Rh5BG018100

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007033
Physical Location & Seq
Reverse (-)
1312690 .. 1314672
1983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG018100.1

Sequence Viewer

Length: 627 bp
ATGGACGACGGCGATGTGGCCACGGCGGGGAGGAGGAGCTTCAAGCAGCGGCTGAGATTGACCGGAATCGGGTGCTGCGGCGCCGTCTGGGGATTTGGACGGACGGCCACGATCACCGTCGAGGACGATGAAGAACAACACGTACATCCGCCGCAACAACAACCGCGGCAGCAGCTGCCGAACCTATCGCAGCTGCTGATTCTGGGGCAGGCGGCCGGGTCCGATTCGGATCCGGGTTGCCTGGGTCCGGTCAACTCCGGGTCGGGGATGAACCTCGCCGCGGCTCTGGCGGCGGAACGGCATTATCGGGAAGCGGACGAGGCCGCGTCGGGTGGTGGGGCGGCGCCGGGGACGCCGATGAGGGTGTCGCTGATGAGATTGCTGGAGGAGACGGAAATGGACGGGTGCGATGCGGCGGAGGAGAAGAATGAGAAAGCGGCGGCGGCGGCGGCGGGGGTGGTGGGTTGTGATTCGGTGTGCTGCGTGTGCATGGGGAGGAAGAAAGGCGCGGCGTTCATACCATGTGGGCACACTTTTTGCAGGGTGTGTTCGAGGGAGGTGTGGCTGAATCGAGGCTCCTGTCCCCTCTGCAACCGTACGATCCTCGAGATTCTCGATATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

208

Amino Acids

21.94

Weight (kDa)

5.25

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 158 - 202 4.6e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 160 - 197 1.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 160 - 197 1.3e-06 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 160 - 201 5.9e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 80, 343
AccII CGCG 4 cut(s) 166, 281, 326, 509
AclWI GGATC 3 cut(s) 224, 237, 595
AcoI YGGCCR 3 cut(s) 18, 105, 213
AcyI GRCGYC 3 cut(s) 81, 344, 353
AfaI GTAC 2 cut(s) 144, 598
AfiI CCNNNNNNNGG 5 cut(s) 27, 69, 247, 264, 280
AflIII ACRYGT 1 cut(s) 139
AgsI TTSAA 1 cut(s) 43
AjnI CCWGG 1 cut(s) 240
AluBI AGCT 3 cut(s) 39, 175, 193
AluI AGCT 3 cut(s) 39, 175, 193
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 3 cut(s) 224, 237, 595
AlwNI CAGNNNCTG 3 cut(s) 52, 175, 196
Ama87I CYCGRG 1 cut(s) 605
AoxI GGCC 4 cut(s) 18, 105, 213, 321
ApeKI GCWGC 8 cut(s) 46, 75, 169, 172, 175, 190, 193, 480
AspLEI GCGC 3 cut(s) 83, 346, 509
AspS9I GGNCC 2 cut(s) 219, 245
AsuC2I CCSGG 4 cut(s) 217, 234, 259, 348
AsuHPI GGTGA 1 cut(s) 106
AvaI CYCGRG 1 cut(s) 605
AvaII GGWCC 2 cut(s) 219, 245
BaeGI GKGCMC 1 cut(s) 531
BalI TGGCCA 1 cut(s) 20
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 2 cut(s) 80, 343
BbvI GCAGC 8 cut(s) 58, 62, 162, 180, 181, 184, 202, 467
BceAI ACGGC 5 cut(s) 25, 39, 68, 120, 314
BciT130I CCWGG 1 cut(s) 242
BcnI CCSGG 4 cut(s) 217, 234, 259, 348
BcoDI GTCTC 1 cut(s) 383
BfoI RGCGCY 2 cut(s) 84, 347
Bme1390I CCNGG 5 cut(s) 217, 234, 242, 259, 348
Bme18I GGWCC 2 cut(s) 219, 245
BmeT110I CYCGRG 1 cut(s) 605
BmgT120I GGNCC 2 cut(s) 219, 245
BmiI GGNNCC 6 cut(s) 82, 220, 231, 246, 345, 577
BmrFI CCNGG 5 cut(s) 217, 234, 242, 259, 348
BmsI GCATC 1 cut(s) 400
BpmI CTGGAG 1 cut(s) 404
BpuMI CCSGG 4 cut(s) 217, 234, 259, 348
BsaAI YACGTR 1 cut(s) 142
BsaBI GATNNNNATC 1 cut(s) 228
BsaHI GRCGYC 3 cut(s) 81, 344, 353
BsaJI CCNNGG 5 cut(s) 21, 164, 241, 279, 347
BsaWI WCCGGW 2 cut(s) 62, 247
Bsc4I CCNNNNNNNGG 5 cut(s) 27, 69, 247, 264, 280
Bse8I GATNNNNATC 1 cut(s) 228
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 5 cut(s) 21, 164, 241, 279, 347
BseGI GGATG 2 cut(s) 145, 273
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 5 cut(s) 27, 69, 247, 264, 280
BseMII CTCAG 1 cut(s) 44
BseRI GAGGAG 4 cut(s) 46, 49, 401, 434
BseSI GKGCMC 1 cut(s) 531
BseX3I CGGCCG 1 cut(s) 213
BseXI GCAGC 8 cut(s) 58, 62, 162, 180, 181, 184, 202, 467
Bsh1236I CGCG 4 cut(s) 166, 281, 326, 509
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 4 cut(s) 20, 107, 215, 323
BshNI GGYRCC 2 cut(s) 80, 343
BsiEI CGRYCG 1 cut(s) 216
BsiHKCI CYCGRG 1 cut(s) 605
BsiSI CCGG 6 cut(s) 63, 216, 233, 248, 258, 347
BsiWI CGTACG 1 cut(s) 596
BslFI GGGAC 2 cut(s) 364, 567
BslI CCNNNNNNNGG 5 cut(s) 27, 69, 247, 264, 280
BsmAI GTCTC 1 cut(s) 383
BsmBI CGTCTC 1 cut(s) 383
BsmFI GGGAC 2 cut(s) 364, 567
BsnI GGCC 4 cut(s) 20, 107, 215, 323
BsoBI CYCGRG 1 cut(s) 605
Bsp1286I GDGCHC 1 cut(s) 531
Bsp143I GATC 3 cut(s) 111, 229, 600
BspANI GGCC 4 cut(s) 20, 107, 215, 323
BspCNI CTCAG 1 cut(s) 45
BspFNI CGCG 4 cut(s) 166, 281, 326, 509
BspLI GGNNCC 6 cut(s) 82, 220, 231, 246, 345, 577
BspPI GGATC 3 cut(s) 224, 237, 595
BspT107I GGYRCC 2 cut(s) 80, 343
BssECI CCNNGG 5 cut(s) 21, 164, 241, 279, 347
BssMI GATC 3 cut(s) 111, 229, 600
BssNI GRCGYC 3 cut(s) 81, 344, 353
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 2 cut(s) 118, 596
BstACI GRCGYC 3 cut(s) 81, 344, 353
BstAPI GCANNNNNTGC 1 cut(s) 175
BstBAI YACGTR 1 cut(s) 142
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 1 cut(s) 53
BstDSI CCRYGG 3 cut(s) 21, 164, 279
BstF5I GGATG 2 cut(s) 145, 273
BstFNI CGCG 4 cut(s) 166, 281, 326, 509
BstH2I RGCGCY 2 cut(s) 84, 347
BstHHI GCGC 3 cut(s) 83, 346, 509
BstKTI GATC 3 cut(s) 114, 232, 603
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 3 cut(s) 111, 229, 600
BstMCI CGRYCG 1 cut(s) 216
BstNI CCWGG 1 cut(s) 242
BstSCI CCNGG 5 cut(s) 215, 232, 240, 257, 346
BstSLI GKGCMC 1 cut(s) 531
BstUI CGCG 4 cut(s) 166, 281, 326, 509
BstV1I GCAGC 8 cut(s) 58, 62, 162, 180, 181, 184, 202, 467
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BstZI CGGCCG 1 cut(s) 213
BsuRI GGCC 4 cut(s) 20, 107, 215, 323
BtgI CCRYGG 3 cut(s) 21, 164, 279
BtgZI GCGATG 2 cut(s) 27, 423
BtsCI GGATG 2 cut(s) 145, 273
Cac8I GCNNGC 1 cut(s) 210
CaiI CAGNNNCTG 3 cut(s) 52, 175, 196
CfoI GCGC 3 cut(s) 83, 346, 509
Cfr13I GGNCC 2 cut(s) 219, 245
Cfr42I CCGCGG 2 cut(s) 167, 282
CseI GACGC 2 cut(s) 315, 361
Csp6I GTAC 2 cut(s) 143, 597
CviAII CATG 2 cut(s) 490, 522
CviQI GTAC 2 cut(s) 143, 597
DdeI CTNAG 1 cut(s) 53
DinI GGCGCC 2 cut(s) 82, 345
DpnI GATC 3 cut(s) 113, 231, 602
DpnII GATC 3 cut(s) 111, 229, 600
EaeI YGGCCR 3 cut(s) 18, 105, 213
EagI CGGCCG 1 cut(s) 213
EciI GGCGGA 3 cut(s) 138, 308, 431
EclXI CGGCCG 1 cut(s) 213
Eco32I GATATC 1 cut(s) 619
Eco47I GGWCC 2 cut(s) 219, 245
Eco52I CGGCCG 1 cut(s) 213
Eco88I CYCGRG 1 cut(s) 605
EcoRII CCWGG 1 cut(s) 240
EcoRV GATATC 1 cut(s) 619
EgeI GGCGCC 2 cut(s) 82, 345
EheI GGCGCC 2 cut(s) 82, 345
Esp3I CGTCTC 1 cut(s) 383
FaeI CATG 2 cut(s) 493, 525
FaiI YATR 3 cut(s) 491, 518, 523
FaqI GGGAC 2 cut(s) 364, 567
FatI CATG 2 cut(s) 489, 521
FauI CCCGC 2 cut(s) 19, 445
FokI GGATG 2 cut(s) 132, 280
GlaI GCGC 3 cut(s) 82, 345, 508
GsuI CTGGAG 1 cut(s) 404
HaeII RGCGCY 2 cut(s) 84, 347
HaeIII GGCC 4 cut(s) 20, 107, 215, 323
HapII CCGG 6 cut(s) 63, 216, 233, 248, 258, 347
HgaI GACGC 2 cut(s) 315, 361
HhaI GCGC 3 cut(s) 83, 346, 509
Hin1I GRCGYC 3 cut(s) 81, 344, 353
Hin1II CATG 2 cut(s) 493, 525
Hin6I GCGC 3 cut(s) 81, 344, 507
HinP1I GCGC 3 cut(s) 81, 344, 507
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 6 cut(s) 66, 199, 224, 470, 568, 610
HpaII CCGG 6 cut(s) 63, 216, 233, 248, 258, 347
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 3 cut(s) 223, 229, 626
Hpy188III TCNNGA 3 cut(s) 308, 607, 614
Hpy8I GTNNAC 1 cut(s) 253
Hpy99I CGWCG 3 cut(s) 11, 122, 331
HpyCH4III ACNGT 2 cut(s) 118, 596
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 3 cut(s) 489, 540, 591
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 1 cut(s) 141
Hsp92I GRCGYC 3 cut(s) 81, 344, 353
Hsp92II CATG 2 cut(s) 493, 525
HspAI GCGC 3 cut(s) 81, 344, 507
KasI GGCGCC 2 cut(s) 80, 343
KspI CCGCGG 2 cut(s) 167, 282
Kzo9I GATC 3 cut(s) 111, 229, 600
LmnI GCTCC 2 cut(s) 36, 581
Lsp1109I GCAGC 8 cut(s) 58, 62, 162, 180, 181, 184, 202, 467
LweI GCATC 1 cut(s) 400
MaeII ACGT 1 cut(s) 141
MalI GATC 3 cut(s) 113, 231, 602
MboI GATC 3 cut(s) 111, 229, 600
MboII GAAGA 4 cut(s) 143, 436, 511, 613
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 531
MlsI TGGCCA 1 cut(s) 20
MluNI TGGCCA 1 cut(s) 20
Mly113I GGCGCC 2 cut(s) 81, 344
Mox20I TGGCCA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 20
Msp20I TGGCCA 1 cut(s) 20
MspA1I CMGCKG 5 cut(s) 49, 166, 175, 193, 281
MspI CCGG 6 cut(s) 63, 216, 233, 248, 258, 347
MspR9I CCNGG 5 cut(s) 217, 234, 242, 259, 348
MvaI CCWGG 1 cut(s) 242
MvnI CGCG 4 cut(s) 166, 281, 326, 509
NarI GGCGCC 2 cut(s) 81, 344
NciI CCSGG 4 cut(s) 217, 234, 259, 348
NdeII GATC 3 cut(s) 111, 229, 600
NlaIII CATG 2 cut(s) 493, 525
NlaIV GGNNCC 6 cut(s) 82, 220, 231, 246, 345, 577
PaeR7I CTCGAG 1 cut(s) 605
PcsI WCGNNNNNNNCGW 1 cut(s) 612
PfeI GAWTC 6 cut(s) 66, 199, 224, 470, 568, 610
Pfl23II CGTACG 1 cut(s) 596
PluTI GGCGCC 2 cut(s) 84, 347
Ppu21I YACGTR 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PspLI CGTACG 1 cut(s) 596
PspN4I GGNNCC 6 cut(s) 82, 220, 231, 246, 345, 577
PspPI GGNCC 2 cut(s) 219, 245
PsrI GAACNNNNNNTAC 2 cut(s) 126, 158
PstNI CAGNNNCTG 3 cut(s) 52, 175, 196
PsuI RGATCY 1 cut(s) 229
PvuII CAGCTG 2 cut(s) 175, 193
RsaI GTAC 2 cut(s) 144, 598
RsaNI GTAC 2 cut(s) 143, 597
SacII CCGCGG 2 cut(s) 167, 282
Sau3AI GATC 3 cut(s) 111, 229, 600
Sau96I GGNCC 2 cut(s) 219, 245
ScrFI CCNGG 5 cut(s) 217, 234, 242, 259, 348
SduI GDGCHC 1 cut(s) 531
SetI ASST 7 cut(s) 41, 144, 177, 186, 195, 276, 561
SfaNI GCATC 1 cut(s) 400
SfoI GGCGCC 2 cut(s) 82, 345
Sfr274I CTCGAG 1 cut(s) 605
Sfr303I CCGCGG 2 cut(s) 167, 282
SgrBI CCGCGG 2 cut(s) 167, 282
SinI GGWCC 2 cut(s) 219, 245
SlaI CTCGAG 1 cut(s) 605
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
SspDI GGCGCC 2 cut(s) 80, 343
StyD4I CCNGG 5 cut(s) 215, 232, 240, 257, 346
TaaI ACNGT 2 cut(s) 118, 596
TaiI ACGT 1 cut(s) 144
TaqI TCGA 5 cut(s) 120, 551, 571, 606, 615
TfiI GAWTC 6 cut(s) 66, 199, 224, 470, 568, 610
TseI GCWGC 8 cut(s) 46, 75, 169, 172, 175, 190, 193, 480
TspDTI ATGAA 3 cut(s) 144, 284, 505
TspGWI ACGGA 2 cut(s) 115, 407
VpaK11BI GGWCC 2 cut(s) 219, 245
XhoI CTCGAG 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.