FvH4_3g01320

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008844
Physical Location & Seq
Forward (+)
670002 .. 673771
3770 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g01320.t1

Sequence Viewer

Length: 1875 bp
ATGATGAAAGACAGAGATATGCTTATGAATAGCAGTGTTGAGTTTGGTGAGCGTATTTACGAGCTTTTCTCAGAAGGGAACTGGAGTGAAATACAGAGCTTTTACGAGGAACAGAGTTTTCAGGTTTGCATACCTCTTAATCATTTGGGAGATACGGCTCTTCATCTTGCAGCATACAGTCAAGACATGAAATTGGTGGAATCCCTGCTGAACATAGTGTATGTTGAAGACAAACAATTTTCATCGACTCCAGTAGATGAACAAGATGGCTTGAAACTCTCCTTAAAGAATAAATTTGGAAACACACCACTCCATGAGGCAGCTTCGACAGGAAATGTAAAATTGGTAGAGCTGATGTTGGGTTTTGAAGAGAAGAGGCGACTGATGACGAATACTAGAAACAAGGACGATCAGACACCTTTCTTTATGGCTGCAATTTATGGCAAGACCGAGTTGATCAAAACTTGGGTGGAAAAGCTTAACAACATAGAAAGACATGTCAAGACTGAACACTCCACCATGCTTCATGAGGCTATTCGCGGGTATTACTTTGACACAGCTCTGGAATTATTAGACAAAAAATACTGCATACCGAAGGATCAGAAAGATTTATATGGCATGACCTGCTTTCAGCTGCTAGCTGACATTCCCAACGCTTTCAAGAGTGGATACTCCTTTGGCTTCTTGGAGAAACTGATTTATGACGGCCTTCCAGTTCCAAAAGATATGGATGCAAGTGTAAACAACGATGTATCTCGGGGCAATCCAGACACACATCATGGATTCCTAAGAGTCCAATGTCTAAGAAACATGAAGAAGAATCATGGCAGAGTGATGAAGCTTACCAAAACTCTGGCTGAAGAAGACAAGTCATGGCAGGGTCATAATACTACTGATGGTCAGAGTGATACTGCAGCTGCAAAGAATGACACTCCACTTATTGCAGCTGCAAAGAATGGGATCGTAGAGATTGTTGAGGCCATCTTAGATGTGTTCCCACAGGCCATCGAGCATGAAAACCACAAAGGAGAAAATGTGTTTCATGTTGCTGTGAAGAATCGCAGGAAAAATGTTTTGGTTCTTCTCGAAGATAAGAAATTTAATTATCCGATCGCGAGGCACGTTCGGAAGTTCGATAAAGCTGATAATGGTATTATACATAAAGCTGCATACAAGACTGATATTTCCTCAAGGGAGAGGCCCGGGGAAGCCCTTTGCTTGCAGTCAGATATTCAGTGGTTTGAGAGAGTGAGGAAAATGGTGCCTGCACATCATATCAACCAGAAAAATAAAAAAGGAGATACGCCTCAAGCTTTGTTCACAAAACAGCATGAAGAACTGGTGAAGGAAGGCCGAGAGTGGTTGATCAGAACAACAAACTCATGCATTGTAGTTGCAGTTCTTATTGCAACTGTTGCTTTTACAAGTATCTACACCGTGCCTGGCGGTTCTGATAAGAATGGCAAGCCGTTGCTCCTCAACACAACTGCATTCACGATCTTCACAGCATCAGATGTGGCAGCTCTTTGTTTTGCTTTCACCTCAGTCGTGGTGTTTCTATCCATTACTACGTCCAAAATGAATGAGCAAGATTTCAGGATATCTCTGCCTCTGAAGCTTGTTTTGGGGCTAAGCATGCTGTTTTTTTCTGTGACAGCATTGATGATTGGTTTTGCCGCTACTCTTGTCATTACAATAAGGCACAGGGTACATCAGGCTGCTGCTCCCATCATTGCCATTGCTTGTTTTCCTGTCGCCTTTTTCCTCCTTCTCCAGCTTCCTCTCTATACCAATATTACTTGGTTTACCTTAAAGGACTTGGTGCGCAGCCTCATTCATCTTCTTCCTCTGAAAAAATGTCAGCCTCGACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

625

Amino Acids

70.71

Weight (kDa)

8.36

Isoelectric Point (pI)

32.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 47 - 120 3.5e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 304 - 364 1.4e-06 Ankyrin repeats (3 copies)
PGG PF13962 453 - 564 2.4e-22 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1826
Acc36I ACCTGC 1 cut(s) 632
AccB1I GGYRCC 1 cut(s) 1261
AccII CGCG 2 cut(s) 540, 1115
AciI CCGC 3 cut(s) 540, 1446, 1677
AclWI GGATC 2 cut(s) 606, 968
AcsI RAATTY 2 cut(s) 293, 1097
AcuI CTGAAG 2 cut(s) 879, 1634
AfaI GTAC 1 cut(s) 1710
AflIII ACRYGT 1 cut(s) 496
AgsI TTSAA 4 cut(s) 227, 274, 368, 661
AjnI CCWGG 1 cut(s) 1441
AjuI GAANNNNNNNTTGG 4 cut(s) 1058, 1090, 1607, 1639
AlwI GGATC 2 cut(s) 606, 968
Ama87I CYCGRG 2 cut(s) 756, 1202
AoxI GGCC 5 cut(s) 706, 978, 1002, 1199, 1351
ApoI RAATTY 2 cut(s) 293, 1097
AspLEI GCGC 1 cut(s) 1827
AspS9I GGNCC 1 cut(s) 1200
AsuC2I CCSGG 2 cut(s) 1203, 1204
AsuHPI GGTGA 3 cut(s) 59, 1354, 1531
AsuNHI GCTAGC 1 cut(s) 637
AvaI CYCGRG 2 cut(s) 756, 1202
BanI GGYRCC 1 cut(s) 1261
BbsI GAAGAC 2 cut(s) 234, 870
BccI CCATC 5 cut(s) 260, 890, 989, 1013, 1736
BceAI ACGGC 3 cut(s) 171, 721, 1453
BciT130I CCWGG 1 cut(s) 1443
BciVI GTATCC 1 cut(s) 662
BclI TGATCA 2 cut(s) 456, 1365
BcnI CCSGG 2 cut(s) 1203, 1204
BfaI CTAG 2 cut(s) 396, 638
BfmI CTRYAG 1 cut(s) 912
BfuAI ACCTGC 1 cut(s) 632
BfuI GTATCC 1 cut(s) 662
BlpI GCTNAGC 1 cut(s) 1631
Bme1390I CCNGG 3 cut(s) 1203, 1204, 1443
BmeT110I CYCGRG 2 cut(s) 756, 1202
BmgT120I GGNCC 1 cut(s) 1200
BmiI GGNNCC 1 cut(s) 1263
BmrFI CCNGG 3 cut(s) 1203, 1204, 1443
BmsI GCATC 2 cut(s) 721, 1517
BmtI GCTAGC 1 cut(s) 641
BpiI GAAGAC 2 cut(s) 234, 870
BplI GAGNNNNNCTC 2 cut(s) 53, 85
BpmI CTGGAG 3 cut(s) 103, 234, 1757
Bpu1102I GCTNAGC 1 cut(s) 1631
BpuEI CTTGAG 2 cut(s) 1174, 1293
BpuMI CCSGG 2 cut(s) 1203, 1204
BsaJI CCNNGG 2 cut(s) 1202, 1203
BsaXI ACNNNNNCTCC 2 cut(s) 1020, 1050
Bse1I ACTGG 4 cut(s) 86, 251, 713, 1344
Bse3DI GCAATG 2 cut(s) 1731, 1737
BseBI CCWGG 1 cut(s) 1443
BseDI CCNNGG 2 cut(s) 1202, 1203
BseGI GGATG 1 cut(s) 736
BseMI GCAATG 2 cut(s) 1731, 1737
BseMII CTCAG 2 cut(s) 84, 1557
BseNI ACTGG 4 cut(s) 86, 251, 713, 1344
BseRI GAGGAG 1 cut(s) 1466
BsgI GTGCAG 1 cut(s) 1251
Bsh1236I CGCG 2 cut(s) 540, 1115
Bsh1285I CGRYCG 1 cut(s) 1113
BshFI GGCC 5 cut(s) 708, 980, 1004, 1201, 1353
BshNI GGYRCC 1 cut(s) 1261
BsiEI CGRYCG 1 cut(s) 1113
BsiHKCI CYCGRG 2 cut(s) 756, 1202
BsiSI CCGG 1 cut(s) 1203
BsmI GAATGC 1 cut(s) 1490
BsnI GGCC 5 cut(s) 708, 980, 1004, 1201, 1353
BsoBI CYCGRG 2 cut(s) 756, 1202
Bsp143I GATC 7 cut(s) 409, 456, 598, 960, 1110, 1365, 1497
Bsp1720I GCTNAGC 1 cut(s) 1631
Bsp68I TCGCGA 1 cut(s) 1115
BspACI CCGC 3 cut(s) 540, 1446, 1677
BspANI GGCC 5 cut(s) 708, 980, 1004, 1201, 1353
BspCNI CTCAG 2 cut(s) 83, 1556
BspFNI CGCG 2 cut(s) 540, 1115
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 1 cut(s) 1263
BspMAI CTGCAG 1 cut(s) 916
BspMI ACCTGC 1 cut(s) 632
BspOI GCTAGC 1 cut(s) 641
BspPI GGATC 2 cut(s) 606, 968
BspQI GCTCTTC 1 cut(s) 165
BspT107I GGYRCC 1 cut(s) 1261
BsrDI GCAATG 2 cut(s) 1731, 1737
BsrI ACTGG 4 cut(s) 86, 251, 713, 1344
BssECI CCNNGG 2 cut(s) 1202, 1203
BssMI GATC 7 cut(s) 409, 456, 598, 960, 1110, 1365, 1497
Bst2UI CCWGG 1 cut(s) 1443
Bst4CI ACNGT 3 cut(s) 179, 1414, 1438
Bst6I CTCTTC 3 cut(s) 165, 363, 368
BstAPI GCANNNNNTGC 2 cut(s) 624, 1415
BstC8I GCNNGC 5 cut(s) 639, 1220, 1266, 1466, 1637
BstDEI CTNAG 6 cut(s) 70, 788, 803, 985, 1543, 1631
BstF5I GGATG 1 cut(s) 736
BstFNI CGCG 2 cut(s) 540, 1115
BstHHI GCGC 1 cut(s) 1827
BstKTI GATC 7 cut(s) 412, 459, 601, 963, 1113, 1368, 1500
BstMBI GATC 7 cut(s) 409, 456, 598, 960, 1110, 1365, 1497
BstMCI CGRYCG 1 cut(s) 1113
BstMWI GCNNNNNNNGC 4 cut(s) 624, 1415, 1615, 1636
BstNI CCWGG 1 cut(s) 1443
BstNSI RCATGY 2 cut(s) 500, 1639
BstSCI CCNGG 3 cut(s) 1201, 1202, 1441
BstSFI CTRYAG 1 cut(s) 912
BstUI CGCG 2 cut(s) 540, 1115
BstV2I GAAGAC 2 cut(s) 234, 870
BstXI CCANNNNNNTGG 1 cut(s) 853
BsuI GTATCC 1 cut(s) 662
BsuRI GGCC 5 cut(s) 708, 980, 1004, 1201, 1353
BtsCI GGATG 1 cut(s) 736
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 2 cut(s) 40, 1241
BtuMI TCGCGA 1 cut(s) 1115
BveI ACCTGC 1 cut(s) 632
Cac8I GCNNGC 5 cut(s) 639, 1220, 1266, 1466, 1637
CciI TCATGA 1 cut(s) 526
CfoI GCGC 1 cut(s) 1827
Cfr13I GGNCC 1 cut(s) 1200
Cfr9I CCCGGG 1 cut(s) 1202
Csp6I GTAC 1 cut(s) 1709
CviQI GTAC 1 cut(s) 1709
DdeI CTNAG 6 cut(s) 70, 788, 803, 985, 1543, 1631
DpnI GATC 7 cut(s) 411, 458, 600, 962, 1112, 1367, 1499
DpnII GATC 7 cut(s) 409, 456, 598, 960, 1110, 1365, 1497
Eam1104I CTCTTC 3 cut(s) 165, 363, 368
EarI CTCTTC 3 cut(s) 165, 363, 368
Eco32I GATATC 1 cut(s) 1602
Eco57I CTGAAG 2 cut(s) 879, 1634
Eco88I CYCGRG 2 cut(s) 756, 1202
EcoRII CCWGG 1 cut(s) 1441
EcoRV GATATC 1 cut(s) 1602
EcoT22I ATGCAT 1 cut(s) 1388
FauI CCCGC 1 cut(s) 533
FbaI TGATCA 2 cut(s) 456, 1365
FokI GGATG 1 cut(s) 743
FspBI CTAG 2 cut(s) 396, 638
FspI TGCGCA 1 cut(s) 1826
GlaI GCGC 1 cut(s) 1826
GsuI CTGGAG 3 cut(s) 103, 234, 1757
HaeIII GGCC 5 cut(s) 708, 980, 1004, 1201, 1353
HapII CCGG 1 cut(s) 1203
HhaI GCGC 1 cut(s) 1827
Hin6I GCGC 1 cut(s) 1825
HinP1I GCGC 1 cut(s) 1825
HindIII AAGCTT 4 cut(s) 476, 839, 1311, 1616
HinfI GANTC 6 cut(s) 200, 247, 783, 792, 820, 1057
HpaII CCGG 1 cut(s) 1203
HphI GGTGA 3 cut(s) 59, 1354, 1531
Hpy166II GTNNAC 3 cut(s) 742, 1320, 1806
Hpy8I GTNNAC 3 cut(s) 742, 1320, 1806
HpyAV CCTTC 6 cut(s) 68, 589, 719, 1339, 1343, 1778
HpyCH4III ACNGT 3 cut(s) 179, 1414, 1438
HpyCH4IV ACGT 2 cut(s) 1122, 1571
HpyF10VI GCNNNNNNNGC 4 cut(s) 624, 1415, 1615, 1636
HpyF3I CTNAG 6 cut(s) 70, 788, 803, 985, 1543, 1631
HpySE526I ACGT 2 cut(s) 1122, 1571
HspAI GCGC 1 cut(s) 1825
Ksp22I TGATCA 2 cut(s) 456, 1365
Kzo9I GATC 7 cut(s) 409, 456, 598, 960, 1110, 1365, 1497
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 2 cut(s) 1479, 1729
LweI GCATC 2 cut(s) 721, 1517
MaeI CTAG 2 cut(s) 396, 638
MaeII ACGT 2 cut(s) 1122, 1571
MaeIII GTNAC 1 cut(s) 1651
MalI GATC 7 cut(s) 411, 458, 600, 962, 1112, 1367, 1499
MboI GATC 7 cut(s) 409, 456, 598, 960, 1110, 1365, 1497
MluCI AATT 8 cut(s) 191, 236, 293, 341, 435, 566, 1097, 1102
MlyI GAGTC 2 cut(s) 241, 801
Mph1103I ATGCAT 1 cut(s) 1388
MseI TTAA 5 cut(s) 138, 284, 480, 1101, 1811
MspA1I CMGCKG 3 cut(s) 634, 917, 947
MspI CCGG 1 cut(s) 1203
MspR9I CCNGG 3 cut(s) 1203, 1204, 1443
Mva1269I GAATGC 1 cut(s) 1490
MvaI CCWGG 1 cut(s) 1443
MvnI CGCG 2 cut(s) 540, 1115
MwoI GCNNNNNNNGC 4 cut(s) 624, 1415, 1615, 1636
NciI CCSGG 2 cut(s) 1203, 1204
NdeII GATC 7 cut(s) 409, 456, 598, 960, 1110, 1365, 1497
NheI GCTAGC 1 cut(s) 637
NlaIV GGNNCC 1 cut(s) 1263
NmeAIII GCCGAG 1 cut(s) 1379
NmuCI GTSAC 1 cut(s) 1651
NruI TCGCGA 1 cut(s) 1115
NsbI TGCGCA 1 cut(s) 1826
NsiI ATGCAT 1 cut(s) 1388
NspI RCATGY 2 cut(s) 500, 1639
PaeI GCATGC 1 cut(s) 1639
PagI TCATGA 1 cut(s) 526
PciI ACATGT 1 cut(s) 496
PciSI GCTCTTC 1 cut(s) 165
PcsI WCGNNNNNNNCGW 1 cut(s) 1119
PctI GAATGC 1 cut(s) 1490
PfeI GAWTC 4 cut(s) 200, 783, 820, 1057
Ple19I CGATCG 1 cut(s) 1113
PleI GAGTC 2 cut(s) 241, 800
PpsI GAGTC 2 cut(s) 241, 800
PscI ACATGT 1 cut(s) 496
Psp6I CCWGG 1 cut(s) 1441
PspGI CCWGG 1 cut(s) 1441
PspN4I GGNNCC 1 cut(s) 1263
PspPI GGNCC 1 cut(s) 1200
PsrI GAACNNNNNNTAC 2 cut(s) 1383, 1415
PstI CTGCAG 1 cut(s) 916
PvuI CGATCG 1 cut(s) 1113
PvuII CAGCTG 3 cut(s) 634, 917, 947
RruI TCGCGA 1 cut(s) 1115
RsaI GTAC 1 cut(s) 1710
RsaNI GTAC 1 cut(s) 1709
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 5 cut(s) 138, 284, 480, 1101, 1811
Sau3AI GATC 7 cut(s) 409, 456, 598, 960, 1110, 1365, 1497
Sau96I GGNCC 1 cut(s) 1200
SchI GAGTC 2 cut(s) 241, 801
ScrFI CCNGG 3 cut(s) 1203, 1204, 1443
SfaNI GCATC 2 cut(s) 721, 1517
SfcI CTRYAG 1 cut(s) 912
SmaI CCCGGG 1 cut(s) 1204
SmlI CTYRAG 2 cut(s) 1189, 1308
SmoI CTYRAG 2 cut(s) 1189, 1308
SphI GCATGC 1 cut(s) 1639
Sse9I AATT 8 cut(s) 191, 236, 293, 341, 435, 566, 1097, 1102
SsiI CCGC 3 cut(s) 540, 1446, 1677
SspI AATATT 1 cut(s) 1795
SspMI CTAG 2 cut(s) 396, 638
StyD4I CCNGG 3 cut(s) 1201, 1202, 1441
TaaI ACNGT 3 cut(s) 179, 1414, 1438
TaiI ACGT 2 cut(s) 1125, 1574
TaqI TCGA 6 cut(s) 245, 326, 1008, 1086, 1134, 1867
TaqII GACCGA 1 cut(s) 464
TasI AATT 8 cut(s) 191, 236, 293, 341, 435, 566, 1097, 1102
TauI GCSGC 1 cut(s) 1679
TfiI GAWTC 4 cut(s) 200, 783, 820, 1057
Tru1I TTAA 5 cut(s) 138, 284, 480, 1101, 1811
Tru9I TTAA 5 cut(s) 138, 284, 480, 1101, 1811
TscAI CASTG 2 cut(s) 40, 1241
TseFI GTSAC 1 cut(s) 1651
Tsp45I GTSAC 1 cut(s) 1651
TspMI CCCGGG 1 cut(s) 1202
TspRI CASTG 2 cut(s) 40, 1241
XapI RAATTY 2 cut(s) 293, 1097
XceI RCATGY 2 cut(s) 500, 1639
XmaI CCCGGG 1 cut(s) 1202
XspI CTAG 2 cut(s) 396, 638
Zsp2I ATGCAT 1 cut(s) 1388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.