MD05G1353900.v1.1

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007033
Physical Location & Seq
Forward (+)
47049711 .. 47050448
738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1353900.v1.1.491

Sequence Viewer

Length: 738 bp
ATGAGTCAACTAAGTCTGATATTGCAGGAAGCGGTGCGGAGGGAGAGGGCGGCAAGGACGGTCCTCGAGGTTCTGAGAGAGCAAATGGACGACGCGGATGGAGCGAGGGGAGTTGTGGGCAGGCGGAGCTTGAAGCAACGGCTGAGACTGAGCGTGTTCGGCTGCTGTGGGGCCACCTGGGGATTCGGACGGACGTCGGCGATTGGCGCCAGAGAGGATGAGGAAGATCAAGAACCGCAAAATCTTCCGCCACAAGAACGAGTGGTTATAACTGTGGGCCAGGCTGGACCGGTTAATATTTGGGATCCGGGTTGCGTGGCTCCGGTTTCGCACGGATCGGGTATGAATTTGGCTGCCGCGCTGGCAGCCGAGCGACAGTTGCGGGCTCCGCAGGATCCGGAGGGAGAGGGGTCTGTGGGTCCCACGGGTAATGCAATCGCGGGAACAACAGCGCCGGGAACGCCGATGAGAGTGTCGCTGATGAGGCTGATTGAGGAGACGGAGATGGACGGGCGCGACGAGTTTGGCGGTGGCGCGACGGCGTTGAGCGAAAAAACAGCGGAGGGAAATATAGGGAGCGATTCGGTGTGCTGCGTGTGCATGGGAAGGAAAAAAGGTGCGGCTTTCATCCCGTGTGGGCACACATTTTGTAGGGTGTGTTCCAGAGAGGTGTGGTTGAATCGAGGCTCCTGTCCCCTCTGCAACCGTTCGATCCTCGAGATCCTCGATATATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

246

Amino Acids

26.14

Weight (kDa)

5.41

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 194 - 239 3e-11 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 197 - 234 1.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 197 - 234 1.7e-06 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 197 - 238 9.1e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 269
AatII GACGTC 1 cut(s) 197
AbsI CCTCGAGG 1 cut(s) 65
AccB1I GGYRCC 1 cut(s) 206
AccII CGCG 5 cut(s) 95, 359, 440, 516, 536
AccIII TCCGGA 1 cut(s) 397
AclWI GGATC 7 cut(s) 299, 312, 343, 389, 402, 706, 715
AcsI RAATTY 1 cut(s) 346
AcyI GRCGYC 2 cut(s) 194, 207
AgeI ACCGGT 1 cut(s) 289
AgsI TTSAA 2 cut(s) 133, 679
AjnI CCWGG 2 cut(s) 176, 279
AluBI AGCT 1 cut(s) 129
AluI AGCT 1 cut(s) 129
Alw26I GTCTC 2 cut(s) 139, 491
AlwI GGATC 7 cut(s) 299, 312, 343, 389, 402, 706, 715
Ama87I CYCGRG 2 cut(s) 65, 716
Aor13HI TCCGGA 1 cut(s) 397
AoxI GGCC 2 cut(s) 171, 277
ApeKI GCWGC 4 cut(s) 162, 353, 365, 591
ApoI RAATTY 1 cut(s) 346
AsiGI ACCGGT 1 cut(s) 289
AspLEI GCGC 5 cut(s) 209, 361, 454, 516, 536
AspS9I GGNCC 5 cut(s) 61, 171, 277, 287, 419
AsuC2I CCSGG 2 cut(s) 309, 456
AvaI CYCGRG 2 cut(s) 65, 716
AvaII GGWCC 3 cut(s) 61, 287, 419
BaeGI GKGCMC 1 cut(s) 642
BamHI GGATCC 2 cut(s) 304, 394
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 1 cut(s) 388
BbvI GCAGC 4 cut(s) 149, 340, 377, 578
BccI CCATC 2 cut(s) 92, 499
BceAI ACGGC 2 cut(s) 155, 555
BciT130I CCWGG 2 cut(s) 178, 281
BcnI CCSGG 2 cut(s) 309, 456
BcoDI GTCTC 2 cut(s) 139, 491
BfaI CTAG 1 cut(s) 736
BfoI RGCGCY 2 cut(s) 210, 455
BglI GCCNNNNNGGC 1 cut(s) 362
BisI GCNGC 7 cut(s) 51, 163, 354, 357, 366, 592, 621
BlsI GCNGC 7 cut(s) 52, 164, 355, 358, 367, 593, 622
Bme1390I CCNGG 4 cut(s) 178, 281, 309, 456
Bme18I GGWCC 3 cut(s) 61, 287, 419
BmeT110I CYCGRG 2 cut(s) 65, 716
BmgT120I GGNCC 5 cut(s) 61, 171, 277, 287, 419
BmiI GGNNCC 9 cut(s) 172, 208, 306, 321, 387, 396, 420, 421, 688
BmrFI CCNGG 4 cut(s) 178, 281, 309, 456
BoxI GACNNNNGTC 1 cut(s) 193
BpuMI CCSGG 2 cut(s) 309, 456
BsaHI GRCGYC 2 cut(s) 194, 207
BsaJI CCNNGG 2 cut(s) 177, 423
BsaWI WCCGGW 3 cut(s) 289, 322, 397
Bse118I RCCGGY 1 cut(s) 289
BseAI TCCGGA 1 cut(s) 397
BseBI CCWGG 2 cut(s) 178, 281
BseDI CCNNGG 2 cut(s) 177, 423
BseGI GGATG 3 cut(s) 103, 223, 627
BseMII CTCAG 3 cut(s) 65, 134, 140
BseRI GAGGAG 1 cut(s) 509
BseSI GKGCMC 1 cut(s) 642
BseXI GCAGC 4 cut(s) 149, 340, 377, 578
Bsh1236I CGCG 5 cut(s) 95, 359, 440, 516, 536
BshFI GGCC 2 cut(s) 173, 279
BshNI GGYRCC 1 cut(s) 206
BshTI ACCGGT 1 cut(s) 289
BsiHKCI CYCGRG 2 cut(s) 65, 716
BsiSI CCGG 5 cut(s) 290, 308, 323, 398, 455
BslFI GGGAC 2 cut(s) 405, 678
BsmAI GTCTC 2 cut(s) 139, 491
BsmBI CGTCTC 1 cut(s) 491
BsmFI GGGAC 2 cut(s) 405, 678
BsnI GGCC 2 cut(s) 173, 279
BsoBI CYCGRG 2 cut(s) 65, 716
Bsp1286I GDGCHC 2 cut(s) 388, 642
Bsp13I TCCGGA 1 cut(s) 397
Bsp143I GATC 6 cut(s) 226, 304, 335, 394, 711, 720
BspANI GGCC 2 cut(s) 173, 279
BspCNI CTCAG 3 cut(s) 66, 135, 141
BspEI TCCGGA 1 cut(s) 397
BspFNI CGCG 5 cut(s) 95, 359, 440, 516, 536
BspLI GGNNCC 9 cut(s) 172, 208, 306, 321, 387, 396, 420, 421, 688
BspPI GGATC 7 cut(s) 299, 312, 343, 389, 402, 706, 715
BspT107I GGYRCC 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 289
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 2 cut(s) 177, 423
BssMI GATC 6 cut(s) 226, 304, 335, 394, 711, 720
BssNI GRCGYC 2 cut(s) 194, 207
Bst2UI CCWGG 2 cut(s) 178, 281
Bst4CI ACNGT 4 cut(s) 61, 274, 378, 707
BstACI GRCGYC 2 cut(s) 194, 207
BstC8I GCNNGC 3 cut(s) 122, 363, 384
BstDEI CTNAG 4 cut(s) 11, 74, 143, 149
BstDSI CCRYGG 1 cut(s) 423
BstF5I GGATG 3 cut(s) 103, 223, 627
BstFNI CGCG 5 cut(s) 95, 359, 440, 516, 536
BstH2I RGCGCY 2 cut(s) 210, 455
BstHHI GCGC 5 cut(s) 209, 361, 454, 516, 536
BstKTI GATC 6 cut(s) 229, 307, 338, 397, 714, 723
BstMAI GTCTC 2 cut(s) 139, 491
BstMBI GATC 6 cut(s) 226, 304, 335, 394, 711, 720
BstNI CCWGG 2 cut(s) 178, 281
BstPAI GACNNNNGTC 1 cut(s) 193
BstSCI CCNGG 4 cut(s) 176, 279, 307, 454
BstSLI GKGCMC 1 cut(s) 642
BstUI CGCG 5 cut(s) 95, 359, 440, 516, 536
BstV1I GCAGC 4 cut(s) 149, 340, 377, 578
BstX2I RGATCY 3 cut(s) 304, 394, 720
BstYI RGATCY 3 cut(s) 304, 394, 720
BsuRI GGCC 2 cut(s) 173, 279
BtgI CCRYGG 1 cut(s) 423
BtsCI GGATG 3 cut(s) 103, 223, 627
Cac8I GCNNGC 3 cut(s) 122, 363, 384
CfoI GCGC 5 cut(s) 209, 361, 454, 516, 536
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 5 cut(s) 61, 171, 277, 287, 419
CseI GACGC 1 cut(s) 101
CspAI ACCGGT 1 cut(s) 289
CviAII CATG 1 cut(s) 601
DdeI CTNAG 4 cut(s) 11, 74, 143, 149
DinI GGCGCC 1 cut(s) 208
DpnI GATC 6 cut(s) 228, 306, 337, 396, 713, 722
DpnII GATC 6 cut(s) 226, 304, 335, 394, 711, 720
EciI GGCGGA 2 cut(s) 139, 237
Eco24I GRGCYC 1 cut(s) 388
Eco47I GGWCC 3 cut(s) 61, 287, 419
Eco88I CYCGRG 2 cut(s) 65, 716
EcoO109I RGGNCCY 1 cut(s) 419
EcoRII CCWGG 2 cut(s) 176, 279
EcoT38I GRGCYC 1 cut(s) 388
EgeI GGCGCC 1 cut(s) 208
EheI GGCGCC 1 cut(s) 208
Esp3I CGTCTC 1 cut(s) 491
FaeI CATG 1 cut(s) 604
FaiI YATR 5 cut(s) 269, 344, 572, 602, 731
FaqI GGGAC 2 cut(s) 405, 678
FatI CATG 1 cut(s) 600
FauI CCCGC 2 cut(s) 375, 433
Fnu4HI GCNGC 7 cut(s) 51, 163, 354, 357, 366, 592, 621
FokI GGATG 3 cut(s) 110, 230, 614
FriOI GRGCYC 1 cut(s) 388
Fsp4HI GCNGC 7 cut(s) 51, 163, 354, 357, 366, 592, 621
FspBI CTAG 1 cut(s) 736
GlaI GCGC 5 cut(s) 208, 360, 453, 515, 535
GluI GCNGC 7 cut(s) 51, 163, 354, 357, 366, 592, 621
HaeII RGCGCY 2 cut(s) 210, 455
HaeIII GGCC 2 cut(s) 173, 279
HapII CCGG 5 cut(s) 290, 308, 323, 398, 455
HgaI GACGC 1 cut(s) 101
HhaI GCGC 5 cut(s) 209, 361, 454, 516, 536
Hin1I GRCGYC 2 cut(s) 194, 207
Hin1II CATG 1 cut(s) 604
Hin6I GCGC 5 cut(s) 207, 359, 452, 514, 534
HinP1I GCGC 5 cut(s) 207, 359, 452, 514, 534
HincII GTYRAC 1 cut(s) 8
HindII GTYRAC 1 cut(s) 8
HinfI GANTC 4 cut(s) 4, 183, 581, 679
HpaII CCGG 5 cut(s) 290, 308, 323, 398, 455
Hpy166II GTNNAC 1 cut(s) 8
Hpy188I TCNGA 3 cut(s) 18, 75, 188
Hpy188III TCNNGA 4 cut(s) 230, 398, 663, 718
Hpy8I GTNNAC 1 cut(s) 8
Hpy99I CGWCG 4 cut(s) 95, 199, 521, 541
HpyAV CCTTC 1 cut(s) 600
HpyCH4III ACNGT 4 cut(s) 61, 274, 378, 707
HpyCH4IV ACGT 1 cut(s) 194
HpyCH4V TGCA 4 cut(s) 25, 434, 600, 702
HpyF3I CTNAG 4 cut(s) 11, 74, 143, 149
HpySE526I ACGT 1 cut(s) 194
Hsp92I GRCGYC 2 cut(s) 194, 207
Hsp92II CATG 1 cut(s) 604
HspAI GCGC 5 cut(s) 207, 359, 452, 514, 534
KasI GGCGCC 1 cut(s) 206
KflI GGGWCCC 1 cut(s) 419
Kpn2I TCCGGA 1 cut(s) 397
Kzo9I GATC 6 cut(s) 226, 304, 335, 394, 711, 720
LmnI GCTCC 6 cut(s) 101, 126, 325, 391, 576, 692
Lsp1109I GCAGC 4 cut(s) 149, 340, 377, 578
MaeI CTAG 1 cut(s) 736
MaeII ACGT 1 cut(s) 194
MalI GATC 6 cut(s) 228, 306, 337, 396, 713, 722
MboI GATC 6 cut(s) 226, 304, 335, 394, 711, 720
MboII GAAGA 2 cut(s) 236, 236
MflI RGATCY 3 cut(s) 304, 394, 720
MhlI GDGCHC 2 cut(s) 388, 642
MluCI AATT 1 cut(s) 346
Mly113I GGCGCC 1 cut(s) 207
MlyI GAGTC 1 cut(s) 13
MroI TCCGGA 1 cut(s) 397
MseI TTAA 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 560
MspI CCGG 5 cut(s) 290, 308, 323, 398, 455
MspR9I CCNGG 4 cut(s) 178, 281, 309, 456
MvaI CCWGG 2 cut(s) 178, 281
MvnI CGCG 5 cut(s) 95, 359, 440, 516, 536
NarI GGCGCC 1 cut(s) 207
NciI CCSGG 2 cut(s) 309, 456
NdeII GATC 6 cut(s) 226, 304, 335, 394, 711, 720
NlaIII CATG 1 cut(s) 604
NlaIV GGNNCC 9 cut(s) 172, 208, 306, 321, 387, 396, 420, 421, 688
NmeAIII GCCGAG 1 cut(s) 394
PaeR7I CTCGAG 2 cut(s) 65, 716
PcsI WCGNNNNNNNCGW 2 cut(s) 516, 723
PfeI GAWTC 3 cut(s) 183, 581, 679
PinAI ACCGGT 1 cut(s) 289
PkrI GCNGC 7 cut(s) 52, 164, 355, 358, 367, 593, 622
PleI GAGTC 1 cut(s) 12
PluTI GGCGCC 1 cut(s) 210
PpsI GAGTC 1 cut(s) 12
PpuMI RGGWCCY 1 cut(s) 419
PshAI GACNNNNGTC 1 cut(s) 193
PsiI TTATAA 1 cut(s) 269
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 2 cut(s) 176, 279
PspGI CCWGG 2 cut(s) 176, 279
PspN4I GGNNCC 9 cut(s) 172, 208, 306, 321, 387, 396, 420, 421, 688
PspPI GGNCC 5 cut(s) 61, 171, 277, 287, 419
PspPPI RGGWCCY 1 cut(s) 419
PspXI VCTCGAGB 1 cut(s) 65
PsrI GAACNNNNNNTAC 2 cut(s) 643, 675
PsuI RGATCY 3 cut(s) 304, 394, 720
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 7 cut(s) 51, 163, 354, 357, 366, 592, 621
Sau3AI GATC 6 cut(s) 226, 304, 335, 394, 711, 720
Sau96I GGNCC 5 cut(s) 61, 171, 277, 287, 419
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 4 cut(s) 178, 281, 309, 456
SduI GDGCHC 2 cut(s) 388, 642
SetI ASST 6 cut(s) 72, 131, 179, 197, 619, 672
SfoI GGCGCC 1 cut(s) 208
Sfr274I CTCGAG 2 cut(s) 65, 716
SinI GGWCC 3 cut(s) 61, 287, 419
SlaI CTCGAG 2 cut(s) 65, 716
SmlI CTYRAG 2 cut(s) 65, 716
SmoI CTYRAG 2 cut(s) 65, 716
Sse9I AATT 1 cut(s) 346
SspDI GGCGCC 1 cut(s) 206
SspI AATATT 1 cut(s) 298
SspMI CTAG 1 cut(s) 736
StyD4I CCNGG 4 cut(s) 176, 279, 307, 454
TaaI ACNGT 4 cut(s) 61, 274, 378, 707
TaiI ACGT 1 cut(s) 197
TaqI TCGA 5 cut(s) 66, 682, 710, 717, 726
TasI AATT 1 cut(s) 346
TauI GCSGC 3 cut(s) 53, 359, 623
TfiI GAWTC 3 cut(s) 183, 581, 679
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TseI GCWGC 4 cut(s) 162, 353, 365, 591
TspDTI ATGAA 2 cut(s) 359, 616
TspGWI ACGGA 3 cut(s) 205, 348, 515
VpaK11BI GGWCC 3 cut(s) 61, 287, 419
XapI RAATTY 1 cut(s) 346
XhoI CTCGAG 2 cut(s) 65, 716
XspI CTAG 1 cut(s) 736
ZraI GACGTC 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.