Rh6AG422400

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0005902
Physical Location & Seq
Forward (+)
61559431 .. 61561820
2390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG422400.1

Sequence Viewer

Length: 960 bp
ATGGCTGCCACCTATGATAGCGAAGGAGTGAAATCTCAGCCACTGAAACCTAGAGGAGTACTCTATGACGGACCTCAGCTCTCAAAACCCGAAATTTCACTGAGAAAGGAGGAAAACCCCCAGAACAAGAATGGTGCTGAAGAAAGAAAGCGGGTTATTAAAACAGTATGCTCAATACATGCTCCTCGTCCGAGGCTGCGATTTACGTGGGACTCCAGTATTAAAAAGGCCCCGAAGAAGAAGTCAAGAACACCATCAGCTCCGAAATCTAGGTCAAGCTCACCGGTTTCTGGGAGAAAGCTCAAGCATGCCGAGGAACCTATAATTGCTTCTGCTGAAGTAAGGAACGCTGCTCTCGAATCCGATGTTGAGAAAAGCAGTAATAAGGAACCAACTGCACCGGAGGTGAAGACAACATCACCTCTACCTCCAAAAACGAAGTCAGCGGCACCATCAGCTCCAAAAATCAATTCAACACCACCTATGTCTGGGAGAAAACGTGGAAACGCAATAGTCAGGGAGGAACCAAGAGATGCTTCTATTGAAGCAAGGAGTATTGCTCGTGAAGCTGCGAGAGCTCATCAAATGAGTTTAGCTTCTGGAACTCCGTCGGTCGTCATATCAATGGTCCCTTCGCATGTTTATAGAGTATTTTGGTTGAACCTTCCGAGAAGATTTTGTGATGGTAATCTCCCGGAAACTAAATCGCAACTGACGTTAGAGGATGAGGAAGGCAACGAATTCACGGTTGTCTTTATTCCGGAGCGATATGGGCTGAGTGGAGGGTGGGTGAGCTTTGCGCGGGAAAAAAAGTTGCACGACGGCGACGCCGTAGTGCTTGAACAAATTGAAAAAACTAGACTCAAAGTACATATATTTAGATCAGCAGAACTGTTTCCCAATGTTCAAGCGGAGAGTGCACGCAGCCTTGAGAATAAGAAGAAGCAGCAGCAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

319

Amino Acids

35.4

Weight (kDa)

9.9

Isoelectric Point (pI)

61.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 208 - 295 1.5e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 448
AccII CGCG 1 cut(s) 802
AccIII TCCGGA 1 cut(s) 760
AciI CCGC 4 cut(s) 151, 446, 802, 911
AcsI RAATTY 2 cut(s) 93, 740
AcuI CTGAAG 2 cut(s) 159, 357
AcyI GRCGYC 1 cut(s) 828
AfaI GTAC 2 cut(s) 60, 870
AfiI CCNNNNNNNGG 2 cut(s) 290, 488
AgeI ACCGGT 1 cut(s) 283
AgsI TTSAA 6 cut(s) 474, 545, 661, 842, 851, 908
AjuI GAANNNNNNNTTGG 4 cut(s) 454, 486, 520, 552
AluBI AGCT 9 cut(s) 79, 260, 279, 301, 458, 569, 578, 596, 795
AluI AGCT 9 cut(s) 79, 260, 279, 301, 458, 569, 578, 596, 795
Alw21I GWGCWC 2 cut(s) 580, 922
Alw44I GTGCAC 1 cut(s) 918
AlwNI CAGNNNCTG 1 cut(s) 43
Aor13HI TCCGGA 1 cut(s) 760
AoxI GGCC 1 cut(s) 228
ApaLI GTGCAC 1 cut(s) 918
ApeKI GCWGC 7 cut(s) 5, 196, 350, 569, 924, 946, 949
ApoI RAATTY 2 cut(s) 93, 740
ArsI GACNNNNNNTTYG 6 cut(s) 227, 257, 259, 289, 425, 457
AsiGI ACCGGT 1 cut(s) 283
Asp700I GAANNNNTTC 1 cut(s) 894
AspLEI GCGC 1 cut(s) 802
AspS9I GGNCC 3 cut(s) 71, 229, 628
AsuC2I CCSGG 1 cut(s) 695
AsuHPI GGTGA 4 cut(s) 273, 411, 418, 802
AvaII GGWCC 2 cut(s) 71, 628
BaeGI GKGCMC 1 cut(s) 922
BanI GGYRCC 1 cut(s) 448
BanII GRGCYC 1 cut(s) 580
BauI CACGAG 1 cut(s) 561
BbsI GAAGAC 1 cut(s) 416
Bbv12I GWGCWC 2 cut(s) 580, 922
BbvCI CCTCAGC 1 cut(s) 75
BbvI GCAGC 4 cut(s) 183, 337, 556, 936
BccI CCATC 3 cut(s) 262, 460, 677
BceAI ACGGC 2 cut(s) 815, 838
BcnI CCSGG 1 cut(s) 695
BfaI CTAG 3 cut(s) 51, 270, 858
BglII AGATCT 1 cut(s) 954
BisI GCNGC 8 cut(s) 6, 197, 351, 447, 570, 925, 947, 950
BlsI GCNGC 8 cut(s) 7, 198, 352, 448, 571, 926, 948, 951
BmcAI AGTACT 1 cut(s) 60
Bme1390I CCNGG 1 cut(s) 695
Bme18I GGWCC 2 cut(s) 71, 628
BmgT120I GGNCC 3 cut(s) 71, 229, 628
BmiI GGNNCC 6 cut(s) 231, 318, 390, 450, 525, 630
BmrFI CCNGG 1 cut(s) 695
BmsI GCATC 1 cut(s) 523
BpiI GAAGAC 1 cut(s) 416
BplI GAGNNNNNCTC 4 cut(s) 45, 77, 544, 576
BpmI CTGGAG 1 cut(s) 199
Bpu10I CCTNAGC 1 cut(s) 75
BpuEI CTTGAG 2 cut(s) 287, 950
BpuMI CCSGG 1 cut(s) 695
BsaAI YACGTR 1 cut(s) 207
BsaBI GATNNNNATC 1 cut(s) 687
BsaHI GRCGYC 1 cut(s) 828
BsaJI CCNNGG 2 cut(s) 191, 312
BsaWI WCCGGW 3 cut(s) 283, 400, 760
BsaXI ACNNNNNCTCC 4 cut(s) 484, 514, 774, 804
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 488
Bse118I RCCGGY 1 cut(s) 283
Bse1I ACTGG 1 cut(s) 216
Bse8I GATNNNNATC 1 cut(s) 687
BseAI TCCGGA 1 cut(s) 760
BseDI CCNNGG 2 cut(s) 191, 312
BseGI GGATG 1 cut(s) 730
BseJI GATNNNNATC 1 cut(s) 687
BseLI CCNNNNNNNGG 2 cut(s) 290, 488
BseMII CTCAG 4 cut(s) 50, 89, 92, 767
BseNI ACTGG 1 cut(s) 216
BseRI GAGGAG 2 cut(s) 69, 174
BseSI GKGCMC 1 cut(s) 922
BseXI GCAGC 4 cut(s) 183, 337, 556, 936
BsgI GTGCAG 1 cut(s) 381
Bsh1236I CGCG 1 cut(s) 802
Bsh1285I CGRYCG 1 cut(s) 615
BshFI GGCC 1 cut(s) 230
BshNI GGYRCC 1 cut(s) 448
BshTI ACCGGT 1 cut(s) 283
BsiEI CGRYCG 1 cut(s) 615
BsiHKAI GWGCWC 2 cut(s) 580, 922
BsiSI CCGG 4 cut(s) 284, 401, 695, 761
BslFI GGGAC 2 cut(s) 224, 614
BslI CCNNNNNNNGG 2 cut(s) 290, 488
BsmFI GGGAC 2 cut(s) 224, 614
BsnI GGCC 1 cut(s) 230
Bsp1286I GDGCHC 2 cut(s) 580, 922
Bsp13I TCCGGA 1 cut(s) 760
Bsp143I GATC 2 cut(s) 881, 954
BspACI CCGC 4 cut(s) 151, 446, 802, 911
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 4 cut(s) 49, 88, 93, 768
BspEI TCCGGA 1 cut(s) 760
BspFNI CGCG 1 cut(s) 802
BspLI GGNNCC 6 cut(s) 231, 318, 390, 450, 525, 630
BspT107I GGYRCC 1 cut(s) 448
BsrFI RCCGGY 1 cut(s) 283
BsrI ACTGG 1 cut(s) 216
BssAI RCCGGY 1 cut(s) 283
BssECI CCNNGG 2 cut(s) 191, 312
BssMI GATC 2 cut(s) 881, 954
BssNI GRCGYC 1 cut(s) 828
BssSI CACGAG 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 561
Bst4CI ACNGT 3 cut(s) 166, 748, 894
BstACI GRCGYC 1 cut(s) 828
BstBAI YACGTR 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 309, 922
BstDEI CTNAG 4 cut(s) 36, 75, 101, 776
BstF5I GGATG 1 cut(s) 730
BstFNI CGCG 1 cut(s) 802
BstHHI GCGC 1 cut(s) 802
BstKTI GATC 2 cut(s) 884, 957
BstMBI GATC 2 cut(s) 881, 954
BstMCI CGRYCG 1 cut(s) 615
BstMWI GCNNNNNNNGC 5 cut(s) 455, 566, 575, 772, 917
BstNSI RCATGY 3 cut(s) 182, 311, 641
BstSCI CCNGG 1 cut(s) 693
BstSLI GKGCMC 1 cut(s) 922
BstUI CGCG 1 cut(s) 802
BstV1I GCAGC 4 cut(s) 183, 337, 556, 936
BstV2I GAAGAC 1 cut(s) 416
BstX2I RGATCY 1 cut(s) 954
BstYI RGATCY 1 cut(s) 954
BsuRI GGCC 1 cut(s) 230
BtsCI GGATG 1 cut(s) 730
BtsIMutI CAGTG 2 cut(s) 41, 98
Cac8I GCNNGC 2 cut(s) 309, 922
CaiI CAGNNNCTG 1 cut(s) 43
CfoI GCGC 1 cut(s) 802
Cfr10I RCCGGY 1 cut(s) 283
Cfr13I GGNCC 3 cut(s) 71, 229, 628
CseI GACGC 1 cut(s) 836
Csp6I GTAC 2 cut(s) 59, 869
CspAI ACCGGT 1 cut(s) 283
CviAII CATG 3 cut(s) 179, 308, 638
CviQI GTAC 2 cut(s) 59, 869
DdeI CTNAG 4 cut(s) 36, 75, 101, 776
DpnI GATC 2 cut(s) 883, 956
DpnII GATC 2 cut(s) 881, 954
Ecl136II GAGCTC 1 cut(s) 578
Eco24I GRGCYC 1 cut(s) 580
Eco47I GGWCC 2 cut(s) 71, 628
Eco53kI GAGCTC 1 cut(s) 578
Eco57I CTGAAG 2 cut(s) 159, 357
EcoICRI GAGCTC 1 cut(s) 578
EcoO109I RGGNCCY 1 cut(s) 229
EcoRI GAATTC 1 cut(s) 740
EcoT38I GRGCYC 1 cut(s) 580
FaeI CATG 3 cut(s) 182, 311, 641
FalI AAGNNNNNCTT 2 cut(s) 520, 552
FaqI GGGAC 2 cut(s) 224, 614
FatI CATG 3 cut(s) 178, 307, 637
FauI CCCGC 2 cut(s) 144, 795
Fnu4HI GCNGC 8 cut(s) 6, 197, 351, 447, 570, 925, 947, 950
FokI GGATG 1 cut(s) 737
FriOI GRGCYC 1 cut(s) 580
Fsp4HI GCNGC 8 cut(s) 6, 197, 351, 447, 570, 925, 947, 950
FspBI CTAG 3 cut(s) 51, 270, 858
GlaI GCGC 1 cut(s) 801
GluI GCNGC 8 cut(s) 6, 197, 351, 447, 570, 925, 947, 950
GsuI CTGGAG 1 cut(s) 199
HaeIII GGCC 1 cut(s) 230
HapII CCGG 4 cut(s) 284, 401, 695, 761
HgaI GACGC 1 cut(s) 836
HhaI GCGC 1 cut(s) 802
Hin1I GRCGYC 1 cut(s) 828
Hin1II CATG 3 cut(s) 182, 311, 641
Hin6I GCGC 1 cut(s) 800
HinP1I GCGC 1 cut(s) 800
HinfI GANTC 3 cut(s) 212, 359, 861
HpaII CCGG 4 cut(s) 284, 401, 695, 761
HphI GGTGA 4 cut(s) 273, 411, 418, 802
Hpy166II GTNNAC 1 cut(s) 920
Hpy188I TCNGA 4 cut(s) 192, 264, 364, 669
Hpy188III TCNNGA 5 cut(s) 246, 356, 563, 600, 761
Hpy8I GTNNAC 1 cut(s) 920
Hpy99I CGWCG 3 cut(s) 613, 824, 830
HpyAV CCTTC 4 cut(s) 17, 642, 674, 725
HpyCH4III ACNGT 3 cut(s) 166, 748, 894
HpyCH4IV ACGT 3 cut(s) 206, 499, 716
HpyCH4V TGCA 3 cut(s) 398, 817, 920
HpyF10VI GCNNNNNNNGC 5 cut(s) 455, 566, 575, 772, 917
HpyF3I CTNAG 4 cut(s) 36, 75, 101, 776
HpySE526I ACGT 3 cut(s) 206, 499, 716
Hsp92I GRCGYC 1 cut(s) 828
Hsp92II CATG 3 cut(s) 182, 311, 641
HspAI GCGC 1 cut(s) 800
Kpn2I TCCGGA 1 cut(s) 760
Kzo9I GATC 2 cut(s) 881, 954
LmnI GCTCC 4 cut(s) 187, 265, 463, 763
Lsp1109I GCAGC 4 cut(s) 183, 337, 556, 936
LweI GCATC 1 cut(s) 523
MaeI CTAG 3 cut(s) 51, 270, 858
MaeII ACGT 3 cut(s) 206, 499, 716
MalI GATC 2 cut(s) 883, 956
MboI GATC 2 cut(s) 881, 954
MboII GAAGA 6 cut(s) 152, 247, 250, 421, 684, 952
MflI RGATCY 1 cut(s) 954
MhlI GDGCHC 2 cut(s) 580, 922
MluCI AATT 5 cut(s) 93, 324, 469, 740, 846
MlyI GAGTC 2 cut(s) 206, 855
MroI TCCGGA 1 cut(s) 760
MroXI GAANNNNTTC 1 cut(s) 894
MseI TTAA 2 cut(s) 159, 222
MslI CAYNNNNRTG 1 cut(s) 623
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 4 cut(s) 284, 401, 695, 761
MspR9I CCNGG 1 cut(s) 695
MvnI CGCG 1 cut(s) 802
MwoI GCNNNNNNNGC 5 cut(s) 455, 566, 575, 772, 917
NciI CCSGG 1 cut(s) 695
NdeII GATC 2 cut(s) 881, 954
NlaIII CATG 3 cut(s) 182, 311, 641
NlaIV GGNNCC 6 cut(s) 231, 318, 390, 450, 525, 630
NmeAIII GCCGAG 1 cut(s) 337
NspI RCATGY 3 cut(s) 182, 311, 641
PaeI GCATGC 1 cut(s) 311
PcsI WCGNNNNNNNCGW 3 cut(s) 354, 713, 828
PdmI GAANNNNTTC 1 cut(s) 894
PfeI GAWTC 1 cut(s) 359
PfoI TCCNGGA 1 cut(s) 693
PinAI ACCGGT 1 cut(s) 283
PkrI GCNGC 8 cut(s) 7, 198, 352, 448, 571, 926, 948, 951
PleI GAGTC 2 cut(s) 206, 855
PpsI GAGTC 2 cut(s) 206, 855
Ppu21I YACGTR 1 cut(s) 207
Psp124BI GAGCTC 1 cut(s) 580
PspN4I GGNNCC 6 cut(s) 231, 318, 390, 450, 525, 630
PspPI GGNCC 3 cut(s) 71, 229, 628
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 1 cut(s) 954
RsaI GTAC 2 cut(s) 60, 870
RsaNI GTAC 2 cut(s) 59, 869
RseI CAYNNNNRTG 1 cut(s) 623
SacI GAGCTC 1 cut(s) 580
SaqAI TTAA 2 cut(s) 159, 222
SatI GCNGC 8 cut(s) 6, 197, 351, 447, 570, 925, 947, 950
Sau3AI GATC 2 cut(s) 881, 954
Sau96I GGNCC 3 cut(s) 71, 229, 628
ScaI AGTACT 1 cut(s) 60
SchI GAGTC 2 cut(s) 206, 855
ScrFI CCNGG 1 cut(s) 695
SduI GDGCHC 2 cut(s) 580, 922
SfaNI GCATC 1 cut(s) 523
SinI GGWCC 2 cut(s) 71, 628
SmiMI CAYNNNNRTG 1 cut(s) 623
SmlI CTYRAG 2 cut(s) 302, 929
SmoI CTYRAG 2 cut(s) 302, 929
SphI GCATGC 1 cut(s) 311
Sse9I AATT 5 cut(s) 93, 324, 469, 740, 846
SsiI CCGC 4 cut(s) 151, 446, 802, 911
SspMI CTAG 3 cut(s) 51, 270, 858
SstI GAGCTC 1 cut(s) 580
StyD4I CCNGG 1 cut(s) 693
TaaI ACNGT 3 cut(s) 166, 748, 894
TaiI ACGT 3 cut(s) 209, 502, 719
TaqI TCGA 1 cut(s) 357
TaqII GACCGA 1 cut(s) 601
TasI AATT 5 cut(s) 93, 324, 469, 740, 846
TatI WGTACW 2 cut(s) 58, 868
TauI GCSGC 1 cut(s) 449
TfiI GAWTC 1 cut(s) 359
Tru1I TTAA 2 cut(s) 159, 222
Tru9I TTAA 2 cut(s) 159, 222
TscAI CASTG 2 cut(s) 48, 105
TseI GCWGC 7 cut(s) 5, 196, 350, 569, 924, 946, 949
TspGWI ACGGA 2 cut(s) 84, 597
TspRI CASTG 2 cut(s) 48, 105
VneI GTGCAC 1 cut(s) 918
VpaK11BI GGWCC 2 cut(s) 71, 628
XapI RAATTY 2 cut(s) 93, 740
XceI RCATGY 3 cut(s) 182, 311, 641
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XmnI GAANNNNTTC 1 cut(s) 894
XspI CTAG 3 cut(s) 51, 270, 858
ZrmI AGTACT 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.