Rh6AG463800

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0005525
Physical Location & Seq
Forward (+)
64654905 .. 64655933
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG463800.1

Sequence Viewer

Length: 654 bp
ATGATGCCTATAGCAGTGGGAGATATTGCATTTGCTTGCTCGTATGCTCTTGTCCTGTTTGAGATTCAAGACACACTGAAGTCATCGCCACCAGAAAACAAAGTGATGAAGAGGGCTGTTGTGATAGGGGGTTCTACTATGACATTATTCTTCATGATGTATGCTAGCTTGGGTTATGCTGCCTTCGGCAACAAGACACCAGAAAACCTGCTTGCTGTTGGTGCATTTCAGGTACTATGCCAACCAGTGTTTGGCATTGTTGAACTGTTGGCTAGTAGAAGGTGGCCAAAGTCCAATTTCATTAACAAAGAGAACCAAGTCAGACTAGGGTACATGAAGTTCGATATAAACATGTTTAGGCTGACATGGAGAACAACCTTTGTGGTGTTGGCGACTTTCATTGACATGGCAATGCCATTCTTCACAGACATGCTTGCGCTTCTTGGAGCTATCGGGTACTGGCCTCTTATAGTTTATGTCCCAATAGAGATGCATGTTGTGCAAAACAAGATTCAGAAACAAACCCTTCGCTGGTTCGGGCTCCAACTCCTGAGCTTTTTGTGCTTGCTTCTTTCCTTGGCCGCAGCATCTGGCTCCACTTATGGTCTGCATAAGGGTCTCGGTGCGTACAAGCTCTTCAAAGTTAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

217

Amino Acids

24.38

Weight (kDa)

9.47

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 3 - 71 3.6e-16 Transmembrane amino acid transporter protein
Aa_trans PF01490 72 - 203 3.7e-19 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 216
AccB7I CCANNNNNTGG 1 cut(s) 251
AciI CCGC 1 cut(s) 582
AcoI YGGCCR 2 cut(s) 284, 579
AcuI CTGAAG 1 cut(s) 98
AfaI GTAC 4 cut(s) 234, 332, 458, 629
AfiI CCNNNNNNNGG 2 cut(s) 251, 531
AflIII ACRYGT 1 cut(s) 351
AgsI TTSAA 3 cut(s) 68, 263, 640
AluBI AGCT 4 cut(s) 168, 449, 555, 634
AluI AGCT 4 cut(s) 168, 449, 555, 634
Alw26I GTCTC 1 cut(s) 623
AlwNI CAGNNNCTG 1 cut(s) 590
AoxI GGCC 3 cut(s) 284, 461, 579
ApeKI GCWGC 2 cut(s) 179, 584
AspLEI GCGC 1 cut(s) 439
AsuNHI GCTAGC 1 cut(s) 164
BalI TGGCCA 1 cut(s) 286
BanII GRGCYC 1 cut(s) 543
BarI GAAGNNNNNNTAC 2 cut(s) 620, 652
BbvI GCAGC 2 cut(s) 166, 596
BcoDI GTCTC 1 cut(s) 623
BfaI CTAG 3 cut(s) 165, 273, 326
BfmI CTRYAG 1 cut(s) 9
BfuAI ACCTGC 1 cut(s) 216
BisI GCNGC 3 cut(s) 180, 582, 585
BlsI GCNGC 3 cut(s) 181, 583, 586
BmiI GGNNCC 2 cut(s) 542, 595
BmsI GCATC 2 cut(s) 480, 596
BmtI GCTAGC 1 cut(s) 168
Bpu10I CCTNAGC 1 cut(s) 551
BsaI GGTCTC 1 cut(s) 623
BsaJI CCNNGG 1 cut(s) 576
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 531
Bse1I ACTGG 2 cut(s) 245, 464
Bse3DI GCAATG 1 cut(s) 417
BseDI CCNNGG 1 cut(s) 576
BseLI CCNNNNNNNGG 2 cut(s) 251, 531
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 1 cut(s) 542
BseNI ACTGG 2 cut(s) 245, 464
BseXI GCAGC 2 cut(s) 166, 596
BshFI GGCC 3 cut(s) 286, 463, 581
BslFI GGGAC 1 cut(s) 464
BslI CCNNNNNNNGG 2 cut(s) 251, 531
BsmAI GTCTC 1 cut(s) 623
BsmFI GGGAC 1 cut(s) 464
BsnI GGCC 3 cut(s) 286, 463, 581
Bso31I GGTCTC 1 cut(s) 623
Bsp1286I GDGCHC 1 cut(s) 543
BspACI CCGC 1 cut(s) 582
BspANI GGCC 3 cut(s) 286, 463, 581
BspCNI CTCAG 1 cut(s) 543
BspHI TCATGA 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 542, 595
BspMI ACCTGC 1 cut(s) 216
BspOI GCTAGC 1 cut(s) 168
BspQI GCTCTTC 1 cut(s) 641
BspTNI GGTCTC 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 417
BsrI ACTGG 2 cut(s) 245, 464
BssECI CCNNGG 1 cut(s) 576
BssT1I CCWWGG 1 cut(s) 576
Bst4CI ACNGT 1 cut(s) 267
Bst6I CTCTTC 2 cut(s) 104, 641
BstAPI GCANNNNNTGC 1 cut(s) 499
BstC8I GCNNGC 5 cut(s) 37, 166, 213, 435, 566
BstDEI CTNAG 1 cut(s) 551
BstHHI GCGC 1 cut(s) 439
BstMAI GTCTC 1 cut(s) 623
BstMWI GCNNNNNNNGC 3 cut(s) 221, 499, 561
BstNSI RCATGY 3 cut(s) 355, 433, 497
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 2 cut(s) 166, 596
BsuRI GGCC 3 cut(s) 286, 463, 581
BtgZI GCGATG 1 cut(s) 69
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 3 cut(s) 21, 74, 252
BveI ACCTGC 1 cut(s) 216
Cac8I GCNNGC 5 cut(s) 37, 166, 213, 435, 566
CaiI CAGNNNCTG 1 cut(s) 590
CciI TCATGA 1 cut(s) 153
CfoI GCGC 1 cut(s) 439
Csp6I GTAC 4 cut(s) 233, 331, 457, 628
CspCI CAANNNNNGTGG 2 cut(s) 363, 398
CviAII CATG 7 cut(s) 154, 334, 352, 366, 406, 430, 494
CviQI GTAC 4 cut(s) 233, 331, 457, 628
DdeI CTNAG 1 cut(s) 551
EaeI YGGCCR 2 cut(s) 284, 579
Eam1104I CTCTTC 2 cut(s) 104, 641
EarI CTCTTC 2 cut(s) 104, 641
Eco130I CCWWGG 1 cut(s) 576
Eco24I GRGCYC 1 cut(s) 543
Eco31I GGTCTC 1 cut(s) 623
Eco57I CTGAAG 1 cut(s) 98
EcoT14I CCWWGG 1 cut(s) 576
EcoT22I ATGCAT 1 cut(s) 495
EcoT38I GRGCYC 1 cut(s) 543
ErhI CCWWGG 1 cut(s) 576
FaeI CATG 7 cut(s) 157, 337, 355, 369, 409, 433, 497
FaqI GGGAC 1 cut(s) 464
FatI CATG 7 cut(s) 153, 333, 351, 365, 405, 429, 493
Fnu4HI GCNGC 3 cut(s) 180, 582, 585
FriOI GRGCYC 1 cut(s) 543
Fsp4HI GCNGC 3 cut(s) 180, 582, 585
FspBI CTAG 3 cut(s) 165, 273, 326
GlaI GCGC 1 cut(s) 438
GluI GCNGC 3 cut(s) 180, 582, 585
HaeIII GGCC 3 cut(s) 286, 463, 581
HhaI GCGC 1 cut(s) 439
Hin1II CATG 7 cut(s) 157, 337, 355, 369, 409, 433, 497
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HinfI GANTC 2 cut(s) 64, 511
Hpy188I TCNGA 2 cut(s) 323, 516
Hpy188III TCNNGA 3 cut(s) 68, 154, 550
HpyAV CCTTC 3 cut(s) 193, 273, 536
HpyCH4III ACNGT 1 cut(s) 267
HpyCH4V TGCA 5 cut(s) 29, 224, 493, 502, 610
HpyF10VI GCNNNNNNNGC 3 cut(s) 221, 499, 561
HpyF3I CTNAG 1 cut(s) 551
Hsp92II CATG 7 cut(s) 157, 337, 355, 369, 409, 433, 497
HspAI GCGC 1 cut(s) 437
LguI GCTCTTC 1 cut(s) 641
LmnI GCTCC 3 cut(s) 446, 546, 599
Lsp1109I GCAGC 2 cut(s) 166, 596
LweI GCATC 2 cut(s) 480, 596
MaeI CTAG 3 cut(s) 165, 273, 326
MboII GAAGA 4 cut(s) 121, 142, 412, 628
MhlI GDGCHC 1 cut(s) 543
MlsI TGGCCA 1 cut(s) 286
MluCI AATT 1 cut(s) 295
MluNI TGGCCA 1 cut(s) 286
MmeI TCCRAC 1 cut(s) 568
MnlI CCTC 2 cut(s) 105, 474
Mox20I TGGCCA 1 cut(s) 286
Mph1103I ATGCAT 1 cut(s) 495
MscI TGGCCA 1 cut(s) 286
MseI TTAA 2 cut(s) 303, 645
MslI CAYNNNNRTG 3 cut(s) 404, 410, 428
Msp20I TGGCCA 1 cut(s) 286
MwoI GCNNNNNNNGC 3 cut(s) 221, 499, 561
NheI GCTAGC 1 cut(s) 164
NlaIII CATG 7 cut(s) 157, 337, 355, 369, 409, 433, 497
NlaIV GGNNCC 2 cut(s) 542, 595
NsiI ATGCAT 1 cut(s) 495
NspI RCATGY 3 cut(s) 355, 433, 497
PagI TCATGA 1 cut(s) 153
PciI ACATGT 1 cut(s) 351
PciSI GCTCTTC 1 cut(s) 641
PfeI GAWTC 2 cut(s) 64, 511
PflMI CCANNNNNTGG 1 cut(s) 251
PkrI GCNGC 3 cut(s) 181, 583, 586
PscI ACATGT 1 cut(s) 351
PspN4I GGNNCC 2 cut(s) 542, 595
PsrI GAACNNNNNNTAC 2 cut(s) 323, 355
PstNI CAGNNNCTG 1 cut(s) 590
RsaI GTAC 4 cut(s) 234, 332, 458, 629
RsaNI GTAC 4 cut(s) 233, 331, 457, 628
RseI CAYNNNNRTG 3 cut(s) 404, 410, 428
SapI GCTCTTC 1 cut(s) 641
SaqAI TTAA 2 cut(s) 303, 645
SatI GCNGC 3 cut(s) 180, 582, 585
SduI GDGCHC 1 cut(s) 543
SetI ASST 8 cut(s) 170, 210, 234, 284, 380, 451, 557, 636
SfaNI GCATC 2 cut(s) 480, 596
SfcI CTRYAG 1 cut(s) 9
SmiMI CAYNNNNRTG 3 cut(s) 404, 410, 428
Sse9I AATT 1 cut(s) 295
SsiI CCGC 1 cut(s) 582
SspMI CTAG 3 cut(s) 165, 273, 326
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 342
TasI AATT 1 cut(s) 295
TauI GCSGC 1 cut(s) 584
TfiI GAWTC 2 cut(s) 64, 511
Tru1I TTAA 2 cut(s) 303, 645
Tru9I TTAA 2 cut(s) 303, 645
TscAI CASTG 3 cut(s) 21, 81, 252
TseI GCWGC 2 cut(s) 179, 584
TspDTI ATGAA 5 cut(s) 122, 142, 289, 350, 388
TspRI CASTG 3 cut(s) 21, 81, 252
Van91I CCANNNNNTGG 1 cut(s) 251
XceI RCATGY 3 cut(s) 355, 433, 497
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XspI CTAG 3 cut(s) 165, 273, 326
Zsp2I ATGCAT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.