Rh5DG016200

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007033
Physical Location & Seq
Reverse (-)
1225766 .. 1227504
1739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG016200.1

Sequence Viewer

Length: 621 bp
ATGGACGACGGCGATGCGGCCACTGGAGGGAGGAGGAGCTTCAAGCAGCGGCTGAGATTGACCGGAATCGGGTGCTGCGGCGCCGTCTGGGGATTTGGACGGACGGCCACGATTACCGTCGAGGACGATGAAGAACAACACGTACATCCACCGCAACAACAACCGCGGCAGCAGCTGCCGAACCTATCGCAGCTGCTGATTCTGGGGCAGGCGGCCGGGTCCGATTCGGATCCGGGTTGCCTGGGTCCGGTCAACCCCGGGTCGGGGATGAACCTCGCCGCGGCTCTGGCGGCGGAACGGCATTATCGGGAGGCGGACGAGGCCGCGTCGGGTGGTGGGGCGGCGCCGGGGACGCCGATGAGGGTGTCGCTGATGAGATTGCTGGAGGAGACGGAAATGGACGGGTGCGATGCGGCGGAGGAGAAAAATGAGAAAGCGGCGGCGGTGGGGGTGGTGGGTTGTGATTCGGTGTGCTGCGTGTGCATGGGGAGGAAGAAAGGCGCGGCGTTCATACCATGTGGGCACACTTTTTGCAGGGTGTGTTCGAGGGAGGTGTGGCTGAATCGAGGCTCCTGTCCCCTCTGCAACCGTACGATCCTCGAGATTCTCGATATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

206

Amino Acids

21.79

Weight (kDa)

5.25

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 156 - 200 4.5e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 158 - 195 1.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 158 - 195 1.3e-06 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 158 - 199 5.8e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 80, 343
AccII CGCG 4 cut(s) 166, 281, 326, 503
AclWI GGATC 3 cut(s) 224, 237, 589
AcoI YGGCCR 3 cut(s) 18, 105, 213
AcyI GRCGYC 3 cut(s) 81, 344, 353
AfaI GTAC 2 cut(s) 144, 592
AfiI CCNNNNNNNGG 7 cut(s) 27, 69, 247, 262, 263, 264, 280
AflIII ACRYGT 1 cut(s) 139
AgsI TTSAA 1 cut(s) 43
AjnI CCWGG 1 cut(s) 240
AluBI AGCT 3 cut(s) 39, 175, 193
AluI AGCT 3 cut(s) 39, 175, 193
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 3 cut(s) 224, 237, 589
AlwNI CAGNNNCTG 3 cut(s) 52, 175, 196
Ama87I CYCGRG 2 cut(s) 257, 599
AoxI GGCC 4 cut(s) 18, 105, 213, 321
ApeKI GCWGC 8 cut(s) 46, 75, 169, 172, 175, 190, 193, 474
AspLEI GCGC 3 cut(s) 83, 346, 503
AspS9I GGNCC 2 cut(s) 219, 245
AsuC2I CCSGG 5 cut(s) 217, 234, 258, 259, 348
AvaI CYCGRG 2 cut(s) 257, 599
AvaII GGWCC 2 cut(s) 219, 245
BaeGI GKGCMC 1 cut(s) 525
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 2 cut(s) 80, 343
BbvI GCAGC 8 cut(s) 58, 62, 162, 180, 181, 184, 202, 461
BceAI ACGGC 4 cut(s) 25, 68, 120, 314
BcgI CGANNNNNNTGC 1 cut(s) 30
BciT130I CCWGG 1 cut(s) 242
BcnI CCSGG 5 cut(s) 217, 234, 258, 259, 348
BcoDI GTCTC 1 cut(s) 383
BfoI RGCGCY 2 cut(s) 84, 347
Bme1390I CCNGG 6 cut(s) 217, 234, 242, 258, 259, 348
Bme18I GGWCC 2 cut(s) 219, 245
BmeT110I CYCGRG 2 cut(s) 257, 599
BmgT120I GGNCC 2 cut(s) 219, 245
BmiI GGNNCC 6 cut(s) 82, 220, 231, 246, 345, 571
BmrFI CCNGG 6 cut(s) 217, 234, 242, 258, 259, 348
BmsI GCATC 2 cut(s) 4, 400
BpmI CTGGAG 2 cut(s) 45, 404
BpuMI CCSGG 5 cut(s) 217, 234, 258, 259, 348
BsaAI YACGTR 1 cut(s) 142
BsaBI GATNNNNATC 1 cut(s) 228
BsaHI GRCGYC 3 cut(s) 81, 344, 353
BsaJI CCNNGG 6 cut(s) 164, 241, 256, 257, 279, 347
BsaWI WCCGGW 2 cut(s) 62, 247
Bsc4I CCNNNNNNNGG 7 cut(s) 27, 69, 247, 262, 263, 264, 280
Bse1I ACTGG 1 cut(s) 28
Bse8I GATNNNNATC 1 cut(s) 228
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 6 cut(s) 164, 241, 256, 257, 279, 347
BseGI GGATG 2 cut(s) 145, 273
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 7 cut(s) 27, 69, 247, 262, 263, 264, 280
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 4 cut(s) 46, 49, 401, 434
BseSI GKGCMC 1 cut(s) 525
BseX3I CGGCCG 1 cut(s) 213
BseXI GCAGC 8 cut(s) 58, 62, 162, 180, 181, 184, 202, 461
Bsh1236I CGCG 4 cut(s) 166, 281, 326, 503
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 4 cut(s) 20, 107, 215, 323
BshNI GGYRCC 2 cut(s) 80, 343
BsiEI CGRYCG 1 cut(s) 216
BsiHKCI CYCGRG 2 cut(s) 257, 599
BsiSI CCGG 6 cut(s) 63, 216, 233, 248, 258, 347
BsiWI CGTACG 1 cut(s) 590
BslFI GGGAC 2 cut(s) 364, 561
BslI CCNNNNNNNGG 7 cut(s) 27, 69, 247, 262, 263, 264, 280
BsmAI GTCTC 1 cut(s) 383
BsmBI CGTCTC 1 cut(s) 383
BsmFI GGGAC 2 cut(s) 364, 561
BsnI GGCC 4 cut(s) 20, 107, 215, 323
BsoBI CYCGRG 2 cut(s) 257, 599
Bsp1286I GDGCHC 1 cut(s) 525
Bsp143I GATC 2 cut(s) 229, 594
BspANI GGCC 4 cut(s) 20, 107, 215, 323
BspCNI CTCAG 1 cut(s) 45
BspFNI CGCG 4 cut(s) 166, 281, 326, 503
BspLI GGNNCC 6 cut(s) 82, 220, 231, 246, 345, 571
BspPI GGATC 3 cut(s) 224, 237, 589
BspT107I GGYRCC 2 cut(s) 80, 343
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 6 cut(s) 164, 241, 256, 257, 279, 347
BssMI GATC 2 cut(s) 229, 594
BssNI GRCGYC 3 cut(s) 81, 344, 353
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 2 cut(s) 118, 590
BstACI GRCGYC 3 cut(s) 81, 344, 353
BstAPI GCANNNNNTGC 1 cut(s) 175
BstBAI YACGTR 1 cut(s) 142
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 1 cut(s) 53
BstDSI CCRYGG 2 cut(s) 164, 279
BstF5I GGATG 2 cut(s) 145, 273
BstFNI CGCG 4 cut(s) 166, 281, 326, 503
BstH2I RGCGCY 2 cut(s) 84, 347
BstHHI GCGC 3 cut(s) 83, 346, 503
BstKTI GATC 2 cut(s) 232, 597
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 2 cut(s) 229, 594
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 7 cut(s) 172, 175, 287, 290, 320, 352, 480
BstNI CCWGG 1 cut(s) 242
BstSCI CCNGG 6 cut(s) 215, 232, 240, 256, 257, 346
BstSLI GKGCMC 1 cut(s) 525
BstUI CGCG 4 cut(s) 166, 281, 326, 503
BstV1I GCAGC 8 cut(s) 58, 62, 162, 180, 181, 184, 202, 461
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BstZI CGGCCG 1 cut(s) 213
BsuRI GGCC 4 cut(s) 20, 107, 215, 323
BtgI CCRYGG 2 cut(s) 164, 279
BtgZI GCGATG 2 cut(s) 27, 423
BtsCI GGATG 2 cut(s) 145, 273
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 1 cut(s) 210
CaiI CAGNNNCTG 3 cut(s) 52, 175, 196
CfoI GCGC 3 cut(s) 83, 346, 503
Cfr13I GGNCC 2 cut(s) 219, 245
Cfr42I CCGCGG 2 cut(s) 167, 282
Cfr9I CCCGGG 1 cut(s) 257
CseI GACGC 2 cut(s) 315, 361
Csp6I GTAC 2 cut(s) 143, 591
CviAII CATG 2 cut(s) 484, 516
CviQI GTAC 2 cut(s) 143, 591
DdeI CTNAG 1 cut(s) 53
DinI GGCGCC 2 cut(s) 82, 345
DpnI GATC 2 cut(s) 231, 596
DpnII GATC 2 cut(s) 229, 594
EaeI YGGCCR 3 cut(s) 18, 105, 213
EagI CGGCCG 1 cut(s) 213
EciI GGCGGA 3 cut(s) 308, 329, 431
EclXI CGGCCG 1 cut(s) 213
Eco32I GATATC 1 cut(s) 613
Eco47I GGWCC 2 cut(s) 219, 245
Eco52I CGGCCG 1 cut(s) 213
Eco88I CYCGRG 2 cut(s) 257, 599
EcoRII CCWGG 1 cut(s) 240
EcoRV GATATC 1 cut(s) 613
EgeI GGCGCC 2 cut(s) 82, 345
EheI GGCGCC 2 cut(s) 82, 345
Esp3I CGTCTC 1 cut(s) 383
FaeI CATG 2 cut(s) 487, 519
FaiI YATR 3 cut(s) 485, 512, 517
FaqI GGGAC 2 cut(s) 364, 561
FatI CATG 2 cut(s) 483, 515
FokI GGATG 2 cut(s) 132, 280
GlaI GCGC 3 cut(s) 82, 345, 502
GsuI CTGGAG 2 cut(s) 45, 404
HaeII RGCGCY 2 cut(s) 84, 347
HaeIII GGCC 4 cut(s) 20, 107, 215, 323
HapII CCGG 6 cut(s) 63, 216, 233, 248, 258, 347
HgaI GACGC 2 cut(s) 315, 361
HhaI GCGC 3 cut(s) 83, 346, 503
Hin1I GRCGYC 3 cut(s) 81, 344, 353
Hin1II CATG 2 cut(s) 487, 519
Hin6I GCGC 3 cut(s) 81, 344, 501
HinP1I GCGC 3 cut(s) 81, 344, 501
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 6 cut(s) 66, 199, 224, 464, 562, 604
HpaII CCGG 6 cut(s) 63, 216, 233, 248, 258, 347
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 3 cut(s) 223, 229, 620
Hpy188III TCNNGA 3 cut(s) 308, 601, 608
Hpy8I GTNNAC 1 cut(s) 253
Hpy99I CGWCG 3 cut(s) 11, 122, 331
HpyCH4III ACNGT 2 cut(s) 118, 590
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 3 cut(s) 483, 534, 585
HpyF10VI GCNNNNNNNGC 7 cut(s) 172, 175, 287, 290, 320, 352, 480
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 1 cut(s) 141
Hsp92I GRCGYC 3 cut(s) 81, 344, 353
Hsp92II CATG 2 cut(s) 487, 519
HspAI GCGC 3 cut(s) 81, 344, 501
KasI GGCGCC 2 cut(s) 80, 343
KspI CCGCGG 2 cut(s) 167, 282
Kzo9I GATC 2 cut(s) 229, 594
LmnI GCTCC 2 cut(s) 36, 575
Lsp1109I GCAGC 8 cut(s) 58, 62, 162, 180, 181, 184, 202, 461
LweI GCATC 2 cut(s) 4, 400
MaeII ACGT 1 cut(s) 141
MalI GATC 2 cut(s) 231, 596
MboI GATC 2 cut(s) 229, 594
MboII GAAGA 3 cut(s) 143, 505, 607
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 525
Mly113I GGCGCC 2 cut(s) 81, 344
MspA1I CMGCKG 5 cut(s) 49, 166, 175, 193, 281
MspI CCGG 6 cut(s) 63, 216, 233, 248, 258, 347
MspR9I CCNGG 6 cut(s) 217, 234, 242, 258, 259, 348
MvaI CCWGG 1 cut(s) 242
MvnI CGCG 4 cut(s) 166, 281, 326, 503
MwoI GCNNNNNNNGC 7 cut(s) 172, 175, 287, 290, 320, 352, 480
NarI GGCGCC 2 cut(s) 81, 344
NciI CCSGG 5 cut(s) 217, 234, 258, 259, 348
NdeII GATC 2 cut(s) 229, 594
NlaIII CATG 2 cut(s) 487, 519
NlaIV GGNNCC 6 cut(s) 82, 220, 231, 246, 345, 571
PaeR7I CTCGAG 1 cut(s) 599
PcsI WCGNNNNNNNCGW 1 cut(s) 606
PfeI GAWTC 6 cut(s) 66, 199, 224, 464, 562, 604
Pfl23II CGTACG 1 cut(s) 590
PluTI GGCGCC 2 cut(s) 84, 347
Ppu21I YACGTR 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PspLI CGTACG 1 cut(s) 590
PspN4I GGNNCC 6 cut(s) 82, 220, 231, 246, 345, 571
PspPI GGNCC 2 cut(s) 219, 245
PsrI GAACNNNNNNTAC 2 cut(s) 126, 158
PstNI CAGNNNCTG 3 cut(s) 52, 175, 196
PsuI RGATCY 1 cut(s) 229
PvuII CAGCTG 2 cut(s) 175, 193
RsaI GTAC 2 cut(s) 144, 592
RsaNI GTAC 2 cut(s) 143, 591
SacII CCGCGG 2 cut(s) 167, 282
Sau3AI GATC 2 cut(s) 229, 594
Sau96I GGNCC 2 cut(s) 219, 245
ScrFI CCNGG 6 cut(s) 217, 234, 242, 258, 259, 348
SduI GDGCHC 1 cut(s) 525
SetI ASST 7 cut(s) 41, 144, 177, 186, 195, 276, 555
SfaNI GCATC 2 cut(s) 4, 400
SfoI GGCGCC 2 cut(s) 82, 345
Sfr274I CTCGAG 1 cut(s) 599
Sfr303I CCGCGG 2 cut(s) 167, 282
SgrBI CCGCGG 2 cut(s) 167, 282
SinI GGWCC 2 cut(s) 219, 245
SlaI CTCGAG 1 cut(s) 599
SmaI CCCGGG 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
SspDI GGCGCC 2 cut(s) 80, 343
StyD4I CCNGG 6 cut(s) 215, 232, 240, 256, 257, 346
TaaI ACNGT 2 cut(s) 118, 590
TaiI ACGT 1 cut(s) 144
TaqI TCGA 5 cut(s) 120, 545, 565, 600, 609
TfiI GAWTC 6 cut(s) 66, 199, 224, 464, 562, 604
TscAI CASTG 1 cut(s) 28
TseI GCWGC 8 cut(s) 46, 75, 169, 172, 175, 190, 193, 474
TspDTI ATGAA 3 cut(s) 144, 284, 499
TspGWI ACGGA 2 cut(s) 115, 407
TspMI CCCGGG 1 cut(s) 257
TspRI CASTG 1 cut(s) 28
VpaK11BI GGWCC 2 cut(s) 219, 245
XhoI CTCGAG 1 cut(s) 599
XmaI CCCGGG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.