pycom17g07310

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007033
Physical Location & Seq
Forward (+)
5266237 .. 5266959
723 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g07310.1

Sequence Viewer

Length: 723 bp
ATGAGTCAACTAAGTCTGATATTGCAGGAATCGGTGCGGAGGGAGAGGGCGGCAAGGACGGTCCTCGAGGTTCTGAGAGAGCAAATGGACGACGCGGATGGTGCGAGGGGGGTTGTGGGCAGGCGGAGCTTCAAGCAACGGCTGAGACTGAGCGTGTTCGGATGCTGTGGGGCCACCTGGGGATTCGGACGGACGTCGGCGATTGGCGCCAGAGAGGATGAGGAAGATCAAGAACCGCAAAATCTTCCGCCACAAGAACGAGTGGTTATAACTGTGGGCCAGGCTGGACCGGTTAATATTTGGGATCCGGGTTGCGTGGCTCCGGTTTCGCACGGATCGGGTATGAATTTGGCTGCGGCTCTGGCAGCCGAGCGACAGTTGCGGGCTCCTCAGGATCCGGAGGGAGAGGGGTCGGTGGGTCCCACGGGTAATGCAATCGCGGGAACGCCGATGAGAGTGTCGCTGATGAGGCTGATTGAGGAGACGGAGATGGACGGGTGCGACCCGTTTGGCGGTGGCGCGACGGCGTTGAGCGAAAAAACAGCGGAGGGAGGGATAGGGAGCGATTCGGTGTGCTGCGTGTGTATGGGAAGGAAAAAAGGCGCGGCTTTCATCCCGTGTGGGCACACATTTTGCAGGGTGTGTTCGAGAGAGGTGTGGTTGAATCGAGGATCCTGTCCCCTCTGCAACCGTTCGATCCTCGAGGTCCTCGATATATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

241

Amino Acids

25.61

Weight (kDa)

5.39

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 189 - 235 2.4e-11 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 192 - 229 1.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 192 - 229 1.7e-06 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 192 - 233 9.1e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 269
AatII GACGTC 1 cut(s) 197
AbsI CCTCGAGG 2 cut(s) 65, 701
AccB1I GGYRCC 1 cut(s) 206
AccII CGCG 4 cut(s) 95, 440, 521, 605
AccIII TCCGGA 1 cut(s) 397
AclWI GGATC 8 cut(s) 299, 312, 343, 389, 402, 666, 679, 691
AcsI RAATTY 1 cut(s) 346
AcyI GRCGYC 2 cut(s) 194, 207
AfiI CCNNNNNNNGG 1 cut(s) 512
AgeI ACCGGT 1 cut(s) 289
AgsI TTSAA 2 cut(s) 133, 664
AjnI CCWGG 2 cut(s) 176, 279
AluBI AGCT 1 cut(s) 129
AluI AGCT 1 cut(s) 129
Alw26I GTCTC 2 cut(s) 139, 476
AlwI GGATC 8 cut(s) 299, 312, 343, 389, 402, 666, 679, 691
Ama87I CYCGRG 2 cut(s) 65, 701
Aor13HI TCCGGA 1 cut(s) 397
AoxI GGCC 2 cut(s) 171, 277
ApeKI GCWGC 3 cut(s) 353, 365, 576
ApoI RAATTY 1 cut(s) 346
AsiGI ACCGGT 1 cut(s) 289
AspLEI GCGC 3 cut(s) 209, 521, 605
AspS9I GGNCC 6 cut(s) 61, 171, 277, 287, 419, 706
AsuC2I CCSGG 1 cut(s) 309
AvaI CYCGRG 2 cut(s) 65, 701
AvaII GGWCC 4 cut(s) 61, 287, 419, 706
AxyI CCTNAGG 1 cut(s) 390
BaeGI GKGCMC 1 cut(s) 627
BamHI GGATCC 3 cut(s) 304, 394, 671
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 1 cut(s) 388
BbvI GCAGC 3 cut(s) 340, 377, 563
BccI CCATC 2 cut(s) 92, 484
BceAI ACGGC 2 cut(s) 155, 540
BciT130I CCWGG 2 cut(s) 178, 281
BcnI CCSGG 1 cut(s) 309
BcoDI GTCTC 2 cut(s) 139, 476
BfaI CTAG 1 cut(s) 721
BfoI RGCGCY 1 cut(s) 210
BisI GCNGC 6 cut(s) 51, 354, 357, 366, 577, 606
BlsI GCNGC 6 cut(s) 52, 355, 358, 367, 578, 607
Bme1390I CCNGG 3 cut(s) 178, 281, 309
Bme18I GGWCC 4 cut(s) 61, 287, 419, 706
BmeT110I CYCGRG 2 cut(s) 65, 701
BmgT120I GGNCC 6 cut(s) 61, 171, 277, 287, 419, 706
BmiI GGNNCC 9 cut(s) 172, 208, 306, 321, 387, 396, 420, 421, 673
BmrFI CCNGG 3 cut(s) 178, 281, 309
BmsI GCATC 1 cut(s) 152
BoxI GACNNNNGTC 1 cut(s) 193
BpuMI CCSGG 1 cut(s) 309
BsaHI GRCGYC 2 cut(s) 194, 207
BsaJI CCNNGG 2 cut(s) 177, 423
BsaWI WCCGGW 3 cut(s) 289, 322, 397
Bsc4I CCNNNNNNNGG 1 cut(s) 512
Bse118I RCCGGY 1 cut(s) 289
Bse21I CCTNAGG 1 cut(s) 390
BseAI TCCGGA 1 cut(s) 397
BseBI CCWGG 2 cut(s) 178, 281
BseDI CCNNGG 2 cut(s) 177, 423
BseGI GGATG 4 cut(s) 103, 167, 223, 612
BseLI CCNNNNNNNGG 1 cut(s) 512
BseMII CTCAG 4 cut(s) 65, 134, 140, 404
BseRI GAGGAG 2 cut(s) 378, 494
BseSI GKGCMC 1 cut(s) 627
BseXI GCAGC 3 cut(s) 340, 377, 563
Bsh1236I CGCG 4 cut(s) 95, 440, 521, 605
BshFI GGCC 2 cut(s) 173, 279
BshNI GGYRCC 1 cut(s) 206
BshTI ACCGGT 1 cut(s) 289
BsiHKCI CYCGRG 2 cut(s) 65, 701
BsiSI CCGG 4 cut(s) 290, 308, 323, 398
BslFI GGGAC 2 cut(s) 405, 663
BslI CCNNNNNNNGG 1 cut(s) 512
BsmAI GTCTC 2 cut(s) 139, 476
BsmBI CGTCTC 1 cut(s) 476
BsmFI GGGAC 2 cut(s) 405, 663
BsnI GGCC 2 cut(s) 173, 279
BsoBI CYCGRG 2 cut(s) 65, 701
Bsp1286I GDGCHC 2 cut(s) 388, 627
Bsp13I TCCGGA 1 cut(s) 397
Bsp143I GATC 6 cut(s) 226, 304, 335, 394, 671, 696
BspANI GGCC 2 cut(s) 173, 279
BspCNI CTCAG 4 cut(s) 66, 135, 141, 403
BspEI TCCGGA 1 cut(s) 397
BspFNI CGCG 4 cut(s) 95, 440, 521, 605
BspLI GGNNCC 9 cut(s) 172, 208, 306, 321, 387, 396, 420, 421, 673
BspPI GGATC 8 cut(s) 299, 312, 343, 389, 402, 666, 679, 691
BspT107I GGYRCC 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 289
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 2 cut(s) 177, 423
BssMI GATC 6 cut(s) 226, 304, 335, 394, 671, 696
BssNI GRCGYC 2 cut(s) 194, 207
Bst2UI CCWGG 2 cut(s) 178, 281
Bst4CI ACNGT 4 cut(s) 61, 274, 378, 692
BstACI GRCGYC 2 cut(s) 194, 207
BstC8I GCNNGC 2 cut(s) 122, 384
BstDEI CTNAG 5 cut(s) 11, 74, 143, 149, 390
BstDSI CCRYGG 1 cut(s) 423
BstF5I GGATG 4 cut(s) 103, 167, 223, 612
BstFNI CGCG 4 cut(s) 95, 440, 521, 605
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 3 cut(s) 209, 521, 605
BstKTI GATC 6 cut(s) 229, 307, 338, 397, 674, 699
BstMAI GTCTC 2 cut(s) 139, 476
BstMBI GATC 6 cut(s) 226, 304, 335, 394, 671, 696
BstMWI GCNNNNNNNGC 7 cut(s) 101, 126, 206, 362, 365, 379, 469
BstNI CCWGG 2 cut(s) 178, 281
BstPAI GACNNNNGTC 1 cut(s) 193
BstSCI CCNGG 3 cut(s) 176, 279, 307
BstSLI GKGCMC 1 cut(s) 627
BstUI CGCG 4 cut(s) 95, 440, 521, 605
BstV1I GCAGC 3 cut(s) 340, 377, 563
BstX2I RGATCY 3 cut(s) 304, 394, 671
BstYI RGATCY 3 cut(s) 304, 394, 671
Bsu36I CCTNAGG 1 cut(s) 390
BsuRI GGCC 2 cut(s) 173, 279
BtgI CCRYGG 1 cut(s) 423
BtsCI GGATG 4 cut(s) 103, 167, 223, 612
Cac8I GCNNGC 2 cut(s) 122, 384
CfoI GCGC 3 cut(s) 209, 521, 605
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 6 cut(s) 61, 171, 277, 287, 419, 706
CseI GACGC 1 cut(s) 101
CspAI ACCGGT 1 cut(s) 289
DdeI CTNAG 5 cut(s) 11, 74, 143, 149, 390
DinI GGCGCC 1 cut(s) 208
DpnI GATC 6 cut(s) 228, 306, 337, 396, 673, 698
DpnII GATC 6 cut(s) 226, 304, 335, 394, 671, 696
EciI GGCGGA 2 cut(s) 139, 237
Eco24I GRGCYC 1 cut(s) 388
Eco47I GGWCC 4 cut(s) 61, 287, 419, 706
Eco81I CCTNAGG 1 cut(s) 390
Eco88I CYCGRG 2 cut(s) 65, 701
EcoO109I RGGNCCY 2 cut(s) 419, 706
EcoRII CCWGG 2 cut(s) 176, 279
EcoT38I GRGCYC 1 cut(s) 388
EgeI GGCGCC 1 cut(s) 208
EheI GGCGCC 1 cut(s) 208
Esp3I CGTCTC 1 cut(s) 476
FaiI YATR 4 cut(s) 269, 344, 587, 716
FaqI GGGAC 2 cut(s) 405, 663
FauI CCCGC 2 cut(s) 375, 433
Fnu4HI GCNGC 6 cut(s) 51, 354, 357, 366, 577, 606
FokI GGATG 4 cut(s) 110, 174, 230, 599
FriOI GRGCYC 1 cut(s) 388
Fsp4HI GCNGC 6 cut(s) 51, 354, 357, 366, 577, 606
FspBI CTAG 1 cut(s) 721
GlaI GCGC 3 cut(s) 208, 520, 604
GluI GCNGC 6 cut(s) 51, 354, 357, 366, 577, 606
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 2 cut(s) 173, 279
HapII CCGG 4 cut(s) 290, 308, 323, 398
HgaI GACGC 1 cut(s) 101
HhaI GCGC 3 cut(s) 209, 521, 605
Hin1I GRCGYC 2 cut(s) 194, 207
Hin6I GCGC 3 cut(s) 207, 519, 603
HinP1I GCGC 3 cut(s) 207, 519, 603
HincII GTYRAC 1 cut(s) 8
HindII GTYRAC 1 cut(s) 8
HinfI GANTC 5 cut(s) 4, 29, 183, 566, 664
HpaII CCGG 4 cut(s) 290, 308, 323, 398
Hpy166II GTNNAC 1 cut(s) 8
Hpy188I TCNGA 4 cut(s) 18, 75, 161, 188
Hpy188III TCNNGA 4 cut(s) 230, 392, 398, 648
Hpy8I GTNNAC 1 cut(s) 8
Hpy99I CGWCG 3 cut(s) 95, 199, 526
HpyAV CCTTC 1 cut(s) 585
HpyCH4III ACNGT 4 cut(s) 61, 274, 378, 692
HpyCH4IV ACGT 1 cut(s) 194
HpyCH4V TGCA 4 cut(s) 25, 434, 636, 687
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 126, 206, 362, 365, 379, 469
HpyF3I CTNAG 5 cut(s) 11, 74, 143, 149, 390
HpySE526I ACGT 1 cut(s) 194
Hsp92I GRCGYC 2 cut(s) 194, 207
HspAI GCGC 3 cut(s) 207, 519, 603
KasI GGCGCC 1 cut(s) 206
KflI GGGWCCC 1 cut(s) 419
Kpn2I TCCGGA 1 cut(s) 397
Kzo9I GATC 6 cut(s) 226, 304, 335, 394, 671, 696
LmnI GCTCC 4 cut(s) 126, 325, 391, 561
Lsp1109I GCAGC 3 cut(s) 340, 377, 563
LweI GCATC 1 cut(s) 152
MaeI CTAG 1 cut(s) 721
MaeII ACGT 1 cut(s) 194
MalI GATC 6 cut(s) 228, 306, 337, 396, 673, 698
MboI GATC 6 cut(s) 226, 304, 335, 394, 671, 696
MboII GAAGA 2 cut(s) 236, 236
MflI RGATCY 3 cut(s) 304, 394, 671
MhlI GDGCHC 2 cut(s) 388, 627
MluCI AATT 1 cut(s) 346
Mly113I GGCGCC 1 cut(s) 207
MlyI GAGTC 1 cut(s) 13
MroI TCCGGA 1 cut(s) 397
MseI TTAA 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 4 cut(s) 290, 308, 323, 398
MspR9I CCNGG 3 cut(s) 178, 281, 309
MvaI CCWGG 2 cut(s) 178, 281
MvnI CGCG 4 cut(s) 95, 440, 521, 605
MwoI GCNNNNNNNGC 7 cut(s) 101, 126, 206, 362, 365, 379, 469
NarI GGCGCC 1 cut(s) 207
NciI CCSGG 1 cut(s) 309
NdeII GATC 6 cut(s) 226, 304, 335, 394, 671, 696
NlaIV GGNNCC 9 cut(s) 172, 208, 306, 321, 387, 396, 420, 421, 673
NmeAIII GCCGAG 1 cut(s) 394
PaeR7I CTCGAG 2 cut(s) 65, 701
PcsI WCGNNNNNNNCGW 1 cut(s) 708
PfeI GAWTC 4 cut(s) 29, 183, 566, 664
PinAI ACCGGT 1 cut(s) 289
PkrI GCNGC 6 cut(s) 52, 355, 358, 367, 578, 607
PleI GAGTC 1 cut(s) 12
PluTI GGCGCC 1 cut(s) 210
PpsI GAGTC 1 cut(s) 12
PpuMI RGGWCCY 2 cut(s) 419, 706
PshAI GACNNNNGTC 1 cut(s) 193
PsiI TTATAA 1 cut(s) 269
Psp5II RGGWCCY 2 cut(s) 419, 706
Psp6I CCWGG 2 cut(s) 176, 279
PspGI CCWGG 2 cut(s) 176, 279
PspN4I GGNNCC 9 cut(s) 172, 208, 306, 321, 387, 396, 420, 421, 673
PspPI GGNCC 6 cut(s) 61, 171, 277, 287, 419, 706
PspPPI RGGWCCY 2 cut(s) 419, 706
PspXI VCTCGAGB 2 cut(s) 65, 701
PsuI RGATCY 3 cut(s) 304, 394, 671
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 6 cut(s) 51, 354, 357, 366, 577, 606
Sau3AI GATC 6 cut(s) 226, 304, 335, 394, 671, 696
Sau96I GGNCC 6 cut(s) 61, 171, 277, 287, 419, 706
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 3 cut(s) 178, 281, 309
SduI GDGCHC 2 cut(s) 388, 627
SetI ASST 6 cut(s) 72, 131, 179, 197, 657, 708
SfaNI GCATC 1 cut(s) 152
SfoI GGCGCC 1 cut(s) 208
Sfr274I CTCGAG 2 cut(s) 65, 701
SinI GGWCC 4 cut(s) 61, 287, 419, 706
SlaI CTCGAG 2 cut(s) 65, 701
SmlI CTYRAG 2 cut(s) 65, 701
SmoI CTYRAG 2 cut(s) 65, 701
Sse9I AATT 1 cut(s) 346
SspDI GGCGCC 1 cut(s) 206
SspI AATATT 1 cut(s) 298
SspMI CTAG 1 cut(s) 721
StyD4I CCNGG 3 cut(s) 176, 279, 307
TaaI ACNGT 4 cut(s) 61, 274, 378, 692
TaiI ACGT 1 cut(s) 197
TaqI TCGA 6 cut(s) 66, 647, 667, 695, 702, 711
TasI AATT 1 cut(s) 346
TauI GCSGC 3 cut(s) 53, 359, 608
TfiI GAWTC 4 cut(s) 29, 183, 566, 664
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TseI GCWGC 3 cut(s) 353, 365, 576
TspDTI ATGAA 2 cut(s) 359, 601
TspGWI ACGGA 3 cut(s) 205, 348, 500
VpaK11BI GGWCC 4 cut(s) 61, 287, 419, 706
XapI RAATTY 1 cut(s) 346
XhoI CTCGAG 2 cut(s) 65, 701
XspI CTAG 1 cut(s) 721
ZraI GACGTC 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.