Rorug04G0393600

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007033
Physical Location & Seq
Forward (+)
56001467 .. 56003533
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0393600.1

Sequence Viewer

Length: 540 bp
ATGGAGAGCTACCGACAACAAAAGATACAGAAATTGGAGGAGTTCGTCGATGGCCGCCTCAAACCTGACCTTGTTCAAACCATCGCTCAACGGGACAAGGTGTTTGAAAAGCAAAAAGTTTTCTCGGATTTGCGAAAGAACATTGAGAACTTGGAGAAAAATAGTGTAACCAGTCTTAGGACGATGGTAAATCTTGGTTCTGAAGTTTATATGCAAGCTGAAGTGCCAGATGCGCGACACATATTTGTAGATGTTGGACTTGGATTCCATGTGGAATTTACCTGGTCTGAAGCTTTGAATTACATATCTCAAAGAGAAGAAAAGTTAGAGAGGTACAAGCTTTTGTTTTCAAGTGTTCGGAGTTGTTTCTTTTTCTTTCGAAGAAAACTAACCATTCTTCAGAGGGTAGATAATTGGGAAATTGAAGATTATACCAACCGCATTGCCTCGATTAAATCCCACATAAAGATGGTATGTGAAGGGATTCGGGAATTACTCCAGCTTCCAGCAGAGAAACCTGTGCCAGAGCTCAACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

179

Amino Acids

21.28

Weight (kDa)

7.75

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin PF02996 29 - 111 3.7e-16 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 235
AciI CCGC 2 cut(s) 55, 439
AcoI YGGCCR 1 cut(s) 52
AcsI RAATTY 1 cut(s) 275
AcuI CTGAAG 4 cut(s) 222, 240, 309, 383
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 1 cut(s) 177
AgsI TTSAA 5 cut(s) 77, 107, 298, 351, 425
AjnI CCWGG 1 cut(s) 281
AluBI AGCT 6 cut(s) 9, 218, 293, 340, 502, 529
AluI AGCT 6 cut(s) 9, 218, 293, 340, 502, 529
Alw21I GWGCWC 1 cut(s) 531
AoxI GGCC 1 cut(s) 52
ApoI RAATTY 1 cut(s) 275
ArsI GACNNNNNNTTYG 2 cut(s) 86, 118
Asp700I GAANNNNTTC 1 cut(s) 483
AspLEI GCGC 1 cut(s) 235
AsuII TTCGAA 1 cut(s) 379
BanII GRGCYC 1 cut(s) 531
Bbv12I GWGCWC 1 cut(s) 531
BccI CCATC 4 cut(s) 44, 89, 178, 463
BciT130I CCWGG 1 cut(s) 283
BisI GCNGC 1 cut(s) 55
BlsI GCNGC 1 cut(s) 56
Bme1390I CCNGG 1 cut(s) 283
BmrFI CCNGG 1 cut(s) 283
BmsI GCATC 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 482
Bpu14I TTCGAA 1 cut(s) 379
Bsc4I CCNNNNNNNGG 1 cut(s) 177
Bse1I ACTGG 1 cut(s) 171
Bse3DI GCAATG 1 cut(s) 441
BseBI CCWGG 1 cut(s) 283
BseLI CCNNNNNNNGG 1 cut(s) 177
BseMI GCAATG 1 cut(s) 441
BseNI ACTGG 1 cut(s) 171
BseRI GAGGAG 1 cut(s) 53
Bsh1236I CGCG 1 cut(s) 235
BshFI GGCC 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 531
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 107
BsnI GGCC 1 cut(s) 54
Bsp119I TTCGAA 1 cut(s) 379
Bsp1286I GDGCHC 1 cut(s) 531
BspACI CCGC 2 cut(s) 55, 439
BspANI GGCC 1 cut(s) 54
BspFNI CGCG 1 cut(s) 235
BspT104I TTCGAA 1 cut(s) 379
BsrDI GCAATG 1 cut(s) 441
BsrI ACTGG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 283
BstBI TTCGAA 1 cut(s) 379
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 1 cut(s) 176
BstFNI CGCG 1 cut(s) 235
BstHHI GCGC 1 cut(s) 235
BstMWI GCNNNNNNNGC 1 cut(s) 232
BstNI CCWGG 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 281
BstUI CGCG 1 cut(s) 235
BsuRI GGCC 1 cut(s) 54
BtgZI GCGATG 1 cut(s) 67
Cac8I GCNNGC 1 cut(s) 216
CfoI GCGC 1 cut(s) 235
CsiI ACCWGGT 1 cut(s) 281
Csp6I GTAC 1 cut(s) 334
CviAII CATG 1 cut(s) 269
CviJI RGCY 7 cut(s) 9, 54, 218, 293, 340, 502, 529
CviKI_1 RGCY 7 cut(s) 9, 54, 218, 293, 340, 502, 529
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 1 cut(s) 176
EaeI YGGCCR 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 529
Eco24I GRGCYC 1 cut(s) 531
Eco53kI GAGCTC 1 cut(s) 529
Eco57I CTGAAG 4 cut(s) 222, 240, 309, 383
EcoICRI GAGCTC 1 cut(s) 529
EcoRII CCWGG 1 cut(s) 281
EcoT38I GRGCYC 1 cut(s) 531
FaeI CATG 1 cut(s) 272
FaiI YATR 8 cut(s) 210, 212, 242, 270, 305, 432, 464, 475
FaqI GGGAC 1 cut(s) 107
FatI CATG 1 cut(s) 268
Fnu4HI GCNGC 1 cut(s) 55
FriOI GRGCYC 1 cut(s) 531
Fsp4HI GCNGC 1 cut(s) 55
GlaI GCGC 1 cut(s) 234
GluI GCNGC 1 cut(s) 55
GsuI CTGGAG 1 cut(s) 482
HaeIII GGCC 1 cut(s) 54
HhaI GCGC 1 cut(s) 235
Hin1II CATG 1 cut(s) 272
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HindIII AAGCTT 2 cut(s) 291, 338
HinfI GANTC 2 cut(s) 264, 484
Hpy188I TCNGA 5 cut(s) 127, 202, 289, 360, 402
Hpy188III TCNNGA 1 cut(s) 488
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 1 cut(s) 473
HpyCH4V TGCA 1 cut(s) 214
HpyF10VI GCNNNNNNNGC 1 cut(s) 232
HpyF3I CTNAG 1 cut(s) 176
Hsp92II CATG 1 cut(s) 272
HspAI GCGC 1 cut(s) 233
LpnPI CCDG 8 cut(s) 78, 184, 240, 268, 295, 512, 519, 531
LweI GCATC 1 cut(s) 220
MabI ACCWGGT 1 cut(s) 281
MaeIII GTNAC 1 cut(s) 166
MboII GAAGA 4 cut(s) 329, 389, 393, 437
MhlI GDGCHC 1 cut(s) 531
MluCI AATT 6 cut(s) 32, 275, 298, 412, 420, 491
MmeI TCCRAC 1 cut(s) 235
MnlI CCTC 5 cut(s) 31, 68, 324, 396, 457
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 1 cut(s) 467
MspR9I CCNGG 1 cut(s) 283
MvaI CCWGG 1 cut(s) 283
MvnI CGCG 1 cut(s) 235
MwoI GCNNNNNNNGC 1 cut(s) 232
NlaIII CATG 1 cut(s) 272
NspV TTCGAA 1 cut(s) 379
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 2 cut(s) 264, 484
PkrI GCNGC 1 cut(s) 56
Psp124BI GAGCTC 1 cut(s) 531
Psp6I CCWGG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 281
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 1 cut(s) 531
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 1 cut(s) 55
ScrFI CCNGG 1 cut(s) 283
SduI GDGCHC 1 cut(s) 531
SexAI ACCWGGT 1 cut(s) 281
SfaNI GCATC 1 cut(s) 220
SfuI TTCGAA 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 467
Sse9I AATT 6 cut(s) 32, 275, 298, 412, 420, 491
SsiI CCGC 2 cut(s) 55, 439
SstI GAGCTC 1 cut(s) 531
StyD4I CCNGG 1 cut(s) 281
TaqI TCGA 3 cut(s) 48, 379, 449
TasI AATT 6 cut(s) 32, 275, 298, 412, 420, 491
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 2 cut(s) 264, 484
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
XapI RAATTY 1 cut(s) 275
XmnI GAANNNNTTC 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.