Rw5G001420

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007033
Physical Location & Seq
Reverse (-)
1152189 .. 1153616
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G001420.1

Sequence Viewer

Length: 711 bp
ATGAGTCAACTCAGTCTGGTGTTGCAGGAAACGGTCCGGAGAGGAAGGGAGGCCAGGACGATCCTCGAGGTTCTGAGAGAGCAAATGGACGACGGCGATGCGGCGACGGCCGGGAGGAGGAGCTTCAAGCAGCGGCTGAGATTGACCGGAATCGGGTGCTGCGGCGCCGTCTGGGGATTTGGACGGACGGCCACGATTACCGTCGAGGACGACGAAGAACAACACCTACATCCGCCGCAACAACAACCGCGGCAGCAGCTGCCGAACCTATCGCAGCTGCTGATTCTGGGGCAGGCGGCCGGGTCCGATTCGGATCCGGGTTGCCTGGGTCCGGTCAACCCCGGGTCGGGGATGAACCTCGCCGCGGCTCTGGCGGCGGAACGGCATTATCGGGAGGCGGACGAGGCCGCGTCGGGGGGTGGGGCGACGCCGGGGACGCCGATGAGGGTGTCGCTGATGAGATTGCTGGAGGAGACGGAAATGGACGGGTGCGATGCGGCGGAGGAGAAAAATGAGAAAGCGGCGGCGGCGGCGACGGGGGTGGTGGGTTGTGATTCGGTGTGCTGCGTGTGCATGGGGAGGAAGAAAGGCGCGGCGTTCATACCATGTGGGCACACTTTTTGCAGGGTGTGTTCGAGGGAGGTGTGGCTGAATCGAGGCTCCTGTCCCCTCTGCAACCGTACGATCCTCGAGATTCTCGATATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

236

Amino Acids

25.29

Weight (kDa)

5.48

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 186 - 230 5.7e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 188 - 225 1.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 188 - 225 1.6e-06 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 188 - 229 7.2e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 65
AccB1I GGYRCC 1 cut(s) 164
AccII CGCG 4 cut(s) 250, 365, 410, 593
AccIII TCCGGA 1 cut(s) 36
AclWI GGATC 4 cut(s) 55, 308, 321, 679
AcoI YGGCCR 3 cut(s) 108, 189, 297
AcyI GRCGYC 3 cut(s) 165, 428, 437
AfaI GTAC 1 cut(s) 682
AfiI CCNNNNNNNGG 8 cut(s) 117, 153, 331, 346, 347, 348, 364, 414
AgsI TTSAA 1 cut(s) 127
AjnI CCWGG 2 cut(s) 53, 324
AluBI AGCT 3 cut(s) 123, 259, 277
AluI AGCT 3 cut(s) 123, 259, 277
Alw26I GTCTC 1 cut(s) 467
AlwI GGATC 4 cut(s) 55, 308, 321, 679
AlwNI CAGNNNCTG 3 cut(s) 136, 259, 280
Ama87I CYCGRG 3 cut(s) 65, 341, 689
Aor13HI TCCGGA 1 cut(s) 36
AoxI GGCC 5 cut(s) 51, 108, 189, 297, 405
ApeKI GCWGC 8 cut(s) 130, 159, 253, 256, 259, 274, 277, 564
AspLEI GCGC 2 cut(s) 167, 593
AspS9I GGNCC 3 cut(s) 34, 303, 329
AsuC2I CCSGG 6 cut(s) 112, 301, 318, 342, 343, 432
AvaI CYCGRG 3 cut(s) 65, 341, 689
AvaII GGWCC 3 cut(s) 34, 303, 329
BaeGI GKGCMC 1 cut(s) 615
BamHI GGATCC 1 cut(s) 313
BanI GGYRCC 1 cut(s) 164
BbvI GCAGC 8 cut(s) 142, 146, 246, 264, 265, 268, 286, 551
BceAI ACGGC 5 cut(s) 109, 123, 152, 204, 398
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BciT130I CCWGG 2 cut(s) 55, 326
BcnI CCSGG 6 cut(s) 112, 301, 318, 342, 343, 432
BcoDI GTCTC 1 cut(s) 467
BfoI RGCGCY 1 cut(s) 168
Bme1390I CCNGG 8 cut(s) 55, 112, 301, 318, 326, 342, 343, 432
Bme18I GGWCC 3 cut(s) 34, 303, 329
BmeT110I CYCGRG 3 cut(s) 65, 341, 689
BmgT120I GGNCC 3 cut(s) 34, 303, 329
BmiI GGNNCC 5 cut(s) 166, 304, 315, 330, 661
BmrFI CCNGG 8 cut(s) 55, 112, 301, 318, 326, 342, 343, 432
BmsI GCATC 2 cut(s) 88, 484
BpmI CTGGAG 1 cut(s) 488
BpuMI CCSGG 6 cut(s) 112, 301, 318, 342, 343, 432
BsaBI GATNNNNATC 1 cut(s) 312
BsaHI GRCGYC 3 cut(s) 165, 428, 437
BsaJI CCNNGG 6 cut(s) 248, 325, 340, 341, 363, 431
BsaWI WCCGGW 3 cut(s) 36, 146, 331
Bsc4I CCNNNNNNNGG 8 cut(s) 117, 153, 331, 346, 347, 348, 364, 414
Bse8I GATNNNNATC 1 cut(s) 312
BseAI TCCGGA 1 cut(s) 36
BseBI CCWGG 2 cut(s) 55, 326
BseDI CCNNGG 6 cut(s) 248, 325, 340, 341, 363, 431
BseGI GGATG 2 cut(s) 229, 357
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 8 cut(s) 117, 153, 331, 346, 347, 348, 364, 414
BseMII CTCAG 3 cut(s) 25, 65, 128
BseRI GAGGAG 4 cut(s) 130, 133, 485, 518
BseSI GKGCMC 1 cut(s) 615
BseX3I CGGCCG 2 cut(s) 108, 297
BseXI GCAGC 8 cut(s) 142, 146, 246, 264, 265, 268, 286, 551
Bsh1236I CGCG 4 cut(s) 250, 365, 410, 593
Bsh1285I CGRYCG 2 cut(s) 111, 300
BshFI GGCC 5 cut(s) 53, 110, 191, 299, 407
BshNI GGYRCC 1 cut(s) 164
BsiEI CGRYCG 2 cut(s) 111, 300
BsiHKCI CYCGRG 3 cut(s) 65, 341, 689
BsiSI CCGG 8 cut(s) 37, 111, 147, 300, 317, 332, 342, 431
BsiWI CGTACG 1 cut(s) 680
BslFI GGGAC 2 cut(s) 448, 651
BslI CCNNNNNNNGG 8 cut(s) 117, 153, 331, 346, 347, 348, 364, 414
BsmAI GTCTC 1 cut(s) 467
BsmBI CGTCTC 1 cut(s) 467
BsmFI GGGAC 2 cut(s) 448, 651
BsnI GGCC 5 cut(s) 53, 110, 191, 299, 407
BsoBI CYCGRG 3 cut(s) 65, 341, 689
Bsp1286I GDGCHC 1 cut(s) 615
Bsp13I TCCGGA 1 cut(s) 36
Bsp143I GATC 3 cut(s) 60, 313, 684
BspANI GGCC 5 cut(s) 53, 110, 191, 299, 407
BspCNI CTCAG 3 cut(s) 24, 66, 129
BspEI TCCGGA 1 cut(s) 36
BspFNI CGCG 4 cut(s) 250, 365, 410, 593
BspLI GGNNCC 5 cut(s) 166, 304, 315, 330, 661
BspPI GGATC 4 cut(s) 55, 308, 321, 679
BspT107I GGYRCC 1 cut(s) 164
BssECI CCNNGG 6 cut(s) 248, 325, 340, 341, 363, 431
BssMI GATC 3 cut(s) 60, 313, 684
BssNI GRCGYC 3 cut(s) 165, 428, 437
Bst2UI CCWGG 2 cut(s) 55, 326
Bst4CI ACNGT 3 cut(s) 34, 202, 680
BstACI GRCGYC 3 cut(s) 165, 428, 437
BstAPI GCANNNNNTGC 1 cut(s) 259
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 3 cut(s) 11, 74, 137
BstDSI CCRYGG 2 cut(s) 248, 363
BstF5I GGATG 2 cut(s) 229, 357
BstFNI CGCG 4 cut(s) 250, 365, 410, 593
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 2 cut(s) 167, 593
BstKTI GATC 3 cut(s) 63, 316, 687
BstMAI GTCTC 1 cut(s) 467
BstMBI GATC 3 cut(s) 60, 313, 684
BstMCI CGRYCG 2 cut(s) 111, 300
BstNI CCWGG 2 cut(s) 55, 326
BstSCI CCNGG 8 cut(s) 53, 110, 299, 316, 324, 340, 341, 430
BstSLI GKGCMC 1 cut(s) 615
BstUI CGCG 4 cut(s) 250, 365, 410, 593
BstV1I GCAGC 8 cut(s) 142, 146, 246, 264, 265, 268, 286, 551
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BstZI CGGCCG 2 cut(s) 108, 297
BsuRI GGCC 5 cut(s) 53, 110, 191, 299, 407
BtgI CCRYGG 2 cut(s) 248, 363
BtgZI GCGATG 2 cut(s) 111, 507
BtsCI GGATG 2 cut(s) 229, 357
Cac8I GCNNGC 1 cut(s) 294
CaiI CAGNNNCTG 3 cut(s) 136, 259, 280
CfoI GCGC 2 cut(s) 167, 593
Cfr13I GGNCC 3 cut(s) 34, 303, 329
Cfr42I CCGCGG 2 cut(s) 251, 366
Cfr9I CCCGGG 1 cut(s) 341
CpoI CGGWCCG 1 cut(s) 34
CseI GACGC 3 cut(s) 399, 436, 445
Csp6I GTAC 1 cut(s) 681
CspI CGGWCCG 1 cut(s) 34
CviAII CATG 2 cut(s) 574, 606
CviQI GTAC 1 cut(s) 681
DdeI CTNAG 3 cut(s) 11, 74, 137
DinI GGCGCC 1 cut(s) 166
DpnI GATC 3 cut(s) 62, 315, 686
DpnII GATC 3 cut(s) 60, 313, 684
EaeI YGGCCR 3 cut(s) 108, 189, 297
EagI CGGCCG 2 cut(s) 108, 297
EciI GGCGGA 4 cut(s) 222, 392, 413, 515
EclXI CGGCCG 2 cut(s) 108, 297
Eco32I GATATC 1 cut(s) 703
Eco47I GGWCC 3 cut(s) 34, 303, 329
Eco52I CGGCCG 2 cut(s) 108, 297
Eco88I CYCGRG 3 cut(s) 65, 341, 689
EcoRII CCWGG 2 cut(s) 53, 324
EcoRV GATATC 1 cut(s) 703
EgeI GGCGCC 1 cut(s) 166
EheI GGCGCC 1 cut(s) 166
Esp3I CGTCTC 1 cut(s) 467
FaeI CATG 2 cut(s) 577, 609
FaiI YATR 3 cut(s) 575, 602, 607
FaqI GGGAC 2 cut(s) 448, 651
FatI CATG 2 cut(s) 573, 605
FokI GGATG 2 cut(s) 216, 364
GlaI GCGC 2 cut(s) 166, 592
GsuI CTGGAG 1 cut(s) 488
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 5 cut(s) 53, 110, 191, 299, 407
HapII CCGG 8 cut(s) 37, 111, 147, 300, 317, 332, 342, 431
HgaI GACGC 3 cut(s) 399, 436, 445
HhaI GCGC 2 cut(s) 167, 593
Hin1I GRCGYC 3 cut(s) 165, 428, 437
Hin1II CATG 2 cut(s) 577, 609
Hin6I GCGC 2 cut(s) 165, 591
HinP1I GCGC 2 cut(s) 165, 591
HincII GTYRAC 2 cut(s) 8, 337
HindII GTYRAC 2 cut(s) 8, 337
HinfI GANTC 7 cut(s) 4, 150, 283, 308, 554, 652, 694
HpaII CCGG 8 cut(s) 37, 111, 147, 300, 317, 332, 342, 431
Hpy166II GTNNAC 2 cut(s) 8, 337
Hpy188I TCNGA 4 cut(s) 75, 307, 313, 710
Hpy188III TCNNGA 4 cut(s) 37, 392, 691, 698
Hpy8I GTNNAC 2 cut(s) 8, 337
Hpy99I CGWCG 7 cut(s) 95, 109, 206, 215, 415, 430, 538
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 3 cut(s) 34, 202, 680
HpyCH4V TGCA 4 cut(s) 25, 573, 624, 675
HpyF3I CTNAG 3 cut(s) 11, 74, 137
Hsp92I GRCGYC 3 cut(s) 165, 428, 437
Hsp92II CATG 2 cut(s) 577, 609
HspAI GCGC 2 cut(s) 165, 591
KasI GGCGCC 1 cut(s) 164
Kpn2I TCCGGA 1 cut(s) 36
KspI CCGCGG 2 cut(s) 251, 366
Kzo9I GATC 3 cut(s) 60, 313, 684
LmnI GCTCC 2 cut(s) 120, 665
Lsp1109I GCAGC 8 cut(s) 142, 146, 246, 264, 265, 268, 286, 551
LweI GCATC 2 cut(s) 88, 484
MalI GATC 3 cut(s) 62, 315, 686
MboI GATC 3 cut(s) 60, 313, 684
MboII GAAGA 3 cut(s) 227, 595, 697
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 615
Mly113I GGCGCC 1 cut(s) 165
MlyI GAGTC 1 cut(s) 13
MroI TCCGGA 1 cut(s) 36
MspA1I CMGCKG 5 cut(s) 133, 250, 259, 277, 365
MspI CCGG 8 cut(s) 37, 111, 147, 300, 317, 332, 342, 431
MspR9I CCNGG 8 cut(s) 55, 112, 301, 318, 326, 342, 343, 432
MvaI CCWGG 2 cut(s) 55, 326
MvnI CGCG 4 cut(s) 250, 365, 410, 593
NarI GGCGCC 1 cut(s) 165
NciI CCSGG 6 cut(s) 112, 301, 318, 342, 343, 432
NdeII GATC 3 cut(s) 60, 313, 684
NlaIII CATG 2 cut(s) 577, 609
NlaIV GGNNCC 5 cut(s) 166, 304, 315, 330, 661
PaeR7I CTCGAG 2 cut(s) 65, 689
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 696
PfeI GAWTC 6 cut(s) 150, 283, 308, 554, 652, 694
Pfl23II CGTACG 1 cut(s) 680
PleI GAGTC 1 cut(s) 12
PluTI GGCGCC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 12
Psp6I CCWGG 2 cut(s) 53, 324
PspGI CCWGG 2 cut(s) 53, 324
PspLI CGTACG 1 cut(s) 680
PspN4I GGNNCC 5 cut(s) 166, 304, 315, 330, 661
PspPI GGNCC 3 cut(s) 34, 303, 329
PspXI VCTCGAGB 1 cut(s) 65
PsrI GAACNNNNNNTAC 2 cut(s) 210, 242
PstNI CAGNNNCTG 3 cut(s) 136, 259, 280
PsuI RGATCY 1 cut(s) 313
PvuII CAGCTG 2 cut(s) 259, 277
RsaI GTAC 1 cut(s) 682
RsaNI GTAC 1 cut(s) 681
Rsr2I CGGWCCG 1 cut(s) 34
RsrII CGGWCCG 1 cut(s) 34
SacII CCGCGG 2 cut(s) 251, 366
Sau3AI GATC 3 cut(s) 60, 313, 684
Sau96I GGNCC 3 cut(s) 34, 303, 329
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 8 cut(s) 55, 112, 301, 318, 326, 342, 343, 432
SduI GDGCHC 1 cut(s) 615
SetI ASST 8 cut(s) 72, 125, 228, 261, 270, 279, 360, 645
SfaNI GCATC 2 cut(s) 88, 484
SfoI GGCGCC 1 cut(s) 166
Sfr274I CTCGAG 2 cut(s) 65, 689
Sfr303I CCGCGG 2 cut(s) 251, 366
SgrBI CCGCGG 2 cut(s) 251, 366
SinI GGWCC 3 cut(s) 34, 303, 329
SlaI CTCGAG 2 cut(s) 65, 689
SmaI CCCGGG 1 cut(s) 343
SmlI CTYRAG 2 cut(s) 65, 689
SmoI CTYRAG 2 cut(s) 65, 689
SspDI GGCGCC 1 cut(s) 164
StyD4I CCNGG 8 cut(s) 53, 110, 299, 316, 324, 340, 341, 430
TaaI ACNGT 3 cut(s) 34, 202, 680
TaqI TCGA 6 cut(s) 66, 204, 635, 655, 690, 699
TfiI GAWTC 6 cut(s) 150, 283, 308, 554, 652, 694
TseI GCWGC 8 cut(s) 130, 159, 253, 256, 259, 274, 277, 564
TspDTI ATGAA 2 cut(s) 368, 589
TspGWI ACGGA 2 cut(s) 199, 491
TspMI CCCGGG 1 cut(s) 341
VpaK11BI GGWCC 3 cut(s) 34, 303, 329
XhoI CTCGAG 2 cut(s) 65, 689
XmaI CCCGGG 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.