Rw6G040400

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0005525
Physical Location & Seq
Forward (+)
64207909 .. 64226048
18140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040400.1

Sequence Viewer

Length: 1536 bp
ATGTGTTCTTCATCATTTTCTAGTAATCTCTGTGATTCCGAGTACACCGAATTACATGCAAAGCTAGTTGTCATGGCGTTGAGTGAGGTTGAGACTGAATTTCAATATGACTTATCTGAATCTGGTTTGAACAATCCGGCGAAGTACGGGGAAGCTGGAGAAGAACTACAGGTGGATGATGATGGCAGACTCAAAAGGACTGGAACGGTATGGACTGCCTGTGCCCATATAATCACAGCATCAATAGGAGCTGGAGTGCTATCTCTAGCCTGGGCAATGGCCCAACTCGGATGGCTTGCCGGCATTTTCATTCTCTCGTTCTGCACCTTAGCTGCTGGCTACGCTTCCACTCTCTTAGCAGATTGTTACAGATTTCCGGACCCAGTGTCAGGCAAGAGAAACTACTCCTATATGGAGGCTGTCAAAGTCTATTTAGGTGGAACAATGCACAAGATTTGTGGATGGATGTTATATTTGAACCTTGCTACCATTGGCGTTGGCTTCACTATAACCACCGCAAAAAGCTTGGTGGCTATACAGAAGTCGAGTTGTCATCGCAAAAATGGTGCAGATTCTCCATGCATGTTCTCCAACATTCCACATATGGTTGGATTTGGGCTTGTTGAAATTCTCTTATCACAACTCCAAAACTTTCACAAGTTGACTTGGCTCTCTAAGCTTGCAGCCATGATGTCTTTTGTCTATGCAACTATTGGAATTGGACTTTCTCTAGCTAAGATCATATCAGGGGATGGAGGAACAACATATCTTGCAGGGGCGGACCAGTCGTCATCGGAAAAAATTTGGATGATGTTTATAGCAGCCGGAGATATTGCATTTGCTTGCTCGTATTCTCTTATCCTGTTTGATATCCAAGACACACTGAAGTCATCGCCACCAGAGAACAAAGTGATGAAGAGGGCTGTCGTTATAGGGGGTTTGACTATGGCATTACTCTTCATGATGTGTGGTAGCTTGGGTTATGCTGCGTTTGGCAACATCACACCCGAAAACCTGCTTGCTGGTTTTGGAGATGAGATGCCCTGGGCATTCTGGCTCCTGGATTTGGCTAATTTTTGCATTGTGGTGCACATAGTCGGTGCATTTCAGGTACTGTGCCAACCAGTGTTTCGCATTGTCGAACTGTTGGCTAGAAGAAGGTGGCCAAAATCCAATTTCATTACCAAAGAGAACCTGGTCATACTAGGGAAGATGAAGTTCAATATAAACATGTTTAGGCTGACATGGAGAACATCCTTTGTGGTGCTGGTGACTGTCATTGCCATGGCAATGCCATTCTTCACAGATATGCTGGCGCTTCTCGGAGCTATCGGGTACTGGCCTCTTATAGTTTTTTATGTCCCAATAGAGATGCATGTTGTGCAAAACAAGATTCAGAAACAAACCCTTCGCTGGTTGGGGCTCCAACTCCTGAGCTTTTTGTGCTTGATTCTTTCCTTGGCCGCGGCATCTGGTTCCATTTATGGTCTGCATAAGGGTCTCGGTGCGTGCAATCTCTTCAAAGTTAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

511

Amino Acids

56.1

Weight (kDa)

8.11

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 66 - 497 1.8e-98 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1023
AccII CGCG 1 cut(s) 1466
AccIII TCCGGA 1 cut(s) 376
AciI CCGC 4 cut(s) 516, 779, 1464, 1466
AcoI YGGCCR 2 cut(s) 1163, 1461
AcsI RAATTY 3 cut(s) 98, 627, 801
AcuI CTGAAG 1 cut(s) 905
AfaI GTAC 4 cut(s) 44, 146, 1113, 1337
AfiI CCNNNNNNNGG 3 cut(s) 389, 1066, 1413
AflIII ACRYGT 1 cut(s) 1230
AgsI TTSAA 6 cut(s) 104, 130, 478, 626, 1222, 1522
AhdI GACNNNNNGTC 2 cut(s) 385, 787
AjnI CCWGG 4 cut(s) 269, 1043, 1059, 1194
AjuI GAANNNNNNNTTGG 2 cut(s) 1113, 1145
Alw21I GWGCWC 1 cut(s) 1092
Alw26I GTCTC 2 cut(s) 86, 1505
Alw44I GTGCAC 1 cut(s) 1088
AlwNI CAGNNNCTG 1 cut(s) 1114
Aor13HI TCCGGA 1 cut(s) 376
AoxI GGCC 4 cut(s) 279, 1163, 1340, 1461
ApaLI GTGCAC 1 cut(s) 1088
ApeKI GCWGC 4 cut(s) 332, 683, 821, 986
ApoI RAATTY 3 cut(s) 98, 627, 801
AspLEI GCGC 1 cut(s) 1318
AspS9I GGNCC 3 cut(s) 280, 379, 781
AsuHPI GGTGA 1 cut(s) 1282
AvaII GGWCC 2 cut(s) 379, 781
BaeGI GKGCMC 2 cut(s) 226, 1092
BalI TGGCCA 1 cut(s) 1165
BanII GRGCYC 1 cut(s) 1425
Bbv12I GWGCWC 1 cut(s) 1092
BbvI GCAGC 4 cut(s) 319, 695, 833, 973
BccI CCATC 4 cut(s) 176, 285, 456, 746
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 4 cut(s) 271, 1045, 1061, 1196
BcoDI GTCTC 2 cut(s) 86, 1505
BfaI CTAG 6 cut(s) 21, 65, 266, 731, 1152, 1205
BfmI CTRYAG 1 cut(s) 167
BfoI RGCGCY 1 cut(s) 1319
BfuAI ACCTGC 1 cut(s) 1023
BisI GCNGC 6 cut(s) 333, 684, 822, 987, 1464, 1467
BlsI GCNGC 6 cut(s) 334, 685, 823, 988, 1465, 1468
Bme1390I CCNGG 4 cut(s) 271, 1045, 1061, 1196
Bme18I GGWCC 2 cut(s) 379, 781
BmeRI GACNNNNNGTC 2 cut(s) 385, 787
BmgT120I GGNCC 3 cut(s) 280, 379, 781
BmiI GGNNCC 4 cut(s) 381, 1058, 1424, 1477
BmrFI CCNGG 4 cut(s) 271, 1045, 1061, 1196
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 4 cut(s) 248, 1029, 1362, 1478
BmuI ACTGGG 1 cut(s) 377
BpmI CTGGAG 2 cut(s) 177, 273
Bpu10I CCTNAGC 2 cut(s) 328, 1433
BsaI GGTCTC 1 cut(s) 1505
BsaJI CCNNGG 6 cut(s) 270, 1043, 1044, 1284, 1458, 1464
BsaWI WCCGGW 1 cut(s) 376
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
Bsc4I CCNNNNNNNGG 3 cut(s) 389, 1066, 1413
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 5 cut(s) 205, 383, 784, 1124, 1343
Bse3DI GCAATG 3 cut(s) 282, 1278, 1296
BseAI TCCGGA 1 cut(s) 376
BseBI CCWGG 4 cut(s) 271, 1045, 1061, 1196
BseDI CCNNGG 6 cut(s) 270, 1043, 1044, 1284, 1458, 1464
BseGI GGATG 7 cut(s) 181, 296, 467, 471, 757, 813, 1253
BseLI CCNNNNNNNGG 3 cut(s) 389, 1066, 1413
BseMI GCAATG 3 cut(s) 282, 1278, 1296
BseMII CTCAG 1 cut(s) 1424
BseNI ACTGG 5 cut(s) 205, 383, 784, 1124, 1343
BseSI GKGCMC 2 cut(s) 226, 1092
BseXI GCAGC 4 cut(s) 319, 695, 833, 973
BsgI GTGCAG 2 cut(s) 307, 588
Bsh1236I CGCG 1 cut(s) 1466
BshFI GGCC 4 cut(s) 281, 1165, 1342, 1463
BsiHKAI GWGCWC 1 cut(s) 1092
BsiSI CCGG 4 cut(s) 137, 300, 377, 825
BslFI GGGAC 1 cut(s) 1346
BslI CCNNNNNNNGG 3 cut(s) 389, 1066, 1413
BsmAI GTCTC 2 cut(s) 86, 1505
BsmFI GGGAC 1 cut(s) 1346
BsmI GAATGC 1 cut(s) 1049
BsnI GGCC 4 cut(s) 281, 1165, 1342, 1463
Bso31I GGTCTC 1 cut(s) 1505
Bsp1286I GDGCHC 3 cut(s) 226, 1092, 1425
Bsp13I TCCGGA 1 cut(s) 376
Bsp143I GATC 1 cut(s) 738
Bsp19I CCATGG 1 cut(s) 1284
BspACI CCGC 4 cut(s) 516, 779, 1464, 1466
BspANI GGCC 4 cut(s) 281, 1165, 1342, 1463
BspCNI CTCAG 1 cut(s) 1425
BspEI TCCGGA 1 cut(s) 376
BspFNI CGCG 1 cut(s) 1466
BspHI TCATGA 1 cut(s) 960
BspLI GGNNCC 4 cut(s) 381, 1058, 1424, 1477
BspMI ACCTGC 1 cut(s) 1023
BspTNI GGTCTC 1 cut(s) 1505
BsrDI GCAATG 3 cut(s) 282, 1278, 1296
BsrFI RCCGGY 1 cut(s) 299
BsrI ACTGG 5 cut(s) 205, 383, 784, 1124, 1343
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 6 cut(s) 270, 1043, 1044, 1284, 1458, 1464
BssMI GATC 1 cut(s) 738
BssT1I CCWWGG 2 cut(s) 1284, 1458
Bst2UI CCWGG 4 cut(s) 271, 1045, 1061, 1196
Bst4CI ACNGT 4 cut(s) 208, 1116, 1146, 1276
Bst6I CTCTTC 3 cut(s) 911, 962, 1523
BstAPI GCANNNNNTGC 1 cut(s) 1381
BstC8I GCNNGC 8 cut(s) 297, 301, 337, 681, 844, 1020, 1314, 1510
BstDEI CTNAG 5 cut(s) 328, 355, 675, 735, 1433
BstDSI CCRYGG 2 cut(s) 1284, 1464
BstF5I GGATG 7 cut(s) 181, 296, 467, 471, 757, 813, 1253
BstFNI CGCG 1 cut(s) 1466
BstH2I RGCGCY 1 cut(s) 1319
BstHHI GCGC 1 cut(s) 1318
BstKTI GATC 1 cut(s) 741
BstMAI GTCTC 2 cut(s) 86, 1505
BstMBI GATC 1 cut(s) 738
BstMWI GCNNNNNNNGC 4 cut(s) 341, 676, 1381, 1443
BstNI CCWGG 4 cut(s) 271, 1045, 1061, 1196
BstNSI RCATGY 4 cut(s) 59, 586, 1234, 1379
BstSCI CCNGG 4 cut(s) 269, 1043, 1059, 1194
BstSFI CTRYAG 1 cut(s) 167
BstSLI GKGCMC 2 cut(s) 226, 1092
BstUI CGCG 1 cut(s) 1466
BstV1I GCAGC 4 cut(s) 319, 695, 833, 973
BsuRI GGCC 4 cut(s) 281, 1165, 1342, 1463
BtgI CCRYGG 2 cut(s) 1284, 1464
BtgZI GCGATG 2 cut(s) 539, 876
BtsCI GGATG 7 cut(s) 181, 296, 467, 471, 757, 813, 1253
BtsIMutI CAGTG 3 cut(s) 390, 881, 1131
BveI ACCTGC 1 cut(s) 1023
Cac8I GCNNGC 8 cut(s) 297, 301, 337, 681, 844, 1020, 1314, 1510
CaiI CAGNNNCTG 1 cut(s) 1114
CciI TCATGA 1 cut(s) 960
CfoI GCGC 1 cut(s) 1318
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 3 cut(s) 280, 379, 781
Cfr42I CCGCGG 1 cut(s) 1467
CsiI ACCWGGT 1 cut(s) 1194
Csp6I GTAC 4 cut(s) 43, 145, 1112, 1336
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviQI GTAC 4 cut(s) 43, 145, 1112, 1336
DdeI CTNAG 5 cut(s) 328, 355, 675, 735, 1433
DpnI GATC 1 cut(s) 740
DpnII GATC 1 cut(s) 738
DriI GACNNNNNGTC 2 cut(s) 385, 787
EaeI YGGCCR 2 cut(s) 1163, 1461
Eam1104I CTCTTC 3 cut(s) 911, 962, 1523
Eam1105I GACNNNNNGTC 2 cut(s) 385, 787
EarI CTCTTC 3 cut(s) 911, 962, 1523
EciI GGCGGA 1 cut(s) 794
Eco130I CCWWGG 2 cut(s) 1284, 1458
Eco24I GRGCYC 1 cut(s) 1425
Eco31I GGTCTC 1 cut(s) 1505
Eco32I GATATC 1 cut(s) 871
Eco47I GGWCC 2 cut(s) 379, 781
Eco57I CTGAAG 1 cut(s) 905
EcoRII CCWGG 4 cut(s) 269, 1043, 1059, 1194
EcoRV GATATC 1 cut(s) 871
EcoT14I CCWWGG 2 cut(s) 1284, 1458
EcoT22I ATGCAT 2 cut(s) 584, 1377
EcoT38I GRGCYC 1 cut(s) 1425
ErhI CCWWGG 2 cut(s) 1284, 1458
FaqI GGGAC 1 cut(s) 1346
FauNDI CATATG 1 cut(s) 603
Fnu4HI GCNGC 6 cut(s) 333, 684, 822, 987, 1464, 1467
FokI GGATG 7 cut(s) 188, 303, 474, 478, 764, 820, 1240
FriOI GRGCYC 1 cut(s) 1425
Fsp4HI GCNGC 6 cut(s) 333, 684, 822, 987, 1464, 1467
FspBI CTAG 6 cut(s) 21, 65, 266, 731, 1152, 1205
GlaI GCGC 1 cut(s) 1317
GluI GCNGC 6 cut(s) 333, 684, 822, 987, 1464, 1467
GsuI CTGGAG 2 cut(s) 177, 273
HaeII RGCGCY 1 cut(s) 1319
HaeIII GGCC 4 cut(s) 281, 1165, 1342, 1463
HapII CCGG 4 cut(s) 137, 300, 377, 825
HhaI GCGC 1 cut(s) 1318
Hin6I GCGC 1 cut(s) 1316
HinP1I GCGC 1 cut(s) 1316
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HindIII AAGCTT 2 cut(s) 523, 677
HinfI GANTC 6 cut(s) 35, 119, 189, 572, 1393, 1450
HpaII CCGG 4 cut(s) 137, 300, 377, 825
HphI GGTGA 1 cut(s) 1282
Hpy166II GTNNAC 3 cut(s) 45, 663, 1090
Hpy188I TCNGA 6 cut(s) 40, 118, 290, 796, 1325, 1398
Hpy188III TCNNGA 3 cut(s) 377, 961, 1432
Hpy8I GTNNAC 3 cut(s) 45, 663, 1090
HpyAV CCTTC 2 cut(s) 1152, 1418
HpyCH4III ACNGT 4 cut(s) 208, 1116, 1146, 1276
HpyF10VI GCNNNNNNNGC 4 cut(s) 341, 676, 1381, 1443
HpyF3I CTNAG 5 cut(s) 328, 355, 675, 735, 1433
HspAI GCGC 1 cut(s) 1316
Kpn2I TCCGGA 1 cut(s) 376
KroI GCCGGC 1 cut(s) 299
KroNI GCCGGC 1 cut(s) 301
KspI CCGCGG 1 cut(s) 1467
Kzo9I GATC 1 cut(s) 738
LmnI GCTCC 4 cut(s) 248, 1062, 1325, 1428
Lsp1109I GCAGC 4 cut(s) 319, 695, 833, 973
LweI GCATC 4 cut(s) 248, 1029, 1362, 1478
MabI ACCWGGT 1 cut(s) 1194
MaeI CTAG 6 cut(s) 21, 65, 266, 731, 1152, 1205
MaeIII GTNAC 2 cut(s) 365, 1270
MalI GATC 1 cut(s) 740
MboI GATC 1 cut(s) 738
MboII GAAGA 7 cut(s) 173, 928, 949, 1167, 1222, 1291, 1510
MhlI GDGCHC 3 cut(s) 226, 1092, 1425
MlsI TGGCCA 1 cut(s) 1165
MluCI AATT 7 cut(s) 50, 98, 627, 717, 801, 1072, 1174
MluNI TGGCCA 1 cut(s) 1165
MlyI GAGTC 1 cut(s) 183
MmeI TCCRAC 3 cut(s) 589, 615, 1450
MnlI CCTC 5 cut(s) 79, 409, 749, 912, 1353
Mox20I TGGCCA 1 cut(s) 1165
Mph1103I ATGCAT 2 cut(s) 584, 1377
MroI TCCGGA 1 cut(s) 376
MroNI GCCGGC 1 cut(s) 299
MscI TGGCCA 1 cut(s) 1165
MseI TTAA 1 cut(s) 1527
MslI CAYNNNNRTG 4 cut(s) 1085, 1283, 1289, 1307
Msp20I TGGCCA 1 cut(s) 1165
MspA1I CMGCKG 1 cut(s) 1466
MspI CCGG 4 cut(s) 137, 300, 377, 825
MspR9I CCNGG 4 cut(s) 271, 1045, 1061, 1196
Mva1269I GAATGC 1 cut(s) 1049
MvaI CCWGG 4 cut(s) 271, 1045, 1061, 1196
MvnI CGCG 1 cut(s) 1466
MwoI GCNNNNNNNGC 4 cut(s) 341, 676, 1381, 1443
NaeI GCCGGC 1 cut(s) 301
NcoI CCATGG 1 cut(s) 1284
NdeI CATATG 1 cut(s) 603
NdeII GATC 1 cut(s) 738
NgoMIV GCCGGC 1 cut(s) 299
NlaIV GGNNCC 4 cut(s) 381, 1058, 1424, 1477
NmuCI GTSAC 1 cut(s) 1270
NsiI ATGCAT 2 cut(s) 584, 1377
NspI RCATGY 4 cut(s) 59, 586, 1234, 1379
PagI TCATGA 1 cut(s) 960
PasI CCCWGGG 1 cut(s) 1044
PciI ACATGT 1 cut(s) 1230
PctI GAATGC 1 cut(s) 1049
PdiI GCCGGC 1 cut(s) 301
PfeI GAWTC 5 cut(s) 35, 119, 572, 1393, 1450
PfoI TCCNGGA 1 cut(s) 1059
PkrI GCNGC 6 cut(s) 334, 685, 823, 988, 1465, 1468
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
PscI ACATGT 1 cut(s) 1230
Psp6I CCWGG 4 cut(s) 269, 1043, 1059, 1194
PspGI CCWGG 4 cut(s) 269, 1043, 1059, 1194
PspN4I GGNNCC 4 cut(s) 381, 1058, 1424, 1477
PspPI GGNCC 3 cut(s) 280, 379, 781
PstNI CAGNNNCTG 1 cut(s) 1114
RsaI GTAC 4 cut(s) 44, 146, 1113, 1337
RsaNI GTAC 4 cut(s) 43, 145, 1112, 1336
RseI CAYNNNNRTG 4 cut(s) 1085, 1283, 1289, 1307
SacII CCGCGG 1 cut(s) 1467
SaqAI TTAA 1 cut(s) 1527
SatI GCNGC 6 cut(s) 333, 684, 822, 987, 1464, 1467
Sau3AI GATC 1 cut(s) 738
Sau96I GGNCC 3 cut(s) 280, 379, 781
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 4 cut(s) 271, 1045, 1061, 1196
SduI GDGCHC 3 cut(s) 226, 1092, 1425
SexAI ACCWGGT 1 cut(s) 1194
SfaNI GCATC 4 cut(s) 248, 1029, 1362, 1478
SfcI CTRYAG 1 cut(s) 167
Sfr303I CCGCGG 1 cut(s) 1467
SgrBI CCGCGG 1 cut(s) 1467
SinI GGWCC 2 cut(s) 379, 781
SmiMI CAYNNNNRTG 4 cut(s) 1085, 1283, 1289, 1307
Sse9I AATT 7 cut(s) 50, 98, 627, 717, 801, 1072, 1174
SsiI CCGC 4 cut(s) 516, 779, 1464, 1466
SspMI CTAG 6 cut(s) 21, 65, 266, 731, 1152, 1205
StyD4I CCNGG 4 cut(s) 269, 1043, 1059, 1194
StyI CCWWGG 2 cut(s) 1284, 1458
TaaI ACNGT 4 cut(s) 208, 1116, 1146, 1276
TaqI TCGA 2 cut(s) 545, 1140
TasI AATT 7 cut(s) 50, 98, 627, 717, 801, 1072, 1174
TatI WGTACW 1 cut(s) 42
TauI GCSGC 2 cut(s) 1466, 1469
TfiI GAWTC 5 cut(s) 35, 119, 572, 1393, 1450
Tru1I TTAA 1 cut(s) 1527
Tru9I TTAA 1 cut(s) 1527
TscAI CASTG 3 cut(s) 390, 888, 1131
TseFI GTSAC 1 cut(s) 1270
TseI GCWGC 4 cut(s) 332, 683, 821, 986
Tsp45I GTSAC 1 cut(s) 1270
TspDTI ATGAA 5 cut(s) 298, 929, 949, 1168, 1229
TspRI CASTG 3 cut(s) 390, 888, 1131
VneI GTGCAC 1 cut(s) 1088
VpaK11BI GGWCC 2 cut(s) 379, 781
XapI RAATTY 3 cut(s) 98, 627, 801
XceI RCATGY 4 cut(s) 59, 586, 1234, 1379
XcmI CCANNNNNNNNNTGG 1 cut(s) 1192
XspI CTAG 6 cut(s) 21, 65, 266, 731, 1152, 1205
Zsp2I ATGCAT 2 cut(s) 584, 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.