Rh2DG441100

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007033
Physical Location & Seq
Forward (+)
64095520 .. 64120996
25477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG441100.1

Sequence Viewer

Length: 456 bp
ATGAGTCAACTCAGTCTGGTGTTGCAGGAAGCGGTCCGGAGAGGAAGGGAGGCCAAGATGATTCTTGAGGTTCTGAGGGAGCAAATGGACGATGGCGATGCAGCCAAGGCAGGGAGGAGGAGTTTCAAGCAGCGGCTGAGATTGACCGGAATCGGGTGCTGCGGCGCCGCCTGGGACAATGAGTTGGTCCAGTTGAAGCATGAAGCAAGGCTCAAAGGAGGATTCTATGTCGAGCTAGACTTGAAGATTTTTGTTCCAATGACAGTCCTAGCACTTCTTATTCTTATTCCAGTCAATGTATCGAGTGGTACCTTATTTTTCTTAAAGAAAGAGTTGGTTTTGAGTGACATTGATAGGCTCTCTATATCAAATGTGCGCCCCAAGTCAATAAGGTTGGTTACTTACAAGAAAAGAACAAAGCCTCAAAGTGCATCGATGAAATTCATTACAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

151

Amino Acids

17.05

Weight (kDa)

9.95

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 79 - 134 5.8e-07 Late exocytosis, associated with Golgi transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 308
AccB1I GGYRCC 2 cut(s) 164, 308
AccIII TCCGGA 1 cut(s) 36
AciI CCGC 4 cut(s) 32, 133, 162, 168
AcsI RAATTY 1 cut(s) 440
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 1 cut(s) 310
AfiI CCNNNNNNNGG 2 cut(s) 111, 153
AgsI TTSAA 3 cut(s) 127, 196, 244
AjnI CCWGG 1 cut(s) 170
AluBI AGCT 1 cut(s) 235
AluI AGCT 1 cut(s) 235
AlwNI CAGNNNCTG 1 cut(s) 136
Aor13HI TCCGGA 1 cut(s) 36
AoxI GGCC 1 cut(s) 51
ApeKI GCWGC 3 cut(s) 101, 130, 159
ApoI RAATTY 1 cut(s) 440
Asp718I GGTACC 1 cut(s) 308
AspLEI GCGC 2 cut(s) 167, 378
AspS9I GGNCC 2 cut(s) 34, 187
AvaII GGWCC 2 cut(s) 34, 187
BanI GGYRCC 2 cut(s) 164, 308
BbvI GCAGC 3 cut(s) 113, 142, 146
BccI CCATC 1 cut(s) 86
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BciT130I CCWGG 1 cut(s) 172
BfaI CTAG 2 cut(s) 236, 269
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 6 cut(s) 102, 131, 134, 160, 163, 168
BlsI GCNGC 6 cut(s) 103, 132, 135, 161, 164, 169
Bme1390I CCNGG 1 cut(s) 172
Bme18I GGWCC 2 cut(s) 34, 187
BmgT120I GGNCC 2 cut(s) 34, 187
BmiI GGNNCC 2 cut(s) 166, 310
BmrFI CCNGG 1 cut(s) 172
BmsI GCATC 2 cut(s) 88, 440
BpuEI CTTGAG 1 cut(s) 86
Bsa29I ATCGAT 1 cut(s) 434
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 2 cut(s) 105, 171
BsaWI WCCGGW 2 cut(s) 36, 146
BsaXI ACNNNNNCTCC 2 cut(s) 106, 136
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 153
Bse1I ACTGG 2 cut(s) 190, 290
BseAI TCCGGA 1 cut(s) 36
BseBI CCWGG 1 cut(s) 172
BseCI ATCGAT 1 cut(s) 434
BseDI CCNNGG 2 cut(s) 105, 171
BseLI CCNNNNNNNGG 2 cut(s) 111, 153
BseMII CTCAG 3 cut(s) 25, 65, 128
BseNI ACTGG 2 cut(s) 190, 290
BseRI GAGGAG 2 cut(s) 130, 133
BseXI GCAGC 3 cut(s) 113, 142, 146
BshFI GGCC 1 cut(s) 53
BshNI GGYRCC 2 cut(s) 164, 308
BshVI ATCGAT 1 cut(s) 434
BsiSI CCGG 2 cut(s) 37, 147
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 2 cut(s) 111, 153
BsmFI GGGAC 1 cut(s) 188
BsnI GGCC 1 cut(s) 53
Bsp13I TCCGGA 1 cut(s) 36
BspACI CCGC 4 cut(s) 32, 133, 162, 168
BspANI GGCC 1 cut(s) 53
BspCNI CTCAG 3 cut(s) 24, 66, 129
BspDI ATCGAT 1 cut(s) 434
BspEI TCCGGA 1 cut(s) 36
BspLI GGNNCC 2 cut(s) 166, 310
BspT107I GGYRCC 2 cut(s) 164, 308
BsrI ACTGG 2 cut(s) 190, 290
BssECI CCNNGG 2 cut(s) 105, 171
BssNI GRCGYC 1 cut(s) 165
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 1 cut(s) 172
Bst4CI ACNGT 1 cut(s) 265
BstACI GRCGYC 1 cut(s) 165
BstDEI CTNAG 3 cut(s) 11, 74, 137
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 2 cut(s) 167, 378
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNI CCWGG 1 cut(s) 172
BstSCI CCNGG 1 cut(s) 170
BstV1I GCAGC 3 cut(s) 113, 142, 146
Bsu15I ATCGAT 1 cut(s) 434
BsuRI GGCC 1 cut(s) 53
BsuTUI ATCGAT 1 cut(s) 434
BtgZI GCGATG 1 cut(s) 111
CaiI CAGNNNCTG 1 cut(s) 136
CfoI GCGC 2 cut(s) 167, 378
Cfr13I GGNCC 2 cut(s) 34, 187
ClaI ATCGAT 1 cut(s) 434
CpoI CGGWCCG 1 cut(s) 34
Csp6I GTAC 1 cut(s) 309
CspI CGGWCCG 1 cut(s) 34
CviAII CATG 1 cut(s) 200
CviJI RGCY 7 cut(s) 53, 104, 136, 211, 235, 358, 421
CviKI_1 RGCY 7 cut(s) 53, 104, 136, 211, 235, 358, 421
CviQI GTAC 1 cut(s) 309
DdeI CTNAG 3 cut(s) 11, 74, 137
DinI GGCGCC 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 105
Eco47I GGWCC 2 cut(s) 34, 187
EcoRII CCWGG 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 105
EgeI GGCGCC 1 cut(s) 166
EheI GGCGCC 1 cut(s) 166
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 1 cut(s) 203
FaiI YATR 3 cut(s) 201, 228, 365
FaqI GGGAC 1 cut(s) 188
FatI CATG 1 cut(s) 199
Fnu4HI GCNGC 6 cut(s) 102, 131, 134, 160, 163, 168
Fsp4HI GCNGC 6 cut(s) 102, 131, 134, 160, 163, 168
FspBI CTAG 2 cut(s) 236, 269
GlaI GCGC 2 cut(s) 166, 377
GluI GCNGC 6 cut(s) 102, 131, 134, 160, 163, 168
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 1 cut(s) 53
HapII CCGG 2 cut(s) 37, 147
HhaI GCGC 2 cut(s) 167, 378
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 1 cut(s) 203
Hin6I GCGC 2 cut(s) 165, 376
HinP1I GCGC 2 cut(s) 165, 376
HincII GTYRAC 1 cut(s) 8
HindII GTYRAC 1 cut(s) 8
HinfI GANTC 4 cut(s) 4, 61, 150, 222
HpaII CCGG 2 cut(s) 37, 147
Hpy166II GTNNAC 1 cut(s) 8
Hpy188I TCNGA 1 cut(s) 75
Hpy188III TCNNGA 2 cut(s) 37, 65
Hpy8I GTNNAC 1 cut(s) 8
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4V TGCA 3 cut(s) 25, 101, 431
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 3 cut(s) 11, 74, 137
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 1 cut(s) 203
HspAI GCGC 2 cut(s) 165, 376
KasI GGCGCC 1 cut(s) 164
Kpn2I TCCGGA 1 cut(s) 36
KpnI GGTACC 1 cut(s) 312
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 9 cut(s) 2, 11, 50, 96, 157, 160, 184, 203, 303
Lsp1109I GCAGC 3 cut(s) 113, 142, 146
LweI GCATC 2 cut(s) 88, 440
MaeI CTAG 2 cut(s) 236, 269
MaeIII GTNAC 2 cut(s) 344, 397
MboII GAAGA 1 cut(s) 256
MluCI AATT 1 cut(s) 440
Mly113I GGCGCC 1 cut(s) 165
MlyI GAGTC 1 cut(s) 13
MnlI CCTC 8 cut(s) 35, 43, 61, 69, 108, 111, 212, 432
MroI TCCGGA 1 cut(s) 36
MseI TTAA 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 133
MspI CCGG 2 cut(s) 37, 147
MspR9I CCNGG 1 cut(s) 172
MvaI CCWGG 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 107
NarI GGCGCC 1 cut(s) 165
NlaIII CATG 1 cut(s) 203
NlaIV GGNNCC 2 cut(s) 166, 310
NmuCI GTSAC 1 cut(s) 344
PfeI GAWTC 3 cut(s) 61, 150, 222
PkrI GCNGC 6 cut(s) 103, 132, 135, 161, 164, 169
PleI GAGTC 1 cut(s) 12
PluTI GGCGCC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 170
PspGI CCWGG 1 cut(s) 170
PspN4I GGNNCC 2 cut(s) 166, 310
PspPI GGNCC 2 cut(s) 34, 187
PstNI CAGNNNCTG 1 cut(s) 136
RsaI GTAC 1 cut(s) 310
RsaNI GTAC 1 cut(s) 309
Rsr2I CGGWCCG 1 cut(s) 34
RsrII CGGWCCG 1 cut(s) 34
SaqAI TTAA 1 cut(s) 323
SatI GCNGC 6 cut(s) 102, 131, 134, 160, 163, 168
Sau96I GGNCC 2 cut(s) 34, 187
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 1 cut(s) 172
SetI ASST 4 cut(s) 72, 237, 314, 395
SfaNI GCATC 2 cut(s) 88, 440
SfoI GGCGCC 1 cut(s) 166
SinI GGWCC 2 cut(s) 34, 187
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
Sse9I AATT 1 cut(s) 440
SsiI CCGC 4 cut(s) 32, 133, 162, 168
SspDI GGCGCC 1 cut(s) 164
SspMI CTAG 2 cut(s) 236, 269
StyD4I CCNGG 1 cut(s) 170
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 1 cut(s) 265
TaqI TCGA 3 cut(s) 231, 302, 434
TasI AATT 1 cut(s) 440
TauI GCSGC 3 cut(s) 136, 165, 170
TfiI GAWTC 3 cut(s) 61, 150, 222
Tru1I TTAA 1 cut(s) 323
Tru9I TTAA 1 cut(s) 323
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 3 cut(s) 101, 130, 159
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 3 cut(s) 216, 433, 452
VpaK11BI GGWCC 2 cut(s) 34, 187
XapI RAATTY 1 cut(s) 440
XspI CTAG 2 cut(s) 236, 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.