pycom10g27790

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007033
Physical Location & Seq
Forward (+)
28674529 .. 28675173
645 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g27790.1

Sequence Viewer

Length: 645 bp
ATGGACGACGCGGGTGGAGCGAGGGGGGTTTTGGGCAGGCGGAGCTTCAAGCAACGGCTGAGACTGAACGGGTTCGGATGCTGTGGGGCCACCTGGGGCTTCGGACAGACGGCGGCGATTGGCGCCAGAGAGGATGAGGAAGATCGAGAACCGCAAAATCTTCCGCCACAAGAACGAGTGGTTATAACTGTGGGCCAGGCTGAACCGGTTAATATTTGGAATCCGGATTGCGTTGCTCCGGTTTCGAACGGGTCGGGTATGAATTTAGCTGCCGCTCTGGCAGCCGAGCGGCAGTTCCGGGATCCGCAGGATACGGAGGCAGAGGGGTCCGTGGGTCCCGCAGATAATGCAAGGACGGGAACAACGGCGCCGGGAACGCCGATGAGAGTGTCGCTGATGAGGCTGCTTGAGGAGACGGAGATGGACGGCTGCGACTCGTTTGGCGGTGGCGCGACGGCGGACAGTGAAAAAGCAGCGGAGGGAGGGATAGGGAATGATTCGATGTGCTGCGTGTGCATGGCAAGGAAAAAAGGCGCGGCTTTCATCCCATGTGGGCACACATTTTGCAGGGTGTGTTCGAGGGAGGTGTGGTTGAATCGAGGTTCCTGTCCCCTCTGCAACCGTTCGATCGCTCGAGATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

215

Amino Acids

22.61

Weight (kDa)

5.06

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 166 - 211 1.1e-11 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 168 - 206 2.4e-06 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 169 - 212 1.2e-06 Prokaryotic RING finger family 4
zf-C3HC4 PF00097 169 - 206 2.2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 185
AccB1I GGYRCC 2 cut(s) 122, 367
AccBSI CCGCTC 2 cut(s) 275, 289
AccII CGCG 3 cut(s) 11, 452, 536
AccIII TCCGGA 1 cut(s) 223
AclWI GGATC 3 cut(s) 296, 309, 632
AcsI RAATTY 1 cut(s) 262
AcyI GRCGYC 2 cut(s) 123, 368
AgeI ACCGGT 1 cut(s) 205
AgsI TTSAA 2 cut(s) 49, 595
AjnI CCWGG 2 cut(s) 92, 195
AluBI AGCT 2 cut(s) 45, 269
AluI AGCT 2 cut(s) 45, 269
Alw26I GTCTC 2 cut(s) 55, 407
AlwI GGATC 3 cut(s) 296, 309, 632
Ama87I CYCGRG 1 cut(s) 633
Aor13HI TCCGGA 1 cut(s) 223
AoxI GGCC 2 cut(s) 87, 193
ApeKI GCWGC 6 cut(s) 269, 281, 403, 429, 473, 507
ApoI RAATTY 1 cut(s) 262
AsiGI ACCGGT 1 cut(s) 205
Asp700I GAANNNNTTC 1 cut(s) 71
AspLEI GCGC 4 cut(s) 125, 370, 452, 536
AspS9I GGNCC 4 cut(s) 87, 193, 327, 335
AsuC2I CCSGG 2 cut(s) 299, 372
AsuII TTCGAA 1 cut(s) 245
AvaI CYCGRG 1 cut(s) 633
AvaII GGWCC 2 cut(s) 327, 335
BaeGI GKGCMC 1 cut(s) 558
BamHI GGATCC 1 cut(s) 301
BanI GGYRCC 2 cut(s) 122, 367
BbvI GCAGC 6 cut(s) 256, 293, 390, 416, 485, 494
BccI CCATC 1 cut(s) 415
BceAI ACGGC 5 cut(s) 71, 126, 381, 442, 471
BciT130I CCWGG 2 cut(s) 94, 197
BciVI GTATCC 1 cut(s) 304
BcnI CCSGG 2 cut(s) 299, 372
BcoDI GTCTC 2 cut(s) 55, 407
BfoI RGCGCY 2 cut(s) 126, 371
BfuI GTATCC 1 cut(s) 304
BglI GCCNNNNNGGC 1 cut(s) 278
Bme1390I CCNGG 4 cut(s) 94, 197, 299, 372
Bme18I GGWCC 2 cut(s) 327, 335
BmeT110I CYCGRG 1 cut(s) 633
BmgT120I GGNCC 4 cut(s) 87, 193, 327, 335
BmiI GGNNCC 8 cut(s) 88, 124, 303, 328, 336, 337, 369, 604
BmrFI CCNGG 4 cut(s) 94, 197, 299, 372
BmsI GCATC 1 cut(s) 68
Bpu14I TTCGAA 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 428
BpuMI CCSGG 2 cut(s) 299, 372
BsaHI GRCGYC 2 cut(s) 123, 368
BsaJI CCNNGG 2 cut(s) 93, 330
BsaWI WCCGGW 3 cut(s) 205, 223, 238
BsaXI ACNNNNNCTCC 2 cut(s) 575, 605
Bse118I RCCGGY 1 cut(s) 205
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 2 cut(s) 94, 197
BseDI CCNNGG 2 cut(s) 93, 330
BseGI GGATG 3 cut(s) 83, 139, 543
BseMII CTCAG 1 cut(s) 50
BseRI GAGGAG 1 cut(s) 425
BseSI GKGCMC 1 cut(s) 558
BseXI GCAGC 6 cut(s) 256, 293, 390, 416, 485, 494
Bsh1236I CGCG 3 cut(s) 11, 452, 536
Bsh1285I CGRYCG 1 cut(s) 630
BshFI GGCC 2 cut(s) 89, 195
BshNI GGYRCC 2 cut(s) 122, 367
BshTI ACCGGT 1 cut(s) 205
BsiEI CGRYCG 1 cut(s) 630
BsiHKCI CYCGRG 1 cut(s) 633
BsiSI CCGG 5 cut(s) 206, 224, 239, 298, 371
BslFI GGGAC 2 cut(s) 321, 594
BsmAI GTCTC 2 cut(s) 55, 407
BsmBI CGTCTC 1 cut(s) 407
BsmFI GGGAC 2 cut(s) 321, 594
BsnI GGCC 2 cut(s) 89, 195
BsoBI CYCGRG 1 cut(s) 633
Bsp119I TTCGAA 1 cut(s) 245
Bsp1286I GDGCHC 1 cut(s) 558
Bsp13I TCCGGA 1 cut(s) 223
Bsp143I GATC 4 cut(s) 142, 301, 627, 637
BspANI GGCC 2 cut(s) 89, 195
BspCNI CTCAG 1 cut(s) 51
BspEI TCCGGA 1 cut(s) 223
BspFNI CGCG 3 cut(s) 11, 452, 536
BspLI GGNNCC 8 cut(s) 88, 124, 303, 328, 336, 337, 369, 604
BspPI GGATC 3 cut(s) 296, 309, 632
BspT104I TTCGAA 1 cut(s) 245
BspT107I GGYRCC 2 cut(s) 122, 367
BsrBI CCGCTC 2 cut(s) 275, 289
BsrFI RCCGGY 1 cut(s) 205
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 2 cut(s) 93, 330
BssMI GATC 4 cut(s) 142, 301, 627, 637
BssNI GRCGYC 2 cut(s) 123, 368
Bst2UI CCWGG 2 cut(s) 94, 197
Bst4CI ACNGT 3 cut(s) 190, 464, 623
BstACI GRCGYC 2 cut(s) 123, 368
BstAPI GCANNNNNTGC 1 cut(s) 347
BstBI TTCGAA 1 cut(s) 245
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 1 cut(s) 59
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 3 cut(s) 83, 139, 543
BstFNI CGCG 3 cut(s) 11, 452, 536
BstH2I RGCGCY 2 cut(s) 126, 371
BstHHI GCGC 4 cut(s) 125, 370, 452, 536
BstKTI GATC 4 cut(s) 145, 304, 630, 640
BstMAI GTCTC 2 cut(s) 55, 407
BstMBI GATC 4 cut(s) 142, 301, 627, 637
BstMCI CGRYCG 1 cut(s) 630
BstMWI GCNNNNNNNGC 9 cut(s) 17, 42, 122, 278, 281, 347, 376, 400, 513
BstNI CCWGG 2 cut(s) 94, 197
BstSCI CCNGG 4 cut(s) 92, 195, 297, 370
BstSLI GKGCMC 1 cut(s) 558
BstUI CGCG 3 cut(s) 11, 452, 536
BstV1I GCAGC 6 cut(s) 256, 293, 390, 416, 485, 494
BstX2I RGATCY 2 cut(s) 301, 637
BstYI RGATCY 2 cut(s) 301, 637
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 2 cut(s) 89, 195
BtgI CCRYGG 1 cut(s) 330
BtsCI GGATG 3 cut(s) 83, 139, 543
BtsIMutI CAGTG 1 cut(s) 469
Cac8I GCNNGC 1 cut(s) 38
CfoI GCGC 4 cut(s) 125, 370, 452, 536
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 4 cut(s) 87, 193, 327, 335
CseI GACGC 1 cut(s) 17
CspAI ACCGGT 1 cut(s) 205
CviAII CATG 2 cut(s) 517, 549
DdeI CTNAG 1 cut(s) 59
DinI GGCGCC 2 cut(s) 124, 369
DpnI GATC 4 cut(s) 144, 303, 629, 639
DpnII GATC 4 cut(s) 142, 301, 627, 637
EciI GGCGGA 3 cut(s) 55, 153, 473
Eco47I GGWCC 2 cut(s) 327, 335
Eco88I CYCGRG 1 cut(s) 633
EcoO109I RGGNCCY 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 92, 195
EgeI GGCGCC 2 cut(s) 124, 369
EheI GGCGCC 2 cut(s) 124, 369
Esp3I CGTCTC 1 cut(s) 407
FaeI CATG 2 cut(s) 520, 552
FaiI YATR 4 cut(s) 185, 260, 518, 550
FaqI GGGAC 2 cut(s) 321, 594
FatI CATG 2 cut(s) 516, 548
FauI CCCGC 2 cut(s) 4, 346
FokI GGATG 3 cut(s) 90, 146, 530
GlaI GCGC 4 cut(s) 124, 369, 451, 535
HaeII RGCGCY 2 cut(s) 126, 371
HaeIII GGCC 2 cut(s) 89, 195
HapII CCGG 5 cut(s) 206, 224, 239, 298, 371
HgaI GACGC 1 cut(s) 17
HhaI GCGC 4 cut(s) 125, 370, 452, 536
Hin1I GRCGYC 2 cut(s) 123, 368
Hin1II CATG 2 cut(s) 520, 552
Hin6I GCGC 4 cut(s) 123, 368, 450, 534
HinP1I GCGC 4 cut(s) 123, 368, 450, 534
HinfI GANTC 4 cut(s) 220, 434, 497, 595
HpaII CCGG 5 cut(s) 206, 224, 239, 298, 371
Hpy188I TCNGA 2 cut(s) 77, 104
Hpy188III TCNNGA 3 cut(s) 146, 224, 635
Hpy99I CGWCG 2 cut(s) 11, 457
HpyCH4III ACNGT 3 cut(s) 190, 464, 623
HpyCH4V TGCA 4 cut(s) 350, 516, 567, 618
HpyF10VI GCNNNNNNNGC 9 cut(s) 17, 42, 122, 278, 281, 347, 376, 400, 513
HpyF3I CTNAG 1 cut(s) 59
Hsp92I GRCGYC 2 cut(s) 123, 368
Hsp92II CATG 2 cut(s) 520, 552
HspAI GCGC 4 cut(s) 123, 368, 450, 534
KasI GGCGCC 2 cut(s) 122, 367
KflI GGGWCCC 1 cut(s) 335
Kpn2I TCCGGA 1 cut(s) 223
Kzo9I GATC 4 cut(s) 142, 301, 627, 637
LmnI GCTCC 3 cut(s) 17, 42, 241
Lsp1109I GCAGC 6 cut(s) 256, 293, 390, 416, 485, 494
LweI GCATC 1 cut(s) 68
MalI GATC 4 cut(s) 144, 303, 629, 639
MbiI CCGCTC 2 cut(s) 275, 289
MboI GATC 4 cut(s) 142, 301, 627, 637
MboII GAAGA 2 cut(s) 152, 152
MflI RGATCY 2 cut(s) 301, 637
MhlI GDGCHC 1 cut(s) 558
MluCI AATT 1 cut(s) 262
Mly113I GGCGCC 2 cut(s) 123, 368
MlyI GAGTC 1 cut(s) 428
MroI TCCGGA 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 1 cut(s) 210
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 5 cut(s) 206, 224, 239, 298, 371
MspR9I CCNGG 4 cut(s) 94, 197, 299, 372
MvaI CCWGG 2 cut(s) 94, 197
MvnI CGCG 3 cut(s) 11, 452, 536
MwoI GCNNNNNNNGC 9 cut(s) 17, 42, 122, 278, 281, 347, 376, 400, 513
NarI GGCGCC 2 cut(s) 123, 368
NciI CCSGG 2 cut(s) 299, 372
NdeII GATC 4 cut(s) 142, 301, 627, 637
NlaIII CATG 2 cut(s) 520, 552
NlaIV GGNNCC 8 cut(s) 88, 124, 303, 328, 336, 337, 369, 604
NmeAIII GCCGAG 1 cut(s) 310
NspV TTCGAA 1 cut(s) 245
PaeR7I CTCGAG 1 cut(s) 633
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 3 cut(s) 220, 497, 595
PfoI TCCNGGA 1 cut(s) 297
PinAI ACCGGT 1 cut(s) 205
Ple19I CGATCG 1 cut(s) 630
PleI GAGTC 1 cut(s) 428
PluTI GGCGCC 2 cut(s) 126, 371
PpsI GAGTC 1 cut(s) 428
PpuMI RGGWCCY 1 cut(s) 335
PsiI TTATAA 1 cut(s) 185
Psp5II RGGWCCY 1 cut(s) 335
Psp6I CCWGG 2 cut(s) 92, 195
PspGI CCWGG 2 cut(s) 92, 195
PspN4I GGNNCC 8 cut(s) 88, 124, 303, 328, 336, 337, 369, 604
PspPI GGNCC 4 cut(s) 87, 193, 327, 335
PspPPI RGGWCCY 1 cut(s) 335
PsuI RGATCY 2 cut(s) 301, 637
PvuI CGATCG 1 cut(s) 630
SaqAI TTAA 1 cut(s) 210
Sau3AI GATC 4 cut(s) 142, 301, 627, 637
Sau96I GGNCC 4 cut(s) 87, 193, 327, 335
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 4 cut(s) 94, 197, 299, 372
SduI GDGCHC 1 cut(s) 558
SetI ASST 5 cut(s) 47, 95, 271, 588, 604
SfaNI GCATC 1 cut(s) 68
SfoI GGCGCC 2 cut(s) 124, 369
Sfr274I CTCGAG 1 cut(s) 633
SfuI TTCGAA 1 cut(s) 245
SinI GGWCC 2 cut(s) 327, 335
SlaI CTCGAG 1 cut(s) 633
SmlI CTYRAG 2 cut(s) 407, 633
SmoI CTYRAG 2 cut(s) 407, 633
Sse9I AATT 1 cut(s) 262
SspDI GGCGCC 2 cut(s) 122, 367
SspI AATATT 1 cut(s) 214
StyD4I CCNGG 4 cut(s) 92, 195, 297, 370
TaaI ACNGT 3 cut(s) 190, 464, 623
TaqI TCGA 7 cut(s) 145, 245, 500, 578, 598, 626, 634
TasI AATT 1 cut(s) 262
TauI GCSGC 4 cut(s) 116, 275, 292, 539
TfiI GAWTC 3 cut(s) 220, 497, 595
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 1 cut(s) 469
TseI GCWGC 6 cut(s) 269, 281, 403, 429, 473, 507
TspDTI ATGAA 2 cut(s) 275, 532
TspGWI ACGGA 3 cut(s) 319, 329, 431
TspRI CASTG 1 cut(s) 469
VpaK11BI GGWCC 2 cut(s) 327, 335
XapI RAATTY 1 cut(s) 262
XhoI CTCGAG 1 cut(s) 633
XmnI GAANNNNTTC 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.