RLG00000011214

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005902
Physical Location & Seq
Reverse (-)
7727331 .. 7731623
4293 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011214

Sequence Viewer

Length: 1575 bp
ATGCGCAGCCGTCGTATTGGTGGAGGTGGCACTCCAGCTCCGGAGGCATGGGCGGCGGCGGTGGTGGCTCTGGTGCCAGAAACAACAAACTCAGCTTCGTCACTGCCCTCCATGTCTTCTGCAAGCTTTACCTCCACCGATCCACCAGCGGAGCTTAAATTCCGAATCTTAACACGGATCGTTCTGGATCCGAAGGTTTTCGAATCGAAGAAGGAGAAGAAGAGGTCGAGGTTGGTGATGAGTGATTCAGGGTCGAGTGATGAGCTATTGATGCCGAGGAGGAAAATTGGGCCGGAGACTATTAGGGTTTGTAATGGTTTGGACAAGGGCATTGTGGAAGGGGGCGGAATGGTTCCGAAGAGGAAGAGACTGGAGCATGGTATGCGGAATGAGGATTGTATGATTGGTGCAAATTATTTGGATGATGGTGGAGGAAGGAGAACTAATTTGGAGGTGTTTGAGTTCAATGAAGAAGACGGTGGGGCTCATGAGTCTACCAATACGCTGACAAGCAGAAGAGAGCAATACCAGGCCGCTTCTGGCCATCTGCCGATGCCTGTGGGTTCTTCAAGGTGGGAAACCTTCCATTCCCATAATCTGCCTGCGGTCAACTGCACGATTGAAAGTTGGGTTCTTATATCGTGGGCCTTAGTGGTGCCACAGAAACCTAGCGGAGTCTATGACGGACCTCAGCTCTCAAAACCTGAAATTTCACTGAGAAAGGAGGAAAACCCCCAGAACAAGAATGGTGCTGAAGAAAGAAAGCGGGTTATTAAAACAGTATGCTCGATACATGCTCCTCGTCCGAGGCTGCGATTTACGTGGGACTCCAGTATTAAAAAGGCTCCGAAGAAGAAGTCAAGAACACCATCAGCTCCGAAATCTAGGTCAAGCTCACTGGTTTCTGGGAGAAAGCTCAAGCATGCCGAGGAACCTATAGTTGCTTCTGCTGAAGTAAGGAACGCTGCTCTCGAATCCGATGTTGAGAAAAGCAGCAATAAGGAACCAACTGCACCGGAGGTGAAGACAACATCACCTCCACCTCCAAAAACGAAGTCAGCGGCACCATCAGCTCCAAAAATCAATTCAACACCACCTATGTCTGGGAGAAAACGTGGAAACGCAATAGTCAGGGAGGAACCAAGAGATGCTTCTATTGAAGCAAGGAGTGTTGCTCGTGAAGCGGCGAGAGCTCATCAAATGAGTTTAGCTTCTGGAACTCCGTCGGTCGTCATATCAATGGTCCCTTCGCATGTTTATAGAGTATTTTGGTTGAACCTTCCGAGAAGATTTTGTGATGGTAATCTCCCGGAAACTAAATCGAAACTGACGTTAGAGGATGAGGAAGGCAACGAATTCAAGGTTGTCTTTATTCCGGAGCGATATGGGCTGAGTGGAGGGTGGGTGAGCTTTGCGCGGGAAAAAAAGTTGCACGACGGCGACGCCGTAGTGCTTGAACAAATTGAAAAAACTAGACTCAAAGTACATATATTTAGATCAGCAGAACTGTTTCCCAATGTTCAAGCGGGGAGTGCACGCAGCCTTGAGAAGAAGAAGAAGCGGCAGCAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

525

Amino Acids

57.78

Weight (kDa)

9.81

Isoelectric Point (pI)

61.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 413 - 500 1.5e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AccB1I GGYRCC 3 cut(s) 73, 655, 1063
AccI GTMKAC 1 cut(s) 494
AccII CGCG 1 cut(s) 1417
AccIII TCCGGA 2 cut(s) 40, 1375
AclWI GGATC 4 cut(s) 134, 182, 185, 195
AcoI YGGCCR 1 cut(s) 541
AcsI RAATTY 3 cut(s) 158, 708, 1355
AcuI CTGAAG 2 cut(s) 774, 972
AcyI GRCGYC 1 cut(s) 1443
AfaI GTAC 1 cut(s) 1485
AfiI CCNNNNNNNGG 1 cut(s) 1103
AjnI CCWGG 1 cut(s) 528
AjuI GAANNNNNNNTTGG 4 cut(s) 1069, 1101, 1135, 1167
Alw21I GWGCWC 2 cut(s) 1195, 1537
Alw26I GTCTC 2 cut(s) 290, 361
Alw44I GTGCAC 1 cut(s) 1533
AlwI GGATC 4 cut(s) 134, 182, 185, 195
Aor13HI TCCGGA 2 cut(s) 40, 1375
AoxI GGCC 4 cut(s) 290, 531, 541, 645
ApaLI GTGCAC 1 cut(s) 1533
ApeKI GCWGC 6 cut(s) 6, 811, 965, 993, 1539, 1564
ApoI RAATTY 3 cut(s) 158, 708, 1355
ArsI GACNNNNNNTTYG 6 cut(s) 842, 872, 874, 904, 1040, 1072
Asp700I GAANNNNTTC 2 cut(s) 197, 1509
AspLEI GCGC 2 cut(s) 6, 1417
AspS9I GGNCC 4 cut(s) 290, 645, 686, 1243
AsuC2I CCSGG 1 cut(s) 1310
AsuHPI GGTGA 4 cut(s) 247, 1026, 1033, 1417
AsuII TTCGAA 1 cut(s) 201
AvaII GGWCC 2 cut(s) 686, 1243
BaeGI GKGCMC 1 cut(s) 1537
BalI TGGCCA 1 cut(s) 543
BamHI GGATCC 1 cut(s) 187
BanI GGYRCC 3 cut(s) 73, 655, 1063
BanII GRGCYC 2 cut(s) 487, 1195
BauI CACGAG 1 cut(s) 1176
BbsI GAAGAC 3 cut(s) 108, 480, 1031
Bbv12I GWGCWC 2 cut(s) 1195, 1537
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 5 cut(s) 18, 798, 952, 1005, 1551
BccI CCATC 5 cut(s) 419, 552, 877, 1075, 1292
BceAI ACGGC 2 cut(s) 1430, 1453
BciT130I CCWGG 1 cut(s) 530
BcnI CCSGG 1 cut(s) 1310
BcoDI GTCTC 2 cut(s) 290, 361
BfaI CTAG 3 cut(s) 669, 885, 1473
BfmI CTRYAG 1 cut(s) 936
BglII AGATCT 1 cut(s) 1569
Bme1390I CCNGG 2 cut(s) 530, 1310
Bme18I GGWCC 2 cut(s) 686, 1243
BmgT120I GGNCC 4 cut(s) 290, 645, 686, 1243
BmrFI CCNGG 2 cut(s) 530, 1310
BmsI GCATC 3 cut(s) 261, 543, 1138
BpiI GAAGAC 3 cut(s) 108, 480, 1031
BplI GAGNNNNNCTC 2 cut(s) 1159, 1191
BpmI CTGGAG 3 cut(s) 18, 392, 814
Bpu10I CCTNAGC 1 cut(s) 690
Bpu14I TTCGAA 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 902, 1565
BpuMI CCSGG 1 cut(s) 1310
BsaAI YACGTR 1 cut(s) 822
BsaBI GATNNNNATC 1 cut(s) 1302
BsaHI GRCGYC 1 cut(s) 1443
BsaJI CCNNGG 3 cut(s) 275, 806, 927
BsaWI WCCGGW 3 cut(s) 40, 1015, 1375
BsaXI ACNNNNNCTCC 8 cut(s) 22, 52, 1021, 1051, 1099, 1129, 1389, 1419
Bsc4I CCNNNNNNNGG 1 cut(s) 1103
Bse1I ACTGG 3 cut(s) 375, 831, 903
Bse8I GATNNNNATC 1 cut(s) 1302
BseAI TCCGGA 2 cut(s) 40, 1375
BseBI CCWGG 1 cut(s) 530
BseDI CCNNGG 3 cut(s) 275, 806, 927
BseGI GGATG 2 cut(s) 427, 1345
BseJI GATNNNNATC 1 cut(s) 1302
BseLI CCNNNNNNNGG 1 cut(s) 1103
BseMII CTCAG 4 cut(s) 105, 704, 707, 1382
BseNI ACTGG 3 cut(s) 375, 831, 903
BseRI GAGGAG 2 cut(s) 292, 789
BseSI GKGCMC 1 cut(s) 1537
BseXI GCAGC 5 cut(s) 18, 798, 952, 1005, 1551
BsgI GTGCAG 2 cut(s) 598, 996
Bsh1236I CGCG 1 cut(s) 1417
Bsh1285I CGRYCG 1 cut(s) 1230
BshFI GGCC 4 cut(s) 292, 533, 543, 647
BshNI GGYRCC 3 cut(s) 73, 655, 1063
BsiEI CGRYCG 1 cut(s) 1230
BsiHKAI GWGCWC 2 cut(s) 1195, 1537
BsiSI CCGG 5 cut(s) 41, 293, 1016, 1310, 1376
BslFI GGGAC 2 cut(s) 839, 1229
BslI CCNNNNNNNGG 1 cut(s) 1103
BsmAI GTCTC 2 cut(s) 290, 361
BsmFI GGGAC 2 cut(s) 839, 1229
BsnI GGCC 4 cut(s) 292, 533, 543, 647
Bsp119I TTCGAA 1 cut(s) 201
Bsp1286I GDGCHC 3 cut(s) 487, 1195, 1537
Bsp13I TCCGGA 2 cut(s) 40, 1375
Bsp143I GATC 5 cut(s) 139, 177, 187, 1496, 1569
BspANI GGCC 4 cut(s) 292, 533, 543, 647
BspCNI CTCAG 4 cut(s) 104, 703, 708, 1383
BspEI TCCGGA 2 cut(s) 40, 1375
BspFNI CGCG 1 cut(s) 1417
BspHI TCATGA 1 cut(s) 487
BspPI GGATC 4 cut(s) 134, 182, 185, 195
BspT104I TTCGAA 1 cut(s) 201
BspT107I GGYRCC 3 cut(s) 73, 655, 1063
BsrI ACTGG 3 cut(s) 375, 831, 903
BssECI CCNNGG 3 cut(s) 275, 806, 927
BssMI GATC 5 cut(s) 139, 177, 187, 1496, 1569
BssNI GRCGYC 1 cut(s) 1443
BssSI CACGAG 1 cut(s) 1176
Bst2BI CACGAG 1 cut(s) 1176
Bst2UI CCWGG 1 cut(s) 530
Bst4CI ACNGT 3 cut(s) 479, 781, 1509
Bst6I CTCTTC 4 cut(s) 215, 353, 359, 511
BstACI GRCGYC 1 cut(s) 1443
BstAPI GCANNNNNTGC 1 cut(s) 382
BstBAI YACGTR 1 cut(s) 822
BstBI TTCGAA 1 cut(s) 201
BstC8I GCNNGC 4 cut(s) 124, 603, 924, 1537
BstDEI CTNAG 5 cut(s) 91, 649, 690, 716, 1391
BstF5I GGATG 2 cut(s) 427, 1345
BstFNI CGCG 1 cut(s) 1417
BstHHI GCGC 2 cut(s) 6, 1417
BstKTI GATC 5 cut(s) 142, 180, 190, 1499, 1572
BstMAI GTCTC 2 cut(s) 290, 361
BstMBI GATC 5 cut(s) 139, 177, 187, 1496, 1569
BstMCI CGRYCG 1 cut(s) 1230
BstNI CCWGG 1 cut(s) 530
BstNSI RCATGY 3 cut(s) 797, 926, 1256
BstSCI CCNGG 2 cut(s) 528, 1308
BstSFI CTRYAG 1 cut(s) 936
BstSLI GKGCMC 1 cut(s) 1537
BstUI CGCG 1 cut(s) 1417
BstV1I GCAGC 5 cut(s) 18, 798, 952, 1005, 1551
BstV2I GAAGAC 3 cut(s) 108, 480, 1031
BstX2I RGATCY 2 cut(s) 187, 1569
BstYI RGATCY 2 cut(s) 187, 1569
BsuRI GGCC 4 cut(s) 292, 533, 543, 647
BtsCI GGATG 2 cut(s) 427, 1345
BtsI GCAGTG 1 cut(s) 101
BtsIMutI CAGTG 3 cut(s) 101, 713, 896
Cac8I GCNNGC 4 cut(s) 124, 603, 924, 1537
CciI TCATGA 1 cut(s) 487
CfoI GCGC 2 cut(s) 6, 1417
Cfr13I GGNCC 4 cut(s) 290, 645, 686, 1243
CseI GACGC 1 cut(s) 1451
Csp6I GTAC 1 cut(s) 1484
CviAII CATG 7 cut(s) 48, 112, 377, 488, 794, 923, 1253
CviQI GTAC 1 cut(s) 1484
DdeI CTNAG 5 cut(s) 91, 649, 690, 716, 1391
DpnI GATC 5 cut(s) 141, 179, 189, 1498, 1571
DpnII GATC 5 cut(s) 139, 177, 187, 1496, 1569
EaeI YGGCCR 1 cut(s) 541
Eam1104I CTCTTC 4 cut(s) 215, 353, 359, 511
EarI CTCTTC 4 cut(s) 215, 353, 359, 511
EciI GGCGGA 1 cut(s) 360
Ecl136II GAGCTC 1 cut(s) 1193
Eco24I GRGCYC 2 cut(s) 487, 1195
Eco47I GGWCC 2 cut(s) 686, 1243
Eco53kI GAGCTC 1 cut(s) 1193
Eco57I CTGAAG 2 cut(s) 774, 972
EcoICRI GAGCTC 1 cut(s) 1193
EcoRI GAATTC 1 cut(s) 1355
EcoRII CCWGG 1 cut(s) 528
EcoT38I GRGCYC 2 cut(s) 487, 1195
FaeI CATG 7 cut(s) 51, 115, 380, 491, 797, 926, 1256
FalI AAGNNNNNCTT 4 cut(s) 1135, 1167, 1352, 1384
FaqI GGGAC 2 cut(s) 839, 1229
FatI CATG 7 cut(s) 47, 111, 376, 487, 793, 922, 1252
FauI CCCGC 3 cut(s) 759, 1410, 1519
FblI GTMKAC 1 cut(s) 494
FokI GGATG 2 cut(s) 434, 1352
FriOI GRGCYC 2 cut(s) 487, 1195
FspBI CTAG 3 cut(s) 669, 885, 1473
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 2 cut(s) 5, 1416
GsuI CTGGAG 3 cut(s) 18, 392, 814
HaeIII GGCC 4 cut(s) 292, 533, 543, 647
HapII CCGG 5 cut(s) 41, 293, 1016, 1310, 1376
HgaI GACGC 1 cut(s) 1451
HhaI GCGC 2 cut(s) 6, 1417
Hin1I GRCGYC 1 cut(s) 1443
Hin1II CATG 7 cut(s) 51, 115, 380, 491, 797, 926, 1256
Hin6I GCGC 2 cut(s) 4, 1415
HinP1I GCGC 2 cut(s) 4, 1415
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HindIII AAGCTT 1 cut(s) 124
HinfI GANTC 8 cut(s) 165, 203, 245, 491, 675, 827, 974, 1476
HpaII CCGG 5 cut(s) 41, 293, 1016, 1310, 1376
HphI GGTGA 4 cut(s) 247, 1026, 1033, 1417
Hpy166II GTNNAC 3 cut(s) 495, 610, 1535
Hpy188I TCNGA 8 cut(s) 164, 192, 357, 807, 849, 879, 979, 1284
Hpy188III TCNNGA 8 cut(s) 41, 185, 488, 861, 971, 1178, 1215, 1376
Hpy8I GTNNAC 3 cut(s) 495, 610, 1535
Hpy99I CGWCG 4 cut(s) 15, 1228, 1439, 1445
HpyAV CCTTC 8 cut(s) 187, 205, 332, 429, 592, 1257, 1289, 1340
HpyCH4III ACNGT 3 cut(s) 479, 781, 1509
HpyCH4IV ACGT 3 cut(s) 821, 1114, 1331
HpyCH4V TGCA 6 cut(s) 122, 410, 615, 1013, 1432, 1535
HpyF3I CTNAG 5 cut(s) 91, 649, 690, 716, 1391
HpySE526I ACGT 3 cut(s) 821, 1114, 1331
Hsp92I GRCGYC 1 cut(s) 1443
Hsp92II CATG 7 cut(s) 51, 115, 380, 491, 797, 926, 1256
HspAI GCGC 2 cut(s) 4, 1415
Kpn2I TCCGGA 2 cut(s) 40, 1375
Kzo9I GATC 5 cut(s) 139, 177, 187, 1496, 1569
LmnI GCTCC 8 cut(s) 43, 151, 373, 802, 850, 880, 1078, 1378
Lsp1109I GCAGC 5 cut(s) 18, 798, 952, 1005, 1551
LweI GCATC 3 cut(s) 261, 543, 1138
MaeI CTAG 3 cut(s) 669, 885, 1473
MaeII ACGT 3 cut(s) 821, 1114, 1331
MaeIII GTNAC 1 cut(s) 99
MalI GATC 5 cut(s) 141, 179, 189, 1498, 1571
MboI GATC 5 cut(s) 139, 177, 187, 1496, 1569
MflI RGATCY 2 cut(s) 187, 1569
MhlI GDGCHC 3 cut(s) 487, 1195, 1537
MlsI TGGCCA 1 cut(s) 543
MluCI AATT 8 cut(s) 158, 285, 412, 445, 708, 1084, 1355, 1461
MluNI TGGCCA 1 cut(s) 543
MlyI GAGTC 4 cut(s) 500, 684, 821, 1470
Mox20I TGGCCA 1 cut(s) 543
MroI TCCGGA 2 cut(s) 40, 1375
MroXI GAANNNNTTC 2 cut(s) 197, 1509
MscI TGGCCA 1 cut(s) 543
MseI TTAA 4 cut(s) 156, 170, 774, 837
MslI CAYNNNNRTG 1 cut(s) 1238
Msp20I TGGCCA 1 cut(s) 543
MspA1I CMGCKG 2 cut(s) 149, 1061
MspI CCGG 5 cut(s) 41, 293, 1016, 1310, 1376
MspR9I CCNGG 2 cut(s) 530, 1310
MvaI CCWGG 1 cut(s) 530
MvnI CGCG 1 cut(s) 1417
NciI CCSGG 1 cut(s) 1310
NdeII GATC 5 cut(s) 139, 177, 187, 1496, 1569
NlaIII CATG 7 cut(s) 51, 115, 380, 491, 797, 926, 1256
NmeAIII GCCGAG 2 cut(s) 300, 952
NmuCI GTSAC 1 cut(s) 99
NsbI TGCGCA 1 cut(s) 5
NspI RCATGY 3 cut(s) 797, 926, 1256
NspV TTCGAA 1 cut(s) 201
PaeI GCATGC 1 cut(s) 926
PagI TCATGA 1 cut(s) 487
PcsI WCGNNNNNNNCGW 3 cut(s) 969, 1328, 1443
PdmI GAANNNNTTC 2 cut(s) 197, 1509
PfeI GAWTC 4 cut(s) 165, 203, 245, 974
PfoI TCCNGGA 1 cut(s) 1308
PleI GAGTC 4 cut(s) 499, 683, 821, 1470
PpsI GAGTC 4 cut(s) 499, 683, 821, 1470
Ppu21I YACGTR 1 cut(s) 822
Psp124BI GAGCTC 1 cut(s) 1195
Psp6I CCWGG 1 cut(s) 528
PspGI CCWGG 1 cut(s) 528
PspPI GGNCC 4 cut(s) 290, 645, 686, 1243
PsuI RGATCY 2 cut(s) 187, 1569
RsaI GTAC 1 cut(s) 1485
RsaNI GTAC 1 cut(s) 1484
RseI CAYNNNNRTG 1 cut(s) 1238
SacI GAGCTC 1 cut(s) 1195
SaqAI TTAA 4 cut(s) 156, 170, 774, 837
Sau3AI GATC 5 cut(s) 139, 177, 187, 1496, 1569
Sau96I GGNCC 4 cut(s) 290, 645, 686, 1243
SchI GAGTC 4 cut(s) 500, 684, 821, 1470
ScrFI CCNGG 2 cut(s) 530, 1310
SduI GDGCHC 3 cut(s) 487, 1195, 1537
SfaNI GCATC 3 cut(s) 261, 543, 1138
SfcI CTRYAG 1 cut(s) 936
SfuI TTCGAA 1 cut(s) 201
SinI GGWCC 2 cut(s) 686, 1243
SmiMI CAYNNNNRTG 1 cut(s) 1238
SmlI CTYRAG 2 cut(s) 917, 1544
SmoI CTYRAG 2 cut(s) 917, 1544
SphI GCATGC 1 cut(s) 926
Sse9I AATT 8 cut(s) 158, 285, 412, 445, 708, 1084, 1355, 1461
SspMI CTAG 3 cut(s) 669, 885, 1473
SstI GAGCTC 1 cut(s) 1195
StyD4I CCNGG 2 cut(s) 528, 1308
TaaI ACNGT 3 cut(s) 479, 781, 1509
TaiI ACGT 3 cut(s) 824, 1117, 1334
TaqI TCGA 7 cut(s) 201, 206, 227, 254, 788, 972, 1322
TaqII GACCGA 1 cut(s) 1216
TasI AATT 8 cut(s) 158, 285, 412, 445, 708, 1084, 1355, 1461
TatI WGTACW 1 cut(s) 1483
TauI GCSGC 6 cut(s) 56, 59, 536, 1064, 1187, 1564
TfiI GAWTC 4 cut(s) 165, 203, 245, 974
Tru1I TTAA 4 cut(s) 156, 170, 774, 837
Tru9I TTAA 4 cut(s) 156, 170, 774, 837
TscAI CASTG 3 cut(s) 108, 720, 903
TseFI GTSAC 1 cut(s) 99
TseI GCWGC 6 cut(s) 6, 811, 965, 993, 1539, 1564
Tsp45I GTSAC 1 cut(s) 99
TspDTI ATGAA 1 cut(s) 483
TspGWI ACGGA 3 cut(s) 190, 699, 1212
TspRI CASTG 3 cut(s) 108, 720, 903
VneI GTGCAC 1 cut(s) 1533
VpaK11BI GGWCC 2 cut(s) 686, 1243
XapI RAATTY 3 cut(s) 158, 708, 1355
XceI RCATGY 3 cut(s) 797, 926, 1256
XcmI CCANNNNNNNNNTGG 2 cut(s) 536, 743
XmiI GTMKAC 1 cut(s) 494
XmnI GAANNNNTTC 2 cut(s) 197, 1509
XspI CTAG 3 cut(s) 669, 885, 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.