Rh5AG014900

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0008844
Physical Location & Seq
Forward (+)
1029832 .. 1033631
3800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG014900.1

Sequence Viewer

Length: 1434 bp
ATGTCGACTTATAAGACGAGAGCCATTTGTTATAATCTCTTCTTGAAAGGAAAGTGGGATAGCTTGAAAGAATATTACTTGAGTAACGACTTCAATATATGTTTGCCTCATACAGTGTTCCAGGATACCGCAGTTCATGTTGCAGTGTACAGTAACAACAAAGAACTGGTGCGGTCTCTGCTTGATAGAGTGTATGGAGATGATCTCAGACAATACTTTTTGGAAGGACGACCACCAATATTTCAGAAACAGAATGAATTTGGAAATACACCTCTTCACGAGGCAGCTTCGATAGGAAATGTCGACATGGTGGAGCTCATAATGAAATGTGATCGGGACCAACTGAAAACTGTAAACAAGAGAGGTGAGACACCTTTTTTTATGGCCGCAGTATATGGCAACACCAATGTGATCAAGCATTTGGTGAAGGAAGTCGAGAAAGATATCGATGCTATGGAGCATGCTGATGATCATGAGAGGCTATATTCCAATTATACCTCATCTCCCGACTCCACCATGCTTCATGAAGCTATTCGCGGCTATTACTTTGAAACAGCTCTGGAGTTAGTAAAACCAGAATATCACATAAGGACAGATCGGAAGGATCAAGATGGCATGACCTGTTTTCAGCTGCTAGCTAACATACCATCTGCTTTTAAGAGTGGATATTCCTTTGGCATATCGGAGAAACTGATCTATCTTTGCCTTCCAGTAGATGATTACAGCAAAAACAAGGACGAAACCAACAGTGTATCTCAGGTCCCTCCAAATACAGATTTGGAGCGTGGCGATCACAGCAAAGACAAGGACAGAAACAAAAATGTATCTCAATTCCCTCCAAGTCTAAACACAGCCCTCCAGAAGATCACAACAAAAATATACGTCAACCTTTGGAGCAACATTGCTGAAGGGATCCCATTTATTAAACATCTATGGCACGAAAAGAAGAAGCACGGCGTAGTGGTGGAGCTTTCAAAAATTCTGGCTGGAGGAGACAAGTCATGGCAGGATAACAACATTGATAGTCAAAATGACACTCCACTTCTGGCTGCTGCTAGGAATGGAATCATAGAAATCATCGAGGCCATTTTTAATCTGTTCCCACAGGCCATCGAGCATGAAAACCACAAAGGAGAGAACATATTTCATGTTGTTGTGAAGCATCGGAGGAGAAAGCTTTTGGAATATCTCCAAACTTGTCATGTTTCAGATTCGAGGCTCCTGTGGAAAATCAATCAAGATGGTGATGACATTTTGCATAAAGCAGCATATAAGAGTGAGTATACCTCAAGGGAGAGGCCAGGGGAAGCTCTTCGCTTGCAGTCAGATATTAACTGGTTTGAGGTGTTTTTTCTTATAGATGAGTTTCTTCATTATTGCTCTTTCTTTATTTTACAGATAGTTGTAAATTGTAATGACCAATTATGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

477

Amino Acids

55.17

Weight (kDa)

5.96

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 82 - 150 1.2e-10 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 12, 33
AccI GTMKAC 3 cut(s) 5, 303, 1283
AccII CGCG 1 cut(s) 537
AciI CCGC 4 cut(s) 129, 172, 387, 537
AclWI GGATC 3 cut(s) 612, 907, 920
AcoI YGGCCR 1 cut(s) 384
AcsI RAATTY 2 cut(s) 257, 978
AcuI CTGAAG 1 cut(s) 927
AfaI GTAC 1 cut(s) 149
AgsI TTSAA 5 cut(s) 46, 67, 94, 551, 975
AjnI CCWGG 2 cut(s) 120, 1300
AleI CACNNNNGTG 1 cut(s) 407
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 3 cut(s) 180, 362, 987
AlwI GGATC 3 cut(s) 612, 907, 920
AoxI GGCC 4 cut(s) 384, 1083, 1107, 1298
ApeKI GCWGC 5 cut(s) 284, 631, 1049, 1052, 1265
ApoI RAATTY 2 cut(s) 257, 978
Asp700I GAANNNNTTC 2 cut(s) 531, 1311
AspS9I GGNCC 2 cut(s) 337, 760
AsuHPI GGTGA 3 cut(s) 377, 436, 1256
AsuNHI GCTAGC 1 cut(s) 634
AvaII GGWCC 2 cut(s) 337, 760
BaeI ACNNNNGTAYC 2 cut(s) 117, 150
BamHI GGATCC 1 cut(s) 912
BanII GRGCYC 1 cut(s) 318
BauI CACGAG 1 cut(s) 278
Bbv12I GWGCWC 1 cut(s) 318
BbvI GCAGC 5 cut(s) 296, 618, 1036, 1039, 1277
BccI CCATC 4 cut(s) 605, 655, 1118, 1235
BceAI ACGGC 1 cut(s) 970
BciT130I CCWGG 2 cut(s) 122, 1302
BciVI GTATCC 1 cut(s) 118
BclI TGATCA 2 cut(s) 411, 469
BcoDI GTCTC 3 cut(s) 180, 362, 987
BfaI CTAG 2 cut(s) 635, 1056
BfuI GTATCC 1 cut(s) 118
BisI GCNGC 7 cut(s) 285, 387, 538, 632, 1050, 1053, 1266
BlsI GCNGC 7 cut(s) 286, 388, 539, 633, 1051, 1054, 1267
Bme1390I CCNGG 2 cut(s) 122, 1302
Bme18I GGWCC 2 cut(s) 337, 760
BmgT120I GGNCC 2 cut(s) 337, 760
BmiI GGNNCC 4 cut(s) 338, 762, 914, 1220
BmrFI CCNGG 2 cut(s) 122, 1302
BmsI GCATC 2 cut(s) 439, 1171
BmtI GCTAGC 1 cut(s) 638
BplI GAGNNNNNCTC 4 cut(s) 189, 221, 1271, 1303
BpmI CTGGAG 3 cut(s) 581, 842, 1008
BpuEI CTTGAG 2 cut(s) 100, 1273
Bsa29I ATCGAT 1 cut(s) 447
BsaI GGTCTC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 1301
BsaXI ACNNNNNCTCC 2 cut(s) 487, 517
Bse1I ACTGG 3 cut(s) 171, 710, 1340
Bse3DI GCAATG 1 cut(s) 900
BseBI CCWGG 2 cut(s) 122, 1302
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 1 cut(s) 1301
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 2 cut(s) 220, 770
BseNI ACTGG 3 cut(s) 171, 710, 1340
BseRI GAGGAG 2 cut(s) 1005, 1183
BseXI GCAGC 5 cut(s) 296, 618, 1036, 1039, 1277
Bsh1236I CGCG 1 cut(s) 537
BshFI GGCC 4 cut(s) 386, 1085, 1109, 1300
BshVI ATCGAT 1 cut(s) 447
BsiHKAI GWGCWC 1 cut(s) 318
BslFI GGGAC 2 cut(s) 350, 746
BsmAI GTCTC 3 cut(s) 180, 362, 987
BsmFI GGGAC 2 cut(s) 350, 746
BsnI GGCC 4 cut(s) 386, 1085, 1109, 1300
Bso31I GGTCTC 1 cut(s) 180
Bsp1286I GDGCHC 1 cut(s) 318
Bsp1407I TGTACA 1 cut(s) 147
BspACI CCGC 4 cut(s) 129, 172, 387, 537
BspANI GGCC 4 cut(s) 386, 1085, 1109, 1300
BspCNI CTCAG 2 cut(s) 219, 769
BspDI ATCGAT 1 cut(s) 447
BspFNI CGCG 1 cut(s) 537
BspHI TCATGA 2 cut(s) 472, 523
BspLI GGNNCC 4 cut(s) 338, 762, 914, 1220
BspOI GCTAGC 1 cut(s) 638
BspPI GGATC 3 cut(s) 612, 907, 920
BspQI GCTCTTC 1 cut(s) 1317
BspTNI GGTCTC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 900
BsrGI TGTACA 1 cut(s) 147
BsrI ACTGG 3 cut(s) 171, 710, 1340
BssECI CCNNGG 1 cut(s) 1301
BssNAI GTATAC 1 cut(s) 1284
BssSI CACGAG 1 cut(s) 278
Bst1107I GTATAC 1 cut(s) 1284
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 2 cut(s) 122, 1302
Bst4CI ACNGT 4 cut(s) 115, 152, 352, 749
Bst6I CTCTTC 3 cut(s) 44, 279, 1317
BstAUI TGTACA 1 cut(s) 147
BstC8I GCNNGC 3 cut(s) 462, 636, 1319
BstDEI CTNAG 2 cut(s) 206, 756
BstFNI CGCG 1 cut(s) 537
BstMAI GTCTC 3 cut(s) 180, 362, 987
BstMWI GCNNNNNNNGC 2 cut(s) 178, 795
BstNI CCWGG 2 cut(s) 122, 1302
BstNSI RCATGY 1 cut(s) 464
BstSCI CCNGG 2 cut(s) 120, 1300
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 5 cut(s) 296, 618, 1036, 1039, 1277
BstX2I RGATCY 1 cut(s) 912
BstYI RGATCY 1 cut(s) 912
BstZ17I GTATAC 1 cut(s) 1284
Bsu15I ATCGAT 1 cut(s) 447
BsuI GTATCC 1 cut(s) 118
BsuRI GGCC 4 cut(s) 386, 1085, 1109, 1300
BsuTUI ATCGAT 1 cut(s) 447
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 3 cut(s) 120, 150, 754
Cac8I GCNNGC 3 cut(s) 462, 636, 1319
CciI TCATGA 2 cut(s) 472, 523
Cfr13I GGNCC 2 cut(s) 337, 760
ClaI ATCGAT 1 cut(s) 447
Csp6I GTAC 1 cut(s) 148
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 2 cut(s) 206, 756
EaeI YGGCCR 1 cut(s) 384
Eam1104I CTCTTC 3 cut(s) 44, 279, 1317
EarI CTCTTC 3 cut(s) 44, 279, 1317
Ecl136II GAGCTC 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 318
Eco31I GGTCTC 1 cut(s) 180
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 2 cut(s) 337, 760
Eco53kI GAGCTC 1 cut(s) 316
Eco57I CTGAAG 1 cut(s) 927
EcoICRI GAGCTC 1 cut(s) 316
EcoO109I RGGNCCY 1 cut(s) 760
EcoRII CCWGG 2 cut(s) 120, 1300
EcoRV GATATC 1 cut(s) 445
EcoT38I GRGCYC 1 cut(s) 318
FaqI GGGAC 2 cut(s) 350, 746
FbaI TGATCA 2 cut(s) 411, 469
FblI GTMKAC 3 cut(s) 5, 303, 1283
Fnu4HI GCNGC 7 cut(s) 285, 387, 538, 632, 1050, 1053, 1266
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 7 cut(s) 285, 387, 538, 632, 1050, 1053, 1266
FspBI CTAG 2 cut(s) 635, 1056
GluI GCNGC 7 cut(s) 285, 387, 538, 632, 1050, 1053, 1266
GsuI CTGGAG 3 cut(s) 581, 842, 1008
HaeIII GGCC 4 cut(s) 386, 1085, 1109, 1300
HincII GTYRAC 3 cut(s) 6, 304, 886
HindII GTYRAC 3 cut(s) 6, 304, 886
HindIII AAGCTT 1 cut(s) 1175
HinfI GANTC 3 cut(s) 509, 1065, 1211
HphI GGTGA 3 cut(s) 377, 436, 1256
Hpy166II GTNNAC 6 cut(s) 6, 148, 304, 355, 886, 1284
Hpy188I TCNGA 7 cut(s) 209, 246, 600, 685, 1167, 1210, 1327
Hpy8I GTNNAC 6 cut(s) 6, 148, 304, 355, 886, 1284
HpyAV CCTTC 5 cut(s) 218, 421, 595, 716, 902
HpyCH4III ACNGT 4 cut(s) 115, 152, 352, 749
HpyCH4IV ACGT 1 cut(s) 882
HpyCH4V TGCA 4 cut(s) 143, 1258, 1321, 1430
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 795
HpyF3I CTNAG 2 cut(s) 206, 756
HpySE526I ACGT 1 cut(s) 882
Ksp22I TGATCA 2 cut(s) 411, 469
LguI GCTCTTC 1 cut(s) 1317
LmnI GCTCC 6 cut(s) 313, 457, 781, 894, 967, 1224
Lsp1109I GCAGC 5 cut(s) 296, 618, 1036, 1039, 1277
LweI GCATC 2 cut(s) 439, 1171
MaeI CTAG 2 cut(s) 635, 1056
MaeII ACGT 1 cut(s) 882
MaeIII GTNAC 2 cut(s) 83, 152
MboII GAAGA 6 cut(s) 31, 266, 874, 958, 1304, 1361
MflI RGATCY 1 cut(s) 912
MhlI GDGCHC 1 cut(s) 318
MluCI AATT 6 cut(s) 257, 490, 830, 978, 1408, 1421
MlyI GAGTC 1 cut(s) 503
MroXI GAANNNNTTC 2 cut(s) 531, 1311
MseI TTAA 4 cut(s) 657, 924, 1092, 1332
MslI CAYNNNNRTG 2 cut(s) 407, 465
MspA1I CMGCKG 1 cut(s) 631
MspR9I CCNGG 2 cut(s) 122, 1302
MvaI CCWGG 2 cut(s) 122, 1302
MvnI CGCG 1 cut(s) 537
MwoI GCNNNNNNNGC 2 cut(s) 178, 795
NheI GCTAGC 1 cut(s) 634
NlaIV GGNNCC 4 cut(s) 338, 762, 914, 1220
NspI RCATGY 1 cut(s) 464
OliI CACNNNNGTG 1 cut(s) 407
PaeI GCATGC 1 cut(s) 464
PagI TCATGA 2 cut(s) 472, 523
PciSI GCTCTTC 1 cut(s) 1317
PdmI GAANNNNTTC 2 cut(s) 531, 1311
PfeI GAWTC 2 cut(s) 1065, 1211
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 7 cut(s) 286, 388, 539, 633, 1051, 1054, 1267
PleI GAGTC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 503
PpuMI RGGWCCY 1 cut(s) 760
PsiI TTATAA 2 cut(s) 12, 33
Psp124BI GAGCTC 1 cut(s) 318
Psp5II RGGWCCY 1 cut(s) 760
Psp6I CCWGG 2 cut(s) 120, 1300
PspGI CCWGG 2 cut(s) 120, 1300
PspN4I GGNNCC 4 cut(s) 338, 762, 914, 1220
PspPI GGNCC 2 cut(s) 337, 760
PspPPI RGGWCCY 1 cut(s) 760
PsuI RGATCY 1 cut(s) 912
PvuII CAGCTG 1 cut(s) 631
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
RseI CAYNNNNRTG 2 cut(s) 407, 465
SacI GAGCTC 1 cut(s) 318
SalI GTCGAC 2 cut(s) 4, 302
SapI GCTCTTC 1 cut(s) 1317
SaqAI TTAA 4 cut(s) 657, 924, 1092, 1332
SatI GCNGC 7 cut(s) 285, 387, 538, 632, 1050, 1053, 1266
Sau96I GGNCC 2 cut(s) 337, 760
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 2 cut(s) 122, 1302
SduI GDGCHC 1 cut(s) 318
SfaNI GCATC 2 cut(s) 439, 1171
SinI GGWCC 2 cut(s) 337, 760
SmiMI CAYNNNNRTG 2 cut(s) 407, 465
SmlI CTYRAG 2 cut(s) 79, 1288
SmoI CTYRAG 2 cut(s) 79, 1288
SphI GCATGC 1 cut(s) 464
Sse9I AATT 6 cut(s) 257, 490, 830, 978, 1408, 1421
SsiI CCGC 4 cut(s) 129, 172, 387, 537
SspI AATATT 2 cut(s) 74, 240
SspMI CTAG 2 cut(s) 635, 1056
SstI GAGCTC 1 cut(s) 318
StyD4I CCNGG 2 cut(s) 120, 1300
TaaI ACNGT 4 cut(s) 115, 152, 352, 749
TaiI ACGT 1 cut(s) 885
TaqI TCGA 8 cut(s) 5, 290, 303, 435, 447, 1080, 1113, 1214
TasI AATT 6 cut(s) 257, 490, 830, 978, 1408, 1421
TatI WGTACW 1 cut(s) 147
TauI GCSGC 2 cut(s) 389, 540
TfiI GAWTC 2 cut(s) 1065, 1211
Tru1I TTAA 4 cut(s) 657, 924, 1092, 1332
Tru9I TTAA 4 cut(s) 657, 924, 1092, 1332
TscAI CASTG 3 cut(s) 120, 150, 754
TseI GCWGC 5 cut(s) 284, 631, 1049, 1052, 1265
TspDTI ATGAA 8 cut(s) 125, 270, 338, 512, 540, 1134, 1136, 1361
TspRI CASTG 3 cut(s) 120, 150, 754
VpaK11BI GGWCC 2 cut(s) 337, 760
XapI RAATTY 2 cut(s) 257, 978
XceI RCATGY 1 cut(s) 464
XmiI GTMKAC 3 cut(s) 5, 303, 1283
XmnI GAANNNNTTC 2 cut(s) 531, 1311
XspI CTAG 2 cut(s) 635, 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.