RchiOBHm_Chr6g0306871

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008052
Physical Location & Seq
Forward (+)
65377354 .. 65381129
3776 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27589

Sequence Viewer

Length: 1068 bp
ATGGCTTCGCTGGAGTCGGAGGCCGGCTCCAAATCTTCCAATGAAATCAAACAGAGAGCCAGAAAAAATAGGACCAAGAAGAAGAAGACCAAAAACGGTGCTGCCGAGGATTGGAGGTATGCAGCGACCCAGTTTGTGGAGAGATACCCAGAAGATGTTGTTGTGCATTGCAGTGAATGTAATTGTTCAACGTTGACATTTGATGGTGTGGATGTAATGGGAGAGAGATTAGCAGAAGAGGTTAAATCTGTAATAAAACGCCACCCAAGTGTTCAGAAGATCTCGTTTGTTGGTCACTCATTAGGTGGCCTTATAGCAAGGTATGCCATTGGCAGGCTTTATGAACGCGACACAACAAGGGAACTTTCTCTGGAAAATGGAGAATGTAGGGGCAATGGATTTGAGGATCCATCGCCCGAACAGAAAGCGAAAAGCAAAATTGCTGGGCTGGAGCCGGTGAATTTTATAACATCTGCAACTCCGCACCTCGGTTCATGGGGGCATAAACAGGTTCCAGTGTTTTGTGGCTTTCAAATCCTTGAAAAAGCAGCAGCTAGTACTTCATGGTGTTTAGGTAGAACTGGAAGACATCTGTTTTTAACTGATAGGGATAAAGGAAAACCTCCCCTTTTACTTCAGATGCTTTATGATTCTGAAGATCTTAAATTCTTATCTGCATTGAAGTCCTTCAGGCGTCATGTTGCCTATGCAAATGTTCATTATGATCACCTCGTGGGATGGAGTACGTCTTCTATACGTCGTAAGAAGGAGCTGCCAAAGCTTAAACATTTATCCAGAGACAACAAATATCCGCATATTGTAAATGAGGACATGATAAACACCGCTAGTACTCAAGAAGAAGTTGCTTTGGAGGACAACGTGAATATTGACTTCGAAGAGGAAATGATCAGAGGTTTGACCAAGGTGAGCTGGGAAAGGATTGATGTAAGTTTTAGTGGAAGTAGACAAAAGTACCTTGCACATAATACCATTCAGGTGAAAACTTATTGTATACATTCTGATGGAGCTGATGTGATCCAACACATGGTGGACAATTTTCGTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

355

Amino Acids

40.25

Weight (kDa)

8.69

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 33 - 249 1.7e-57 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 467
AccB7I CCANNNNNTGG 2 cut(s) 136, 1045
AccI GTMKAC 2 cut(s) 964, 1012
AccII CGCG 1 cut(s) 348
AciI CCGC 3 cut(s) 482, 812, 843
AclI AACGTT 1 cut(s) 191
AclWI GGATC 3 cut(s) 401, 414, 1030
AcsI RAATTY 2 cut(s) 460, 665
AcuI CTGAAG 3 cut(s) 620, 673, 675
AcyI GRCGYC 1 cut(s) 694
AdeI CACNNNGTG 2 cut(s) 733, 1048
AfaI GTAC 4 cut(s) 559, 745, 850, 974
AfiI CCNNNNNNNGG 5 cut(s) 111, 136, 333, 488, 1045
AgsI TTSAA 4 cut(s) 189, 533, 542, 682
AleI CACNNNNGTG 1 cut(s) 267
AluBI AGCT 5 cut(s) 554, 772, 781, 930, 1028
AluI AGCT 5 cut(s) 554, 772, 781, 930, 1028
Alw26I GTCTC 1 cut(s) 792
AlwI GGATC 3 cut(s) 401, 414, 1030
AoxI GGCC 2 cut(s) 21, 307
ApeKI GCWGC 5 cut(s) 101, 122, 548, 551, 772
ApoI RAATTY 2 cut(s) 460, 665
Asp700I GAANNNNTTC 1 cut(s) 686
AspS9I GGNCC 1 cut(s) 72
AsuHPI GGTGA 4 cut(s) 469, 719, 937, 1009
AsuII TTCGAA 1 cut(s) 894
AvaII GGWCC 1 cut(s) 72
BaeI ACNNNNGTAYC 2 cut(s) 956, 989
BamHI GGATCC 1 cut(s) 406
BauI CACGAG 1 cut(s) 731
BbsI GAAGAC 3 cut(s) 92, 592, 741
BbvI GCAGC 5 cut(s) 88, 134, 560, 563, 759
BccI CCATC 4 cut(s) 197, 418, 732, 1016
BclI TGATCA 2 cut(s) 724, 906
BcoDI GTCTC 1 cut(s) 792
BfaI CTAG 2 cut(s) 555, 846
BglII AGATCT 2 cut(s) 279, 658
BisI GCNGC 5 cut(s) 102, 123, 549, 552, 773
BlsI GCNGC 5 cut(s) 103, 124, 550, 553, 774
BmcAI AGTACT 2 cut(s) 559, 850
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 4 cut(s) 28, 408, 453, 513
BmrI ACTGGG 1 cut(s) 124
BmsI GCATC 1 cut(s) 630
BmuI ACTGGG 1 cut(s) 124
BpiI GAAGAC 3 cut(s) 92, 592, 741
BplI GAGNNNNNCTC 2 cut(s) 11, 43
BpmI CTGGAG 2 cut(s) 32, 470
Bpu14I TTCGAA 1 cut(s) 894
BpuEI CTTGAG 1 cut(s) 837
BsaHI GRCGYC 1 cut(s) 694
BsaJI CCNNGG 3 cut(s) 105, 487, 921
BsaXI ACNNNNNCTCC 2 cut(s) 1017, 1047
Bsc4I CCNNNNNNNGG 5 cut(s) 111, 136, 333, 488, 1045
Bse118I RCCGGY 2 cut(s) 23, 454
Bse1I ACTGG 3 cut(s) 130, 515, 586
Bse3DI GCAATG 2 cut(s) 166, 400
BseDI CCNNGG 3 cut(s) 105, 487, 921
BseGI GGATG 2 cut(s) 217, 743
BseLI CCNNNNNNNGG 5 cut(s) 111, 136, 333, 488, 1045
BseMI GCAATG 2 cut(s) 166, 400
BseNI ACTGG 3 cut(s) 130, 515, 586
BseXI GCAGC 5 cut(s) 88, 134, 560, 563, 759
BseYI CCCAGC 2 cut(s) 443, 930
Bsh1236I CGCG 1 cut(s) 348
BshFI GGCC 2 cut(s) 23, 309
BsiSI CCGG 2 cut(s) 24, 455
BslI CCNNNNNNNGG 5 cut(s) 111, 136, 333, 488, 1045
BsmAI GTCTC 1 cut(s) 792
BsnI GGCC 2 cut(s) 23, 309
Bsp119I TTCGAA 1 cut(s) 894
Bsp143I GATC 6 cut(s) 279, 406, 658, 724, 906, 1035
BspACI CCGC 3 cut(s) 482, 812, 843
BspANI GGCC 2 cut(s) 23, 309
BspFNI CGCG 1 cut(s) 348
BspLI GGNNCC 4 cut(s) 28, 408, 453, 513
BspPI GGATC 3 cut(s) 401, 414, 1030
BspT104I TTCGAA 1 cut(s) 894
BsrDI GCAATG 2 cut(s) 166, 400
BsrFI RCCGGY 2 cut(s) 23, 454
BsrI ACTGG 3 cut(s) 130, 515, 586
BssAI RCCGGY 2 cut(s) 23, 454
BssECI CCNNGG 3 cut(s) 105, 487, 921
BssMI GATC 6 cut(s) 279, 406, 658, 724, 906, 1035
BssNAI GTATAC 1 cut(s) 1013
BssNI GRCGYC 1 cut(s) 694
BssSI CACGAG 1 cut(s) 731
BssT1I CCWWGG 1 cut(s) 921
Bst1107I GTATAC 1 cut(s) 1013
Bst2BI CACGAG 1 cut(s) 731
Bst4CI ACNGT 1 cut(s) 98
Bst6I CTCTTC 2 cut(s) 231, 891
BstACI GRCGYC 1 cut(s) 694
BstAPI GCANNNNNTGC 1 cut(s) 323
BstBI TTCGAA 1 cut(s) 894
BstC8I GCNNGC 2 cut(s) 25, 335
BstF5I GGATG 2 cut(s) 217, 743
BstFNI CGCG 1 cut(s) 348
BstKTI GATC 6 cut(s) 282, 409, 661, 727, 909, 1038
BstMAI GTCTC 1 cut(s) 792
BstMBI GATC 6 cut(s) 279, 406, 658, 724, 906, 1035
BstMWI GCNNNNNNNGC 2 cut(s) 323, 778
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 5 cut(s) 88, 134, 560, 563, 759
BstV2I GAAGAC 3 cut(s) 92, 592, 741
BstX2I RGATCY 3 cut(s) 279, 406, 658
BstYI RGATCY 3 cut(s) 279, 406, 658
BstZ17I GTATAC 1 cut(s) 1013
BsuRI GGCC 2 cut(s) 23, 309
BtgZI GCGATG 1 cut(s) 396
BtsCI GGATG 2 cut(s) 217, 743
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 2 cut(s) 178, 522
Cac8I GCNNGC 2 cut(s) 25, 335
Cfr10I RCCGGY 2 cut(s) 23, 454
Cfr13I GGNCC 1 cut(s) 72
CseI GACGC 1 cut(s) 683
Csp6I GTAC 4 cut(s) 558, 744, 849, 973
CviAII CATG 5 cut(s) 495, 564, 698, 832, 1045
CviQI GTAC 4 cut(s) 558, 744, 849, 973
DpnI GATC 6 cut(s) 281, 408, 660, 726, 908, 1037
DpnII GATC 6 cut(s) 279, 406, 658, 724, 906, 1035
DraIII CACNNNGTG 2 cut(s) 733, 1048
Eam1104I CTCTTC 2 cut(s) 231, 891
EarI CTCTTC 2 cut(s) 231, 891
Eco130I CCWWGG 1 cut(s) 921
Eco47I GGWCC 1 cut(s) 72
Eco57I CTGAAG 3 cut(s) 620, 673, 675
EcoT14I CCWWGG 1 cut(s) 921
ErhI CCWWGG 1 cut(s) 921
FaeI CATG 5 cut(s) 498, 567, 701, 835, 1048
FatI CATG 5 cut(s) 494, 563, 697, 831, 1044
FbaI TGATCA 2 cut(s) 724, 906
FblI GTMKAC 2 cut(s) 964, 1012
Fnu4HI GCNGC 5 cut(s) 102, 123, 549, 552, 773
FokI GGATG 2 cut(s) 224, 750
Fsp4HI GCNGC 5 cut(s) 102, 123, 549, 552, 773
FspBI CTAG 2 cut(s) 555, 846
GluI GCNGC 5 cut(s) 102, 123, 549, 552, 773
GsaI CCCAGC 2 cut(s) 447, 934
GsuI CTGGAG 2 cut(s) 32, 470
HaeIII GGCC 2 cut(s) 23, 309
HapII CCGG 2 cut(s) 24, 455
HgaI GACGC 1 cut(s) 683
Hin1I GRCGYC 1 cut(s) 694
Hin1II CATG 5 cut(s) 498, 567, 701, 835, 1048
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HindIII AAGCTT 1 cut(s) 779
HinfI GANTC 2 cut(s) 14, 650
HpaII CCGG 2 cut(s) 24, 455
HphI GGTGA 4 cut(s) 469, 719, 937, 1009
Hpy166II GTNNAC 4 cut(s) 195, 965, 1013, 1051
Hpy188I TCNGA 6 cut(s) 19, 276, 639, 655, 911, 1021
Hpy188III TCNNGA 3 cut(s) 371, 795, 854
Hpy8I GTNNAC 4 cut(s) 195, 965, 1013, 1051
Hpy99I CGWCG 1 cut(s) 762
HpyAV CCTTC 2 cut(s) 697, 760
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4IV ACGT 4 cut(s) 191, 746, 757, 879
HpyCH4V TGCA 7 cut(s) 122, 166, 171, 476, 677, 710, 980
HpyF10VI GCNNNNNNNGC 2 cut(s) 323, 778
HpySE526I ACGT 4 cut(s) 191, 746, 757, 879
Hsp92I GRCGYC 1 cut(s) 694
Hsp92II CATG 5 cut(s) 498, 567, 701, 835, 1048
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Ksp22I TGATCA 2 cut(s) 724, 906
Kzo9I GATC 6 cut(s) 279, 406, 658, 724, 906, 1035
LmnI GCTCC 4 cut(s) 32, 451, 769, 1025
Lsp1109I GCAGC 5 cut(s) 88, 134, 560, 563, 759
LweI GCATC 1 cut(s) 630
MaeI CTAG 2 cut(s) 555, 846
MaeII ACGT 4 cut(s) 191, 746, 757, 879
MaeIII GTNAC 1 cut(s) 293
MalI GATC 6 cut(s) 281, 408, 660, 726, 908, 1037
MboI GATC 6 cut(s) 279, 406, 658, 724, 906, 1035
MflI RGATCY 3 cut(s) 279, 406, 658
MluCI AATT 5 cut(s) 181, 438, 460, 665, 1054
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 1063
MroNI GCCGGC 1 cut(s) 23
MroXI GAANNNNTTC 1 cut(s) 686
MseI TTAA 4 cut(s) 243, 599, 663, 783
MslI CAYNNNNRTG 4 cut(s) 171, 267, 995, 1020
MspI CCGG 2 cut(s) 24, 455
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 2 cut(s) 323, 778
NaeI GCCGGC 1 cut(s) 25
NdeII GATC 6 cut(s) 279, 406, 658, 724, 906, 1035
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 5 cut(s) 498, 567, 701, 835, 1048
NlaIV GGNNCC 4 cut(s) 28, 408, 453, 513
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 293
NspV TTCGAA 1 cut(s) 894
OliI CACNNNNGTG 1 cut(s) 267
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdiI GCCGGC 1 cut(s) 25
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 1 cut(s) 650
PflMI CCANNNNNTGG 2 cut(s) 136, 1045
PkrI GCNGC 5 cut(s) 103, 124, 550, 553, 774
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
PsiI TTATAA 1 cut(s) 467
Psp1406I AACGTT 1 cut(s) 191
PspFI CCCAGC 2 cut(s) 443, 930
PspN4I GGNNCC 4 cut(s) 28, 408, 453, 513
PspPI GGNCC 1 cut(s) 72
PsuI RGATCY 3 cut(s) 279, 406, 658
RsaI GTAC 4 cut(s) 559, 745, 850, 974
RsaNI GTAC 4 cut(s) 558, 744, 849, 973
RseI CAYNNNNRTG 4 cut(s) 171, 267, 995, 1020
SaqAI TTAA 4 cut(s) 243, 599, 663, 783
SatI GCNGC 5 cut(s) 102, 123, 549, 552, 773
Sau3AI GATC 6 cut(s) 279, 406, 658, 724, 906, 1035
Sau96I GGNCC 1 cut(s) 72
ScaI AGTACT 2 cut(s) 559, 850
SchI GAGTC 1 cut(s) 23
SfaNI GCATC 1 cut(s) 630
SfuI TTCGAA 1 cut(s) 894
SinI GGWCC 1 cut(s) 72
SmiMI CAYNNNNRTG 4 cut(s) 171, 267, 995, 1020
SmlI CTYRAG 1 cut(s) 852
SmoI CTYRAG 1 cut(s) 852
Sse9I AATT 5 cut(s) 181, 438, 460, 665, 1054
SsiI CCGC 3 cut(s) 482, 812, 843
SspI AATATT 1 cut(s) 886
SspMI CTAG 2 cut(s) 555, 846
StyI CCWWGG 1 cut(s) 921
TaaI ACNGT 1 cut(s) 98
TaiI ACGT 4 cut(s) 194, 749, 760, 882
TaqI TCGA 1 cut(s) 894
TasI AATT 5 cut(s) 181, 438, 460, 665, 1054
TatI WGTACW 2 cut(s) 557, 848
TfiI GAWTC 1 cut(s) 650
Tru1I TTAA 4 cut(s) 243, 599, 663, 783
Tru9I TTAA 4 cut(s) 243, 599, 663, 783
TscAI CASTG 2 cut(s) 178, 522
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 5 cut(s) 101, 122, 548, 551, 772
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 5 cut(s) 57, 357, 483, 552, 707
TspRI CASTG 2 cut(s) 178, 522
Van91I CCANNNNNTGG 2 cut(s) 136, 1045
VpaK11BI GGWCC 1 cut(s) 72
XapI RAATTY 2 cut(s) 460, 665
XmiI GTMKAC 2 cut(s) 964, 1012
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 2 cut(s) 555, 846
ZrmI AGTACT 2 cut(s) 559, 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.