Rh5AG014700

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007033
Physical Location & Seq
Reverse (-)
977122 .. 983156
6035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG014700.1

Sequence Viewer

Length: 813 bp
ATGGAACTCAGAGTCCCCAAATCATGGGTTGGATGGACAGATGACATGGCCGGAGGATGGGGAGTTGATTTGGGTAAATACTCACGGGTCTTCCATGGCCTCGCTTTAAATCCAAACTCGCAGGAAACGGTCCGGAGAGGAAGGGAGGCCAGGACGATCCTCGAGGTTCTGAGAGAGCAAATGGACGACGGCGATGCGGCGACGGCCGGGAGGAGGAGCTTCAAGCAGCGGCTGAGATTGACCGGAATCGGGTGCTGCGGCGCCGTCTGGGGATTTGGACGGACGGCCACGATTACCGTCGAGGACGATGAAGAACAACGCCTACATCCGCCGCAACAACAACCGCGGCAGCAGCTGCCGAACCTATCGCAGCTGCTGATTCTGGGGCAGGCGGCCGGGTCCGATTCGGATCCGGGTTGCCTGGGTCCGGTCAACCCCGGGTCGGGGATGAACCTCGCCGCGGCTCTGGCGGCGGAACGGCATTATCGGGAGGCGGACGAGGCCGCGTCGGGCGTTGGGGCGGCTCCGGGGACGCCGATGAGGGTGTCGCTGATGAGATTGCTGGAGGAGACGGAAATGGACGGGTGCGATGCGGCGGAGGAGAAAAATGAGAAAGCGGCGGCGGCGGCGGCGGCGACGGGGGTGGTGGGTTGTGATTCGGTGTGCTGCGTGTGCATGGGGAGGAAGAAAGGCGCGGCGTTCATACCATGTGGGCACACTTTTTGCAGGGTGTGTTCGAGGGAGGTGTGGCTGAATCGAGGCTCCTGTCCCCTCTGCAACCGTACGATCCTCGAGATTCTCGATATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

270

Amino Acids

29.02

Weight (kDa)

5.68

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 220 - 264 7.1e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 222 - 259 1.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 222 - 259 2e-06 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 222 - 263 8.9e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 161
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 24
AccII CGCG 4 cut(s) 346, 461, 506, 695
AccIII TCCGGA 1 cut(s) 132
AclWI GGATC 4 cut(s) 151, 404, 417, 781
AcoI YGGCCR 4 cut(s) 48, 204, 285, 393
AcyI GRCGYC 2 cut(s) 261, 533
AfaI GTAC 1 cut(s) 784
AfiI CCNNNNNNNGG 9 cut(s) 24, 57, 213, 249, 427, 442, 443, 444, 460
AgsI TTSAA 1 cut(s) 223
AjnI CCWGG 2 cut(s) 149, 420
AloI GAACNNNNNNTCC 1 cut(s) 29
AluBI AGCT 3 cut(s) 219, 355, 373
AluI AGCT 3 cut(s) 219, 355, 373
Alw26I GTCTC 1 cut(s) 563
AlwI GGATC 4 cut(s) 151, 404, 417, 781
AlwNI CAGNNNCTG 3 cut(s) 232, 355, 376
Ama87I CYCGRG 3 cut(s) 161, 437, 791
Aor13HI TCCGGA 1 cut(s) 132
AoxI GGCC 7 cut(s) 48, 97, 147, 204, 285, 393, 501
ApeKI GCWGC 8 cut(s) 226, 255, 349, 352, 355, 370, 373, 666
AspLEI GCGC 2 cut(s) 263, 695
AspS9I GGNCC 3 cut(s) 130, 399, 425
AsuC2I CCSGG 6 cut(s) 208, 397, 414, 438, 439, 528
AvaI CYCGRG 3 cut(s) 161, 437, 791
AvaII GGWCC 3 cut(s) 130, 399, 425
BaeGI GKGCMC 1 cut(s) 717
BamHI GGATCC 1 cut(s) 409
BanI GGYRCC 1 cut(s) 260
BbsI GAAGAC 1 cut(s) 82
BbvI GCAGC 8 cut(s) 238, 242, 342, 360, 361, 364, 382, 653
BccI CCATC 2 cut(s) 27, 51
BceAI ACGGC 5 cut(s) 205, 219, 248, 300, 494
BcgI CGANNNNNNTGC 2 cut(s) 176, 210
BciT130I CCWGG 2 cut(s) 151, 422
BcnI CCSGG 6 cut(s) 208, 397, 414, 438, 439, 528
BcoDI GTCTC 1 cut(s) 563
BfoI RGCGCY 1 cut(s) 264
Bme1390I CCNGG 8 cut(s) 151, 208, 397, 414, 422, 438, 439, 528
Bme18I GGWCC 3 cut(s) 130, 399, 425
BmeT110I CYCGRG 3 cut(s) 161, 437, 791
BmgT120I GGNCC 3 cut(s) 130, 399, 425
BmiI GGNNCC 6 cut(s) 262, 400, 411, 426, 525, 763
BmrFI CCNGG 8 cut(s) 151, 208, 397, 414, 422, 438, 439, 528
BmsI GCATC 2 cut(s) 184, 580
BpiI GAAGAC 1 cut(s) 82
BpmI CTGGAG 1 cut(s) 584
BpuMI CCSGG 6 cut(s) 208, 397, 414, 438, 439, 528
BsaBI GATNNNNATC 1 cut(s) 408
BsaHI GRCGYC 2 cut(s) 261, 533
BsaJI CCNNGG 7 cut(s) 94, 344, 421, 436, 437, 459, 527
BsaWI WCCGGW 3 cut(s) 132, 242, 427
Bsc4I CCNNNNNNNGG 9 cut(s) 24, 57, 213, 249, 427, 442, 443, 444, 460
Bse8I GATNNNNATC 1 cut(s) 408
BseAI TCCGGA 1 cut(s) 132
BseBI CCWGG 2 cut(s) 151, 422
BseDI CCNNGG 7 cut(s) 94, 344, 421, 436, 437, 459, 527
BseGI GGATG 4 cut(s) 38, 62, 325, 453
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 9 cut(s) 24, 57, 213, 249, 427, 442, 443, 444, 460
BseMII CTCAG 3 cut(s) 22, 161, 224
BseRI GAGGAG 4 cut(s) 226, 229, 581, 614
BseSI GKGCMC 1 cut(s) 717
BseX3I CGGCCG 2 cut(s) 204, 393
BseXI GCAGC 8 cut(s) 238, 242, 342, 360, 361, 364, 382, 653
Bsh1236I CGCG 4 cut(s) 346, 461, 506, 695
Bsh1285I CGRYCG 2 cut(s) 207, 396
BshFI GGCC 7 cut(s) 50, 99, 149, 206, 287, 395, 503
BshNI GGYRCC 1 cut(s) 260
BsiEI CGRYCG 2 cut(s) 207, 396
BsiHKCI CYCGRG 3 cut(s) 161, 437, 791
BsiSI CCGG 9 cut(s) 51, 133, 207, 243, 396, 413, 428, 438, 527
BsiWI CGTACG 1 cut(s) 782
BslFI GGGAC 2 cut(s) 544, 753
BslI CCNNNNNNNGG 9 cut(s) 24, 57, 213, 249, 427, 442, 443, 444, 460
BsmAI GTCTC 1 cut(s) 563
BsmBI CGTCTC 1 cut(s) 563
BsmFI GGGAC 2 cut(s) 544, 753
BsnI GGCC 7 cut(s) 50, 99, 149, 206, 287, 395, 503
BsoBI CYCGRG 3 cut(s) 161, 437, 791
Bsp1286I GDGCHC 1 cut(s) 717
Bsp13I TCCGGA 1 cut(s) 132
Bsp143I GATC 3 cut(s) 156, 409, 786
Bsp19I CCATGG 1 cut(s) 94
BspANI GGCC 7 cut(s) 50, 99, 149, 206, 287, 395, 503
BspCNI CTCAG 3 cut(s) 21, 162, 225
BspEI TCCGGA 1 cut(s) 132
BspFNI CGCG 4 cut(s) 346, 461, 506, 695
BspLI GGNNCC 6 cut(s) 262, 400, 411, 426, 525, 763
BspPI GGATC 4 cut(s) 151, 404, 417, 781
BspT107I GGYRCC 1 cut(s) 260
BssECI CCNNGG 7 cut(s) 94, 344, 421, 436, 437, 459, 527
BssMI GATC 3 cut(s) 156, 409, 786
BssNI GRCGYC 2 cut(s) 261, 533
BssT1I CCWWGG 1 cut(s) 94
Bst2UI CCWGG 2 cut(s) 151, 422
Bst4CI ACNGT 3 cut(s) 130, 298, 782
BstACI GRCGYC 2 cut(s) 261, 533
BstAPI GCANNNNNTGC 1 cut(s) 355
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 3 cut(s) 8, 170, 233
BstDSI CCRYGG 3 cut(s) 94, 344, 459
BstF5I GGATG 4 cut(s) 38, 62, 325, 453
BstFNI CGCG 4 cut(s) 346, 461, 506, 695
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 2 cut(s) 263, 695
BstKTI GATC 3 cut(s) 159, 412, 789
BstMAI GTCTC 1 cut(s) 563
BstMBI GATC 3 cut(s) 156, 409, 786
BstMCI CGRYCG 2 cut(s) 207, 396
BstNI CCWGG 2 cut(s) 151, 422
BstSCI CCNGG 8 cut(s) 149, 206, 395, 412, 420, 436, 437, 526
BstSLI GKGCMC 1 cut(s) 717
BstUI CGCG 4 cut(s) 346, 461, 506, 695
BstV1I GCAGC 8 cut(s) 238, 242, 342, 360, 361, 364, 382, 653
BstV2I GAAGAC 1 cut(s) 82
BstX2I RGATCY 1 cut(s) 409
BstYI RGATCY 1 cut(s) 409
BstZI CGGCCG 2 cut(s) 204, 393
BsuRI GGCC 7 cut(s) 50, 99, 149, 206, 287, 395, 503
BtgI CCRYGG 3 cut(s) 94, 344, 459
BtgZI GCGATG 2 cut(s) 207, 603
BtsCI GGATG 4 cut(s) 38, 62, 325, 453
Cac8I GCNNGC 1 cut(s) 390
CaiI CAGNNNCTG 3 cut(s) 232, 355, 376
CfoI GCGC 2 cut(s) 263, 695
Cfr13I GGNCC 3 cut(s) 130, 399, 425
Cfr42I CCGCGG 2 cut(s) 347, 462
Cfr9I CCCGGG 1 cut(s) 437
CpoI CGGWCCG 1 cut(s) 130
CseI GACGC 2 cut(s) 495, 541
Csp6I GTAC 1 cut(s) 783
CspI CGGWCCG 1 cut(s) 130
CviAII CATG 5 cut(s) 24, 46, 95, 676, 708
CviQI GTAC 1 cut(s) 783
DdeI CTNAG 3 cut(s) 8, 170, 233
DinI GGCGCC 1 cut(s) 262
DpnI GATC 3 cut(s) 158, 411, 788
DpnII GATC 3 cut(s) 156, 409, 786
DraI TTTAAA 1 cut(s) 108
EaeI YGGCCR 4 cut(s) 48, 204, 285, 393
EagI CGGCCG 2 cut(s) 204, 393
EciI GGCGGA 4 cut(s) 318, 488, 509, 611
EclXI CGGCCG 2 cut(s) 204, 393
Eco130I CCWWGG 1 cut(s) 94
Eco32I GATATC 1 cut(s) 805
Eco47I GGWCC 3 cut(s) 130, 399, 425
Eco52I CGGCCG 2 cut(s) 204, 393
Eco88I CYCGRG 3 cut(s) 161, 437, 791
EcoRII CCWGG 2 cut(s) 149, 420
EcoRV GATATC 1 cut(s) 805
EcoT14I CCWWGG 1 cut(s) 94
EgeI GGCGCC 1 cut(s) 262
EheI GGCGCC 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 94
Esp3I CGTCTC 1 cut(s) 563
FaeI CATG 5 cut(s) 27, 49, 98, 679, 711
FaiI YATR 6 cut(s) 25, 47, 96, 677, 704, 709
FaqI GGGAC 2 cut(s) 544, 753
FatI CATG 5 cut(s) 23, 45, 94, 675, 707
FokI GGATG 4 cut(s) 45, 69, 312, 460
GlaI GCGC 2 cut(s) 262, 694
GsuI CTGGAG 1 cut(s) 584
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 7 cut(s) 50, 99, 149, 206, 287, 395, 503
HapII CCGG 9 cut(s) 51, 133, 207, 243, 396, 413, 428, 438, 527
HgaI GACGC 2 cut(s) 495, 541
HhaI GCGC 2 cut(s) 263, 695
Hin1I GRCGYC 2 cut(s) 261, 533
Hin1II CATG 5 cut(s) 27, 49, 98, 679, 711
Hin6I GCGC 2 cut(s) 261, 693
HinP1I GCGC 2 cut(s) 261, 693
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HinfI GANTC 7 cut(s) 12, 246, 379, 404, 656, 754, 796
HpaII CCGG 9 cut(s) 51, 133, 207, 243, 396, 413, 428, 438, 527
Hpy166II GTNNAC 1 cut(s) 433
Hpy188I TCNGA 5 cut(s) 11, 171, 403, 409, 812
Hpy188III TCNNGA 4 cut(s) 133, 488, 793, 800
Hpy8I GTNNAC 1 cut(s) 433
Hpy99I CGWCG 5 cut(s) 191, 205, 302, 511, 640
HpyAV CCTTC 1 cut(s) 135
HpyCH4III ACNGT 3 cut(s) 130, 298, 782
HpyCH4V TGCA 3 cut(s) 675, 726, 777
HpyF3I CTNAG 3 cut(s) 8, 170, 233
Hsp92I GRCGYC 2 cut(s) 261, 533
Hsp92II CATG 5 cut(s) 27, 49, 98, 679, 711
HspAI GCGC 2 cut(s) 261, 693
KasI GGCGCC 1 cut(s) 260
Kpn2I TCCGGA 1 cut(s) 132
KspI CCGCGG 2 cut(s) 347, 462
Kzo9I GATC 3 cut(s) 156, 409, 786
LmnI GCTCC 3 cut(s) 216, 529, 767
Lsp1109I GCAGC 8 cut(s) 238, 242, 342, 360, 361, 364, 382, 653
LweI GCATC 2 cut(s) 184, 580
MalI GATC 3 cut(s) 158, 411, 788
MboI GATC 3 cut(s) 156, 409, 786
MboII GAAGA 4 cut(s) 82, 323, 697, 799
MflI RGATCY 1 cut(s) 409
MhlI GDGCHC 1 cut(s) 717
Mly113I GGCGCC 1 cut(s) 261
MlyI GAGTC 1 cut(s) 21
MmeI TCCRAC 1 cut(s) 10
MroI TCCGGA 1 cut(s) 132
MseI TTAA 1 cut(s) 107
MspA1I CMGCKG 5 cut(s) 229, 346, 355, 373, 461
MspI CCGG 9 cut(s) 51, 133, 207, 243, 396, 413, 428, 438, 527
MspR9I CCNGG 8 cut(s) 151, 208, 397, 414, 422, 438, 439, 528
MvaI CCWGG 2 cut(s) 151, 422
MvnI CGCG 4 cut(s) 346, 461, 506, 695
NarI GGCGCC 1 cut(s) 261
NciI CCSGG 6 cut(s) 208, 397, 414, 438, 439, 528
NcoI CCATGG 1 cut(s) 94
NdeII GATC 3 cut(s) 156, 409, 786
NlaIII CATG 5 cut(s) 27, 49, 98, 679, 711
NlaIV GGNNCC 6 cut(s) 262, 400, 411, 426, 525, 763
PaeR7I CTCGAG 2 cut(s) 161, 791
PcsI WCGNNNNNNNCGW 1 cut(s) 798
PfeI GAWTC 6 cut(s) 246, 379, 404, 656, 754, 796
Pfl23II CGTACG 1 cut(s) 782
PflMI CCANNNNNTGG 1 cut(s) 24
PleI GAGTC 1 cut(s) 20
PluTI GGCGCC 1 cut(s) 264
PpsI GAGTC 1 cut(s) 20
Psp6I CCWGG 2 cut(s) 149, 420
PspGI CCWGG 2 cut(s) 149, 420
PspLI CGTACG 1 cut(s) 782
PspN4I GGNNCC 6 cut(s) 262, 400, 411, 426, 525, 763
PspPI GGNCC 3 cut(s) 130, 399, 425
PspXI VCTCGAGB 1 cut(s) 161
PsrI GAACNNNNNNTAC 2 cut(s) 306, 338
PstNI CAGNNNCTG 3 cut(s) 232, 355, 376
PsuI RGATCY 1 cut(s) 409
PvuII CAGCTG 2 cut(s) 355, 373
RsaI GTAC 1 cut(s) 784
RsaNI GTAC 1 cut(s) 783
Rsr2I CGGWCCG 1 cut(s) 130
RsrII CGGWCCG 1 cut(s) 130
SacII CCGCGG 2 cut(s) 347, 462
SaqAI TTAA 1 cut(s) 107
Sau3AI GATC 3 cut(s) 156, 409, 786
Sau96I GGNCC 3 cut(s) 130, 399, 425
SchI GAGTC 1 cut(s) 21
ScrFI CCNGG 8 cut(s) 151, 208, 397, 414, 422, 438, 439, 528
SduI GDGCHC 1 cut(s) 717
SetI ASST 7 cut(s) 168, 221, 357, 366, 375, 456, 747
SfaNI GCATC 2 cut(s) 184, 580
SfoI GGCGCC 1 cut(s) 262
Sfr274I CTCGAG 2 cut(s) 161, 791
Sfr303I CCGCGG 2 cut(s) 347, 462
SgrBI CCGCGG 2 cut(s) 347, 462
SinI GGWCC 3 cut(s) 130, 399, 425
SlaI CTCGAG 2 cut(s) 161, 791
SmaI CCCGGG 1 cut(s) 439
SmlI CTYRAG 2 cut(s) 161, 791
SmoI CTYRAG 2 cut(s) 161, 791
SspDI GGCGCC 1 cut(s) 260
StyD4I CCNGG 8 cut(s) 149, 206, 395, 412, 420, 436, 437, 526
StyI CCWWGG 1 cut(s) 94
TaaI ACNGT 3 cut(s) 130, 298, 782
TaqI TCGA 6 cut(s) 162, 300, 737, 757, 792, 801
TfiI GAWTC 6 cut(s) 246, 379, 404, 656, 754, 796
Tru1I TTAA 1 cut(s) 107
Tru9I TTAA 1 cut(s) 107
TseI GCWGC 8 cut(s) 226, 255, 349, 352, 355, 370, 373, 666
TspDTI ATGAA 3 cut(s) 324, 464, 691
TspGWI ACGGA 2 cut(s) 295, 587
TspMI CCCGGG 1 cut(s) 437
Van91I CCANNNNNTGG 1 cut(s) 24
VpaK11BI GGWCC 3 cut(s) 130, 399, 425
XhoI CTCGAG 2 cut(s) 161, 791
XmaI CCCGGG 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.