Rh5CG015800

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0007033
Physical Location & Seq
Reverse (-)
976669 .. 978753
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG015800.1

Sequence Viewer

Length: 633 bp
ATGGACGACGGCGATGCGGCGACGGCCGGGAGGAGGAGCTTCAAGCAGCGGCTGAGATTGACCGGAATCGGGTGCTGCGGCGCCGTCTGGGGATTTGGACGGACGGCCACGATTACCGTCGAGGACGATGAAGAACAACGCCTACATCCGCCGCAACAACAACCGCGGCAGCAGCTGCCGAACCTATCGCAGCTGCTGATTCTGGGGCAGGCGGCCGGGTCCGATTCGGATCCGGGTTGCCTGGGTCCGGTCAACCCCGGGTCGGGGATGAACCTCGCCGCGGCTCTGGCGGCGGAACGGCATTATCGGGAGGCGGACGAGGCCGCGTCGGGCGTTGGGGCGGCTCCGGGGACGCCGATGAGGGTGTCGCTGATGAGATTGCTGGAGGAGACGGAAATGGACGGGTGCGATGCGGCGGAGGAGAAAAATGAGAAAGCGGCGGCGGCGGCGGCGGCGACGGGGGTGGTGGGTTGTGATTCGGTGTGCTGCGTGTGCATGGGGAGGAAGAAAGGCGCGGCGTTCATACCATGTGGGCACACTTTTTGCAGGGTGTGTTCGAGGGAGGTGTGGCTGAATCGAGGCTCCTGTCCCCTCTGCAACCGTACGATCCTCGAGATTCTCGATATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

210

Amino Acids

22.17

Weight (kDa)

5.27

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 160 - 204 4.7e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 162 - 199 1.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 162 - 199 1.3e-06 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 162 - 203 5.9e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 80
AccII CGCG 4 cut(s) 166, 281, 326, 515
AclWI GGATC 3 cut(s) 224, 237, 601
AcoI YGGCCR 3 cut(s) 24, 105, 213
AcyI GRCGYC 2 cut(s) 81, 353
AfaI GTAC 1 cut(s) 604
AfiI CCNNNNNNNGG 7 cut(s) 33, 69, 247, 262, 263, 264, 280
AgsI TTSAA 1 cut(s) 43
AjnI CCWGG 1 cut(s) 240
AluBI AGCT 3 cut(s) 39, 175, 193
AluI AGCT 3 cut(s) 39, 175, 193
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 3 cut(s) 224, 237, 601
AlwNI CAGNNNCTG 3 cut(s) 52, 175, 196
Ama87I CYCGRG 2 cut(s) 257, 611
AoxI GGCC 4 cut(s) 24, 105, 213, 321
ApeKI GCWGC 8 cut(s) 46, 75, 169, 172, 175, 190, 193, 486
AspLEI GCGC 2 cut(s) 83, 515
AspS9I GGNCC 2 cut(s) 219, 245
AsuC2I CCSGG 6 cut(s) 28, 217, 234, 258, 259, 348
AvaI CYCGRG 2 cut(s) 257, 611
AvaII GGWCC 2 cut(s) 219, 245
BaeGI GKGCMC 1 cut(s) 537
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 1 cut(s) 80
BbvI GCAGC 8 cut(s) 58, 62, 162, 180, 181, 184, 202, 473
BceAI ACGGC 5 cut(s) 25, 39, 68, 120, 314
BcgI CGANNNNNNTGC 1 cut(s) 30
BciT130I CCWGG 1 cut(s) 242
BcnI CCSGG 6 cut(s) 28, 217, 234, 258, 259, 348
BcoDI GTCTC 1 cut(s) 383
BfoI RGCGCY 1 cut(s) 84
Bme1390I CCNGG 7 cut(s) 28, 217, 234, 242, 258, 259, 348
Bme18I GGWCC 2 cut(s) 219, 245
BmeT110I CYCGRG 2 cut(s) 257, 611
BmgT120I GGNCC 2 cut(s) 219, 245
BmiI GGNNCC 6 cut(s) 82, 220, 231, 246, 345, 583
BmrFI CCNGG 7 cut(s) 28, 217, 234, 242, 258, 259, 348
BmsI GCATC 2 cut(s) 4, 400
BpmI CTGGAG 1 cut(s) 404
BpuMI CCSGG 6 cut(s) 28, 217, 234, 258, 259, 348
BsaBI GATNNNNATC 1 cut(s) 228
BsaHI GRCGYC 2 cut(s) 81, 353
BsaJI CCNNGG 6 cut(s) 164, 241, 256, 257, 279, 347
BsaWI WCCGGW 2 cut(s) 62, 247
Bsc4I CCNNNNNNNGG 7 cut(s) 33, 69, 247, 262, 263, 264, 280
Bse8I GATNNNNATC 1 cut(s) 228
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 6 cut(s) 164, 241, 256, 257, 279, 347
BseGI GGATG 2 cut(s) 145, 273
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 7 cut(s) 33, 69, 247, 262, 263, 264, 280
BseMII CTCAG 1 cut(s) 44
BseRI GAGGAG 4 cut(s) 46, 49, 401, 434
BseSI GKGCMC 1 cut(s) 537
BseX3I CGGCCG 2 cut(s) 24, 213
BseXI GCAGC 8 cut(s) 58, 62, 162, 180, 181, 184, 202, 473
Bsh1236I CGCG 4 cut(s) 166, 281, 326, 515
Bsh1285I CGRYCG 2 cut(s) 27, 216
BshFI GGCC 4 cut(s) 26, 107, 215, 323
BshNI GGYRCC 1 cut(s) 80
BsiEI CGRYCG 2 cut(s) 27, 216
BsiHKCI CYCGRG 2 cut(s) 257, 611
BsiSI CCGG 7 cut(s) 27, 63, 216, 233, 248, 258, 347
BsiWI CGTACG 1 cut(s) 602
BslFI GGGAC 2 cut(s) 364, 573
BslI CCNNNNNNNGG 7 cut(s) 33, 69, 247, 262, 263, 264, 280
BsmAI GTCTC 1 cut(s) 383
BsmBI CGTCTC 1 cut(s) 383
BsmFI GGGAC 2 cut(s) 364, 573
BsnI GGCC 4 cut(s) 26, 107, 215, 323
BsoBI CYCGRG 2 cut(s) 257, 611
Bsp1286I GDGCHC 1 cut(s) 537
Bsp143I GATC 2 cut(s) 229, 606
BspANI GGCC 4 cut(s) 26, 107, 215, 323
BspCNI CTCAG 1 cut(s) 45
BspFNI CGCG 4 cut(s) 166, 281, 326, 515
BspLI GGNNCC 6 cut(s) 82, 220, 231, 246, 345, 583
BspPI GGATC 3 cut(s) 224, 237, 601
BspT107I GGYRCC 1 cut(s) 80
BssECI CCNNGG 6 cut(s) 164, 241, 256, 257, 279, 347
BssMI GATC 2 cut(s) 229, 606
BssNI GRCGYC 2 cut(s) 81, 353
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 2 cut(s) 118, 602
BstACI GRCGYC 2 cut(s) 81, 353
BstAPI GCANNNNNTGC 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 1 cut(s) 53
BstDSI CCRYGG 2 cut(s) 164, 279
BstF5I GGATG 2 cut(s) 145, 273
BstFNI CGCG 4 cut(s) 166, 281, 326, 515
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 2 cut(s) 83, 515
BstKTI GATC 2 cut(s) 232, 609
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 2 cut(s) 229, 606
BstMCI CGRYCG 2 cut(s) 27, 216
BstNI CCWGG 1 cut(s) 242
BstSCI CCNGG 7 cut(s) 26, 215, 232, 240, 256, 257, 346
BstSLI GKGCMC 1 cut(s) 537
BstUI CGCG 4 cut(s) 166, 281, 326, 515
BstV1I GCAGC 8 cut(s) 58, 62, 162, 180, 181, 184, 202, 473
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BstZI CGGCCG 2 cut(s) 24, 213
BsuRI GGCC 4 cut(s) 26, 107, 215, 323
BtgI CCRYGG 2 cut(s) 164, 279
BtgZI GCGATG 2 cut(s) 27, 423
BtsCI GGATG 2 cut(s) 145, 273
Cac8I GCNNGC 1 cut(s) 210
CaiI CAGNNNCTG 3 cut(s) 52, 175, 196
CfoI GCGC 2 cut(s) 83, 515
Cfr13I GGNCC 2 cut(s) 219, 245
Cfr42I CCGCGG 2 cut(s) 167, 282
Cfr9I CCCGGG 1 cut(s) 257
CseI GACGC 2 cut(s) 315, 361
Csp6I GTAC 1 cut(s) 603
CviAII CATG 2 cut(s) 496, 528
CviQI GTAC 1 cut(s) 603
DdeI CTNAG 1 cut(s) 53
DinI GGCGCC 1 cut(s) 82
DpnI GATC 2 cut(s) 231, 608
DpnII GATC 2 cut(s) 229, 606
EaeI YGGCCR 3 cut(s) 24, 105, 213
EagI CGGCCG 2 cut(s) 24, 213
EciI GGCGGA 4 cut(s) 138, 308, 329, 431
EclXI CGGCCG 2 cut(s) 24, 213
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 2 cut(s) 219, 245
Eco52I CGGCCG 2 cut(s) 24, 213
Eco88I CYCGRG 2 cut(s) 257, 611
EcoRII CCWGG 1 cut(s) 240
EcoRV GATATC 1 cut(s) 625
EgeI GGCGCC 1 cut(s) 82
EheI GGCGCC 1 cut(s) 82
Esp3I CGTCTC 1 cut(s) 383
FaeI CATG 2 cut(s) 499, 531
FaiI YATR 3 cut(s) 497, 524, 529
FaqI GGGAC 2 cut(s) 364, 573
FatI CATG 2 cut(s) 495, 527
FokI GGATG 2 cut(s) 132, 280
GlaI GCGC 2 cut(s) 82, 514
GsuI CTGGAG 1 cut(s) 404
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 4 cut(s) 26, 107, 215, 323
HapII CCGG 7 cut(s) 27, 63, 216, 233, 248, 258, 347
HgaI GACGC 2 cut(s) 315, 361
HhaI GCGC 2 cut(s) 83, 515
Hin1I GRCGYC 2 cut(s) 81, 353
Hin1II CATG 2 cut(s) 499, 531
Hin6I GCGC 2 cut(s) 81, 513
HinP1I GCGC 2 cut(s) 81, 513
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 6 cut(s) 66, 199, 224, 476, 574, 616
HpaII CCGG 7 cut(s) 27, 63, 216, 233, 248, 258, 347
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 3 cut(s) 223, 229, 632
Hpy188III TCNNGA 3 cut(s) 308, 613, 620
Hpy8I GTNNAC 1 cut(s) 253
Hpy99I CGWCG 5 cut(s) 11, 25, 122, 331, 460
HpyCH4III ACNGT 2 cut(s) 118, 602
HpyCH4V TGCA 3 cut(s) 495, 546, 597
HpyF3I CTNAG 1 cut(s) 53
Hsp92I GRCGYC 2 cut(s) 81, 353
Hsp92II CATG 2 cut(s) 499, 531
HspAI GCGC 2 cut(s) 81, 513
KasI GGCGCC 1 cut(s) 80
KspI CCGCGG 2 cut(s) 167, 282
Kzo9I GATC 2 cut(s) 229, 606
LmnI GCTCC 3 cut(s) 36, 349, 587
Lsp1109I GCAGC 8 cut(s) 58, 62, 162, 180, 181, 184, 202, 473
LweI GCATC 2 cut(s) 4, 400
MalI GATC 2 cut(s) 231, 608
MboI GATC 2 cut(s) 229, 606
MboII GAAGA 3 cut(s) 143, 517, 619
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 537
Mly113I GGCGCC 1 cut(s) 81
MspA1I CMGCKG 5 cut(s) 49, 166, 175, 193, 281
MspI CCGG 7 cut(s) 27, 63, 216, 233, 248, 258, 347
MspR9I CCNGG 7 cut(s) 28, 217, 234, 242, 258, 259, 348
MvaI CCWGG 1 cut(s) 242
MvnI CGCG 4 cut(s) 166, 281, 326, 515
NarI GGCGCC 1 cut(s) 81
NciI CCSGG 6 cut(s) 28, 217, 234, 258, 259, 348
NdeII GATC 2 cut(s) 229, 606
NlaIII CATG 2 cut(s) 499, 531
NlaIV GGNNCC 6 cut(s) 82, 220, 231, 246, 345, 583
PaeR7I CTCGAG 1 cut(s) 611
PcsI WCGNNNNNNNCGW 1 cut(s) 618
PfeI GAWTC 6 cut(s) 66, 199, 224, 476, 574, 616
Pfl23II CGTACG 1 cut(s) 602
PluTI GGCGCC 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PspLI CGTACG 1 cut(s) 602
PspN4I GGNNCC 6 cut(s) 82, 220, 231, 246, 345, 583
PspPI GGNCC 2 cut(s) 219, 245
PsrI GAACNNNNNNTAC 2 cut(s) 126, 158
PstNI CAGNNNCTG 3 cut(s) 52, 175, 196
PsuI RGATCY 1 cut(s) 229
PvuII CAGCTG 2 cut(s) 175, 193
RsaI GTAC 1 cut(s) 604
RsaNI GTAC 1 cut(s) 603
SacII CCGCGG 2 cut(s) 167, 282
Sau3AI GATC 2 cut(s) 229, 606
Sau96I GGNCC 2 cut(s) 219, 245
ScrFI CCNGG 7 cut(s) 28, 217, 234, 242, 258, 259, 348
SduI GDGCHC 1 cut(s) 537
SetI ASST 6 cut(s) 41, 177, 186, 195, 276, 567
SfaNI GCATC 2 cut(s) 4, 400
SfoI GGCGCC 1 cut(s) 82
Sfr274I CTCGAG 1 cut(s) 611
Sfr303I CCGCGG 2 cut(s) 167, 282
SgrBI CCGCGG 2 cut(s) 167, 282
SinI GGWCC 2 cut(s) 219, 245
SlaI CTCGAG 1 cut(s) 611
SmaI CCCGGG 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
SspDI GGCGCC 1 cut(s) 80
StyD4I CCNGG 7 cut(s) 26, 215, 232, 240, 256, 257, 346
TaaI ACNGT 2 cut(s) 118, 602
TaqI TCGA 5 cut(s) 120, 557, 577, 612, 621
TfiI GAWTC 6 cut(s) 66, 199, 224, 476, 574, 616
TseI GCWGC 8 cut(s) 46, 75, 169, 172, 175, 190, 193, 486
TspDTI ATGAA 3 cut(s) 144, 284, 511
TspGWI ACGGA 2 cut(s) 115, 407
TspMI CCCGGG 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 219, 245
XhoI CTCGAG 1 cut(s) 611
XmaI CCCGGG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.