Rh6AG465500

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0008052
Physical Location & Seq
Forward (+)
64739163 .. 64742448
3286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG465500.1

Sequence Viewer

Length: 1218 bp
ATGGCTTCGCTGGAGTCGGAGGCCGGCTCCAAATCTTCCAATGAAATCAAACAGAGAGCCAGAAAAAATAGGACCAAGAAGAAGAAGACCAAAAACGGTGCGTTTCGTTACCTCCCCAAATTTCGGTGCTTTAGTTTAAAATCGGAAACGACTGACGGGAGTTTCGATATGGATATGGAGGTGGAGGATGAAGCGGGTCGGGGAATGAACCCGACCCACCTTGTTATAATGGTTAATGGGCTTATAGGCAGTGCCGAGGATTGGAGGTATGCAGCGACCCAGTTTGTGGAGAGATACCCAGAAGATGTTGTTGTGCATTGCAGTGAATGTAATTGTTCAACGTTGACATTTGATGGTGTGGATGTAATGGGAGAGAGATTAGCAGAAGAGGTTAAATCTGTAATAAAACGCCACCCAAGTGTTCAGAAGATCTCGTTTGTTGGTCACTCATTAGGTGGCCTTATAGCAAGGTATGCCATTGGCAGGCTTTATGAACGCGACACAACAAGGGAACTTTCTCTGGAAAATGGAGAATGTAGGGGCAATGGATTTGAGGATCCATCGCCCGAACAGAAAGCGAAAAGCAAAATTGCTGGGCTGGAGCCGGTGAATTTTATAACATCTGCAACTCCGCACCTCGGTTCATGGGGGCATAAACAGGTTCCAGTGTTTTGTGGCTTTCAAATCCTTGAAAAAGCAGCAGCTAGTACTTCATGGTGTTTAGGTAGAACTGGAAGACATCTGTTTTTAACTGATAGGGATAAAGGAAAACCTCCCCTTTTACTTCAGATGCTTTATGATTCTGAAGATCTTAAATTCTTATCTGCATTGAAGTCCTTCAGGCGTCATGTTGCCTATGCAAATGTTCATTATGATCACCTCGTGGGATGGAGTACGTCTTCTATACGTCGTAAGAAGGAGCTGCCAAAGCTTAAACATTTATCCAGAGACAACAAATATCCGCATATTGTAAATGAGGACATGATAAACACCGCTAGTACTCAAGAAGAAGTTGCTTTGGAGGACAACGTGAATATTGACTTCGAAGAGGAAATGATCAGAGGTTTGACCAAGGTGAGCTGGGAAAGGATTGATGTAAGTTTTAGTGGAAGTAGACAAAAGTACCTTGCACATAATACCATTCAGGTGAAAACTTATTGTATACATTCTGATGGAGCTGATGTGATCCAACACATGGTGGACAATTTTCGTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

405

Amino Acids

45.87

Weight (kDa)

8.04

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 70 - 299 3.1e-65 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 227, 617
AccB7I CCANNNNNTGG 2 cut(s) 286, 1195
AccI GTMKAC 2 cut(s) 1114, 1162
AccII CGCG 1 cut(s) 498
AciI CCGC 4 cut(s) 194, 632, 962, 993
AclI AACGTT 1 cut(s) 341
AclWI GGATC 3 cut(s) 551, 564, 1180
AcsI RAATTY 3 cut(s) 119, 610, 815
AcuI CTGAAG 3 cut(s) 770, 823, 825
AcyI GRCGYC 1 cut(s) 844
AdeI CACNNNGTG 2 cut(s) 883, 1198
AfaI GTAC 4 cut(s) 709, 895, 1000, 1124
AfiI CCNNNNNNNGG 6 cut(s) 123, 261, 286, 483, 638, 1195
AgsI TTSAA 4 cut(s) 339, 683, 692, 832
AleI CACNNNNGTG 1 cut(s) 417
AluBI AGCT 5 cut(s) 704, 922, 931, 1080, 1178
AluI AGCT 5 cut(s) 704, 922, 931, 1080, 1178
Alw26I GTCTC 1 cut(s) 942
AlwI GGATC 3 cut(s) 551, 564, 1180
AoxI GGCC 2 cut(s) 21, 457
ApeKI GCWGC 4 cut(s) 272, 698, 701, 922
ApoI RAATTY 3 cut(s) 119, 610, 815
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
Asp700I GAANNNNTTC 1 cut(s) 836
AspS9I GGNCC 1 cut(s) 72
AsuHPI GGTGA 4 cut(s) 619, 869, 1087, 1159
AsuII TTCGAA 1 cut(s) 1044
AvaII GGWCC 1 cut(s) 72
BaeI ACNNNNGTAYC 2 cut(s) 1106, 1139
BamHI GGATCC 1 cut(s) 556
BauI CACGAG 1 cut(s) 881
BbsI GAAGAC 3 cut(s) 92, 742, 891
BbvI GCAGC 4 cut(s) 284, 710, 713, 909
BccI CCATC 4 cut(s) 347, 568, 882, 1166
BclI TGATCA 2 cut(s) 874, 1056
BcoDI GTCTC 1 cut(s) 942
BfaI CTAG 2 cut(s) 705, 996
BglII AGATCT 2 cut(s) 429, 808
BisI GCNGC 4 cut(s) 273, 699, 702, 923
BlsI GCNGC 4 cut(s) 274, 700, 703, 924
BmcAI AGTACT 2 cut(s) 709, 1000
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 4 cut(s) 28, 558, 603, 663
BmrI ACTGGG 1 cut(s) 274
BmsI GCATC 1 cut(s) 780
BmuI ACTGGG 1 cut(s) 274
BpiI GAAGAC 3 cut(s) 92, 742, 891
BplI GAGNNNNNCTC 2 cut(s) 11, 43
BpmI CTGGAG 2 cut(s) 32, 620
Bpu14I TTCGAA 1 cut(s) 1044
BpuEI CTTGAG 1 cut(s) 987
BsaHI GRCGYC 1 cut(s) 844
BsaJI CCNNGG 3 cut(s) 255, 637, 1071
BsaXI ACNNNNNCTCC 2 cut(s) 1167, 1197
Bsc4I CCNNNNNNNGG 6 cut(s) 123, 261, 286, 483, 638, 1195
Bse118I RCCGGY 2 cut(s) 23, 604
Bse1I ACTGG 3 cut(s) 280, 665, 736
Bse3DI GCAATG 2 cut(s) 316, 550
BseDI CCNNGG 3 cut(s) 255, 637, 1071
BseGI GGATG 3 cut(s) 193, 367, 893
BseLI CCNNNNNNNGG 6 cut(s) 123, 261, 286, 483, 638, 1195
BseMI GCAATG 2 cut(s) 316, 550
BseNI ACTGG 3 cut(s) 280, 665, 736
BseXI GCAGC 4 cut(s) 284, 710, 713, 909
BseYI CCCAGC 2 cut(s) 593, 1080
Bsh1236I CGCG 1 cut(s) 498
BshFI GGCC 2 cut(s) 23, 459
BsiSI CCGG 2 cut(s) 24, 605
BslI CCNNNNNNNGG 6 cut(s) 123, 261, 286, 483, 638, 1195
BsmAI GTCTC 1 cut(s) 942
BsnI GGCC 2 cut(s) 23, 459
Bsp119I TTCGAA 1 cut(s) 1044
Bsp143I GATC 6 cut(s) 429, 556, 808, 874, 1056, 1185
BspACI CCGC 4 cut(s) 194, 632, 962, 993
BspANI GGCC 2 cut(s) 23, 459
BspFNI CGCG 1 cut(s) 498
BspLI GGNNCC 4 cut(s) 28, 558, 603, 663
BspPI GGATC 3 cut(s) 551, 564, 1180
BspT104I TTCGAA 1 cut(s) 1044
BsrDI GCAATG 2 cut(s) 316, 550
BsrFI RCCGGY 2 cut(s) 23, 604
BsrI ACTGG 3 cut(s) 280, 665, 736
BssAI RCCGGY 2 cut(s) 23, 604
BssECI CCNNGG 3 cut(s) 255, 637, 1071
BssMI GATC 6 cut(s) 429, 556, 808, 874, 1056, 1185
BssNAI GTATAC 1 cut(s) 1163
BssNI GRCGYC 1 cut(s) 844
BssSI CACGAG 1 cut(s) 881
BssT1I CCWWGG 1 cut(s) 1071
Bst1107I GTATAC 1 cut(s) 1163
Bst2BI CACGAG 1 cut(s) 881
Bst4CI ACNGT 1 cut(s) 98
Bst6I CTCTTC 2 cut(s) 381, 1041
BstACI GRCGYC 1 cut(s) 844
BstAPI GCANNNNNTGC 1 cut(s) 473
BstBI TTCGAA 1 cut(s) 1044
BstC8I GCNNGC 2 cut(s) 25, 485
BstF5I GGATG 3 cut(s) 193, 367, 893
BstFNI CGCG 1 cut(s) 498
BstKTI GATC 6 cut(s) 432, 559, 811, 877, 1059, 1188
BstMAI GTCTC 1 cut(s) 942
BstMBI GATC 6 cut(s) 429, 556, 808, 874, 1056, 1185
BstMWI GCNNNNNNNGC 2 cut(s) 473, 928
BstUI CGCG 1 cut(s) 498
BstV1I GCAGC 4 cut(s) 284, 710, 713, 909
BstV2I GAAGAC 3 cut(s) 92, 742, 891
BstX2I RGATCY 3 cut(s) 429, 556, 808
BstYI RGATCY 3 cut(s) 429, 556, 808
BstZ17I GTATAC 1 cut(s) 1163
BsuRI GGCC 2 cut(s) 23, 459
BtgZI GCGATG 1 cut(s) 546
BtsCI GGATG 3 cut(s) 193, 367, 893
BtsI GCAGTG 2 cut(s) 256, 328
BtsIMutI CAGTG 3 cut(s) 256, 328, 672
Cac8I GCNNGC 2 cut(s) 25, 485
Cfr10I RCCGGY 2 cut(s) 23, 604
Cfr13I GGNCC 1 cut(s) 72
CseI GACGC 1 cut(s) 833
Csp6I GTAC 4 cut(s) 708, 894, 999, 1123
CviAII CATG 5 cut(s) 645, 714, 848, 982, 1195
CviQI GTAC 4 cut(s) 708, 894, 999, 1123
DpnI GATC 6 cut(s) 431, 558, 810, 876, 1058, 1187
DpnII GATC 6 cut(s) 429, 556, 808, 874, 1056, 1185
DraI TTTAAA 1 cut(s) 138
DraIII CACNNNGTG 2 cut(s) 883, 1198
Eam1104I CTCTTC 2 cut(s) 381, 1041
EarI CTCTTC 2 cut(s) 381, 1041
Eco130I CCWWGG 1 cut(s) 1071
Eco47I GGWCC 1 cut(s) 72
Eco57I CTGAAG 3 cut(s) 770, 823, 825
EcoT14I CCWWGG 1 cut(s) 1071
ErhI CCWWGG 1 cut(s) 1071
FaeI CATG 5 cut(s) 648, 717, 851, 985, 1198
FatI CATG 5 cut(s) 644, 713, 847, 981, 1194
FauI CCCGC 1 cut(s) 187
FbaI TGATCA 2 cut(s) 874, 1056
FblI GTMKAC 2 cut(s) 1114, 1162
Fnu4HI GCNGC 4 cut(s) 273, 699, 702, 923
FokI GGATG 3 cut(s) 200, 374, 900
Fsp4HI GCNGC 4 cut(s) 273, 699, 702, 923
FspBI CTAG 2 cut(s) 705, 996
GluI GCNGC 4 cut(s) 273, 699, 702, 923
GsaI CCCAGC 2 cut(s) 597, 1084
GsuI CTGGAG 2 cut(s) 32, 620
HaeIII GGCC 2 cut(s) 23, 459
HapII CCGG 2 cut(s) 24, 605
HgaI GACGC 1 cut(s) 833
Hin1I GRCGYC 1 cut(s) 844
Hin1II CATG 5 cut(s) 648, 717, 851, 985, 1198
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 929
HinfI GANTC 2 cut(s) 14, 800
HpaII CCGG 2 cut(s) 24, 605
HphI GGTGA 4 cut(s) 619, 869, 1087, 1159
Hpy166II GTNNAC 4 cut(s) 345, 1115, 1163, 1201
Hpy188I TCNGA 7 cut(s) 19, 145, 426, 789, 805, 1061, 1171
Hpy188III TCNNGA 3 cut(s) 521, 945, 1004
Hpy8I GTNNAC 4 cut(s) 345, 1115, 1163, 1201
Hpy99I CGWCG 1 cut(s) 912
HpyAV CCTTC 2 cut(s) 847, 910
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4IV ACGT 4 cut(s) 341, 896, 907, 1029
HpyCH4V TGCA 7 cut(s) 272, 316, 321, 626, 827, 860, 1130
HpyF10VI GCNNNNNNNGC 2 cut(s) 473, 928
HpySE526I ACGT 4 cut(s) 341, 896, 907, 1029
Hsp92I GRCGYC 1 cut(s) 844
Hsp92II CATG 5 cut(s) 648, 717, 851, 985, 1198
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Ksp22I TGATCA 2 cut(s) 874, 1056
Kzo9I GATC 6 cut(s) 429, 556, 808, 874, 1056, 1185
LmnI GCTCC 4 cut(s) 32, 601, 919, 1175
Lsp1109I GCAGC 4 cut(s) 284, 710, 713, 909
LweI GCATC 1 cut(s) 780
MaeI CTAG 2 cut(s) 705, 996
MaeII ACGT 4 cut(s) 341, 896, 907, 1029
MaeIII GTNAC 2 cut(s) 107, 443
MalI GATC 6 cut(s) 431, 558, 810, 876, 1058, 1187
MboI GATC 6 cut(s) 429, 556, 808, 874, 1056, 1185
MflI RGATCY 3 cut(s) 429, 556, 808
MluCI AATT 6 cut(s) 119, 331, 588, 610, 815, 1204
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 1213
MroNI GCCGGC 1 cut(s) 23
MroXI GAANNNNTTC 1 cut(s) 836
MseI TTAA 6 cut(s) 137, 234, 393, 749, 813, 933
MslI CAYNNNNRTG 4 cut(s) 321, 417, 1145, 1170
MspI CCGG 2 cut(s) 24, 605
MvnI CGCG 1 cut(s) 498
MwoI GCNNNNNNNGC 2 cut(s) 473, 928
NaeI GCCGGC 1 cut(s) 25
NdeII GATC 6 cut(s) 429, 556, 808, 874, 1056, 1185
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 5 cut(s) 648, 717, 851, 985, 1198
NlaIV GGNNCC 4 cut(s) 28, 558, 603, 663
NmeAIII GCCGAG 1 cut(s) 280
NmuCI GTSAC 1 cut(s) 443
NspV TTCGAA 1 cut(s) 1044
OliI CACNNNNGTG 1 cut(s) 417
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PdiI GCCGGC 1 cut(s) 25
PdmI GAANNNNTTC 1 cut(s) 836
PfeI GAWTC 1 cut(s) 800
PflMI CCANNNNNTGG 2 cut(s) 286, 1195
PkrI GCNGC 4 cut(s) 274, 700, 703, 924
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
PsiI TTATAA 2 cut(s) 227, 617
Psp1406I AACGTT 1 cut(s) 341
PspFI CCCAGC 2 cut(s) 593, 1080
PspN4I GGNNCC 4 cut(s) 28, 558, 603, 663
PspPI GGNCC 1 cut(s) 72
PsuI RGATCY 3 cut(s) 429, 556, 808
RsaI GTAC 4 cut(s) 709, 895, 1000, 1124
RsaNI GTAC 4 cut(s) 708, 894, 999, 1123
RseI CAYNNNNRTG 4 cut(s) 321, 417, 1145, 1170
SaqAI TTAA 6 cut(s) 137, 234, 393, 749, 813, 933
SatI GCNGC 4 cut(s) 273, 699, 702, 923
Sau3AI GATC 6 cut(s) 429, 556, 808, 874, 1056, 1185
Sau96I GGNCC 1 cut(s) 72
ScaI AGTACT 2 cut(s) 709, 1000
SchI GAGTC 1 cut(s) 23
SfaNI GCATC 1 cut(s) 780
SfuI TTCGAA 1 cut(s) 1044
SinI GGWCC 1 cut(s) 72
SmiMI CAYNNNNRTG 4 cut(s) 321, 417, 1145, 1170
SmlI CTYRAG 1 cut(s) 1002
SmoI CTYRAG 1 cut(s) 1002
Sse9I AATT 6 cut(s) 119, 331, 588, 610, 815, 1204
SsiI CCGC 4 cut(s) 194, 632, 962, 993
SspI AATATT 1 cut(s) 1036
SspMI CTAG 2 cut(s) 705, 996
StyI CCWWGG 1 cut(s) 1071
TaaI ACNGT 1 cut(s) 98
TaiI ACGT 4 cut(s) 344, 899, 910, 1032
TaqI TCGA 2 cut(s) 165, 1044
TasI AATT 6 cut(s) 119, 331, 588, 610, 815, 1204
TatI WGTACW 2 cut(s) 707, 998
TfiI GAWTC 1 cut(s) 800
Tru1I TTAA 6 cut(s) 137, 234, 393, 749, 813, 933
Tru9I TTAA 6 cut(s) 137, 234, 393, 749, 813, 933
TscAI CASTG 3 cut(s) 256, 328, 672
TseFI GTSAC 1 cut(s) 443
TseI GCWGC 4 cut(s) 272, 698, 701, 922
Tsp45I GTSAC 1 cut(s) 443
TspDTI ATGAA 7 cut(s) 57, 204, 221, 507, 633, 702, 857
TspRI CASTG 3 cut(s) 256, 328, 672
Van91I CCANNNNNTGG 2 cut(s) 286, 1195
VpaK11BI GGWCC 1 cut(s) 72
XapI RAATTY 3 cut(s) 119, 610, 815
XmiI GTMKAC 2 cut(s) 1114, 1162
XmnI GAANNNNTTC 1 cut(s) 836
XspI CTAG 2 cut(s) 705, 996
ZrmI AGTACT 2 cut(s) 709, 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.