MD08G1110600.v1.1

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005525
Physical Location & Seq
Reverse (-)
9724144 .. 9726797
2654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1110600.v1.1.491

Sequence Viewer

Length: 1251 bp
ATGGCTCAACTTGGCTGGCTTGGTGGCATTTCCATTCTCTTGTTCTGCACAGTTGGTGCACTTTATGCTGCTACTCTCTTAGCAGATTGCTACAGATTCCCAGACCCAGTCACTGGCAAGAGAAATTACTCTTACATGGAGGCTGTCAAAGCCTATTTAGGTGGAACAATTTACAAGTTCTGTGGATGGATGATATATTTAAATCTTGCTACCATTGGAGTCGGCTTTACGATAACTACGGCAAAGAGCATGATAGCCATACAGAAATCGAATTGCCATCGCAAACATGGTAGTGATGACCCATGCAAGTTCTCCAACATTCCACATGTGATTGCATTTGGAATTGTAGAAATTCTCTTATCACAACTCCCAAATTTTCACAAGCTCTCTTGGCTCTCAAAGCTTGCAGCAATTATGTCTTTTGGGTATGCTTTTATCGGAATTGGACTTTCCATGTCAAAAATCATAGCAGGGCATGGAGGAAAAACTTCTCTTGCAGGAGCGGACCTGCCTTTATCTGAAAAAATTTGGAGGATGTTTGCAGCAGCTGGAGATATTGCCTTTGCTTGCTCATATGCTCTAGTGCTTTTGGATGTCCAAGACACTCTAAAGTCATCGCCACCAGAAAACAAAGTGATGAAGAAGGCTGTGTCAGTAGGGGGTTTGGTTATGTTAATATTATTCACGATGTGTGGTACGTTGGGATATGCTGCATTTGGAGATAAAACCCCAGAAAACCTACTGGCTGGTTTTGGAGATGATGTAGCCTTCTGGCTTGTTGATATGGCTAATGTTTTCATTGTGGTGCATATAGTTGGTGCATATCAGGTACTTTGCCAACCGGTGTTTCGTATTATTGAACTATTGGCTAGAAGGAGGTGGCCGAAGTCCAAGTTCATAAATAGGGAGATCCCAATCAGACTAGGGAAGGTAAAGTTCAACATCAACATGTTTAGGCTGTCATGGAGGACAGCCTATGGGGTGTTGGTCTCGTTTCTTGCCATAGCTCTTCCATTCTTCTCCGACATGCTTGGCCTTCTTGGAGCCCTGGGCTACTGGCCACTCATAGTGTACATTCCGTTGGAGATGCACATTGTGCAGAACAAGATTGGGAAATGTACAATTAGATGGTTTGGGCTCCAATTGTTTAGTTTTTTGTGCTTGCTTCTTTCTATGGCTGCAGCATCTGGTGCCATTCATGGTCTGTATAAGGGTCTCAAAGCCTACAAGGTCTTCCAATTTAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

417

Amino Acids

45.89

Weight (kDa)

9.46

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 1 - 402 1.1e-87 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 516
AccB1I GGYRCC 1 cut(s) 1190
AccB7I CCANNNNNTGG 1 cut(s) 113
AccBSI CCGCTC 1 cut(s) 503
AciI CCGC 1 cut(s) 503
AclWI GGATC 1 cut(s) 904
AcoI YGGCCR 2 cut(s) 881, 1058
AcsI RAATTY 3 cut(s) 351, 373, 525
AdeI CACNNNGTG 2 cut(s) 690, 1096
AfaI GTAC 4 cut(s) 697, 831, 1073, 1120
AfiI CCNNNNNNNGG 1 cut(s) 113
AflIII ACRYGT 2 cut(s) 325, 948
AgeI ACCGGT 1 cut(s) 841
AgsI TTSAA 2 cut(s) 860, 940
AjnI CCWGG 1 cut(s) 1047
AjuI GAANNNNNNNTTGG 2 cut(s) 831, 863
AluBI AGCT 4 cut(s) 385, 403, 548, 1007
AluI AGCT 4 cut(s) 385, 403, 548, 1007
Alw21I GWGCWC 1 cut(s) 61
Alw26I GTCTC 2 cut(s) 994, 1220
Alw44I GTGCAC 1 cut(s) 57
AlwI GGATC 1 cut(s) 904
AlwNI CAGNNNCTG 3 cut(s) 113, 548, 1187
AoxI GGCC 3 cut(s) 881, 1033, 1058
ApaLI GTGCAC 1 cut(s) 57
ApeKI GCWGC 7 cut(s) 68, 407, 542, 545, 710, 1178, 1181
ApoI RAATTY 3 cut(s) 351, 373, 525
AsiGI ACCGGT 1 cut(s) 841
Asp700I GAANNNNTTC 1 cut(s) 487
AspS9I GGNCC 1 cut(s) 505
AvaII GGWCC 1 cut(s) 505
BaeGI GKGCMC 1 cut(s) 61
BalI TGGCCA 1 cut(s) 1060
BanI GGYRCC 1 cut(s) 1190
BanII GRGCYC 2 cut(s) 1048, 1140
BbsI GAAGAC 1 cut(s) 1225
Bbv12I GWGCWC 1 cut(s) 61
BbvI GCAGC 7 cut(s) 55, 419, 554, 557, 697, 1165, 1193
BccI CCATC 3 cut(s) 180, 285, 1122
BceAI ACGGC 1 cut(s) 255
BciT130I CCWGG 1 cut(s) 1049
BcoDI GTCTC 2 cut(s) 994, 1220
BfaI CTAG 3 cut(s) 581, 870, 923
BfmI CTRYAG 2 cut(s) 91, 1179
BfuAI ACCTGC 1 cut(s) 516
BisI GCNGC 7 cut(s) 69, 408, 543, 546, 711, 1179, 1182
BlsI GCNGC 7 cut(s) 70, 409, 544, 547, 712, 1180, 1183
Bme1390I CCNGG 1 cut(s) 1049
Bme18I GGWCC 1 cut(s) 505
BmgT120I GGNCC 1 cut(s) 505
BmiI GGNNCC 3 cut(s) 1045, 1139, 1192
BmrFI CCNGG 1 cut(s) 1049
BmrI ACTGGG 1 cut(s) 101
BmsI GCATC 2 cut(s) 1077, 1193
BmuI ACTGGG 1 cut(s) 101
BpiI GAAGAC 1 cut(s) 1225
BpmI CTGGAG 1 cut(s) 570
BsaBI GATNNNNATC 1 cut(s) 914
BsaI GGTCTC 2 cut(s) 994, 1220
BsaJI CCNNGG 2 cut(s) 1047, 1048
BsaWI WCCGGW 1 cut(s) 841
BsaXI ACNNNNNCTCC 2 cut(s) 747, 777
Bsc4I CCNNNNNNNGG 1 cut(s) 113
Bse118I RCCGGY 1 cut(s) 841
Bse1I ACTGG 4 cut(s) 107, 118, 747, 1061
Bse8I GATNNNNATC 1 cut(s) 914
BseBI CCWGG 1 cut(s) 1049
BseDI CCNNGG 2 cut(s) 1047, 1048
BseGI GGATG 4 cut(s) 191, 195, 540, 598
BseJI GATNNNNATC 1 cut(s) 914
BseLI CCNNNNNNNGG 1 cut(s) 113
BseNI ACTGG 4 cut(s) 107, 118, 747, 1061
BseSI GKGCMC 1 cut(s) 61
BseXI GCAGC 7 cut(s) 55, 419, 554, 557, 697, 1165, 1193
BsgI GTGCAG 2 cut(s) 31, 1118
BshFI GGCC 3 cut(s) 883, 1035, 1060
BshNI GGYRCC 1 cut(s) 1190
BshTI ACCGGT 1 cut(s) 841
BsiHKAI GWGCWC 1 cut(s) 61
BsiSI CCGG 1 cut(s) 842
BslI CCNNNNNNNGG 1 cut(s) 113
BsmAI GTCTC 2 cut(s) 994, 1220
BsnI GGCC 3 cut(s) 883, 1035, 1060
Bso31I GGTCTC 2 cut(s) 994, 1220
Bsp1286I GDGCHC 3 cut(s) 61, 1048, 1140
Bsp1407I TGTACA 2 cut(s) 1071, 1118
Bsp143I GATC 1 cut(s) 909
BspACI CCGC 1 cut(s) 503
BspANI GGCC 3 cut(s) 883, 1035, 1060
BspLI GGNNCC 3 cut(s) 1045, 1139, 1192
BspMAI CTGCAG 1 cut(s) 1183
BspMI ACCTGC 1 cut(s) 516
BspPI GGATC 1 cut(s) 904
BspQI GCTCTTC 1 cut(s) 1014
BspT107I GGYRCC 1 cut(s) 1190
BspTNI GGTCTC 2 cut(s) 994, 1220
BsrBI CCGCTC 1 cut(s) 503
BsrFI RCCGGY 1 cut(s) 841
BsrGI TGTACA 2 cut(s) 1071, 1118
BsrI ACTGG 4 cut(s) 107, 118, 747, 1061
BssAI RCCGGY 1 cut(s) 841
BssECI CCNNGG 2 cut(s) 1047, 1048
BssMI GATC 1 cut(s) 909
Bst2UI CCWGG 1 cut(s) 1049
Bst4CI ACNGT 1 cut(s) 52
Bst6I CTCTTC 1 cut(s) 1014
BstAPI GCANNNNNTGC 3 cut(s) 65, 1096, 1190
BstAUI TGTACA 2 cut(s) 1071, 1118
BstC8I GCNNGC 4 cut(s) 17, 405, 568, 1163
BstDEI CTNAG 1 cut(s) 79
BstF5I GGATG 4 cut(s) 191, 195, 540, 598
BstKTI GATC 1 cut(s) 912
BstMAI GTCTC 2 cut(s) 994, 1220
BstMBI GATC 1 cut(s) 909
BstMWI GCNNNNNNNGC 6 cut(s) 65, 149, 391, 400, 1096, 1190
BstNI CCWGG 1 cut(s) 1049
BstNSI RCATGY 3 cut(s) 329, 952, 1030
BstSCI CCNGG 1 cut(s) 1047
BstSFI CTRYAG 2 cut(s) 91, 1179
BstSLI GKGCMC 1 cut(s) 61
BstV1I GCAGC 7 cut(s) 55, 419, 554, 557, 697, 1165, 1193
BstV2I GAAGAC 1 cut(s) 1225
BstX2I RGATCY 1 cut(s) 909
BstYI RGATCY 1 cut(s) 909
BsuRI GGCC 3 cut(s) 883, 1035, 1060
BtgZI GCGATG 2 cut(s) 263, 600
BtsCI GGATG 4 cut(s) 191, 195, 540, 598
BtsIMutI CAGTG 1 cut(s) 111
BveI ACCTGC 1 cut(s) 516
Cac8I GCNNGC 4 cut(s) 17, 405, 568, 1163
CaiI CAGNNNCTG 3 cut(s) 113, 548, 1187
Cfr10I RCCGGY 1 cut(s) 841
Cfr13I GGNCC 1 cut(s) 505
Csp6I GTAC 4 cut(s) 696, 830, 1072, 1119
CspAI ACCGGT 1 cut(s) 841
CspCI CAANNNNNGTGG 2 cut(s) 163, 198
CviQI GTAC 4 cut(s) 696, 830, 1072, 1119
DdeI CTNAG 1 cut(s) 79
DpnI GATC 1 cut(s) 911
DpnII GATC 1 cut(s) 909
DraI TTTAAA 2 cut(s) 201, 1243
DraIII CACNNNGTG 2 cut(s) 690, 1096
EaeI YGGCCR 2 cut(s) 881, 1058
Eam1104I CTCTTC 1 cut(s) 1014
EarI CTCTTC 1 cut(s) 1014
Eco24I GRGCYC 2 cut(s) 1048, 1140
Eco31I GGTCTC 2 cut(s) 994, 1220
Eco47I GGWCC 1 cut(s) 505
EcoRII CCWGG 1 cut(s) 1047
EcoT38I GRGCYC 2 cut(s) 1048, 1140
FauNDI CATATG 1 cut(s) 574
Fnu4HI GCNGC 7 cut(s) 69, 408, 543, 546, 711, 1179, 1182
FokI GGATG 4 cut(s) 198, 202, 547, 605
FriOI GRGCYC 2 cut(s) 1048, 1140
Fsp4HI GCNGC 7 cut(s) 69, 408, 543, 546, 711, 1179, 1182
FspBI CTAG 3 cut(s) 581, 870, 923
GluI GCNGC 7 cut(s) 69, 408, 543, 546, 711, 1179, 1182
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 3 cut(s) 883, 1035, 1060
HapII CCGG 1 cut(s) 842
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 2 cut(s) 96, 219
HpaII CCGG 1 cut(s) 842
Hpy166II GTNNAC 2 cut(s) 59, 1072
Hpy188I TCNGA 4 cut(s) 440, 520, 920, 1024
Hpy188III TCNNGA 1 cut(s) 685
Hpy8I GTNNAC 2 cut(s) 59, 1072
HpyAV CCTTC 5 cut(s) 637, 778, 867, 922, 1046
HpyCH4III ACNGT 1 cut(s) 52
HpyCH4IV ACGT 1 cut(s) 698
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 149, 391, 400, 1096, 1190
HpyF3I CTNAG 1 cut(s) 79
HpySE526I ACGT 1 cut(s) 698
Kzo9I GATC 1 cut(s) 909
LguI GCTCTTC 1 cut(s) 1014
LmnI GCTCC 3 cut(s) 500, 1043, 1143
Lsp1109I GCAGC 7 cut(s) 55, 419, 554, 557, 697, 1165, 1193
LweI GCATC 2 cut(s) 1077, 1193
MaeI CTAG 3 cut(s) 581, 870, 923
MaeII ACGT 1 cut(s) 698
MaeIII GTNAC 1 cut(s) 109
MalI GATC 1 cut(s) 911
MbiI CCGCTC 1 cut(s) 503
MboI GATC 1 cut(s) 909
MboII GAAGA 4 cut(s) 652, 1001, 1009, 1225
MfeI CAATTG 1 cut(s) 1142
MflI RGATCY 1 cut(s) 909
MhlI GDGCHC 3 cut(s) 61, 1048, 1140
MlsI TGGCCA 1 cut(s) 1060
MluNI TGGCCA 1 cut(s) 1060
MlyI GAGTC 1 cut(s) 228
MmeI TCCRAC 3 cut(s) 339, 1047, 1062
MnlI CCTC 5 cut(s) 133, 473, 525, 870, 960
Mox20I TGGCCA 1 cut(s) 1060
MroXI GAANNNNTTC 1 cut(s) 487
MscI TGGCCA 1 cut(s) 1060
MseI TTAA 3 cut(s) 200, 674, 1242
MslI CAYNNNNRTG 3 cut(s) 291, 803, 947
Msp20I TGGCCA 1 cut(s) 1060
MspA1I CMGCKG 1 cut(s) 548
MspI CCGG 1 cut(s) 842
MspR9I CCNGG 1 cut(s) 1049
MunI CAATTG 1 cut(s) 1142
MvaI CCWGG 1 cut(s) 1049
MwoI GCNNNNNNNGC 6 cut(s) 65, 149, 391, 400, 1096, 1190
NdeI CATATG 1 cut(s) 574
NdeII GATC 1 cut(s) 909
NlaIV GGNNCC 3 cut(s) 1045, 1139, 1192
NmuCI GTSAC 1 cut(s) 109
NspI RCATGY 3 cut(s) 329, 952, 1030
PasI CCCWGGG 1 cut(s) 1048
PciI ACATGT 2 cut(s) 325, 948
PciSI GCTCTTC 1 cut(s) 1014
PdmI GAANNNNTTC 1 cut(s) 487
PfeI GAWTC 1 cut(s) 96
PflFI GACNNNGTC 1 cut(s) 107
PflMI CCANNNNNTGG 1 cut(s) 113
PinAI ACCGGT 1 cut(s) 841
PkrI GCNGC 7 cut(s) 70, 409, 544, 547, 712, 1180, 1183
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PscI ACATGT 2 cut(s) 325, 948
Psp6I CCWGG 1 cut(s) 1047
PspGI CCWGG 1 cut(s) 1047
PspN4I GGNNCC 3 cut(s) 1045, 1139, 1192
PspPI GGNCC 1 cut(s) 505
PstI CTGCAG 1 cut(s) 1183
PstNI CAGNNNCTG 3 cut(s) 113, 548, 1187
PsuI RGATCY 1 cut(s) 909
PsyI GACNNNGTC 1 cut(s) 107
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 4 cut(s) 697, 831, 1073, 1120
RsaNI GTAC 4 cut(s) 696, 830, 1072, 1119
RseI CAYNNNNRTG 3 cut(s) 291, 803, 947
SapI GCTCTTC 1 cut(s) 1014
SaqAI TTAA 3 cut(s) 200, 674, 1242
SatI GCNGC 7 cut(s) 69, 408, 543, 546, 711, 1179, 1182
Sau3AI GATC 1 cut(s) 909
Sau96I GGNCC 1 cut(s) 505
SchI GAGTC 1 cut(s) 228
ScrFI CCNGG 1 cut(s) 1049
SduI GDGCHC 3 cut(s) 61, 1048, 1140
SfaNI GCATC 2 cut(s) 1077, 1193
SfcI CTRYAG 2 cut(s) 91, 1179
SinI GGWCC 1 cut(s) 505
SmiI ATTTAAAT 1 cut(s) 201
SmiMI CAYNNNNRTG 3 cut(s) 291, 803, 947
SsiI CCGC 1 cut(s) 503
SspI AATATT 1 cut(s) 678
SspMI CTAG 3 cut(s) 581, 870, 923
StyD4I CCNGG 1 cut(s) 1047
SwaI ATTTAAAT 1 cut(s) 201
TaaI ACNGT 1 cut(s) 52
TaiI ACGT 1 cut(s) 701
TaqI TCGA 1 cut(s) 269
TatI WGTACW 2 cut(s) 1071, 1118
TfiI GAWTC 1 cut(s) 96
Tru1I TTAA 3 cut(s) 200, 674, 1242
Tru9I TTAA 3 cut(s) 200, 674, 1242
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 7 cut(s) 68, 407, 542, 545, 710, 1178, 1181
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 4 cut(s) 653, 787, 886, 1187
TspGWI ACGGA 1 cut(s) 1068
TspRI CASTG 1 cut(s) 118
Tth111I GACNNNGTC 1 cut(s) 107
Van91I CCANNNNNTGG 1 cut(s) 113
VneI GTGCAC 1 cut(s) 57
VpaK11BI GGWCC 1 cut(s) 505
XapI RAATTY 3 cut(s) 351, 373, 525
XceI RCATGY 3 cut(s) 329, 952, 1030
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
XmnI GAANNNNTTC 1 cut(s) 487
XspI CTAG 3 cut(s) 581, 870, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.