Rroxscaffold_7G00166320

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005902
Physical Location & Seq
Reverse (-)
7824565 .. 7827413
2849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00166320.1

Sequence Viewer

Length: 957 bp
ATGGCTGCCACCTATGATAGCAAAGAAGTGAAATCTCAGCCACTGAAACCTCGCGGAGTCTATGACGGACCTCAGCTCGCAAAACCCGAAATTTCACTGAGAAAGGAGGAAAACCACCAGAACAAGAATGGTGCTGAAGAAAGAAAGCGGGTTATTAAAACAGTATGCTCAATACATGCTCCTCGTCCGAGGCTGCGATTTACGTGGGACTCCAGTATTAAAAAGGCCCCGAAGAAGAAGTCAAGAACACCATCAGCTCCGAAATCTAGGTCAAGCTCACCGGTTTCTGGGAGAAAGCTCAAGCGTGCCGAGGAACCTATAGTTGCTTCTGCTGAAGTAAGGAACGCTGCTCTCGAATCCGATGTTGAGAAAAGCAGTAATAAGGAACCAACTGCACCGGAGGTGAAGACAACATCACCTCTACCACCAAAAACGAAGTCAGCGGCACCATCAGCTCCAAAAATCAATTCAACACCACCGATGTCTGGGAGAAAACGTGGAAACGCAATAGTCAGGGAGGAACCAAGAGATGCTTCTATTGAAGCAAGGAGTATTGCTCGTGAAGCGGCGAGAGCTCATCAAATGAGTTTAGCTTCTGGAACTCCGTCGGTCGTCATATCAATGGTCCCTTCGCATGTTTATAGAGTATTTTGGTTGAACCTTCCGAGAAGATTTTGTGATGGTAATCTCCCGGAAACTAAATCGAAACTGACGTTAGAGGATGAGGAAGGAAACGAATTCACGGTTGTCTTTATTCCGGAGCGATATGGGCTGAGTGGAGGGTGGGTGAGCTTTGCGCGGGAAAAAAAGTTGCACGACGGCGACGCTGTAGTGCTTGAACAAATTGAAAAAACTAGACTCAAAGTACATATATTTAGATCAGCAGAACTGTTTCCCAATGTTCAAGCGGAGAGTGCACGCAGCCTTGAGAAGAAGAAGAAGCAGCAGCAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

318

Amino Acids

35.4

Weight (kDa)

10.02

Isoelectric Point (pI)

59.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 207 - 294 6.9e-17 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 445
AccII CGCG 2 cut(s) 54, 799
AccIII TCCGGA 1 cut(s) 757
AciI CCGC 6 cut(s) 54, 148, 443, 566, 799, 908
AcsI RAATTY 2 cut(s) 90, 737
AcuI CTGAAG 2 cut(s) 156, 354
AfaI GTAC 1 cut(s) 867
AfiI CCNNNNNNNGG 2 cut(s) 287, 485
AgeI ACCGGT 1 cut(s) 280
AgsI TTSAA 6 cut(s) 471, 542, 658, 839, 848, 905
AjuI GAANNNNNNNTTGG 4 cut(s) 451, 483, 517, 549
AluBI AGCT 8 cut(s) 76, 257, 276, 298, 455, 575, 593, 792
AluI AGCT 8 cut(s) 76, 257, 276, 298, 455, 575, 593, 792
Alw21I GWGCWC 2 cut(s) 577, 919
Alw44I GTGCAC 1 cut(s) 915
AlwNI CAGNNNCTG 1 cut(s) 43
Aor13HI TCCGGA 1 cut(s) 757
AoxI GGCC 1 cut(s) 225
ApaLI GTGCAC 1 cut(s) 915
ApeKI GCWGC 6 cut(s) 5, 193, 347, 921, 943, 946
ApoI RAATTY 2 cut(s) 90, 737
ArsI GACNNNNNNTTYG 6 cut(s) 224, 254, 256, 286, 422, 454
AsiGI ACCGGT 1 cut(s) 280
Asp700I GAANNNNTTC 1 cut(s) 891
AspLEI GCGC 1 cut(s) 799
AspS9I GGNCC 3 cut(s) 68, 226, 625
AsuC2I CCSGG 1 cut(s) 692
AsuHPI GGTGA 4 cut(s) 270, 408, 415, 799
AvaII GGWCC 2 cut(s) 68, 625
BaeGI GKGCMC 1 cut(s) 919
BanI GGYRCC 1 cut(s) 445
BanII GRGCYC 1 cut(s) 577
BauI CACGAG 1 cut(s) 558
BbsI GAAGAC 1 cut(s) 413
Bbv12I GWGCWC 2 cut(s) 577, 919
BbvCI CCTCAGC 1 cut(s) 72
BbvI GCAGC 3 cut(s) 180, 334, 933
BccI CCATC 3 cut(s) 259, 457, 674
BceAI ACGGC 1 cut(s) 835
BcnI CCSGG 1 cut(s) 692
BfaI CTAG 2 cut(s) 267, 855
BfmI CTRYAG 2 cut(s) 318, 828
BglII AGATCT 1 cut(s) 951
BisI GCNGC 8 cut(s) 6, 194, 348, 444, 567, 922, 944, 947
BlsI GCNGC 8 cut(s) 7, 195, 349, 445, 568, 923, 945, 948
Bme1390I CCNGG 1 cut(s) 692
Bme18I GGWCC 2 cut(s) 68, 625
BmgT120I GGNCC 3 cut(s) 68, 226, 625
BmiI GGNNCC 6 cut(s) 228, 315, 387, 447, 522, 627
BmrFI CCNGG 1 cut(s) 692
BmsI GCATC 1 cut(s) 520
BpiI GAAGAC 1 cut(s) 413
BplI GAGNNNNNCTC 2 cut(s) 541, 573
BpmI CTGGAG 1 cut(s) 196
Bpu10I CCTNAGC 1 cut(s) 72
BpuEI CTTGAG 2 cut(s) 284, 947
BpuMI CCSGG 1 cut(s) 692
BsaAI YACGTR 1 cut(s) 204
BsaBI GATNNNNATC 1 cut(s) 684
BsaJI CCNNGG 2 cut(s) 188, 309
BsaWI WCCGGW 3 cut(s) 280, 397, 757
BsaXI ACNNNNNCTCC 4 cut(s) 481, 511, 771, 801
Bsc4I CCNNNNNNNGG 2 cut(s) 287, 485
Bse118I RCCGGY 1 cut(s) 280
Bse1I ACTGG 1 cut(s) 213
Bse8I GATNNNNATC 1 cut(s) 684
BseAI TCCGGA 1 cut(s) 757
BseDI CCNNGG 2 cut(s) 188, 309
BseGI GGATG 1 cut(s) 727
BseJI GATNNNNATC 1 cut(s) 684
BseLI CCNNNNNNNGG 2 cut(s) 287, 485
BseMII CTCAG 4 cut(s) 50, 86, 89, 764
BseNI ACTGG 1 cut(s) 213
BseRI GAGGAG 1 cut(s) 171
BseSI GKGCMC 1 cut(s) 919
BseXI GCAGC 3 cut(s) 180, 334, 933
BsgI GTGCAG 1 cut(s) 378
Bsh1236I CGCG 2 cut(s) 54, 799
Bsh1285I CGRYCG 1 cut(s) 612
BshFI GGCC 1 cut(s) 227
BshNI GGYRCC 1 cut(s) 445
BshTI ACCGGT 1 cut(s) 280
BsiEI CGRYCG 1 cut(s) 612
BsiHKAI GWGCWC 2 cut(s) 577, 919
BsiSI CCGG 4 cut(s) 281, 398, 692, 758
BslFI GGGAC 2 cut(s) 221, 611
BslI CCNNNNNNNGG 2 cut(s) 287, 485
BsmFI GGGAC 2 cut(s) 221, 611
BsnI GGCC 1 cut(s) 227
Bsp1286I GDGCHC 2 cut(s) 577, 919
Bsp13I TCCGGA 1 cut(s) 757
Bsp143I GATC 2 cut(s) 878, 951
BspACI CCGC 6 cut(s) 54, 148, 443, 566, 799, 908
BspANI GGCC 1 cut(s) 227
BspCNI CTCAG 4 cut(s) 49, 85, 90, 765
BspEI TCCGGA 1 cut(s) 757
BspFNI CGCG 2 cut(s) 54, 799
BspLI GGNNCC 6 cut(s) 228, 315, 387, 447, 522, 627
BspT107I GGYRCC 1 cut(s) 445
BsrFI RCCGGY 1 cut(s) 280
BsrI ACTGG 1 cut(s) 213
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 2 cut(s) 188, 309
BssMI GATC 2 cut(s) 878, 951
BssSI CACGAG 1 cut(s) 558
Bst2BI CACGAG 1 cut(s) 558
Bst4CI ACNGT 3 cut(s) 163, 745, 891
BstBAI YACGTR 1 cut(s) 204
BstC8I GCNNGC 3 cut(s) 78, 306, 919
BstDEI CTNAG 4 cut(s) 36, 72, 98, 773
BstF5I GGATG 1 cut(s) 727
BstFNI CGCG 2 cut(s) 54, 799
BstHHI GCGC 1 cut(s) 799
BstKTI GATC 2 cut(s) 881, 954
BstMBI GATC 2 cut(s) 878, 951
BstMCI CGRYCG 1 cut(s) 612
BstMWI GCNNNNNNNGC 5 cut(s) 452, 563, 572, 769, 914
BstNSI RCATGY 2 cut(s) 179, 638
BstSCI CCNGG 1 cut(s) 690
BstSFI CTRYAG 2 cut(s) 318, 828
BstSLI GKGCMC 1 cut(s) 919
BstUI CGCG 2 cut(s) 54, 799
BstV1I GCAGC 3 cut(s) 180, 334, 933
BstV2I GAAGAC 1 cut(s) 413
BstX2I RGATCY 1 cut(s) 951
BstYI RGATCY 1 cut(s) 951
BsuRI GGCC 1 cut(s) 227
BtsCI GGATG 1 cut(s) 727
BtsIMutI CAGTG 2 cut(s) 41, 95
Cac8I GCNNGC 3 cut(s) 78, 306, 919
CaiI CAGNNNCTG 1 cut(s) 43
CfoI GCGC 1 cut(s) 799
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 3 cut(s) 68, 226, 625
CseI GACGC 1 cut(s) 833
Csp6I GTAC 1 cut(s) 866
CspAI ACCGGT 1 cut(s) 280
CviAII CATG 2 cut(s) 176, 635
CviQI GTAC 1 cut(s) 866
DdeI CTNAG 4 cut(s) 36, 72, 98, 773
DpnI GATC 2 cut(s) 880, 953
DpnII GATC 2 cut(s) 878, 951
Ecl136II GAGCTC 1 cut(s) 575
Eco24I GRGCYC 1 cut(s) 577
Eco47I GGWCC 2 cut(s) 68, 625
Eco53kI GAGCTC 1 cut(s) 575
Eco57I CTGAAG 2 cut(s) 156, 354
EcoICRI GAGCTC 1 cut(s) 575
EcoO109I RGGNCCY 1 cut(s) 226
EcoRI GAATTC 1 cut(s) 737
EcoT38I GRGCYC 1 cut(s) 577
FaeI CATG 2 cut(s) 179, 638
FalI AAGNNNNNCTT 2 cut(s) 517, 549
FaqI GGGAC 2 cut(s) 221, 611
FatI CATG 2 cut(s) 175, 634
FauI CCCGC 2 cut(s) 141, 792
Fnu4HI GCNGC 8 cut(s) 6, 194, 348, 444, 567, 922, 944, 947
FokI GGATG 1 cut(s) 734
FriOI GRGCYC 1 cut(s) 577
Fsp4HI GCNGC 8 cut(s) 6, 194, 348, 444, 567, 922, 944, 947
FspBI CTAG 2 cut(s) 267, 855
GlaI GCGC 1 cut(s) 798
GluI GCNGC 8 cut(s) 6, 194, 348, 444, 567, 922, 944, 947
GsuI CTGGAG 1 cut(s) 196
HaeIII GGCC 1 cut(s) 227
HapII CCGG 4 cut(s) 281, 398, 692, 758
HgaI GACGC 1 cut(s) 833
HhaI GCGC 1 cut(s) 799
Hin1II CATG 2 cut(s) 179, 638
Hin6I GCGC 1 cut(s) 797
HinP1I GCGC 1 cut(s) 797
HinfI GANTC 4 cut(s) 57, 209, 356, 858
HpaII CCGG 4 cut(s) 281, 398, 692, 758
HphI GGTGA 4 cut(s) 270, 408, 415, 799
Hpy166II GTNNAC 1 cut(s) 917
Hpy188I TCNGA 4 cut(s) 189, 261, 361, 666
Hpy188III TCNNGA 5 cut(s) 243, 353, 560, 597, 758
Hpy8I GTNNAC 1 cut(s) 917
Hpy99I CGWCG 3 cut(s) 610, 821, 827
HpyAV CCTTC 3 cut(s) 639, 671, 722
HpyCH4III ACNGT 3 cut(s) 163, 745, 891
HpyCH4IV ACGT 3 cut(s) 203, 496, 713
HpyCH4V TGCA 3 cut(s) 395, 814, 917
HpyF10VI GCNNNNNNNGC 5 cut(s) 452, 563, 572, 769, 914
HpyF3I CTNAG 4 cut(s) 36, 72, 98, 773
HpySE526I ACGT 3 cut(s) 203, 496, 713
Hsp92II CATG 2 cut(s) 179, 638
HspAI GCGC 1 cut(s) 797
Kpn2I TCCGGA 1 cut(s) 757
Kzo9I GATC 2 cut(s) 878, 951
LmnI GCTCC 4 cut(s) 184, 262, 460, 760
Lsp1109I GCAGC 3 cut(s) 180, 334, 933
LweI GCATC 1 cut(s) 520
MaeI CTAG 2 cut(s) 267, 855
MaeII ACGT 3 cut(s) 203, 496, 713
MalI GATC 2 cut(s) 880, 953
MboI GATC 2 cut(s) 878, 951
MboII GAAGA 8 cut(s) 149, 244, 247, 418, 681, 943, 946, 949
MflI RGATCY 1 cut(s) 951
MhlI GDGCHC 2 cut(s) 577, 919
MluCI AATT 4 cut(s) 90, 466, 737, 843
MlyI GAGTC 3 cut(s) 66, 203, 852
MroI TCCGGA 1 cut(s) 757
MroXI GAANNNNTTC 1 cut(s) 891
MseI TTAA 2 cut(s) 156, 219
MslI CAYNNNNRTG 1 cut(s) 620
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 4 cut(s) 281, 398, 692, 758
MspR9I CCNGG 1 cut(s) 692
MvnI CGCG 2 cut(s) 54, 799
MwoI GCNNNNNNNGC 5 cut(s) 452, 563, 572, 769, 914
NciI CCSGG 1 cut(s) 692
NdeII GATC 2 cut(s) 878, 951
NlaIII CATG 2 cut(s) 179, 638
NlaIV GGNNCC 6 cut(s) 228, 315, 387, 447, 522, 627
NmeAIII GCCGAG 1 cut(s) 334
NspI RCATGY 2 cut(s) 179, 638
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 351, 710
PdmI GAANNNNTTC 1 cut(s) 891
PfeI GAWTC 1 cut(s) 356
PfoI TCCNGGA 1 cut(s) 690
PinAI ACCGGT 1 cut(s) 280
PkrI GCNGC 8 cut(s) 7, 195, 349, 445, 568, 923, 945, 948
PleI GAGTC 3 cut(s) 65, 203, 852
PpsI GAGTC 3 cut(s) 65, 203, 852
Ppu21I YACGTR 1 cut(s) 204
Psp124BI GAGCTC 1 cut(s) 577
PspN4I GGNNCC 6 cut(s) 228, 315, 387, 447, 522, 627
PspPI GGNCC 3 cut(s) 68, 226, 625
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 1 cut(s) 951
RsaI GTAC 1 cut(s) 867
RsaNI GTAC 1 cut(s) 866
RseI CAYNNNNRTG 1 cut(s) 620
SacI GAGCTC 1 cut(s) 577
SaqAI TTAA 2 cut(s) 156, 219
SatI GCNGC 8 cut(s) 6, 194, 348, 444, 567, 922, 944, 947
Sau3AI GATC 2 cut(s) 878, 951
Sau96I GGNCC 3 cut(s) 68, 226, 625
SchI GAGTC 3 cut(s) 66, 203, 852
ScrFI CCNGG 1 cut(s) 692
SduI GDGCHC 2 cut(s) 577, 919
SfaNI GCATC 1 cut(s) 520
SfcI CTRYAG 2 cut(s) 318, 828
SinI GGWCC 2 cut(s) 68, 625
SmiMI CAYNNNNRTG 1 cut(s) 620
SmlI CTYRAG 2 cut(s) 299, 926
SmoI CTYRAG 2 cut(s) 299, 926
Sse9I AATT 4 cut(s) 90, 466, 737, 843
SsiI CCGC 6 cut(s) 54, 148, 443, 566, 799, 908
SspMI CTAG 2 cut(s) 267, 855
SstI GAGCTC 1 cut(s) 577
StyD4I CCNGG 1 cut(s) 690
TaaI ACNGT 3 cut(s) 163, 745, 891
TaiI ACGT 3 cut(s) 206, 499, 716
TaqI TCGA 2 cut(s) 354, 704
TaqII GACCGA 1 cut(s) 598
TasI AATT 4 cut(s) 90, 466, 737, 843
TatI WGTACW 1 cut(s) 865
TauI GCSGC 2 cut(s) 446, 569
TfiI GAWTC 1 cut(s) 356
Tru1I TTAA 2 cut(s) 156, 219
Tru9I TTAA 2 cut(s) 156, 219
TscAI CASTG 2 cut(s) 48, 102
TseI GCWGC 6 cut(s) 5, 193, 347, 921, 943, 946
TspGWI ACGGA 2 cut(s) 81, 594
TspRI CASTG 2 cut(s) 48, 102
VneI GTGCAC 1 cut(s) 915
VpaK11BI GGWCC 2 cut(s) 68, 625
XapI RAATTY 2 cut(s) 90, 737
XceI RCATGY 2 cut(s) 179, 638
XcmI CCANNNNNNNNNTGG 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 891
XspI CTAG 2 cut(s) 267, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.