s) 673, 878
AcuI CTGAAG 6 cut(s) 169, 371, 591, 833, 888, 1162
AfaI GTAC 3 cut(s) 42, 958, 1213
AfeI AGCGCT 1 cut(s) 908
AfiI CCNNNNNNNGG 3 cut(s) 546, 701, 1270
AgsI TTSAA 5 cut(s) 223, 755, 904, 1052, 1059
AleI CACNNNNGTG 2 cut(s) 384, 480
AluBI AGCT 6 cut(s) 175, 189, 767, 985, 994, 1155
AluI AGCT 6 cut(s) 175, 189, 767, 985, 994, 1155
Alw26I GTCTC 4 cut(s) 308, 555, 585, 1037
AlwI GGATC 1 cut(s) 1255
AlwNI CAGNNNCTG 1 cut(s) 349
Ama87I CYCGRG 1 cut(s) 1065
Aor51HI AGCGCT 1 cut(s) 908
ApeKI GCWGC 3 cut(s) 335, 764, 985
ApoI RAATTY 2 cut(s) 673, 878
ArsI GACNNNNNNTTYG 2 cut(s) 1219, 1251
Asp718I GGTACC 1 cut(s) 1211
AspLEI GCGC 3 cut(s) 909, 1104, 1207
AsuHPI GGTGA 2 cut(s) 307, 467
AsuII TTCGAA 1 cut(s) 1119
AvaI CYCGRG 1 cut(s) 1065
BanI GGYRCC 1 cut(s) 1211
BanII GRGCYC 1 cut(s) 275
BauI CACGAG 1 cut(s) 768
BbvI GCAGC 3 cut(s) 347, 776, 972
BccI CCATC 6 cut(s) 26, 410, 821, 847, 945, 1241
BceAI ACGGC 2 cut(s) 40, 986
BciVI GTATCC 1 cut(s) 199
BclI TGATCA 2 cut(s) 66, 1131
BcoDI GTCTC 4 cut(s) 308, 555, 585, 1037
BfaI CTAG 3 cut(s) 480, 785, 1008
BfmI CTRYAG 2 cut(s) 382, 893
BfoI RGCGCY 1 cut(s) 910
BfuI GTATCC 1 cut(s) 199
BglII AGATCT 2 cut(s) 492, 871
BisI GCNGC 5 cut(s) 6, 82, 336, 765, 986
BlsI GCNGC 5 cut(s) 7, 83, 337, 766, 987
BmeT110I CYCGRG 1 cut(s) 1065
BmiI GGNNCC 6 cut(s) 54, 272, 666, 706, 726, 1213
BmrI ACTGGG 1 cut(s) 1089
BmuI ACTGGG 1 cut(s) 1089
BpmI CTGGAG 1 cut(s) 683
Bpu14I TTCGAA 1 cut(s) 1119
BpuEI CTTGAG 1 cut(s) 567
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 1 cut(s) 700
Bsc4I CCNNNNNNNGG 3 cut(s) 546, 701, 1270
Bse1I ACTGG 2 cut(s) 666, 1095
Bse3DI GCAATG 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 700
BseGI GGATG 4 cut(s) 256, 832, 956, 1174
BseLI CCNNNNNNNGG 3 cut(s) 546, 701, 1270
BseMI GCAATG 1 cut(s) 15
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 2 cut(s) 666, 1095
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 3 cut(s) 347, 776, 972
BseYI CCCAGC 1 cut(s) 1155
BsgI GTGCAG 1 cut(s) 873
Bsh1236I CGCG 1 cut(s) 95
BshNI GGYRCC 1 cut(s) 1211
BsiHKCI CYCGRG 1 cut(s) 1065
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 3 cut(s) 546, 701, 1270
BsmAI GTCTC 4 cut(s) 308, 555, 585, 1037
BsmFI GGGAC 1 cut(s) 139
BsmI GAATGC 1 cut(s) 395
Bso31I GGTCTC 1 cut(s) 308
BsoBI CYCGRG 1 cut(s) 1065
Bsp119I TTCGAA 1 cut(s) 1119
Bsp1286I GDGCHC 1 cut(s) 275
Bsp143I GATC 6 cut(s) 21, 66, 492, 871, 1131, 1260
BspACI CCGC 5 cut(s) 5, 81, 141, 519, 695
BspCNI CTCAG 1 cut(s) 310
BspFNI CGCG 1 cut(s) 95
BspLI GGNNCC 6 cut(s) 54, 272, 666, 706, 726, 1213
BspMAI CTGCAG 1 cut(s) 897
BspPI GGATC 1 cut(s) 1255
BspT104I TTCGAA 1 cut(s) 1119
BspT107I GGYRCC 1 cut(s) 1211
BspTNI GGTCTC 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 15
BsrI ACTGG 2 cut(s) 666, 1095
BssECI CCNNGG 1 cut(s) 700
BssMI GATC 6 cut(s) 21, 66, 492, 871, 1131, 1260
BssSI CACGAG 1 cut(s) 768
BssT1I CCWWGG 1 cut(s) 700
Bst2BI CACGAG 1 cut(s) 768
Bst4CI ACNGT 4 cut(s) 383, 579, 732, 1080
Bst6I CTCTTC 3 cut(s) 347, 444, 1116
BstAPI GCANNNNNTGC 2 cut(s) 536, 770
BstBI TTCGAA 1 cut(s) 1119
BstC8I GCNNGC 1 cut(s) 521
BstDEI CTNAG 1 cut(s) 318
BstF5I GGATG 4 cut(s) 256, 832, 956, 1174
BstFNI CGCG 1 cut(s) 95
BstH2I RGCGCY 1 cut(s) 910
BstHHI GCGC 3 cut(s) 909, 1104, 1207
BstKTI GATC 6 cut(s) 24, 69, 495, 874, 1134, 1263
BstMAI GTCTC 4 cut(s) 308, 555, 585, 1037
BstMBI GATC 6 cut(s) 21, 66, 492, 871, 1131, 1260
BstMWI GCNNNNNNNGC 3 cut(s) 536, 770, 991
BstNSI RCATGY 1 cut(s) 169
BstSFI CTRYAG 2 cut(s) 382, 893
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 3 cut(s) 347, 776, 972
BstX2I RGATCY 2 cut(s) 492, 871
BstYI RGATCY 2 cut(s) 492, 871
BsuI GTATCC 1 cut(s) 199
BtgZI GCGATG 1 cut(s) 45
BtsCI GGATG 4 cut(s) 256, 832, 956, 1174
BtsI GCAGTG 2 cut(s) 319, 890
BtsIMutI CAGTG 6 cut(s) 319, 379, 567, 728, 890, 1102
Cac8I GCNNGC 1 cut(s) 521
CaiI CAGNNNCTG 1 cut(s) 349
CfoI GCGC 3 cut(s) 909, 1104, 1207
Csp6I GTAC 3 cut(s) 41, 957, 1212
CspCI CAANNNNNGTGG 1 cut(s) 32
CviAII CATG 4 cut(s) 166, 777, 1208, 1270
CviQI GTAC 3 cut(s) 41, 957, 1212
DdeI CTNAG 1 cut(s) 318
DpnI GATC 6 cut(s) 23, 68, 494, 873, 1133, 1262
DpnII GATC 6 cut(s) 21, 66, 492, 871, 1131, 1260
DrdI GACNNNNNNGTC 1 cut(s) 944
DseDI GACNNNNNNGTC 1 cut(s) 944
Eam1104I CTCTTC 3 cut(s) 347, 444, 1116
EarI CTCTTC 3 cut(s) 347, 444, 1116
Eco130I CCWWGG 1 cut(s) 700
Eco24I GRGCYC 1 cut(s) 275
Eco31I GGTCTC 1 cut(s) 308
Eco47III AGCGCT 1 cut(s) 908
Eco57I CTGAAG 6 cut(s) 169, 371, 591, 833, 888, 1162
Eco88I CYCGRG 1 cut(s) 1065
EcoT14I CCWWGG 1 cut(s) 700
EcoT38I GRGCYC 1 cut(s) 275
ErhI CCWWGG 1 cut(s) 700
FaeI CATG 4 cut(s) 169, 780, 1211, 1273
FaqI GGGAC 1 cut(s) 139
FatI CATG 4 cut(s) 165, 776, 1207, 1269
FauI CCCGC 2 cut(s) 134, 512
FbaI TGATCA 2 cut(s) 66, 1131
FblI GTMKAC 1 cut(s) 1275
Fnu4HI GCNGC 5 cut(s) 6, 82, 336, 765, 986
FokI GGATG 4 cut(s) 263, 839, 963, 1181
FriOI GRGCYC 1 cut(s) 275
Fsp4HI GCNGC 5 cut(s) 6, 82, 336, 765, 986
FspBI CTAG 3 cut(s) 480, 785, 1008
FspI TGCGCA 1 cut(s) 1206
GlaI GCGC 3 cut(s) 908, 1103, 1206
GluI GCNGC 5 cut(s) 6, 82, 336, 765, 986
GsaI CCCAGC 1 cut(s) 1159
GsuI CTGGAG 1 cut(s) 683
HaeII RGCGCY 1 cut(s) 910
HhaI GCGC 3 cut(s) 909, 1104, 1207
Hin1II CATG 4 cut(s) 169, 780, 1211, 1273
Hin6I GCGC 3 cut(s) 907, 1102, 1205
HinP1I GCGC 3 cut(s) 907, 1102, 1205
HincII GTYRAC 2 cut(s) 408, 1276
HindII GTYRAC 2 cut(s) 408, 1276
HindIII AAGCTT 2 cut(s) 173, 992
HinfI GANTC 3 cut(s) 619, 863, 938
HphI GGTGA 2 cut(s) 307, 467
Hpy166II GTNNAC 4 cut(s) 408, 1174, 1190, 1276
Hpy188I TCNGA 8 cut(s) 351, 489, 852, 868, 950, 1136, 1181, 1246
Hpy188III TCNNGA 4 cut(s) 584, 708, 1008, 1065
Hpy8I GTNNAC 4 cut(s) 408, 1174, 1190, 1276
HpyAV CCTTC 4 cut(s) 205, 540, 817, 851
HpyCH4III ACNGT 4 cut(s) 383, 579, 732, 1080
HpyCH4IV ACGT 3 cut(s) 155, 472, 683
HpyCH4V TGCA 7 cut(s) 335, 393, 530, 890, 895, 923, 1225
HpyF10VI GCNNNNNNNGC 3 cut(s) 536, 770, 991
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 3 cut(s) 155, 472, 683
Hsp92II CATG 4 cut(s) 169, 780, 1211, 1273
HspAI GCGC 3 cut(s) 907, 1102, 1205
KpnI GGTACC 1 cut(s) 1215
Ksp22I TGATCA 2 cut(s) 66, 1131
Kzo9I GATC 6 cut(s) 21, 66, 492, 871, 1131, 1260
LmnI GCTCC 4 cut(s) 52, 111, 270, 664
Lsp1109I GCAGC 3 cut(s) 347, 776, 972
MaeI CTAG 3 cut(s) 480, 785, 1008
MaeII ACGT 3 cut(s) 155, 472, 683
MaeIII GTNAC 2 cut(s) 473, 913
MalI GATC 6 cut(s) 23, 68, 494, 873, 1133, 1262
MboI GATC 6 cut(s) 21, 66, 492, 871, 1131, 1260
MflI RGATCY 2 cut(s) 492, 871
MhlI GDGCHC 1 cut(s) 275
MluCI AATT 8 cut(s) 322, 651, 673, 878, 1047, 1073, 1110, 1279
MlyI GAGTC 1 cut(s) 932
MmeI TCCRAC 3 cut(s) 928, 1062, 1288
MseI TTAA 3 cut(s) 812, 876, 996
MslI CAYNNNNRTG 3 cut(s) 384, 480, 1220
Mva1269I GAATGC 1 cut(s) 395
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 3 cut(s) 536, 770, 991
NdeII GATC 6 cut(s) 21, 66, 492, 871, 1131, 1260
NlaIII CATG 4 cut(s) 169, 780, 1211, 1273
NlaIV GGNNCC 6 cut(s) 54, 272, 666, 706, 726, 1213
NmuCI GTSAC 1 cut(s) 473
NsbI TGCGCA 1 cut(s) 1206
NspI RCATGY 1 cut(s) 169
NspV TTCGAA 1 cut(s) 1119
OliI CACNNNNGTG 2 cut(s) 384, 480
PctI GAATGC 1 cut(s) 395
PfeI GAWTC 2 cut(s) 619, 863
PflMI CCANNNNNTGG 1 cut(s) 1270
PkrI GCNGC 5 cut(s) 7, 83, 337, 766, 987
PleI GAGTC 1 cut(s) 932
PpsI GAGTC 1 cut(s) 932
PsiI TTATAA 2 cut(s) 680, 1034
Psp1406I AACGTT 1 cut(s) 155
PspFI CCCAGC 1 cut(s) 1155
PspN4I GGNNCC 6 cut(s) 54, 272, 666, 706, 726, 1213
PstI CTGCAG 1 cut(s) 897
PstNI CAGNNNCTG 1 cut(s) 349
PsuI RGATCY 2 cut(s) 492, 871
RsaI GTAC 3 cut(s) 42, 958, 1213
RsaNI GTAC 3 cut(s) 41, 957, 1212
RseI CAYNNNNRTG 3 cut(s) 384, 480, 1220
SalI GTCGAC 1 cut(s) 1274
SaqAI TTAA 3 cut(s) 812, 876, 996
SatI GCNGC 5 cut(s) 6, 82, 336, 765, 986
Sau3AI GATC 6 cut(s) 21, 66, 492, 871, 1131, 1260
SchI GAGTC 1 cut(s) 932
SduI GDGCHC 1 cut(s) 275
SfcI CTRYAG 2 cut(s) 382, 893
SfuI TTCGAA 1 cut(s) 1119
SmiMI CAYNNNNRTG 3 cut(s) 384, 480, 1220
SmlI CTYRAG 1 cut(s) 582
SmoI CTYRAG 1 cut(s) 582
Sse9I AATT 8 cut(s) 322, 651, 673, 878, 1047, 1073, 1110, 1279
SsiI CCGC 5 cut(s) 5, 81, 141, 519, 695
SspMI CTAG 3 cut(s) 480, 785, 1008
StyI CCWWGG 1 cut(s) 700
TaaI ACNGT 4 cut(s) 383, 579, 732, 1080
TaiI ACGT 3 cut(s) 158, 475, 686
TaqI TCGA 4 cut(s) 191, 234, 1119, 1275
TasI AATT 8 cut(s) 322, 651, 673, 878, 1047, 1073, 1110, 1279
TatI WGTACW 1 cut(s) 956
TauI GCSGC 2 cut(s) 8, 84
TfiI GAWTC 2 cut(s) 619, 863
Tru1I TTAA 3 cut(s) 812, 876, 996
Tru9I TTAA 3 cut(s) 812, 876, 996
TscAI CASTG 6 cut(s) 319, 386, 574, 735, 897, 1102
TseFI GTSAC 1 cut(s) 473
TseI GCWGC 3 cut(s) 335, 764, 985
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 4 cut(s) 267, 570, 765, 871
TspGWI ACGGA 2 cut(s) 218, 612
TspRI CASTG 6 cut(s) 319, 386, 574, 735, 897, 1102
Van91I CCANNNNNTGG 1 cut(s) 1270
XapI RAATTY 2 cut(s) 673, 878
XbaI TCTAGA 1 cut(s) 1007
XceI RCATGY 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 1275
XspI CTAG 3 cut(s) 480, 785, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.