FvH4_6g11810

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005902
Physical Location & Seq
Reverse (-)
7052976 .. 7054036
1061 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g11810.t1

Sequence Viewer

Length: 774 bp
ATGAAGAAGAAGGCCAGAGAGATCGCGATCGGAAGTCTGTGCAGCAACCTCACTCAGAACTTCACGACTGATCATGGTGGACCTCCGATCGCGATCTCTCCGGCCTTCTTCTTCATTCCAACACCGTCGGGGGAGGTCTGGGATCACGCTGGGGAGAAGAAACGGTGTCGCCGGCGCTGGAATGGTGGAGAAGCGGCGGAAATCCGGACGGAAAAGCCGTCCGGACCTGATAATCCCTGCTGTTTTGCGCAGGAAGTCAATACATTGGCCCCGATTGCAAAAAAGCAGGCTCGGGCAAACACAGGATCAAACTCAAAGGAGGTTATCCTATACAATGTTCCGGATGAACTTCGTAACGTCAAGGAATATGCCTACTGTCATCGTGTTCGAACCAAAGATCCTGAAAAGCCTGACAGACTCAAGATCACAGAATACGTCGATGTTGTTCATGTTCTAGCAACAAAATTGAAGATTCGTAGAGGCTGTAGTGGAGAAAGTGCTCAAGATCAGGTCATCAGTAATATTACAGAGTTCATGTCACCTGATGGTTGCTTCATACTGGAACTTTTCCTCAGGTTTGACCAATACTACTTGAATAATAAGAAGCTAGAAGATCAATCTGATAATGGAATCTTCAAATATGCTTGGATGATCCCAGCACTTCGGCGTGATCTTGCTTTACTTGAAAATCAGATCCCATTCTTTGTTCTAGAAGAATTGTATGACTTCATCAAGCCCCATATCAAGGGTAACGCACCAAACTCAGGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

258

Amino Acids

29.19

Weight (kDa)

8.39

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 124 - 247 1.2e-19 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 249
AccII CGCG 2 cut(s) 26, 92
AccIII TCCGGA 3 cut(s) 204, 221, 340
AciI CCGC 2 cut(s) 194, 197
AclWI GGATC 5 cut(s) 150, 313, 392, 646, 688
AfiI CCNNNNNNNGG 2 cut(s) 745, 764
AgsI TTSAA 4 cut(s) 469, 595, 637, 686
AloI GAACNNNNNNTCC 2 cut(s) 382, 414
AluBI AGCT 1 cut(s) 607
AluI AGCT 1 cut(s) 607
Alw21I GWGCWC 1 cut(s) 502
AlwI GGATC 5 cut(s) 150, 313, 392, 646, 688
Ama87I CYCGRG 1 cut(s) 291
Aor13HI TCCGGA 3 cut(s) 204, 221, 340
AoxI GGCC 3 cut(s) 12, 102, 267
ApeKI GCWGC 1 cut(s) 42
AspLEI GCGC 2 cut(s) 177, 250
AspS9I GGNCC 3 cut(s) 80, 224, 268
AsuHPI GGTGA 1 cut(s) 531
AsuII TTCGAA 1 cut(s) 388
AvaI CYCGRG 1 cut(s) 291
AvaII GGWCC 2 cut(s) 80, 224
AxyI CCTNAGG 1 cut(s) 572
Bbv12I GWGCWC 1 cut(s) 502
BbvI GCAGC 1 cut(s) 54
BccI CCATC 1 cut(s) 539
BceAI ACGGC 1 cut(s) 202
BclI TGATCA 1 cut(s) 70
BfaI CTAG 4 cut(s) 455, 608, 710, 772
BfmI CTRYAG 1 cut(s) 484
BfoI RGCGCY 1 cut(s) 178
BisI GCNGC 2 cut(s) 43, 195
BlsI GCNGC 2 cut(s) 44, 196
Bme18I GGWCC 2 cut(s) 80, 224
BmeT110I CYCGRG 1 cut(s) 291
BmgT120I GGNCC 3 cut(s) 80, 224, 268
BmiI GGNNCC 1 cut(s) 270
Bpu14I TTCGAA 1 cut(s) 388
BpuEI CTTGAG 2 cut(s) 404, 486
BsaBI GATNNNNATC 2 cut(s) 26, 92
BsaWI WCCGGW 3 cut(s) 204, 221, 340
Bsc4I CCNNNNNNNGG 2 cut(s) 745, 764
Bse118I RCCGGY 1 cut(s) 171
Bse1I ACTGG 1 cut(s) 564
Bse21I CCTNAGG 1 cut(s) 572
Bse8I GATNNNNATC 2 cut(s) 26, 92
BseAI TCCGGA 3 cut(s) 204, 221, 340
BseGI GGATG 2 cut(s) 349, 654
BseJI GATNNNNATC 2 cut(s) 26, 92
BseLI CCNNNNNNNGG 2 cut(s) 745, 764
BseMII CTCAG 2 cut(s) 68, 586
BseNI ACTGG 1 cut(s) 564
BseXI GCAGC 1 cut(s) 54
BseYI CCCAGC 2 cut(s) 149, 655
BsgI GTGCAG 1 cut(s) 61
Bsh1236I CGCG 2 cut(s) 26, 92
Bsh1285I CGRYCG 2 cut(s) 30, 90
BshFI GGCC 3 cut(s) 14, 104, 269
BsiEI CGRYCG 2 cut(s) 30, 90
BsiHKAI GWGCWC 1 cut(s) 502
BsiHKCI CYCGRG 1 cut(s) 291
BsiSI CCGG 5 cut(s) 101, 172, 205, 222, 341
BslI CCNNNNNNNGG 2 cut(s) 745, 764
BsnI GGCC 3 cut(s) 14, 104, 269
BsoBI CYCGRG 1 cut(s) 291
Bsp119I TTCGAA 1 cut(s) 388
Bsp1286I GDGCHC 1 cut(s) 502
Bsp13I TCCGGA 3 cut(s) 204, 221, 340
Bsp68I TCGCGA 2 cut(s) 26, 92
BspACI CCGC 2 cut(s) 194, 197
BspANI GGCC 3 cut(s) 14, 104, 269
BspCNI CTCAG 2 cut(s) 67, 585
BspEI TCCGGA 3 cut(s) 204, 221, 340
BspFNI CGCG 2 cut(s) 26, 92
BspLI GGNNCC 1 cut(s) 270
BspPI GGATC 5 cut(s) 150, 313, 392, 646, 688
BspT104I TTCGAA 1 cut(s) 388
BsrFI RCCGGY 1 cut(s) 171
BsrI ACTGG 1 cut(s) 564
BssAI RCCGGY 1 cut(s) 171
Bst4CI ACNGT 3 cut(s) 126, 165, 377
BstBI TTCGAA 1 cut(s) 388
BstC8I GCNNGC 2 cut(s) 173, 288
BstDEI CTNAG 3 cut(s) 54, 572, 763
BstF5I GGATG 2 cut(s) 349, 654
BstFNI CGCG 2 cut(s) 26, 92
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 2 cut(s) 177, 250
BstMCI CGRYCG 2 cut(s) 30, 90
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstSFI CTRYAG 1 cut(s) 484
BstUI CGCG 2 cut(s) 26, 92
BstV1I GCAGC 1 cut(s) 54
BstX2I RGATCY 2 cut(s) 397, 693
BstYI RGATCY 2 cut(s) 397, 693
Bsu36I CCTNAGG 1 cut(s) 572
BsuRI GGCC 3 cut(s) 14, 104, 269
BtsCI GGATG 2 cut(s) 349, 654
BtuMI TCGCGA 2 cut(s) 26, 92
Cac8I GCNNGC 2 cut(s) 173, 288
CfoI GCGC 2 cut(s) 177, 250
Cfr10I RCCGGY 1 cut(s) 171
Cfr13I GGNCC 3 cut(s) 80, 224, 268
CviAII CATG 3 cut(s) 74, 449, 535
CviJI RGCY 9 cut(s) 14, 104, 217, 269, 290, 409, 483, 607, 736
CviKI_1 RGCY 9 cut(s) 14, 104, 217, 269, 290, 409, 483, 607, 736
DdeI CTNAG 3 cut(s) 54, 572, 763
EciI GGCGGA 1 cut(s) 212
Eco47I GGWCC 2 cut(s) 80, 224
Eco81I CCTNAGG 1 cut(s) 572
Eco88I CYCGRG 1 cut(s) 291
FaeI CATG 3 cut(s) 77, 452, 538
FaiI YATR 9 cut(s) 75, 331, 369, 450, 536, 557, 642, 723, 741
FatI CATG 3 cut(s) 73, 448, 534
FbaI TGATCA 1 cut(s) 70
Fnu4HI GCNGC 2 cut(s) 43, 195
FokI GGATG 2 cut(s) 356, 661
Fsp4HI GCNGC 2 cut(s) 43, 195
FspBI CTAG 4 cut(s) 455, 608, 710, 772
FspI TGCGCA 1 cut(s) 249
GlaI GCGC 2 cut(s) 176, 249
GluI GCNGC 2 cut(s) 43, 195
GsaI CCCAGC 2 cut(s) 153, 659
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 3 cut(s) 14, 104, 269
HapII CCGG 5 cut(s) 101, 172, 205, 222, 341
HhaI GCGC 2 cut(s) 177, 250
Hin1II CATG 3 cut(s) 77, 452, 538
Hin6I GCGC 2 cut(s) 175, 248
HinP1I GCGC 2 cut(s) 175, 248
HinfI GANTC 3 cut(s) 417, 472, 630
HpaII CCGG 5 cut(s) 101, 172, 205, 222, 341
HphI GGTGA 1 cut(s) 531
Hpy166II GTNNAC 1 cut(s) 80
Hpy188I TCNGA 5 cut(s) 32, 57, 87, 622, 693
Hpy8I GTNNAC 1 cut(s) 80
Hpy99I CGWCG 2 cut(s) 130, 440
HpyAV CCTTC 2 cut(s) 4, 115
HpyCH4III ACNGT 3 cut(s) 126, 165, 377
HpyCH4IV ACGT 2 cut(s) 357, 435
HpyCH4V TGCA 2 cut(s) 42, 278
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpyF3I CTNAG 3 cut(s) 54, 572, 763
HpySE526I ACGT 2 cut(s) 357, 435
Hsp92II CATG 3 cut(s) 77, 452, 538
HspAI GCGC 2 cut(s) 175, 248
Kpn2I TCCGGA 3 cut(s) 204, 221, 340
KroI GCCGGC 1 cut(s) 171
KroNI GCCGGC 1 cut(s) 173
Ksp22I TGATCA 1 cut(s) 70
Lsp1109I GCAGC 1 cut(s) 54
MaeI CTAG 4 cut(s) 455, 608, 710, 772
MaeII ACGT 2 cut(s) 357, 435
MaeIII GTNAC 4 cut(s) 353, 537, 749, 767
MboII GAAGA 9 cut(s) 16, 19, 100, 103, 169, 481, 623, 625, 725
MflI RGATCY 2 cut(s) 397, 693
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 2 cut(s) 464, 716
MlyI GAGTC 1 cut(s) 411
MmeI TCCRAC 1 cut(s) 143
MnlI CCTC 6 cut(s) 59, 93, 127, 313, 473, 581
MreI CGCCGGCG 1 cut(s) 171
MroI TCCGGA 3 cut(s) 204, 221, 340
MroNI GCCGGC 1 cut(s) 171
MspI CCGG 5 cut(s) 101, 172, 205, 222, 341
MvnI CGCG 2 cut(s) 26, 92
MwoI GCNNNNNNNGC 1 cut(s) 275
NaeI GCCGGC 1 cut(s) 173
NgoMIV GCCGGC 1 cut(s) 171
NlaIII CATG 3 cut(s) 77, 452, 538
NlaIV GGNNCC 1 cut(s) 270
NmuCI GTSAC 2 cut(s) 537, 767
NruI TCGCGA 2 cut(s) 26, 92
NsbI TGCGCA 1 cut(s) 249
NspV TTCGAA 1 cut(s) 388
PcsI WCGNNNNNNNCGW 1 cut(s) 215
PdiI GCCGGC 1 cut(s) 173
PfeI GAWTC 2 cut(s) 472, 630
PkrI GCNGC 2 cut(s) 44, 196
Ple19I CGATCG 2 cut(s) 30, 90
PleI GAGTC 1 cut(s) 411
PpsI GAGTC 1 cut(s) 411
PspFI CCCAGC 2 cut(s) 149, 655
PspN4I GGNNCC 1 cut(s) 270
PspPI GGNCC 3 cut(s) 80, 224, 268
PsuI RGATCY 2 cut(s) 397, 693
PvuI CGATCG 2 cut(s) 30, 90
RruI TCGCGA 2 cut(s) 26, 92
SatI GCNGC 2 cut(s) 43, 195
Sau96I GGNCC 3 cut(s) 80, 224, 268
SchI GAGTC 1 cut(s) 411
SduI GDGCHC 1 cut(s) 502
SfcI CTRYAG 1 cut(s) 484
SfuI TTCGAA 1 cut(s) 388
SgrAI CRCCGGYG 1 cut(s) 171
SinI GGWCC 2 cut(s) 80, 224
SmlI CTYRAG 2 cut(s) 419, 501
SmoI CTYRAG 2 cut(s) 419, 501
Sse9I AATT 2 cut(s) 464, 716
SsiI CCGC 2 cut(s) 194, 197
SspI AATATT 1 cut(s) 523
SspMI CTAG 4 cut(s) 455, 608, 710, 772
TaaI ACNGT 3 cut(s) 126, 165, 377
TaiI ACGT 2 cut(s) 360, 438
TaqI TCGA 2 cut(s) 388, 438
TasI AATT 2 cut(s) 464, 716
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 2 cut(s) 472, 630
TseFI GTSAC 2 cut(s) 537, 767
TseI GCWGC 1 cut(s) 42
Tsp45I GTSAC 2 cut(s) 537, 767
TspDTI ATGAA 7 cut(s) 17, 103, 360, 437, 523, 544, 718
TspGWI ACGGA 1 cut(s) 224
VpaK11BI GGWCC 2 cut(s) 80, 224
XbaI TCTAGA 1 cut(s) 709
XspI CTAG 4 cut(s) 455, 608, 710, 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.