Rroxscaffold_7G00161580

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005525
Physical Location & Seq
Reverse (-)
4300837 .. 4304137
3301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00161580.1

Sequence Viewer

Length: 1461 bp
ATGGCGTTGAGTGAGGTTGAAGCTGAATTTCAACATGACTCATCTGAATCTGATTTGAACAATCCGGCGAAGTACGGGGAAGCTGGAGAAGAACTACTGGTGGATGATGATGGCAAACTCAAAAGGACTGGAACAGTATGGACTGCCTGTGCCCATATAATCACAGCATCAATAGGAGCTGGAGTGCTATCTTTAGCCTGGGCAATGGCCCAACTCGGCTGGCTTGCCGGCATTTTCATTCTCTTGTTCTGCACCTTCGCTGCTGGCTACGCTGCCACTCTCTTAGCAGATTGTTACAGATTTCCGGACCCAGTGTCAGGCAAGAGAAACTACTCCTATATGGAGGCTGTCAAAGTCTATTTAGGTGGAACAATGCACAAGATTTGTGGATGGATGTTATATTTAAACCTTGCTACCATTGGAGTTGGCTTCACTATAACCGCCGCAAAAAGCTTGGTGGCTATACAGAAGTCGAGTTGTCATCGCAAAAATGGTGAAGATTCTCCATGCATGTTTTCCAACATTCCACATATGGTTGGATTTGGGCTTGTTGAAATTTTCTTATCACAACTCCAAAACTTTCACAAGTTGACTCGGCTCTCTAAGCTTGCAGCCATGATGTCTTTTGTCTATGCAACTATTGGAATTGGACTTTCTCTAGCTAAGATCATATCAGGGGATGGAGGAGCAACATATCTTGCAGGGGCGGACCAGTCGTCATCGGAAAAAATTTGGATGATGTTTATAGCAGCCGGAGATATTGCATTTGCTTGCTCGTATTCTCTTATCCTGTTTGATATCCAAGACACACTGAAGTCATCGCCACCAGAGAACAAAGTGATGAAGAGGGCTGTCGTTATAGGGGGTTTGACTATGGCATTACTCTTCATGATGTGTGGTAGCTTGGGTTATGCCGCGTTTGGCAACTTCACACCCGAAAACCTGCTTGCTGGTTTTGGAGATGAGATGCCCTGGGCATTCTGGCTCCTGGATTTGGCTAATTTTTGCATTGTGGTGCACATAATTGGTGCATTTCAGGTACTGTGCCAACCAGTGTTTCGCATTGTCGAACTGTTGGCTAGAAGAAGGTGGCCAAAATCCAATTTCATTACCAAAGAGAACCCGGTCATACTAGGGAAGATGAAGTTCAATATAAACATGTTTAGGCTGACATGGAGAACATCCTTTGTGGTGTTGGTAACTGTCATTGCCATGGCAATGCCATTCTTCACAGATATGCTGGCGCTTCTCGGAGCTATCGGGTACTGGCCTCTTATAGTTTATGTCCCAATAAAGATGCACATTGCGCAAAACAAGATTCAGAAACAAACCCTTCGCTGGTTCGGGCTCCAACTCCTGAGCTTTTTGTGCTTGCTTCTTTCCTTGGCCGCGACATCTGGTTCCATTTATGGTCTGCATAAGGGTCTCGGTGCGTACAAGCTCTTCCAAGTTAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

486

Amino Acids

53.45

Weight (kDa)

8.64

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 42 - 472 3e-99 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1308
Acc36I ACCTGC 1 cut(s) 951
AccII CGCG 2 cut(s) 917, 1391
AccIII TCCGGA 1 cut(s) 304
AciI CCGC 5 cut(s) 441, 444, 707, 915, 1389
AcoI YGGCCR 2 cut(s) 1091, 1386
AcsI RAATTY 3 cut(s) 26, 555, 729
AcuI CTGAAG 1 cut(s) 833
AfaI GTAC 4 cut(s) 74, 1041, 1265, 1436
AfiI CCNNNNNNNGG 3 cut(s) 317, 994, 1338
AflIII ACRYGT 1 cut(s) 1158
AgsI TTSAA 5 cut(s) 20, 32, 58, 554, 1150
AhdI GACNNNNNGTC 2 cut(s) 313, 715
AjnI CCWGG 3 cut(s) 197, 971, 987
AjuI GAANNNNNNNTTGG 2 cut(s) 1041, 1073
Alw21I GWGCWC 1 cut(s) 1020
Alw26I GTCTC 1 cut(s) 1430
Alw44I GTGCAC 1 cut(s) 1016
AlwNI CAGNNNCTG 1 cut(s) 1042
Aor13HI TCCGGA 1 cut(s) 304
AoxI GGCC 4 cut(s) 207, 1091, 1268, 1386
ApaLI GTGCAC 1 cut(s) 1016
ApeKI GCWGC 4 cut(s) 260, 272, 611, 749
ApoI RAATTY 3 cut(s) 26, 555, 729
AspLEI GCGC 2 cut(s) 1246, 1309
AspS9I GGNCC 3 cut(s) 208, 307, 709
AsuC2I CCSGG 1 cut(s) 1124
AsuHPI GGTGA 1 cut(s) 506
AvaII GGWCC 2 cut(s) 307, 709
BaeGI GKGCMC 2 cut(s) 154, 1020
BalI TGGCCA 1 cut(s) 1093
BanII GRGCYC 1 cut(s) 1350
BarI GAAGNNNNNNTAC 2 cut(s) 1427, 1459
Bbv12I GWGCWC 1 cut(s) 1020
BbvI GCAGC 4 cut(s) 247, 259, 623, 761
BccI CCATC 3 cut(s) 104, 384, 674
BciT130I CCWGG 3 cut(s) 199, 973, 989
BcnI CCSGG 1 cut(s) 1124
BcoDI GTCTC 1 cut(s) 1430
BfaI CTAG 3 cut(s) 659, 1080, 1133
BfoI RGCGCY 1 cut(s) 1247
BfuAI ACCTGC 1 cut(s) 951
BisI GCNGC 7 cut(s) 261, 273, 444, 612, 750, 915, 1389
BlsI GCNGC 7 cut(s) 262, 274, 445, 613, 751, 916, 1390
Bme1390I CCNGG 4 cut(s) 199, 973, 989, 1124
Bme18I GGWCC 2 cut(s) 307, 709
BmeRI GACNNNNNGTC 2 cut(s) 313, 715
BmgT120I GGNCC 3 cut(s) 208, 307, 709
BmiI GGNNCC 4 cut(s) 309, 986, 1349, 1402
BmrFI CCNGG 4 cut(s) 199, 973, 989, 1124
BmrI ACTGGG 1 cut(s) 305
BmsI GCATC 3 cut(s) 176, 957, 1287
BmuI ACTGGG 1 cut(s) 305
BpmI CTGGAG 2 cut(s) 105, 201
Bpu10I CCTNAGC 1 cut(s) 1358
BpuMI CCSGG 1 cut(s) 1124
BsaI GGTCTC 1 cut(s) 1430
BsaJI CCNNGG 5 cut(s) 198, 971, 972, 1212, 1383
BsaWI WCCGGW 1 cut(s) 304
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 3 cut(s) 317, 994, 1338
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 6 cut(s) 102, 133, 311, 712, 1052, 1271
Bse3DI GCAATG 4 cut(s) 210, 1206, 1224, 1302
BseAI TCCGGA 1 cut(s) 304
BseBI CCWGG 3 cut(s) 199, 973, 989
BseDI CCNNGG 5 cut(s) 198, 971, 972, 1212, 1383
BseGI GGATG 6 cut(s) 109, 395, 399, 685, 741, 1181
BseLI CCNNNNNNNGG 3 cut(s) 317, 994, 1338
BseMI GCAATG 4 cut(s) 210, 1206, 1224, 1302
BseMII CTCAG 1 cut(s) 1349
BseNI ACTGG 6 cut(s) 102, 133, 311, 712, 1052, 1271
BseRI GAGGAG 1 cut(s) 699
BseSI GKGCMC 2 cut(s) 154, 1020
BseXI GCAGC 4 cut(s) 247, 259, 623, 761
BsgI GTGCAG 1 cut(s) 235
Bsh1236I CGCG 2 cut(s) 917, 1391
BshFI GGCC 4 cut(s) 209, 1093, 1270, 1388
BsiHKAI GWGCWC 1 cut(s) 1020
BsiSI CCGG 5 cut(s) 65, 228, 305, 753, 1124
BslFI GGGAC 1 cut(s) 1271
BslI CCNNNNNNNGG 3 cut(s) 317, 994, 1338
BsmAI GTCTC 1 cut(s) 1430
BsmFI GGGAC 1 cut(s) 1271
BsmI GAATGC 1 cut(s) 977
BsnI GGCC 4 cut(s) 209, 1093, 1270, 1388
Bso31I GGTCTC 1 cut(s) 1430
Bsp1286I GDGCHC 3 cut(s) 154, 1020, 1350
Bsp13I TCCGGA 1 cut(s) 304
Bsp143I GATC 1 cut(s) 666
Bsp19I CCATGG 1 cut(s) 1212
BspACI CCGC 5 cut(s) 441, 444, 707, 915, 1389
BspANI GGCC 4 cut(s) 209, 1093, 1270, 1388
BspCNI CTCAG 1 cut(s) 1350
BspEI TCCGGA 1 cut(s) 304
BspFNI CGCG 2 cut(s) 917, 1391
BspHI TCATGA 1 cut(s) 888
BspLI GGNNCC 4 cut(s) 309, 986, 1349, 1402
BspMI ACCTGC 1 cut(s) 951
BspQI GCTCTTC 1 cut(s) 1448
BspTNI GGTCTC 1 cut(s) 1430
BsrDI GCAATG 4 cut(s) 210, 1206, 1224, 1302
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 6 cut(s) 102, 133, 311, 712, 1052, 1271
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 5 cut(s) 198, 971, 972, 1212, 1383
BssMI GATC 1 cut(s) 666
BssT1I CCWWGG 2 cut(s) 1212, 1383
Bst2UI CCWGG 3 cut(s) 199, 973, 989
Bst4CI ACNGT 4 cut(s) 136, 1044, 1074, 1204
Bst6I CTCTTC 3 cut(s) 839, 890, 1448
BstC8I GCNNGC 9 cut(s) 221, 225, 229, 265, 609, 772, 948, 1242, 1373
BstDEI CTNAG 4 cut(s) 283, 603, 663, 1358
BstDSI CCRYGG 1 cut(s) 1212
BstF5I GGATG 6 cut(s) 109, 395, 399, 685, 741, 1181
BstFNI CGCG 2 cut(s) 917, 1391
BstH2I RGCGCY 1 cut(s) 1247
BstHHI GCGC 2 cut(s) 1246, 1309
BstKTI GATC 1 cut(s) 669
BstMAI GTCTC 1 cut(s) 1430
BstMBI GATC 1 cut(s) 666
BstMWI GCNNNNNNNGC 4 cut(s) 269, 604, 1306, 1368
BstNI CCWGG 3 cut(s) 199, 973, 989
BstNSI RCATGY 2 cut(s) 514, 1162
BstSCI CCNGG 4 cut(s) 197, 971, 987, 1122
BstSLI GKGCMC 2 cut(s) 154, 1020
BstUI CGCG 2 cut(s) 917, 1391
BstV1I GCAGC 4 cut(s) 247, 259, 623, 761
BsuRI GGCC 4 cut(s) 209, 1093, 1270, 1388
BtgI CCRYGG 1 cut(s) 1212
BtgZI GCGATG 2 cut(s) 467, 804
BtsCI GGATG 6 cut(s) 109, 395, 399, 685, 741, 1181
BtsIMutI CAGTG 3 cut(s) 318, 809, 1059
BveI ACCTGC 1 cut(s) 951
Cac8I GCNNGC 9 cut(s) 221, 225, 229, 265, 609, 772, 948, 1242, 1373
CaiI CAGNNNCTG 1 cut(s) 1042
CciI TCATGA 1 cut(s) 888
CfoI GCGC 2 cut(s) 1246, 1309
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 3 cut(s) 208, 307, 709
Csp6I GTAC 4 cut(s) 73, 1040, 1264, 1435
CspCI CAANNNNNGTGG 2 cut(s) 367, 402
CviAII CATG 8 cut(s) 35, 507, 511, 616, 889, 1159, 1173, 1213
CviQI GTAC 4 cut(s) 73, 1040, 1264, 1435
DdeI CTNAG 4 cut(s) 283, 603, 663, 1358
DpnI GATC 1 cut(s) 668
DpnII GATC 1 cut(s) 666
DraI TTTAAA 1 cut(s) 405
DriI GACNNNNNGTC 2 cut(s) 313, 715
EaeI YGGCCR 2 cut(s) 1091, 1386
Eam1104I CTCTTC 3 cut(s) 839, 890, 1448
Eam1105I GACNNNNNGTC 2 cut(s) 313, 715
EarI CTCTTC 3 cut(s) 839, 890, 1448
EciI GGCGGA 1 cut(s) 722
Eco130I CCWWGG 2 cut(s) 1212, 1383
Eco24I GRGCYC 1 cut(s) 1350
Eco31I GGTCTC 1 cut(s) 1430
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 2 cut(s) 307, 709
Eco57I CTGAAG 1 cut(s) 833
EcoRII CCWGG 3 cut(s) 197, 971, 987
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 2 cut(s) 1212, 1383
EcoT22I ATGCAT 1 cut(s) 512
EcoT38I GRGCYC 1 cut(s) 1350
ErhI CCWWGG 2 cut(s) 1212, 1383
FaeI CATG 8 cut(s) 38, 510, 514, 619, 892, 1162, 1176, 1216
FaqI GGGAC 1 cut(s) 1271
FatI CATG 8 cut(s) 34, 506, 510, 615, 888, 1158, 1172, 1212
FauNDI CATATG 1 cut(s) 531
Fnu4HI GCNGC 7 cut(s) 261, 273, 444, 612, 750, 915, 1389
FokI GGATG 6 cut(s) 116, 402, 406, 692, 748, 1168
FriOI GRGCYC 1 cut(s) 1350
Fsp4HI GCNGC 7 cut(s) 261, 273, 444, 612, 750, 915, 1389
FspBI CTAG 3 cut(s) 659, 1080, 1133
FspI TGCGCA 1 cut(s) 1308
GlaI GCGC 2 cut(s) 1245, 1308
GluI GCNGC 7 cut(s) 261, 273, 444, 612, 750, 915, 1389
GsuI CTGGAG 2 cut(s) 105, 201
HaeII RGCGCY 1 cut(s) 1247
HaeIII GGCC 4 cut(s) 209, 1093, 1270, 1388
HapII CCGG 5 cut(s) 65, 228, 305, 753, 1124
HhaI GCGC 2 cut(s) 1246, 1309
Hin1II CATG 8 cut(s) 38, 510, 514, 619, 892, 1162, 1176, 1216
Hin6I GCGC 2 cut(s) 1244, 1307
HinP1I GCGC 2 cut(s) 1244, 1307
HincII GTYRAC 1 cut(s) 591
HindII GTYRAC 1 cut(s) 591
HindIII AAGCTT 2 cut(s) 451, 605
HinfI GANTC 5 cut(s) 38, 47, 500, 592, 1318
HpaII CCGG 5 cut(s) 65, 228, 305, 753, 1124
HphI GGTGA 1 cut(s) 506
Hpy166II GTNNAC 2 cut(s) 591, 1018
Hpy188I TCNGA 5 cut(s) 46, 52, 724, 1253, 1323
Hpy188III TCNNGA 3 cut(s) 305, 889, 1357
Hpy8I GTNNAC 2 cut(s) 591, 1018
HpyAV CCTTC 3 cut(s) 265, 1080, 1343
HpyCH4III ACNGT 4 cut(s) 136, 1044, 1074, 1204
HpyF10VI GCNNNNNNNGC 4 cut(s) 269, 604, 1306, 1368
HpyF3I CTNAG 4 cut(s) 283, 603, 663, 1358
Hsp92II CATG 8 cut(s) 38, 510, 514, 619, 892, 1162, 1176, 1216
HspAI GCGC 2 cut(s) 1244, 1307
Kpn2I TCCGGA 1 cut(s) 304
KroI GCCGGC 1 cut(s) 227
KroNI GCCGGC 1 cut(s) 229
Kzo9I GATC 1 cut(s) 666
LguI GCTCTTC 1 cut(s) 1448
LmnI GCTCC 5 cut(s) 176, 686, 990, 1253, 1353
Lsp1109I GCAGC 4 cut(s) 247, 259, 623, 761
LweI GCATC 3 cut(s) 176, 957, 1287
MaeI CTAG 3 cut(s) 659, 1080, 1133
MaeIII GTNAC 2 cut(s) 293, 1198
MalI GATC 1 cut(s) 668
MboI GATC 1 cut(s) 666
MboII GAAGA 8 cut(s) 101, 509, 856, 877, 1095, 1150, 1219, 1435
MhlI GDGCHC 3 cut(s) 154, 1020, 1350
MlsI TGGCCA 1 cut(s) 1093
MluCI AATT 7 cut(s) 26, 555, 645, 729, 1000, 1023, 1102
MluNI TGGCCA 1 cut(s) 1093
MlyI GAGTC 2 cut(s) 32, 586
MmeI TCCRAC 3 cut(s) 517, 543, 1375
MnlI CCTC 5 cut(s) 7, 337, 677, 840, 1281
Mox20I TGGCCA 1 cut(s) 1093
Mph1103I ATGCAT 1 cut(s) 512
MroI TCCGGA 1 cut(s) 304
MroNI GCCGGC 1 cut(s) 227
MscI TGGCCA 1 cut(s) 1093
MseI TTAA 2 cut(s) 404, 1452
MslI CAYNNNNRTG 4 cut(s) 1013, 1211, 1217, 1235
Msp20I TGGCCA 1 cut(s) 1093
MspI CCGG 5 cut(s) 65, 228, 305, 753, 1124
MspR9I CCNGG 4 cut(s) 199, 973, 989, 1124
Mva1269I GAATGC 1 cut(s) 977
MvaI CCWGG 3 cut(s) 199, 973, 989
MvnI CGCG 2 cut(s) 917, 1391
MwoI GCNNNNNNNGC 4 cut(s) 269, 604, 1306, 1368
NaeI GCCGGC 1 cut(s) 229
NciI CCSGG 1 cut(s) 1124
NcoI CCATGG 1 cut(s) 1212
NdeI CATATG 1 cut(s) 531
NdeII GATC 1 cut(s) 666
NgoMIV GCCGGC 1 cut(s) 227
NlaIII CATG 8 cut(s) 38, 510, 514, 619, 892, 1162, 1176, 1216
NlaIV GGNNCC 4 cut(s) 309, 986, 1349, 1402
NmeAIII GCCGAG 2 cut(s) 195, 574
NsbI TGCGCA 1 cut(s) 1308
NsiI ATGCAT 1 cut(s) 512
NspI RCATGY 2 cut(s) 514, 1162
PagI TCATGA 1 cut(s) 888
PasI CCCWGGG 1 cut(s) 972
PciI ACATGT 1 cut(s) 1158
PciSI GCTCTTC 1 cut(s) 1448
PctI GAATGC 1 cut(s) 977
PdiI GCCGGC 1 cut(s) 229
PfeI GAWTC 3 cut(s) 47, 500, 1318
PfoI TCCNGGA 1 cut(s) 987
PkrI GCNGC 7 cut(s) 262, 274, 445, 613, 751, 916, 1390
PleI GAGTC 2 cut(s) 32, 586
PpsI GAGTC 2 cut(s) 32, 586
PscI ACATGT 1 cut(s) 1158
Psp6I CCWGG 3 cut(s) 197, 971, 987
PspGI CCWGG 3 cut(s) 197, 971, 987
PspN4I GGNNCC 4 cut(s) 309, 986, 1349, 1402
PspPI GGNCC 3 cut(s) 208, 307, 709
PstNI CAGNNNCTG 1 cut(s) 1042
RsaI GTAC 4 cut(s) 74, 1041, 1265, 1436
RsaNI GTAC 4 cut(s) 73, 1040, 1264, 1435
RseI CAYNNNNRTG 4 cut(s) 1013, 1211, 1217, 1235
SapI GCTCTTC 1 cut(s) 1448
SaqAI TTAA 2 cut(s) 404, 1452
SatI GCNGC 7 cut(s) 261, 273, 444, 612, 750, 915, 1389
Sau3AI GATC 1 cut(s) 666
Sau96I GGNCC 3 cut(s) 208, 307, 709
SchI GAGTC 2 cut(s) 32, 586
ScrFI CCNGG 4 cut(s) 199, 973, 989, 1124
SduI GDGCHC 3 cut(s) 154, 1020, 1350
SfaNI GCATC 3 cut(s) 176, 957, 1287
SinI GGWCC 2 cut(s) 307, 709
SmiMI CAYNNNNRTG 4 cut(s) 1013, 1211, 1217, 1235
Sse9I AATT 7 cut(s) 26, 555, 645, 729, 1000, 1023, 1102
SsiI CCGC 5 cut(s) 441, 444, 707, 915, 1389
SspMI CTAG 3 cut(s) 659, 1080, 1133
StyD4I CCNGG 4 cut(s) 197, 971, 987, 1122
StyI CCWWGG 2 cut(s) 1212, 1383
TaaI ACNGT 4 cut(s) 136, 1044, 1074, 1204
TaqI TCGA 2 cut(s) 473, 1068
TasI AATT 7 cut(s) 26, 555, 645, 729, 1000, 1023, 1102
TauI GCSGC 3 cut(s) 446, 917, 1391
TfiI GAWTC 3 cut(s) 47, 500, 1318
Tru1I TTAA 2 cut(s) 404, 1452
Tru9I TTAA 2 cut(s) 404, 1452
TscAI CASTG 3 cut(s) 318, 816, 1059
TseI GCWGC 4 cut(s) 260, 272, 611, 749
TspDTI ATGAA 5 cut(s) 226, 857, 877, 1096, 1157
TspRI CASTG 3 cut(s) 318, 816, 1059
VneI GTGCAC 1 cut(s) 1016
VpaK11BI GGWCC 2 cut(s) 307, 709
XapI RAATTY 3 cut(s) 26, 555, 729
XceI RCATGY 2 cut(s) 514, 1162
XspI CTAG 3 cut(s) 659, 1080, 1133
Zsp2I ATGCAT 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.