MD10G1328000.v1.1

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007033
Physical Location & Seq
Forward (+)
40744413 .. 40746006
1594 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1328000.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGACGACGCGGGTGAAGCGAGGGGGGTTTTGGGCAGGCGGAGCTTCAAGCAACGGCTGAGACTGAACGGGTTCGGATGCTGTGGGGCCACCTGGGGCTTCGGACGGACGGCGGCGATTGGCGCCAGAGAGGATGAGGAAGATCAAGACCCGCAAAATCTTCCGCCACAAGAACGAGTGGTTATAACTGTGGACCAGGCTGAACCGGTTAATATTTGGAATCCGGATTGCGTTGCTCCGGTTTCGAACGGGTCGGGTATGAATTTAGCTGCCGCTCTGGCAGCCGAGCGACAGTTCCGGGATCCGCAGGATACGGAGGGAGAGGGGTCGGTGGGTCCCGCGGATAATGCAAGGACGGGAACAACGGCGCCGGGAACGCCGATGAGAGTGTCGCTGATGAGGCTGCTTGAGGAGACGGAGATGGACGGCTGCGACGCGTTTGGCGGTGGCGCGACGGCGGACAGTGAAAAAGCAGCGGAGGGAGGGATAGGGAGCGATTCGATGTGCTGCGTGTGCATGGGAAGGAAAAAAGGCGCGGCTTTCATCCCATGTGGGCACACATTTTGCAGGGTGTGTTCGAGGGAGGTGTGGTTGAATCGAGGTTCCTGTCCCCTCTGCAACCGTTCGATCCTCGAGATCCTCGATATATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

218

Amino Acids

23.06

Weight (kDa)

4.74

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 166 - 211 1.1e-11 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 168 - 206 8.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 169 - 206 1.4e-06 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 169 - 210 7.5e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 185
AccB1I GGYRCC 2 cut(s) 122, 367
AccBSI CCGCTC 1 cut(s) 275
AccII CGCG 5 cut(s) 11, 341, 437, 452, 536
AccIII TCCGGA 1 cut(s) 223
AclWI GGATC 4 cut(s) 296, 309, 622, 631
AcsI RAATTY 1 cut(s) 262
AcyI GRCGYC 2 cut(s) 123, 368
AflIII ACRYGT 1 cut(s) 435
AgeI ACCGGT 1 cut(s) 205
AgsI TTSAA 2 cut(s) 49, 595
AjnI CCWGG 2 cut(s) 92, 195
AluBI AGCT 2 cut(s) 45, 269
AluI AGCT 2 cut(s) 45, 269
Alw26I GTCTC 2 cut(s) 55, 407
AlwI GGATC 4 cut(s) 296, 309, 622, 631
Ama87I CYCGRG 1 cut(s) 632
Aor13HI TCCGGA 1 cut(s) 223
AoxI GGCC 1 cut(s) 87
ApeKI GCWGC 6 cut(s) 269, 281, 403, 429, 473, 507
ApoI RAATTY 1 cut(s) 262
AsiGI ACCGGT 1 cut(s) 205
Asp700I GAANNNNTTC 1 cut(s) 71
AspLEI GCGC 4 cut(s) 125, 370, 452, 536
AspS9I GGNCC 3 cut(s) 87, 193, 335
AsuC2I CCSGG 2 cut(s) 299, 372
AsuHPI GGTGA 1 cut(s) 26
AsuII TTCGAA 1 cut(s) 245
AvaI CYCGRG 1 cut(s) 632
AvaII GGWCC 2 cut(s) 193, 335
BaeGI GKGCMC 1 cut(s) 558
BamHI GGATCC 1 cut(s) 301
BanI GGYRCC 2 cut(s) 122, 367
BbvI GCAGC 6 cut(s) 256, 293, 390, 416, 485, 494
BccI CCATC 1 cut(s) 415
BceAI ACGGC 5 cut(s) 71, 126, 381, 442, 471
BciT130I CCWGG 2 cut(s) 94, 197
BciVI GTATCC 1 cut(s) 304
BcnI CCSGG 2 cut(s) 299, 372
BcoDI GTCTC 2 cut(s) 55, 407
BfoI RGCGCY 2 cut(s) 126, 371
BfuI GTATCC 1 cut(s) 304
BglI GCCNNNNNGGC 1 cut(s) 278
BisI GCNGC 9 cut(s) 114, 270, 273, 282, 404, 430, 474, 508, 537
BlsI GCNGC 9 cut(s) 115, 271, 274, 283, 405, 431, 475, 509, 538
Bme1390I CCNGG 4 cut(s) 94, 197, 299, 372
Bme18I GGWCC 2 cut(s) 193, 335
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 3 cut(s) 87, 193, 335
BmiI GGNNCC 7 cut(s) 88, 124, 303, 336, 337, 369, 604
BmrFI CCNGG 4 cut(s) 94, 197, 299, 372
BmsI GCATC 1 cut(s) 68
Bpu14I TTCGAA 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 428
BpuMI CCSGG 2 cut(s) 299, 372
BsaHI GRCGYC 2 cut(s) 123, 368
BsaJI CCNNGG 2 cut(s) 93, 339
BsaWI WCCGGW 3 cut(s) 205, 223, 238
BsaXI ACNNNNNCTCC 2 cut(s) 575, 605
Bse118I RCCGGY 1 cut(s) 205
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 2 cut(s) 94, 197
BseDI CCNNGG 2 cut(s) 93, 339
BseGI GGATG 3 cut(s) 83, 139, 543
BseMII CTCAG 1 cut(s) 50
BseRI GAGGAG 1 cut(s) 425
BseSI GKGCMC 1 cut(s) 558
BseXI GCAGC 6 cut(s) 256, 293, 390, 416, 485, 494
Bsh1236I CGCG 5 cut(s) 11, 341, 437, 452, 536
BshFI GGCC 1 cut(s) 89
BshNI GGYRCC 2 cut(s) 122, 367
BshTI ACCGGT 1 cut(s) 205
BsiHKCI CYCGRG 1 cut(s) 632
BsiSI CCGG 5 cut(s) 206, 224, 239, 298, 371
BslFI GGGAC 2 cut(s) 321, 594
BsmAI GTCTC 2 cut(s) 55, 407
BsmBI CGTCTC 1 cut(s) 407
BsmFI GGGAC 2 cut(s) 321, 594
BsnI GGCC 1 cut(s) 89
BsoBI CYCGRG 1 cut(s) 632
Bsp119I TTCGAA 1 cut(s) 245
Bsp1286I GDGCHC 1 cut(s) 558
Bsp13I TCCGGA 1 cut(s) 223
Bsp143I GATC 4 cut(s) 142, 301, 627, 636
BspANI GGCC 1 cut(s) 89
BspCNI CTCAG 1 cut(s) 51
BspEI TCCGGA 1 cut(s) 223
BspFNI CGCG 5 cut(s) 11, 341, 437, 452, 536
BspLI GGNNCC 7 cut(s) 88, 124, 303, 336, 337, 369, 604
BspPI GGATC 4 cut(s) 296, 309, 622, 631
BspT104I TTCGAA 1 cut(s) 245
BspT107I GGYRCC 2 cut(s) 122, 367
BsrBI CCGCTC 1 cut(s) 275
BsrFI RCCGGY 1 cut(s) 205
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 2 cut(s) 93, 339
BssMI GATC 4 cut(s) 142, 301, 627, 636
BssNI GRCGYC 2 cut(s) 123, 368
Bst2UI CCWGG 2 cut(s) 94, 197
Bst4CI ACNGT 4 cut(s) 190, 294, 464, 623
BstACI GRCGYC 2 cut(s) 123, 368
BstBI TTCGAA 1 cut(s) 245
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 1 cut(s) 59
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 3 cut(s) 83, 139, 543
BstFNI CGCG 5 cut(s) 11, 341, 437, 452, 536
BstH2I RGCGCY 2 cut(s) 126, 371
BstHHI GCGC 4 cut(s) 125, 370, 452, 536
BstKTI GATC 4 cut(s) 145, 304, 630, 639
BstMAI GTCTC 2 cut(s) 55, 407
BstMBI GATC 4 cut(s) 142, 301, 627, 636
BstMWI GCNNNNNNNGC 9 cut(s) 17, 42, 122, 278, 281, 347, 376, 400, 513
BstNI CCWGG 2 cut(s) 94, 197
BstSCI CCNGG 4 cut(s) 92, 195, 297, 370
BstSLI GKGCMC 1 cut(s) 558
BstUI CGCG 5 cut(s) 11, 341, 437, 452, 536
BstV1I GCAGC 6 cut(s) 256, 293, 390, 416, 485, 494
BstX2I RGATCY 2 cut(s) 301, 636
BstYI RGATCY 2 cut(s) 301, 636
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 1 cut(s) 89
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 3 cut(s) 83, 139, 543
BtsIMutI CAGTG 1 cut(s) 469
Cac8I GCNNGC 1 cut(s) 38
CfoI GCGC 4 cut(s) 125, 370, 452, 536
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 3 cut(s) 87, 193, 335
Cfr42I CCGCGG 1 cut(s) 342
CseI GACGC 2 cut(s) 17, 443
CspAI ACCGGT 1 cut(s) 205
CviAII CATG 2 cut(s) 517, 549
DdeI CTNAG 1 cut(s) 59
DinI GGCGCC 2 cut(s) 124, 369
DpnI GATC 4 cut(s) 144, 303, 629, 638
DpnII GATC 4 cut(s) 142, 301, 627, 636
EciI GGCGGA 3 cut(s) 55, 153, 473
Eco47I GGWCC 2 cut(s) 193, 335
Eco88I CYCGRG 1 cut(s) 632
EcoO109I RGGNCCY 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 92, 195
EgeI GGCGCC 2 cut(s) 124, 369
EheI GGCGCC 2 cut(s) 124, 369
Esp3I CGTCTC 1 cut(s) 407
FaeI CATG 2 cut(s) 520, 552
FaiI YATR 5 cut(s) 185, 260, 518, 550, 647
FaqI GGGAC 2 cut(s) 321, 594
FatI CATG 2 cut(s) 516, 548
FauI CCCGC 3 cut(s) 4, 159, 346
Fnu4HI GCNGC 9 cut(s) 114, 270, 273, 282, 404, 430, 474, 508, 537
FokI GGATG 3 cut(s) 90, 146, 530
Fsp4HI GCNGC 9 cut(s) 114, 270, 273, 282, 404, 430, 474, 508, 537
GlaI GCGC 4 cut(s) 124, 369, 451, 535
GluI GCNGC 9 cut(s) 114, 270, 273, 282, 404, 430, 474, 508, 537
HaeII RGCGCY 2 cut(s) 126, 371
HaeIII GGCC 1 cut(s) 89
HapII CCGG 5 cut(s) 206, 224, 239, 298, 371
HgaI GACGC 2 cut(s) 17, 443
HhaI GCGC 4 cut(s) 125, 370, 452, 536
Hin1I GRCGYC 2 cut(s) 123, 368
Hin1II CATG 2 cut(s) 520, 552
Hin6I GCGC 4 cut(s) 123, 368, 450, 534
HinP1I GCGC 4 cut(s) 123, 368, 450, 534
HinfI GANTC 3 cut(s) 220, 497, 595
HpaII CCGG 5 cut(s) 206, 224, 239, 298, 371
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 2 cut(s) 77, 104
Hpy188III TCNNGA 3 cut(s) 146, 224, 634
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 3 cut(s) 11, 437, 457
HpyAV CCTTC 1 cut(s) 516
HpyCH4III ACNGT 4 cut(s) 190, 294, 464, 623
HpyCH4V TGCA 4 cut(s) 350, 516, 567, 618
HpyF10VI GCNNNNNNNGC 9 cut(s) 17, 42, 122, 278, 281, 347, 376, 400, 513
HpyF3I CTNAG 1 cut(s) 59
Hsp92I GRCGYC 2 cut(s) 123, 368
Hsp92II CATG 2 cut(s) 520, 552
HspAI GCGC 4 cut(s) 123, 368, 450, 534
KasI GGCGCC 2 cut(s) 122, 367
KflI GGGWCCC 1 cut(s) 335
Kpn2I TCCGGA 1 cut(s) 223
KspI CCGCGG 1 cut(s) 342
Kzo9I GATC 4 cut(s) 142, 301, 627, 636
LmnI GCTCC 3 cut(s) 42, 241, 492
Lsp1109I GCAGC 6 cut(s) 256, 293, 390, 416, 485, 494
LweI GCATC 1 cut(s) 68
MalI GATC 4 cut(s) 144, 303, 629, 638
MbiI CCGCTC 1 cut(s) 275
MboI GATC 4 cut(s) 142, 301, 627, 636
MboII GAAGA 2 cut(s) 152, 152
MflI RGATCY 2 cut(s) 301, 636
MhlI GDGCHC 1 cut(s) 558
MluCI AATT 1 cut(s) 262
MluI ACGCGT 1 cut(s) 435
Mly113I GGCGCC 2 cut(s) 123, 368
MroI TCCGGA 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 1 cut(s) 210
MspA1I CMGCKG 2 cut(s) 341, 476
MspI CCGG 5 cut(s) 206, 224, 239, 298, 371
MspR9I CCNGG 4 cut(s) 94, 197, 299, 372
MvaI CCWGG 2 cut(s) 94, 197
MvnI CGCG 5 cut(s) 11, 341, 437, 452, 536
MwoI GCNNNNNNNGC 9 cut(s) 17, 42, 122, 278, 281, 347, 376, 400, 513
NarI GGCGCC 2 cut(s) 123, 368
NciI CCSGG 2 cut(s) 299, 372
NdeII GATC 4 cut(s) 142, 301, 627, 636
NlaIII CATG 2 cut(s) 520, 552
NlaIV GGNNCC 7 cut(s) 88, 124, 303, 336, 337, 369, 604
NmeAIII GCCGAG 1 cut(s) 310
NspV TTCGAA 1 cut(s) 245
PaeR7I CTCGAG 1 cut(s) 632
PcsI WCGNNNNNNNCGW 1 cut(s) 639
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 3 cut(s) 220, 497, 595
PfoI TCCNGGA 1 cut(s) 297
PinAI ACCGGT 1 cut(s) 205
PkrI GCNGC 9 cut(s) 115, 271, 274, 283, 405, 431, 475, 509, 538
PluTI GGCGCC 2 cut(s) 126, 371
PpuMI RGGWCCY 1 cut(s) 335
PsiI TTATAA 1 cut(s) 185
Psp5II RGGWCCY 1 cut(s) 335
Psp6I CCWGG 2 cut(s) 92, 195
PspGI CCWGG 2 cut(s) 92, 195
PspN4I GGNNCC 7 cut(s) 88, 124, 303, 336, 337, 369, 604
PspPI GGNCC 3 cut(s) 87, 193, 335
PspPPI RGGWCCY 1 cut(s) 335
PsuI RGATCY 2 cut(s) 301, 636
SacII CCGCGG 1 cut(s) 342
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 9 cut(s) 114, 270, 273, 282, 404, 430, 474, 508, 537
Sau3AI GATC 4 cut(s) 142, 301, 627, 636
Sau96I GGNCC 3 cut(s) 87, 193, 335
ScrFI CCNGG 4 cut(s) 94, 197, 299, 372
SduI GDGCHC 1 cut(s) 558
SetI ASST 5 cut(s) 47, 95, 271, 588, 604
SfaNI GCATC 1 cut(s) 68
SfoI GGCGCC 2 cut(s) 124, 369
Sfr274I CTCGAG 1 cut(s) 632
Sfr303I CCGCGG 1 cut(s) 342
SfuI TTCGAA 1 cut(s) 245
SgrBI CCGCGG 1 cut(s) 342
SinI GGWCC 2 cut(s) 193, 335
SlaI CTCGAG 1 cut(s) 632
SmlI CTYRAG 2 cut(s) 407, 632
SmoI CTYRAG 2 cut(s) 407, 632
Sse9I AATT 1 cut(s) 262
SspDI GGCGCC 2 cut(s) 122, 367
SspI AATATT 1 cut(s) 214
StyD4I CCNGG 4 cut(s) 92, 195, 297, 370
TaaI ACNGT 4 cut(s) 190, 294, 464, 623
TaqI TCGA 7 cut(s) 245, 500, 578, 598, 626, 633, 642
TasI AATT 1 cut(s) 262
TauI GCSGC 3 cut(s) 116, 275, 539
TfiI GAWTC 3 cut(s) 220, 497, 595
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 1 cut(s) 469
TseI GCWGC 6 cut(s) 269, 281, 403, 429, 473, 507
TspDTI ATGAA 2 cut(s) 275, 532
TspGWI ACGGA 3 cut(s) 121, 329, 431
TspRI CASTG 1 cut(s) 469
VpaK11BI GGWCC 2 cut(s) 193, 335
XapI RAATTY 1 cut(s) 262
XhoI CTCGAG 1 cut(s) 632
XmnI GAANNNNTTC 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.