FvH4_2g39190

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005902
Physical Location & Seq
Reverse (-)
28206999 .. 28210209
3211 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39190.t1

Sequence Viewer

Length: 855 bp
ATGACTAGCACCAGCGTCAACCCGGTTAGCACAGAAATGCAGCCATTGAAGTCTGACGGTGTCCATAAGAAATGTCAGGAGATCAAAACTAGATGTTCGATACACGATCAACCTAAAAGACGAAAATTCATGTTTGACTCAAGTAAAATGAAGCACAAAAAAAAGTCGAAAAACAATGCAAAAACTTCATCAAAGGCACCACTTTCTCTCAAAAAGCACAAGATTGTAGAACAATCTAGAGGGACTTTCGCTGAAGTTGACATAAGCAGTCAAGTGAGACCAAATGCACTAAAGGTGAAGATATCACCACCTCCATCTACACGTCCAAAGACGAGATCAACATCACCATCAACTCAAAGAATCAAGTCTACCCCATCTATTCGTGGGATGAAACGCAAAAATGTATTAATTAGGGAGAAGCCTAGAAATGCCTCTGATGCAGCAAGGAGCGCCGCATATGAGGAGGCTAAAGCTCATCAAATGGGTTTAGATTCTAAAACTCCATCGATTGTTAAATTGATGGTTTATTCGCATGTTTCTAGAGTGTTTTGGTTGGGCCTCCCGAAAGATTTTTGTGATGCGAATCTCGTCAAAAAGAAGTCGGACATAACGTTGCAAGACGAGCATGGCAACCAATTCACTGTTGTCTATATTCCGGAGCGACATGGGCTAAGCGGAGGTTGGAAAAAATTTGCCCAAGAAAAGAAGTTACATGAAGGCGACGCCATAGTGCTTCAAAGAACTGGACCAAAAAGGCTCAAGGTTCATATATTCAGATCAGCAGAACTGCTCCCGAAAATTCAAGCGGAATGTGTAAGCAGCCTTGAGATAAAGAAGAAGCAGCGCAACATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

285

Amino Acids

32.1

Weight (kDa)

10.32

Isoelectric Point (pI)

50.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 171 - 260 2.9e-19 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 368
AccIII TCCGGA 1 cut(s) 655
AciI CCGC 3 cut(s) 453, 675, 806
AclI AACGTT 1 cut(s) 611
AcsI RAATTY 3 cut(s) 125, 689, 798
AcuI CTGAAG 1 cut(s) 273
AcyI GRCGYC 1 cut(s) 723
AflIII ACRYGT 1 cut(s) 320
AgsI TTSAA 3 cut(s) 49, 737, 803
AjiI CACGTC 1 cut(s) 323
AluBI AGCT 1 cut(s) 473
AluI AGCT 1 cut(s) 473
Alw26I GTCTC 1 cut(s) 271
Aor13HI TCCGGA 1 cut(s) 655
AoxI GGCC 1 cut(s) 556
ApeKI GCWGC 4 cut(s) 40, 440, 819, 841
ApoI RAATTY 3 cut(s) 125, 689, 798
AseI ATTAAT 1 cut(s) 407
AspLEI GCGC 2 cut(s) 452, 846
AspS9I GGNCC 2 cut(s) 556, 746
AsuC2I CCSGG 1 cut(s) 23
AsuHPI GGTGA 3 cut(s) 297, 307, 336
AvaII GGWCC 1 cut(s) 746
BanI GGYRCC 1 cut(s) 196
BbvI GCAGC 3 cut(s) 52, 452, 831
BccI CCATC 5 cut(s) 322, 355, 382, 511, 514
BcnI CCSGG 1 cut(s) 23
BcoDI GTCTC 1 cut(s) 271
BfaI CTAG 5 cut(s) 6, 90, 237, 423, 540
BfoI RGCGCY 1 cut(s) 453
BisI GCNGC 5 cut(s) 41, 441, 453, 820, 842
BlpI GCTNAGC 1 cut(s) 671
BlsI GCNGC 5 cut(s) 42, 442, 454, 821, 843
Bme1390I CCNGG 1 cut(s) 23
Bme18I GGWCC 1 cut(s) 746
BmgBI CACGTC 1 cut(s) 323
BmgT120I GGNCC 2 cut(s) 556, 746
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 23
BmsI GCATC 2 cut(s) 427, 568
Bpu1102I GCTNAGC 1 cut(s) 671
BpuEI CTTGAG 3 cut(s) 124, 743, 845
BpuMI CCSGG 1 cut(s) 23
Bsa29I ATCGAT 1 cut(s) 506
BsaBI GATNNNNATC 2 cut(s) 340, 582
BsaHI GRCGYC 1 cut(s) 723
BsaI GGTCTC 1 cut(s) 271
BsaWI WCCGGW 1 cut(s) 655
Bse1I ACTGG 1 cut(s) 748
Bse8I GATNNNNATC 2 cut(s) 340, 582
BseAI TCCGGA 1 cut(s) 655
BseCI ATCGAT 1 cut(s) 506
BseGI GGATG 1 cut(s) 393
BseJI GATNNNNATC 2 cut(s) 340, 582
BseNI ACTGG 1 cut(s) 748
BseRI GAGGAG 1 cut(s) 476
BseXI GCAGC 3 cut(s) 52, 452, 831
BshFI GGCC 1 cut(s) 558
BshNI GGYRCC 1 cut(s) 196
BshVI ATCGAT 1 cut(s) 506
BsiSI CCGG 2 cut(s) 23, 656
BslFI GGGAC 1 cut(s) 256
BsmAI GTCTC 1 cut(s) 271
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 1 cut(s) 558
Bso31I GGTCTC 1 cut(s) 271
Bsp13I TCCGGA 1 cut(s) 655
Bsp143I GATC 4 cut(s) 81, 106, 335, 776
Bsp1720I GCTNAGC 1 cut(s) 671
BspACI CCGC 3 cut(s) 453, 675, 806
BspANI GGCC 1 cut(s) 558
BspDI ATCGAT 1 cut(s) 506
BspEI TCCGGA 1 cut(s) 655
BspLI GGNNCC 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 196
BspTNI GGTCTC 1 cut(s) 271
BsrI ACTGG 1 cut(s) 748
BssMI GATC 4 cut(s) 81, 106, 335, 776
BssNI GRCGYC 1 cut(s) 723
Bst4CI ACNGT 2 cut(s) 59, 643
BstACI GRCGYC 1 cut(s) 723
BstDEI CTNAG 1 cut(s) 671
BstF5I GGATG 1 cut(s) 393
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 2 cut(s) 452, 846
BstKTI GATC 4 cut(s) 84, 109, 338, 779
BstMAI GTCTC 1 cut(s) 271
BstMBI GATC 4 cut(s) 81, 106, 335, 776
BstMWI GCNNNNNNNGC 4 cut(s) 437, 449, 622, 667
BstNSI RCATGY 1 cut(s) 536
BstSCI CCNGG 1 cut(s) 21
BstV1I GCAGC 3 cut(s) 52, 452, 831
Bsu15I ATCGAT 1 cut(s) 506
BsuRI GGCC 1 cut(s) 558
BsuTUI ATCGAT 1 cut(s) 506
BtrI CACGTC 1 cut(s) 323
BtsCI GGATG 1 cut(s) 393
BtsIMutI CAGTG 1 cut(s) 639
CfoI GCGC 2 cut(s) 452, 846
Cfr13I GGNCC 2 cut(s) 556, 746
ClaI ATCGAT 1 cut(s) 506
CseI GACGC 2 cut(s) 4, 731
CviAII CATG 5 cut(s) 130, 533, 626, 665, 713
CviJI RGCY 8 cut(s) 43, 421, 467, 473, 558, 670, 757, 822
CviKI_1 RGCY 8 cut(s) 43, 421, 467, 473, 558, 670, 757, 822
DdeI CTNAG 1 cut(s) 671
DpnI GATC 4 cut(s) 83, 108, 337, 778
DpnII GATC 4 cut(s) 81, 106, 335, 776
Eco31I GGTCTC 1 cut(s) 271
Eco32I GATATC 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 746
Eco57I CTGAAG 1 cut(s) 273
EcoRV GATATC 1 cut(s) 303
FaeI CATG 5 cut(s) 133, 536, 629, 668, 716
FaqI GGGAC 1 cut(s) 256
FatI CATG 5 cut(s) 129, 532, 625, 664, 712
FauNDI CATATG 1 cut(s) 457
FblI GTMKAC 1 cut(s) 368
Fnu4HI GCNGC 5 cut(s) 41, 441, 453, 820, 842
FokI GGATG 1 cut(s) 400
Fsp4HI GCNGC 5 cut(s) 41, 441, 453, 820, 842
FspBI CTAG 5 cut(s) 6, 90, 237, 423, 540
GlaI GCGC 2 cut(s) 451, 845
GluI GCNGC 5 cut(s) 41, 441, 453, 820, 842
HaeII RGCGCY 1 cut(s) 453
HaeIII GGCC 1 cut(s) 558
HapII CCGG 2 cut(s) 23, 656
HgaI GACGC 2 cut(s) 4, 731
HhaI GCGC 2 cut(s) 452, 846
Hin1I GRCGYC 1 cut(s) 723
Hin1II CATG 5 cut(s) 133, 536, 629, 668, 716
Hin6I GCGC 2 cut(s) 450, 844
HinP1I GCGC 2 cut(s) 450, 844
HincII GTYRAC 2 cut(s) 19, 259
HindII GTYRAC 2 cut(s) 19, 259
HinfI GANTC 4 cut(s) 137, 360, 491, 583
HpaII CCGG 2 cut(s) 23, 656
HphI GGTGA 3 cut(s) 297, 307, 336
Hpy166II GTNNAC 3 cut(s) 19, 259, 369
Hpy188I TCNGA 4 cut(s) 55, 436, 604, 776
Hpy188III TCNNGA 6 cut(s) 77, 237, 540, 562, 656, 793
Hpy8I GTNNAC 3 cut(s) 19, 259, 369
Hpy99I CGWCG 1 cut(s) 725
HpyAV CCTTC 1 cut(s) 710
HpyCH4III ACNGT 2 cut(s) 59, 643
HpyCH4IV ACGT 2 cut(s) 322, 611
HpyCH4V TGCA 5 cut(s) 40, 179, 287, 440, 616
HpyF10VI GCNNNNNNNGC 4 cut(s) 437, 449, 622, 667
HpyF3I CTNAG 1 cut(s) 671
HpySE526I ACGT 2 cut(s) 322, 611
Hsp92I GRCGYC 1 cut(s) 723
Hsp92II CATG 5 cut(s) 133, 536, 629, 668, 716
HspAI GCGC 2 cut(s) 450, 844
Kpn2I TCCGGA 1 cut(s) 655
Kzo9I GATC 4 cut(s) 81, 106, 335, 776
LmnI GCTCC 3 cut(s) 447, 658, 795
LpnPI CCDG 5 cut(s) 25, 36, 62, 669, 729
Lsp1109I GCAGC 3 cut(s) 52, 452, 831
LweI GCATC 2 cut(s) 427, 568
MaeI CTAG 5 cut(s) 6, 90, 237, 423, 540
MaeII ACGT 2 cut(s) 322, 611
MaeIII GTNAC 1 cut(s) 708
MalI GATC 4 cut(s) 83, 108, 337, 778
MboI GATC 4 cut(s) 81, 106, 335, 776
MboII GAAGA 2 cut(s) 310, 847
MluCI AATT 6 cut(s) 125, 408, 515, 635, 689, 798
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 2 cut(s) 582, 662
MnlI CCTC 7 cut(s) 233, 321, 442, 454, 457, 569, 671
MroI TCCGGA 1 cut(s) 655
MseI TTAA 2 cut(s) 407, 513
MslI CAYNNNNRTG 1 cut(s) 35
MspI CCGG 2 cut(s) 23, 656
MspR9I CCNGG 1 cut(s) 23
MwoI GCNNNNNNNGC 4 cut(s) 437, 449, 622, 667
NciI CCSGG 1 cut(s) 23
NdeI CATATG 1 cut(s) 457
NdeII GATC 4 cut(s) 81, 106, 335, 776
NlaIII CATG 5 cut(s) 133, 536, 629, 668, 716
NlaIV GGNNCC 1 cut(s) 198
NspI RCATGY 1 cut(s) 536
PcsI WCGNNNNNNNCGW 1 cut(s) 608
PfeI GAWTC 3 cut(s) 360, 491, 583
PflFI GACNNNGTC 1 cut(s) 59
PkrI GCNGC 5 cut(s) 42, 442, 454, 821, 843
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PshBI ATTAAT 1 cut(s) 407
Psp1406I AACGTT 1 cut(s) 611
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 2 cut(s) 556, 746
PsyI GACNNNGTC 1 cut(s) 59
RseI CAYNNNNRTG 1 cut(s) 35
SaqAI TTAA 2 cut(s) 407, 513
SatI GCNGC 5 cut(s) 41, 441, 453, 820, 842
Sau3AI GATC 4 cut(s) 81, 106, 335, 776
Sau96I GGNCC 2 cut(s) 556, 746
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 23
SetI ASST 8 cut(s) 115, 297, 313, 325, 475, 614, 682, 765
SfaNI GCATC 2 cut(s) 427, 568
SinI GGWCC 1 cut(s) 746
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 3 cut(s) 139, 758, 824
SmoI CTYRAG 3 cut(s) 139, 758, 824
Sse9I AATT 6 cut(s) 125, 408, 515, 635, 689, 798
SsiI CCGC 3 cut(s) 453, 675, 806
SspMI CTAG 5 cut(s) 6, 90, 237, 423, 540
StyD4I CCNGG 1 cut(s) 21
TaaI ACNGT 2 cut(s) 59, 643
TaiI ACGT 2 cut(s) 325, 614
TaqI TCGA 3 cut(s) 98, 167, 506
TasI AATT 6 cut(s) 125, 408, 515, 635, 689, 798
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 3 cut(s) 360, 491, 583
Tru1I TTAA 2 cut(s) 407, 513
Tru9I TTAA 2 cut(s) 407, 513
TscAI CASTG 1 cut(s) 646
TseI GCWGC 4 cut(s) 40, 440, 819, 841
TspDTI ATGAA 6 cut(s) 118, 164, 177, 404, 729, 755
TspRI CASTG 1 cut(s) 646
Tth111I GACNNNGTC 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 746
VspI ATTAAT 1 cut(s) 407
XapI RAATTY 3 cut(s) 125, 689, 798
XbaI TCTAGA 2 cut(s) 236, 539
XceI RCATGY 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 368
XspI CTAG 5 cut(s) 6, 90, 237, 423, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.