RchiOBHm_Chr6g0306671

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005525
Physical Location & Seq
Forward (+)
65292384 .. 65293973
1590 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27569

Sequence Viewer

Length: 810 bp
ATGACATCCCCATGGCCGGCTGCGGCTGTGTTCCAAGAATGGGTCCGATTAGTTCTATCGCGGTGGTCGACGCTTCAATTGGTCATCGAGAACGAGTGGGGTAGCCGTGATTCGCGCCTCAAGACCGAGCAACTCGTCTTCGATGTCCTCTCCTGGGTCAATCACTCCACCGTGGGAGATATTGCATTTGCTTGCTCGTATGCTCTTGTCCTGTTTGAGATTCAAGACACACTGAAGTCATCGCCACCAGAAAACAAAGTGATGAAGAGGGCTGTTGTGATAGGGGGTTCTACTATGACATTATTCTTCATGATGTATGCTAGCTTGGGTTATGCTGCCTTCGGCAACAAGACACCAGAAAACCTGCTTGCTGTTGGTGCATTTCAGGTACTATGCCAACCAGTGTTTGGCATTGTTGAACTGTTGGCTAGTAGAAGGTGGCCAAAGTCCAATTTCATTAACAAAGAGAACCAAGTCAGACTAGGGTACATGAAGTTCGATATAAACATGTTTAGGCTGACATGGAGAACAACCTTTGTGGTGTTGGCGACTTTCATTGACATGGCAATGCCATTCTTCACAGACATGCTTGCGCTTCTTGGAGCTATCGGGTACTGGCCTCTTATAGTTTATGTCCCAATAGAGATGCATGTTGTGCAAAACAAGATTCAGAAACAAACCCTTCGCTGGTTCGGGCTCCAACTCCTGAGCTTTTTGTGCTTGCTTCTTTCCTTGGCCGCAGCATCTGGCTCCACTTATGGTCTGCATAAGGGTCTCGGTGCGTACAAGCTCTTCAAAGTTAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

269

Amino Acids

30.52

Weight (kDa)

9.34

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WGG PF10273 11 - 56 3.1e-09 Pre-rRNA-processing protein TSR2
Aa_trans PF01490 50 - 123 4.5e-16 Transmembrane amino acid transporter protein
Aa_trans PF01490 124 - 255 6.1e-19 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 372
AccB7I CCANNNNNTGG 1 cut(s) 407
AccI GTMKAC 1 cut(s) 68
AccII CGCG 2 cut(s) 61, 115
AciI CCGC 3 cut(s) 23, 61, 738
AcoI YGGCCR 3 cut(s) 14, 440, 735
AcuI CTGAAG 1 cut(s) 254
AfaI GTAC 4 cut(s) 390, 488, 614, 785
AfiI CCNNNNNNNGG 5 cut(s) 16, 40, 154, 407, 687
AflIII ACRYGT 1 cut(s) 507
AgsI TTSAA 4 cut(s) 77, 224, 419, 796
AjnI CCWGG 1 cut(s) 152
AluBI AGCT 4 cut(s) 324, 605, 711, 790
AluI AGCT 4 cut(s) 324, 605, 711, 790
Alw26I GTCTC 1 cut(s) 779
AlwNI CAGNNNCTG 1 cut(s) 746
AoxI GGCC 4 cut(s) 14, 440, 617, 735
ApeKI GCWGC 3 cut(s) 20, 335, 740
AspLEI GCGC 2 cut(s) 117, 595
AspS9I GGNCC 1 cut(s) 43
AsuNHI GCTAGC 1 cut(s) 320
AvaII GGWCC 1 cut(s) 43
BalI TGGCCA 1 cut(s) 442
BanII GRGCYC 1 cut(s) 699
BarI GAAGNNNNNNTAC 2 cut(s) 776, 808
BbsI GAAGAC 1 cut(s) 130
BbvI GCAGC 3 cut(s) 7, 322, 752
BceAI ACGGC 1 cut(s) 90
BciT130I CCWGG 1 cut(s) 154
BcoDI GTCTC 1 cut(s) 779
BfaI CTAG 3 cut(s) 321, 429, 482
BfuAI ACCTGC 1 cut(s) 372
BisI GCNGC 5 cut(s) 21, 24, 336, 738, 741
BlsI GCNGC 5 cut(s) 22, 25, 337, 739, 742
Bme1390I CCNGG 1 cut(s) 154
Bme18I GGWCC 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 43
BmiI GGNNCC 3 cut(s) 44, 698, 751
BmrFI CCNGG 1 cut(s) 154
BmsI GCATC 2 cut(s) 636, 752
BmtI GCTAGC 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 130
Bpu10I CCTNAGC 1 cut(s) 707
BpuEI CTTGAG 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 779
BsaJI CCNNGG 4 cut(s) 11, 153, 171, 732
Bsc4I CCNNNNNNNGG 5 cut(s) 16, 40, 154, 407, 687
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 2 cut(s) 401, 620
Bse3DI GCAATG 1 cut(s) 573
BseBI CCWGG 1 cut(s) 154
BseDI CCNNGG 4 cut(s) 11, 153, 171, 732
BseGI GGATG 1 cut(s) 5
BseLI CCNNNNNNNGG 5 cut(s) 16, 40, 154, 407, 687
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 1 cut(s) 698
BseNI ACTGG 2 cut(s) 401, 620
BseXI GCAGC 3 cut(s) 7, 322, 752
Bsh1236I CGCG 2 cut(s) 61, 115
BshFI GGCC 4 cut(s) 16, 442, 619, 737
BsiSI CCGG 1 cut(s) 17
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 5 cut(s) 16, 40, 154, 407, 687
BsmAI GTCTC 1 cut(s) 779
BsmFI GGGAC 1 cut(s) 620
BsnI GGCC 4 cut(s) 16, 442, 619, 737
Bso31I GGTCTC 1 cut(s) 779
Bsp1286I GDGCHC 1 cut(s) 699
Bsp19I CCATGG 1 cut(s) 11
BspACI CCGC 3 cut(s) 23, 61, 738
BspANI GGCC 4 cut(s) 16, 442, 619, 737
BspCNI CTCAG 1 cut(s) 699
BspFNI CGCG 2 cut(s) 61, 115
BspHI TCATGA 1 cut(s) 309
BspLI GGNNCC 3 cut(s) 44, 698, 751
BspMI ACCTGC 1 cut(s) 372
BspOI GCTAGC 1 cut(s) 324
BspQI GCTCTTC 1 cut(s) 797
BspTNI GGTCTC 1 cut(s) 779
BsrDI GCAATG 1 cut(s) 573
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 2 cut(s) 401, 620
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 4 cut(s) 11, 153, 171, 732
BssT1I CCWWGG 2 cut(s) 11, 732
Bst2UI CCWGG 1 cut(s) 154
Bst4CI ACNGT 2 cut(s) 172, 423
Bst6I CTCTTC 2 cut(s) 260, 797
BstAPI GCANNNNNTGC 1 cut(s) 655
BstC8I GCNNGC 6 cut(s) 18, 193, 322, 369, 591, 722
BstDEI CTNAG 1 cut(s) 707
BstDSI CCRYGG 2 cut(s) 11, 171
BstF5I GGATG 1 cut(s) 5
BstFNI CGCG 2 cut(s) 61, 115
BstHHI GCGC 2 cut(s) 117, 595
BstMAI GTCTC 1 cut(s) 779
BstMWI GCNNNNNNNGC 3 cut(s) 377, 655, 717
BstNI CCWGG 1 cut(s) 154
BstNSI RCATGY 3 cut(s) 511, 589, 653
BstSCI CCNGG 1 cut(s) 152
BstUI CGCG 2 cut(s) 61, 115
BstV1I GCAGC 3 cut(s) 7, 322, 752
BstV2I GAAGAC 1 cut(s) 130
BsuRI GGCC 4 cut(s) 16, 442, 619, 737
BtgI CCRYGG 2 cut(s) 11, 171
BtgZI GCGATG 1 cut(s) 225
BtsCI GGATG 1 cut(s) 5
BtsIMutI CAGTG 2 cut(s) 230, 408
BveI ACCTGC 1 cut(s) 372
Cac8I GCNNGC 6 cut(s) 18, 193, 322, 369, 591, 722
CaiI CAGNNNCTG 1 cut(s) 746
CciI TCATGA 1 cut(s) 309
CfoI GCGC 2 cut(s) 117, 595
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 43
CseI GACGC 1 cut(s) 79
Csp6I GTAC 4 cut(s) 389, 487, 613, 784
CspCI CAANNNNNGTGG 2 cut(s) 519, 554
CviAII CATG 8 cut(s) 12, 310, 490, 508, 522, 562, 586, 650
CviQI GTAC 4 cut(s) 389, 487, 613, 784
DdeI CTNAG 1 cut(s) 707
EaeI YGGCCR 3 cut(s) 14, 440, 735
Eam1104I CTCTTC 2 cut(s) 260, 797
EarI CTCTTC 2 cut(s) 260, 797
Eco130I CCWWGG 2 cut(s) 11, 732
Eco24I GRGCYC 1 cut(s) 699
Eco31I GGTCTC 1 cut(s) 779
Eco47I GGWCC 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 152
EcoT14I CCWWGG 2 cut(s) 11, 732
EcoT22I ATGCAT 1 cut(s) 651
EcoT38I GRGCYC 1 cut(s) 699
ErhI CCWWGG 2 cut(s) 11, 732
FaeI CATG 8 cut(s) 15, 313, 493, 511, 525, 565, 589, 653
FaqI GGGAC 1 cut(s) 620
FatI CATG 8 cut(s) 11, 309, 489, 507, 521, 561, 585, 649
FblI GTMKAC 1 cut(s) 68
Fnu4HI GCNGC 5 cut(s) 21, 24, 336, 738, 741
FriOI GRGCYC 1 cut(s) 699
Fsp4HI GCNGC 5 cut(s) 21, 24, 336, 738, 741
FspBI CTAG 3 cut(s) 321, 429, 482
GlaI GCGC 2 cut(s) 116, 594
GluI GCNGC 5 cut(s) 21, 24, 336, 738, 741
HaeIII GGCC 4 cut(s) 16, 442, 619, 737
HapII CCGG 1 cut(s) 17
HgaI GACGC 1 cut(s) 79
HhaI GCGC 2 cut(s) 117, 595
Hin1II CATG 8 cut(s) 15, 313, 493, 511, 525, 565, 589, 653
Hin6I GCGC 2 cut(s) 115, 593
HinP1I GCGC 2 cut(s) 115, 593
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HinfI GANTC 3 cut(s) 110, 220, 667
HpaII CCGG 1 cut(s) 17
Hpy166II GTNNAC 1 cut(s) 69
Hpy188I TCNGA 3 cut(s) 47, 479, 672
Hpy188III TCNNGA 5 cut(s) 88, 121, 224, 310, 706
Hpy8I GTNNAC 1 cut(s) 69
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 3 cut(s) 349, 429, 692
HpyCH4III ACNGT 2 cut(s) 172, 423
HpyCH4V TGCA 5 cut(s) 185, 380, 649, 658, 766
HpyF10VI GCNNNNNNNGC 3 cut(s) 377, 655, 717
HpyF3I CTNAG 1 cut(s) 707
Hsp92II CATG 8 cut(s) 15, 313, 493, 511, 525, 565, 589, 653
HspAI GCGC 2 cut(s) 115, 593
KroI GCCGGC 1 cut(s) 16
KroNI GCCGGC 1 cut(s) 18
LguI GCTCTTC 1 cut(s) 797
LmnI GCTCC 3 cut(s) 602, 702, 755
Lsp1109I GCAGC 3 cut(s) 7, 322, 752
LweI GCATC 2 cut(s) 636, 752
MaeI CTAG 3 cut(s) 321, 429, 482
MboII GAAGA 5 cut(s) 130, 277, 298, 568, 784
MfeI CAATTG 1 cut(s) 77
MhlI GDGCHC 1 cut(s) 699
MlsI TGGCCA 1 cut(s) 442
MluCI AATT 2 cut(s) 77, 451
MluNI TGGCCA 1 cut(s) 442
MmeI TCCRAC 1 cut(s) 724
MnlI CCTC 4 cut(s) 128, 158, 261, 630
Mox20I TGGCCA 1 cut(s) 442
Mph1103I ATGCAT 1 cut(s) 651
MroNI GCCGGC 1 cut(s) 16
MscI TGGCCA 1 cut(s) 442
MseI TTAA 2 cut(s) 459, 801
MslI CAYNNNNRTG 4 cut(s) 10, 560, 566, 584
Msp20I TGGCCA 1 cut(s) 442
MspI CCGG 1 cut(s) 17
MspR9I CCNGG 1 cut(s) 154
MunI CAATTG 1 cut(s) 77
MvaI CCWGG 1 cut(s) 154
MvnI CGCG 2 cut(s) 61, 115
MwoI GCNNNNNNNGC 3 cut(s) 377, 655, 717
NaeI GCCGGC 1 cut(s) 18
NcoI CCATGG 1 cut(s) 11
NgoMIV GCCGGC 1 cut(s) 16
NheI GCTAGC 1 cut(s) 320
NlaIII CATG 8 cut(s) 15, 313, 493, 511, 525, 565, 589, 653
NlaIV GGNNCC 3 cut(s) 44, 698, 751
NsiI ATGCAT 1 cut(s) 651
NspI RCATGY 3 cut(s) 511, 589, 653
PagI TCATGA 1 cut(s) 309
PciI ACATGT 1 cut(s) 507
PciSI GCTCTTC 1 cut(s) 797
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PdiI GCCGGC 1 cut(s) 18
PfeI GAWTC 3 cut(s) 110, 220, 667
PflMI CCANNNNNTGG 1 cut(s) 407
PkrI GCNGC 5 cut(s) 22, 25, 337, 739, 742
PscI ACATGT 1 cut(s) 507
Psp6I CCWGG 1 cut(s) 152
PspGI CCWGG 1 cut(s) 152
PspN4I GGNNCC 3 cut(s) 44, 698, 751
PspPI GGNCC 1 cut(s) 43
PsrI GAACNNNNNNTAC 2 cut(s) 479, 511
PstNI CAGNNNCTG 1 cut(s) 746
RsaI GTAC 4 cut(s) 390, 488, 614, 785
RsaNI GTAC 4 cut(s) 389, 487, 613, 784
RseI CAYNNNNRTG 4 cut(s) 10, 560, 566, 584
SalI GTCGAC 1 cut(s) 67
SapI GCTCTTC 1 cut(s) 797
SaqAI TTAA 2 cut(s) 459, 801
SatI GCNGC 5 cut(s) 21, 24, 336, 738, 741
Sau96I GGNCC 1 cut(s) 43
ScrFI CCNGG 1 cut(s) 154
SduI GDGCHC 1 cut(s) 699
SetI ASST 8 cut(s) 326, 366, 390, 440, 536, 607, 713, 792
SfaNI GCATC 2 cut(s) 636, 752
SinI GGWCC 1 cut(s) 43
SmiMI CAYNNNNRTG 4 cut(s) 10, 560, 566, 584
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 2 cut(s) 77, 451
SsiI CCGC 3 cut(s) 23, 61, 738
SspMI CTAG 3 cut(s) 321, 429, 482
StyD4I CCNGG 1 cut(s) 152
StyI CCWWGG 2 cut(s) 11, 732
TaaI ACNGT 2 cut(s) 172, 423
TaqI TCGA 4 cut(s) 68, 87, 141, 498
TaqII GACCGA 1 cut(s) 140
TasI AATT 2 cut(s) 77, 451
TauI GCSGC 2 cut(s) 26, 740
TfiI GAWTC 3 cut(s) 110, 220, 667
Tru1I TTAA 2 cut(s) 459, 801
Tru9I TTAA 2 cut(s) 459, 801
TscAI CASTG 2 cut(s) 237, 408
TseI GCWGC 3 cut(s) 20, 335, 740
TspDTI ATGAA 5 cut(s) 278, 298, 445, 506, 544
TspRI CASTG 2 cut(s) 237, 408
Van91I CCANNNNNTGG 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 43
XceI RCATGY 3 cut(s) 511, 589, 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 68
XspI CTAG 3 cut(s) 321, 429, 482
Zsp2I ATGCAT 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.