FvH4_2g35270

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005525
Physical Location & Seq
Reverse (-)
26034680 .. 26041666
6987 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35270.t3

Sequence Viewer

Length: 1419 bp
ATGGCGTTGAGTGAGGTTGAAGCTAAATTTCGACATGGCTTGTCTGAATCTGGAGAGGTGGATGATGATGGCAAACTCAAAAGAACTGGGACAGTATGGACTGCCTGTGCCCATATAATCACAGCATCAATAGGAGCTGGGGTGTTATCTCTAGCCTGGGCAATGGCCCAACTAGGATGGCTTGCAGGCATTTTCATTCTCTTGTTCTGCACCTTTGCTGCTGGTTACGCTTCCACTCTCTTAGCAGATTGTTACAGATTTCCTGACCCAGTTTCCGGCAAGAGAAACTACTCTTATATGGAGGCTGTCAAAGTCTATCTAGGTGGAACAATGCACAAGATTTGCGGATGGATGTTATATATAAACCTTGCTACCATTGGAGTTGGCTTCACTATAACCACGGCAAAAAGCTTGCTGGCTATACAGAAGTCTAATTGTCATCGCAAGTATGGTGAAGATTCAACATGCATGTTTTCCAGCATTCCACACATGGTTGGATTTGGGATTGTCGAAATTTTCTTATCACAACTCCAAAACTTTCACAAATTGACTTGGCTCTCTAAGCTTGCAGCCACTATGTCTTTTGTGTATGCAACTATTGGAATTGGACTTTCTCTTGCTAAGATCATATCAGGGCATGGAGGAACAACTTCTCTTGCAGGGGTAGAGCAGTCTTCATCGGAAAAAATTTGGATGACATTTATAGCAGCCGGAGATATTGCATTTGCTTGCTCGTATGCTCTTGTCCTGTTTGATATCCAAGACACACTGAAGTCATCGCCACCAGAGAACAAAGTGATGAAGAGGGCTGTTGTGATAGGGGGTTTGACTATGGCATTATTGTTCACGATGTGTGGCAGCTTCGGTTATGCTGCGTTCGGCAACAAGACACCCGAAAACCTGCTTGCTGGTTTTGGAGATGAGATGCCCTGGGCATTCTGGCTCCTAGATTTGGCTAATTTTTGTATTGTGGTGCACATAGTTGGTGCGTTTCAGGTACTGTGCCAACCAGTGTTTCGCATTGTCGAACTGTTGGCTAGAAGCAGGTGGCCAAAGTCCAATTTCTTTACCAAAGAGAACCCAGTCAAACTAGGAAACATGAAGTTCAATTTGAACATATTTAGGCTGACATGGAGAACATTCTATGTGGTGGTGGTGACTGTCATTGCCATGGCAATGCCATTCTTCACAAACATGCTTGCGCTTCTCGGAGCCATCGGGTACTGGCCTCTTATAGTTTATGTCCCAATAGAGCTGCACATTGCGCAAAACAAGATTCAGAAACCAACCCTCCGCTGGTTCGGGCTTCAACTTCTGAGCTTTTTGTGCTTGCTCATTTCCTTGGCTGCGGCATCTGGTTCCATTTACGGTCTGCGTAAGGGTCTCAGTGCCTACAAGCTCTTCCAAGTCAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

473

Amino Acids

51.94

Weight (kDa)

9.12

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 42 - 162 8.6e-30 Transmembrane amino acid transporter protein
Aa_trans PF01490 176 - 412 1.3e-50 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1035
Acc16I TGCGCA 1 cut(s) 1266
Acc36I ACCTGC 2 cut(s) 909, 1035
AciI CCGC 3 cut(s) 345, 1294, 1349
AcoI YGGCCR 1 cut(s) 1049
AcsI RAATTY 3 cut(s) 26, 513, 687
AcuI CTGAAG 1 cut(s) 791
AdeI CACNNNGTG 1 cut(s) 852
AfaI GTAC 2 cut(s) 999, 1223
AfiI CCNNNNNNNGG 3 cut(s) 275, 952, 1296
AgsI TTSAA 5 cut(s) 20, 462, 1108, 1114, 1310
AjnI CCWGG 2 cut(s) 155, 929
AjuI GAANNNNNNNTTGG 2 cut(s) 999, 1031
AluBI AGCT 8 cut(s) 23, 137, 411, 565, 861, 1255, 1320, 1399
AluI AGCT 8 cut(s) 23, 137, 411, 565, 861, 1255, 1320, 1399
Alw21I GWGCWC 1 cut(s) 978
Alw26I GTCTC 1 cut(s) 1388
Alw44I GTGCAC 1 cut(s) 974
AlwNI CAGNNNCTG 1 cut(s) 1000
AoxI GGCC 3 cut(s) 165, 1049, 1226
ApaLI GTGCAC 1 cut(s) 974
ApeKI GCWGC 7 cut(s) 218, 569, 707, 858, 872, 1255, 1346
ApoI RAATTY 3 cut(s) 26, 513, 687
Asp700I GAANNNNTTC 1 cut(s) 649
AspLEI GCGC 2 cut(s) 1204, 1267
AspS9I GGNCC 1 cut(s) 166
AsuHPI GGTGA 2 cut(s) 464, 1168
BaeGI GKGCMC 2 cut(s) 112, 978
BalI TGGCCA 1 cut(s) 1051
BbsI GAAGAC 1 cut(s) 666
Bbv12I GWGCWC 1 cut(s) 978
BbvI GCAGC 7 cut(s) 205, 581, 719, 859, 870, 1242, 1333
BccI CCATC 4 cut(s) 62, 171, 342, 1223
BceAI ACGGC 1 cut(s) 417
BciT130I CCWGG 2 cut(s) 157, 931
BcoDI GTCTC 1 cut(s) 1388
BfaI CTAG 6 cut(s) 152, 173, 320, 947, 1038, 1091
BfuAI ACCTGC 2 cut(s) 909, 1035
BisI GCNGC 8 cut(s) 219, 570, 708, 859, 873, 1256, 1347, 1350
BlsI GCNGC 8 cut(s) 220, 571, 709, 860, 874, 1257, 1348, 1351
Bme1390I CCNGG 2 cut(s) 157, 931
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 3 cut(s) 944, 1213, 1360
BmrFI CCNGG 2 cut(s) 157, 931
BmrI ACTGGG 3 cut(s) 96, 263, 1076
BmsI GCATC 3 cut(s) 134, 915, 1361
BmuI ACTGGG 3 cut(s) 96, 263, 1076
BpiI GAAGAC 1 cut(s) 666
BpmI CTGGAG 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 1388
BsaJI CCNNGG 6 cut(s) 156, 399, 929, 930, 1170, 1341
BsaXI ACNNNNNCTCC 4 cut(s) 126, 156, 1275, 1305
Bsc4I CCNNNNNNNGG 3 cut(s) 275, 952, 1296
Bse1I ACTGG 5 cut(s) 91, 269, 1010, 1082, 1229
Bse3DI GCAATG 4 cut(s) 168, 1164, 1182, 1260
BseBI CCWGG 2 cut(s) 157, 931
BseDI CCNNGG 6 cut(s) 156, 399, 929, 930, 1170, 1341
BseGI GGATG 5 cut(s) 67, 182, 353, 357, 699
BseLI CCNNNNNNNGG 3 cut(s) 275, 952, 1296
BseMI GCAATG 4 cut(s) 168, 1164, 1182, 1260
BseMII CTCAG 2 cut(s) 1307, 1399
BseNI ACTGG 5 cut(s) 91, 269, 1010, 1082, 1229
BseSI GKGCMC 2 cut(s) 112, 978
BseXI GCAGC 7 cut(s) 205, 581, 719, 859, 870, 1242, 1333
BseYI CCCAGC 1 cut(s) 137
BsgI GTGCAG 2 cut(s) 193, 1241
BshFI GGCC 3 cut(s) 167, 1051, 1228
BsiHKAI GWGCWC 1 cut(s) 978
BsiSI CCGG 2 cut(s) 276, 711
BslFI GGGAC 2 cut(s) 103, 1229
BslI CCNNNNNNNGG 3 cut(s) 275, 952, 1296
BsmAI GTCTC 1 cut(s) 1388
BsmFI GGGAC 2 cut(s) 103, 1229
BsmI GAATGC 2 cut(s) 480, 935
BsnI GGCC 3 cut(s) 167, 1051, 1228
Bso31I GGTCTC 1 cut(s) 1388
Bsp1286I GDGCHC 2 cut(s) 112, 978
Bsp143I GATC 1 cut(s) 624
Bsp19I CCATGG 1 cut(s) 1170
BspACI CCGC 3 cut(s) 345, 1294, 1349
BspANI GGCC 3 cut(s) 167, 1051, 1228
BspCNI CTCAG 2 cut(s) 1308, 1398
BspLI GGNNCC 3 cut(s) 944, 1213, 1360
BspMI ACCTGC 2 cut(s) 909, 1035
BspQI GCTCTTC 1 cut(s) 1406
BspTNI GGTCTC 1 cut(s) 1388
BsrDI GCAATG 4 cut(s) 168, 1164, 1182, 1260
BsrI ACTGG 5 cut(s) 91, 269, 1010, 1082, 1229
BssECI CCNNGG 6 cut(s) 156, 399, 929, 930, 1170, 1341
BssMI GATC 1 cut(s) 624
BssT1I CCWWGG 2 cut(s) 1170, 1341
Bst2UI CCWGG 2 cut(s) 157, 931
Bst4CI ACNGT 5 cut(s) 94, 1002, 1032, 1162, 1370
Bst6I CTCTTC 2 cut(s) 797, 1406
BstC8I GCNNGC 9 cut(s) 183, 187, 413, 417, 567, 730, 906, 1200, 1331
BstDEI CTNAG 5 cut(s) 241, 561, 621, 1316, 1385
BstDSI CCRYGG 2 cut(s) 399, 1170
BstF5I GGATG 5 cut(s) 67, 182, 353, 357, 699
BstHHI GCGC 2 cut(s) 1204, 1267
BstKTI GATC 1 cut(s) 627
BstMAI GTCTC 1 cut(s) 1388
BstMBI GATC 1 cut(s) 624
BstMWI GCNNNNNNNGC 4 cut(s) 227, 562, 1264, 1326
BstNI CCWGG 2 cut(s) 157, 931
BstNSI RCATGY 3 cut(s) 468, 472, 1198
BstSCI CCNGG 2 cut(s) 155, 929
BstSLI GKGCMC 2 cut(s) 112, 978
BstV1I GCAGC 7 cut(s) 205, 581, 719, 859, 870, 1242, 1333
BstV2I GAAGAC 1 cut(s) 666
BsuRI GGCC 3 cut(s) 167, 1051, 1228
BtgI CCRYGG 2 cut(s) 399, 1170
BtgZI GCGATG 2 cut(s) 425, 762
BtsCI GGATG 5 cut(s) 67, 182, 353, 357, 699
BtsIMutI CAGTG 3 cut(s) 767, 1017, 1393
BveI ACCTGC 2 cut(s) 909, 1035
Cac8I GCNNGC 9 cut(s) 183, 187, 413, 417, 567, 730, 906, 1200, 1331
CaiI CAGNNNCTG 1 cut(s) 1000
CfoI GCGC 2 cut(s) 1204, 1267
Cfr13I GGNCC 1 cut(s) 166
Csp6I GTAC 2 cut(s) 998, 1222
CviAII CATG 9 cut(s) 35, 465, 469, 490, 638, 1099, 1131, 1171, 1195
CviQI GTAC 2 cut(s) 998, 1222
DdeI CTNAG 5 cut(s) 241, 561, 621, 1316, 1385
DpnI GATC 1 cut(s) 626
DpnII GATC 1 cut(s) 624
DraIII CACNNNGTG 1 cut(s) 852
EaeI YGGCCR 1 cut(s) 1049
Eam1104I CTCTTC 2 cut(s) 797, 1406
EarI CTCTTC 2 cut(s) 797, 1406
Eco130I CCWWGG 2 cut(s) 1170, 1341
Eco31I GGTCTC 1 cut(s) 1388
Eco32I GATATC 1 cut(s) 757
Eco57I CTGAAG 1 cut(s) 791
EcoRII CCWGG 2 cut(s) 155, 929
EcoRV GATATC 1 cut(s) 757
EcoT14I CCWWGG 2 cut(s) 1170, 1341
EcoT22I ATGCAT 1 cut(s) 470
ErhI CCWWGG 2 cut(s) 1170, 1341
FaeI CATG 9 cut(s) 38, 468, 472, 493, 641, 1102, 1134, 1174, 1198
FaqI GGGAC 2 cut(s) 103, 1229
FatI CATG 9 cut(s) 34, 464, 468, 489, 637, 1098, 1130, 1170, 1194
Fnu4HI GCNGC 8 cut(s) 219, 570, 708, 859, 873, 1256, 1347, 1350
FokI GGATG 5 cut(s) 74, 189, 360, 364, 706
Fsp4HI GCNGC 8 cut(s) 219, 570, 708, 859, 873, 1256, 1347, 1350
FspBI CTAG 6 cut(s) 152, 173, 320, 947, 1038, 1091
FspI TGCGCA 1 cut(s) 1266
GlaI GCGC 2 cut(s) 1203, 1266
GluI GCNGC 8 cut(s) 219, 570, 708, 859, 873, 1256, 1347, 1350
GsaI CCCAGC 1 cut(s) 141
GsuI CTGGAG 1 cut(s) 72
HaeIII GGCC 3 cut(s) 167, 1051, 1228
HapII CCGG 2 cut(s) 276, 711
HhaI GCGC 2 cut(s) 1204, 1267
Hin1II CATG 9 cut(s) 38, 468, 472, 493, 641, 1102, 1134, 1174, 1198
Hin6I GCGC 2 cut(s) 1202, 1265
HinP1I GCGC 2 cut(s) 1202, 1265
HindIII AAGCTT 2 cut(s) 409, 563
HinfI GANTC 3 cut(s) 47, 458, 1276
HpaII CCGG 2 cut(s) 276, 711
HphI GGTGA 2 cut(s) 464, 1168
Hpy166II GTNNAC 2 cut(s) 846, 976
Hpy188I TCNGA 5 cut(s) 46, 682, 1211, 1281, 1317
Hpy188III TCNNGA 3 cut(s) 51, 263, 847
Hpy8I GTNNAC 2 cut(s) 846, 976
HpyCH4III ACNGT 5 cut(s) 94, 1002, 1032, 1162, 1370
HpyF10VI GCNNNNNNNGC 4 cut(s) 227, 562, 1264, 1326
HpyF3I CTNAG 5 cut(s) 241, 561, 621, 1316, 1385
Hsp92II CATG 9 cut(s) 38, 468, 472, 493, 641, 1102, 1134, 1174, 1198
HspAI GCGC 2 cut(s) 1202, 1265
Kzo9I GATC 1 cut(s) 624
LguI GCTCTTC 1 cut(s) 1406
LmnI GCTCC 3 cut(s) 134, 948, 1211
Lsp1109I GCAGC 7 cut(s) 205, 581, 719, 859, 870, 1242, 1333
LweI GCATC 3 cut(s) 134, 915, 1361
MaeI CTAG 6 cut(s) 152, 173, 320, 947, 1038, 1091
MaeIII GTNAC 3 cut(s) 224, 251, 1156
MalI GATC 1 cut(s) 626
MboI GATC 1 cut(s) 624
MboII GAAGA 5 cut(s) 467, 666, 814, 1177, 1393
MhlI GDGCHC 2 cut(s) 112, 978
MlsI TGGCCA 1 cut(s) 1051
MluCI AATT 9 cut(s) 26, 433, 513, 545, 603, 687, 958, 1060, 1108
MluNI TGGCCA 1 cut(s) 1051
MmeI TCCRAC 1 cut(s) 475
MnlI CCTC 7 cut(s) 7, 49, 295, 635, 798, 1239, 1301
Mox20I TGGCCA 1 cut(s) 1051
Mph1103I ATGCAT 1 cut(s) 470
MroXI GAANNNNTTC 1 cut(s) 649
MscI TGGCCA 1 cut(s) 1051
MslI CAYNNNNRTG 3 cut(s) 1169, 1175, 1193
Msp20I TGGCCA 1 cut(s) 1051
MspA1I CMGCKG 1 cut(s) 1296
MspI CCGG 2 cut(s) 276, 711
MspR9I CCNGG 2 cut(s) 157, 931
Mva1269I GAATGC 2 cut(s) 480, 935
MvaI CCWGG 2 cut(s) 157, 931
MwoI GCNNNNNNNGC 4 cut(s) 227, 562, 1264, 1326
NcoI CCATGG 1 cut(s) 1170
NdeII GATC 1 cut(s) 624
NlaIII CATG 9 cut(s) 38, 468, 472, 493, 641, 1102, 1134, 1174, 1198
NlaIV GGNNCC 3 cut(s) 944, 1213, 1360
NmuCI GTSAC 1 cut(s) 1156
NsbI TGCGCA 1 cut(s) 1266
NsiI ATGCAT 1 cut(s) 470
NspI RCATGY 3 cut(s) 468, 472, 1198
PaqCI CACCTGC 1 cut(s) 1035
PasI CCCWGGG 1 cut(s) 930
PciSI GCTCTTC 1 cut(s) 1406
PctI GAATGC 2 cut(s) 480, 935
PdmI GAANNNNTTC 1 cut(s) 649
PfeI GAWTC 3 cut(s) 47, 458, 1276
PkrI GCNGC 8 cut(s) 220, 571, 709, 860, 874, 1257, 1348, 1351
Psp6I CCWGG 2 cut(s) 155, 929
PspFI CCCAGC 1 cut(s) 137
PspGI CCWGG 2 cut(s) 155, 929
PspN4I GGNNCC 3 cut(s) 944, 1213, 1360
PspPI GGNCC 1 cut(s) 166
PstNI CAGNNNCTG 1 cut(s) 1000
RsaI GTAC 2 cut(s) 999, 1223
RsaNI GTAC 2 cut(s) 998, 1222
RseI CAYNNNNRTG 3 cut(s) 1169, 1175, 1193
SapI GCTCTTC 1 cut(s) 1406
SatI GCNGC 8 cut(s) 219, 570, 708, 859, 873, 1256, 1347, 1350
Sau3AI GATC 1 cut(s) 624
Sau96I GGNCC 1 cut(s) 166
ScrFI CCNGG 2 cut(s) 157, 931
SduI GDGCHC 2 cut(s) 112, 978
SfaNI GCATC 3 cut(s) 134, 915, 1361
SmiMI CAYNNNNRTG 3 cut(s) 1169, 1175, 1193
Sse9I AATT 9 cut(s) 26, 433, 513, 545, 603, 687, 958, 1060, 1108
SsiI CCGC 3 cut(s) 345, 1294, 1349
SspMI CTAG 6 cut(s) 152, 173, 320, 947, 1038, 1091
StyD4I CCNGG 2 cut(s) 155, 929
StyI CCWWGG 2 cut(s) 1170, 1341
TaaI ACNGT 5 cut(s) 94, 1002, 1032, 1162, 1370
TaqI TCGA 3 cut(s) 31, 510, 1026
TasI AATT 9 cut(s) 26, 433, 513, 545, 603, 687, 958, 1060, 1108
TauI GCSGC 1 cut(s) 1352
TfiI GAWTC 3 cut(s) 47, 458, 1276
TscAI CASTG 3 cut(s) 774, 1017, 1393
TseFI GTSAC 1 cut(s) 1156
TseI GCWGC 7 cut(s) 218, 569, 707, 858, 872, 1255, 1346
Tsp45I GTSAC 1 cut(s) 1156
TspDTI ATGAA 4 cut(s) 184, 666, 815, 1115
TspRI CASTG 3 cut(s) 774, 1017, 1393
VneI GTGCAC 1 cut(s) 974
XapI RAATTY 3 cut(s) 26, 513, 687
XceI RCATGY 3 cut(s) 468, 472, 1198
XcmI CCANNNNNNNNNTGG 1 cut(s) 1293
XmnI GAANNNNTTC 1 cut(s) 649
XspI CTAG 6 cut(s) 152, 173, 320, 947, 1038, 1091
Zsp2I ATGCAT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.